VÁclav Stejskal - Academia.edu (original) (raw)
Papers by VÁclav Stejskal
International journal of biological macromolecules, 2018
The order Psocodea which has incorporated the two former orders Psocoptera (barklice and booklice... more The order Psocodea which has incorporated the two former orders Psocoptera (barklice and booklice) and Phthiraptera (parasitic lice) attracts much attention for its unusual mitochondrial (mt) genome rearrangements. Available phylogenetic analysis for Psocodea is subjected to partial taxa and a complete one is needed. To further explore the genome rearrangement and phylogeny in Psocodea, we sequenced the mt genomes of two barklice, Lepinotus reticulatus (collected from China) and Dorypteryx domestica (collected from Czech Republic). Both of newly sequenced barklice had typical one-chromosome mt genomes and the same mt gene arrangement with the reported Lepidopsocidae sp. The mt genomes of L. reticulatus and D. domestica contained 37 genes typical of bilateral animals. In contrast with the recent report mt genome of D. domestica, our strain was found with many single nucleotide polymorphisms in intra-specific difference. Phylogenetic relationships were inferred from all available mt g...
G3 (Bethesda, Md.), Mar 19, 2018
The booklouse, Liposcelis bostrychophila is an important storage pest worldwide. The mt genome of... more The booklouse, Liposcelis bostrychophila is an important storage pest worldwide. The mt genome of an asexual strain (Beibei, China) of the booklouse, L. bostrychophila, comprises two chromosomes; each chromosome contains approximate half of the 37 genes typically found in animals. The mt genomes of two sexual strains of L. bostrychophila, however, comprise five and seven chromosomes respectively; each chromosome contains one to six genes. To understand mt genome evolution in L. bostrychophila and whether L. bostrychophila is a cryptic species, we sequenced the mt genomes of six strains of asexual L. bostrychophila collected from different locations in China, Croatia and USA. The mt genomes of all of the six asexual strains of L. bostrychophila collected in China, Croatia and USA have two chromosomes. Phylogenetic analysis of mt genome sequences divided nine strains of L. bostrychophila into four groups. Each group has a distinct mt genome organization and substantial sequence diverg...
Scientific reports, 2017
Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damag... more Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damage stored products and cause serious economic loss. However, because of the body size (~1 mm) of eggs, nymphs, and adults, morphological identification of most stored-product psocids is difficult and hampers effective identification. In this study, 10 economically important stored-product Liposcelis spp. psocids (Liposcelis brunnea, L. entomophila, L. decolor, L. pearmani, L. rufa, L.mendax, L. bostrychophila, L. corrodens, L. paeta, and L. tricolor) were collected from 25 geographic locations in 3 countries (China, Czech Republic, and the United States). Ten species-specific probes for identifying these 10 psocid species were designed based on ITS2 sequences. The microarray method and reaction system were optimized. Specificity of each of the ten probes was tested, and all probes were found suitable for use in identification of the respective10 Liposcelis spp. psocids at 66 °C. This meth...
Journal of Stored Products Research, 2016
Scientific reports, Jun 29, 2016
Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored pro... more Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored product pests in China and worldwide. They are often found or are intercepted in grain depots, flour mills, and entry-exit ports, etc. Traditionally, Tribolium species are identified according to the morphological characteristics of the adult. However, it is almost impossible to rapidly identify adult fragments and non-adult stages based on external morphological characteristics. Molecular techniques for the rapid and accurate identification of Tribolium species are required, particularly for pest monitoring and the quarantine of stored products pests. Here, we establish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six stored-product pest Tribolium species including T. castaneum, T. confusum, T. destructor, T. madens, T. freemani and T. brevicornis. We detected the mitochondrial DNA cytochrome oxidase subunit I (COI) barcodes for Tribolium from...
Pest Management Science, 2012
The authors investigated whether fluorescent pigment in thermoset melamine microcapsules incorpor... more The authors investigated whether fluorescent pigment in thermoset melamine microcapsules incorporated into monitoring baits would be excreted in the faeces of wild house mice in a quantity and intensity that would be detectable by a human observer. Experimental mice produced 24-116 UV-visible faecal pellets per 24 h; the mean dry weight was 582 mg. The number and weight of the faeces was independent of mouse sex and weight. The defecation of UV-visible faeces began at 2-3 h, peaked at 5-8 h and was complete at 17 h after bait ingestion. The detectability of the highly fluorescent faecal pellets using a small UV flashlight approached 100%, and no false positives were recorded. The tested formulation is of significant value for rodent pest monitoring because faeces that are highly visible by UV light are produced for 15 h by mice after ingestion, and their detection is easy and unambiguous.
Journal of Stored Products Research, 2013
ABSTRACT Psocids of the genus Liposcelis (Psocodea: Liposcelididae) are common economically impor... more ABSTRACT Psocids of the genus Liposcelis (Psocodea: Liposcelididae) are common economically important pests in storage facilities all over the world. Stored-product psocids have small bodies and are difficult to identify to species using external morphological characteristics. Quick and accurate identification methods for psocid species serve as the foundation for pest management, quality control of food products, and settling of legal disputes from quarantine inspection. Liposcelis corrodens (Heymons) is a common storedproduct pest distributed in European, American, and Oceania countries but has not been reported as established in China. To facilitate pest management and quarantine inspection to prevent importation and establishment of L. corrodens in China, methods for accurate and rapid identification need to be developed. Based on the sequencing and alignment of mitochondrial 16S rDNA and COI gene of 10 common Liposcelis species, two pairs of species-specific primers were designed according to the variation regions among species. PCR cycling parameters were developed for the amplification of specific fragments of 16S rDNA and COI genes of L. corrodens with lengths of 261 bp and 243 bp, respectively. Both primer sets have high sensitivity in target species detection and successfully identified psocids found by quarantine personnel in China as L. corrodens. The diagnostic method we have developed is reliable for identification of L. corrodens for quarantine and pest management purposes.
Journal of Stored Products Research, 2010
ABSTRACT The external egg morphology of two stored-product pests, Stegobium paniceum and Lasioder... more ABSTRACT The external egg morphology of two stored-product pests, Stegobium paniceum and Lasioderma serricorne, is presented herein based on optical and scanning electron microscope micrographs. The diagnostic characteristics of the eggs of the pests, including the presence or absence of a micropyle, are described and discussed. Overall, several notable differences were observed between the eggs of these species in regard to their shape, size and surface structures.
Journal of Stored Products Research, 2008
Journal of Stored Products Research, 1995
Journal of Stored Products Research, 2012
Journal of Pest Science, 2014
Entomologia Experimentalis et Applicata, 2010
ABSTRACT Cockroaches (Blattaria) are considered universally gregarious insects. In this study, we... more ABSTRACT Cockroaches (Blattaria) are considered universally gregarious insects. In this study, we tested the hypothesis that even related species and/or males and females of the same species may exhibit different aggregation patterns. We compared aggregation behaviour in six cockroach species belonging to three groups: Eublaberus, Blaberus, and Gromphadorhina-Princisia (all Blaberidae). For comparison, binary choice and free aggregation arena tests were used. The results revealed that blaberid clades clearly differed in their aggregation patterns. Females of Eublaberus species aggregated, whereas only a low aggregation tendency was recorded in conspecific males. Both sexes of Blaberus species aggregated, especially when tested in the arena. The Madagascar hissing cockroaches of the Gromphadorhina-Princisia clade are not gregarious – the aggregation tendency was