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Papers by Usman Aliyu Dutsinma

Human T-lymphotrophic virus type 1 (HTLV-1) is a causative agent of tropic spastic paraparesis an... more Human T-lymphotrophic virus type 1 (HTLV-1) is a causative agent of tropic spastic paraparesis and adult T-cell leukaemia, Evidence is accumulating that HTLV-1 may be responsible for some degree of subclinical immune suppression that may results in increased rate of HIV. Sixty (60) people living with HIV/AIDS consisting of 20 males and 40 females were recruited in this study. HIV diagnosis was confirmed using Nigerian National Serial Algorithm for HIV test. ELISA technique was used for the detection of HTLV-1 IgG and IgM antibodies, and Cyflow partec was used for CD4 count. The prevalence of HTLV-1 IgG and IgM antibodies among HIV subjects was 15% and 6.6% respectively. Male patients have a percentage prevalence of 4(6.6%) and female 5(8.3%) of IgG antibodies, IgM antibodies prevalence was 2(3.3%) for male and female each respectively. CD4 Counts of the HIV subjects was evaluated which reveals that patients with counts 0-200cells/µl tested negative to HTLV-1 IgG and IgM antibodies. Conclusion: The percentage prevalence recorded in this study shows that HTLV-1 infection is relatively high compared to the previous studies even though limited information was obtained in relation to HIV/HTLV-1 co-infection in these study area.

Infectious Diseases: Prevention and Control, 2019

Bayero Journal of Pure and Applied Sciences, 2020

Respiratory infection is highly prevalent in HIV-infected patients and S. pneumoniae was a leadin... more Respiratory infection is highly prevalent in HIV-infected patients and S. pneumoniae was a leading cause. The aim of this study was to isolate and identify Streptococcus pneumoniae associated with pulmonary infection among HIV Clients Attending Aminu Kano Teaching Hospital (AKTH). A total of 300 samples comprising sample from 180 HIV seropositive patients and 120 HIV seronegative patients as a (control) were enrolled in this study.) For the 180 HIV seropositive patients, 105(58.3%) were females, and 75(41.7%) were males. Among the HIV negative subjects 64(53.3%) were males and 56(46.7%) were females. The incident of Streptococcus pneumoniae isolates associated with pneumonia in HIV/AIDS seropositive patients in relation to age group in this study showed the higher prevalence of 15(51.72%), among the age groups of 30-39, had the highest of prevalence of 15(51.72%), which occur more common in HIV infected patients while for HIV seronegative patients age group 10-19 had the ...

African Journal of Clinical and Experimental Microbiology, Jun 6, 2018

Asian Journal of Medical and Biological Research, 2018

This study was carried out to investigate the drug resistance pattern of Salmonella spp. from dia... more This study was carried out to investigate the drug resistance pattern of Salmonella spp. from diarrheal patients of under 5 years of age. Collected stool samples from the hospitalized diarrheal patients were analyzed for the presence of Salmonella spp. Among 350 stool samples from diarrheal patients, 15 (4%) were positive for the Salmonella spp. Antibiotic susceptibility test of the identified Salmonella spp. was performed according to Kirby-Bauer disc diffusion method. Eight commonly used antibiotics including azithromycin (15 μg), chloramphenicol (30 μg), Sulphamethaxole-trimethoprim (25 μg), metronidazole (50 μg), tetracycline (30 μg), doxycycline (30 μg), erythromycin (15 μg) and ciprofloxacin (5 μg) used to determine drug resistance pattern of the identified Salmonella spp. Majority of the isolates were multidrug resistant showed resistance against more than three drugs. 73% of Salmonella spp. was resistant to 2-4 drugs and 20% showed resistance to more than 5 drugs. We found t...

Mediterranean Journal of Infection Microbes and Antimicrobials, 2022

Introduction: Antimicrobial resistance among Enterobacteriaceae members has progressed from multi... more Introduction: Antimicrobial resistance among Enterobacteriaceae members has progressed from multi to extensively drug resistance status, limiting treatment options for immune-compromised patients (ICPs). Applying mono therapy has proved unsuccessful in many cases; thereby necessitating the need for combination therapy for optimal treatment. Materials and Methods: We studied the incidence of extensively drug resistant (XDR) pathogens among ICPs, screened them for β-lactamase production and determine the effectiveness of combination of antibiotics against the XDR Enterobacteriaceae isolated from a Federal Medical Center in Nigeria using standard CLSI methods. Checkerboard assay was used to determine synergy between Piperacillin-Tazobactam (TZP)/Amoxicillin-Clavulanic (AMC) acid and each of ceftazidime (CAZ), ceftriaxone (CRO) and gentamicin (GN) by fractional inhibitory concentration indices. Results: Sixty-eight Enterobacteriaceae members were isolated and 15 (22.1%) were XDR. Of the 68 isolates, 53.3%, 13.3%, and 0% were ESBL, AmpC and carbapenemase producers respectively. Resistance to meropenem was expressed by 37.5% XDR E. coli, 60% K. pneumoniae and 100% by the only Enterobacter aerogene. Equally alarming is colistin resistance by 50% XDR E. coli and 20% K. pneumoniae. Mono antibiotics with favourable activities against the XDR Enterobacteriaceae were colistin, tigecycline, and meropenem. Synergy was observed for XDR E. coli and K. pneumoniae when TZP was combined with CAZ and CRO. No synergy was observed when AMC was combined with either CAZ or GN. Conclusion: The study demonstrates the incidence of XDR Enterobacteriaceae among ICPs and suggests that TZP plus CAZ or CRO may be a useful combination for the treatment of infections due to XDR Enterobacteriaceae including co-producers of ESBL and AmpC β-lactamase.

Indian Journal of Gynecologic Oncology, 2021

Purpose One of the leading causes of sexually transmitted diseases and other mucosal and epitheli... more Purpose One of the leading causes of sexually transmitted diseases and other mucosal and epithelial-associated diseases is human papillomavirus, especially type 16 and 18. African women with normal cytology have been reported to have the highest prevalence of HPV and also higher tendency of developing invasive cervical cancer due to certain sociocultural and economic factors. Despite this, a cost-effective therapy (vaccine) for human papillomavirus is yet to be produced. Body HPV vaccination (with prophylactic HPV vaccines) is targeted towards preventing infection, but the public health goal is preventing cervical cancer. However, these vaccines had been found not to be effective in eliminating pre-existing lesions, not effective in clearing established HPV infections, and are very expensive especially in low-income countries; thus, the need for therapeutic HPV vaccines that are geared towards preventing low-grade lesion from progressing, controlling the spread of metastatic cancer, regressing existing lesion and finally preventing recurrence of cancer following treatment is highly sought. Conclusion Therapeutic vaccines that broadly target oncogenic HPV types at an early stage, and that are inexpensive, are urgently required.

Acta Scientific Microbiology, 2020

Olayemi and Usman Aliyu Dutsinma. Rotavirus is the most common cause of diarrhoeal disease among ... more Olayemi and Usman Aliyu Dutsinma. Rotavirus is the most common cause of diarrhoeal disease among infants and young children. It is a genus of double-stranded RNA viruses in the family Reoviridae. According to literatures, nearly every child in the world is infected with rotavirus at least once by the age of five. The study was carried out to determine the incidence of Rotavirus among children (0-5years) suffering from diarrhoea attending Hasiya Bayero Pediatric Hospital, Kano state. Two hundred and ninety-nine children within ages 0-5 years who were at Hasiya Bayero Pediatric Hospital, Kano, at the time of the study were enrolled in the study. Diarrhoea samples were obtained following parental consent and ethical approval from the medical research ethics committee of the hospital. The stool samples were collected aseptically in sterile commercial stool containers adequately labeled (patient ID and date of collection) and were transported in ice to the Center for Biotechnology Research, Bayero University Kano where they we restored at −200C until tested. The presence of Rotavirus was detected using RDT and ELISA (IgM) in the stool samples. Rotavirus (IgM) was detected in 22 of the 299 subjects giving an incidence of 7.4% using ELISA. The RDT kit detected Rotavirus in 45 of the 299 subjects giving an incidence of 15.1%. Female subjects had higher incidence (51.1%) compared to male subjects (48.9%). Subjects in age group 13-24 months old had the highest incidence of Rotavirus (5.0% and 3.7%) while subjects in age group 25-36 months old had the lowest percent positive (0.7% and 0.3%) for RDT and ELISA respectively. The socio-demographic variables associated with the incidence of Rotavirus in this study were mother's level of education, breastfeeding practices and method of water storage. ELISA test kit was found to be more specific and sensitive when compared with Rapid test kit used in this study in detecting Rotavirus. It is recommended that mothers should be educated through their anti-natal and post-natal clinics on the process of avoiding the infection through effective hygiene especially when babies are between 0-5years old especially 13-24 months and the detection of Rotavirus among diarrhoea patients should focus more on the use of ELISA instead of RDT.

Novel research in Microbiology journal, 2020

This study was designed to determine the antibiotic resistance pattern of Salmonella spp. isolate... more This study was designed to determine the antibiotic resistance pattern of Salmonella spp. isolated from clinical samples of symptomatic and asymptomatic typhoid patients, selected from some hospitals in Jigawa State, Nigeria. Four hundred bacterial isolates were recovered from these samples, were identified as; Salmonella typhimurium, S. enteritidis, S. typhi, S. paratyphi A, and S. paratyphi B using microgen biochemical system, according to the standard techniques. The antibiogram of all these Salmonella spp. were carried out using standard oxoid disc of different antibiotics. Double disc diffusion technique was employed to determine the isolates' capacity to produce β-lactamase. Results showed 5(1.25%), 0(0.0%), 0(0.0%) of the samples collected were positive for extended spectrum β-lactamases (ESBL), AmpC β-lactamase, and Metallo β-lactamases (MBL), respectively. The antibiogram profile of the isolates showed that 100% of these isolates are susceptible to Imipenem, only 3% exhibited resistance to ceftriaxone, whereas 7% demonstrated resistance to ciprofloxacin. To the best of our knowledge, this the first study of this kind in the Jigawa state, especially on these Salmonella spp. The study concluded that β-lactamases with the capacity of conferring multidrug resistance were recorded in Salmonella spp. isolated from patients in several hospitals of Jigawa State, Nigeria.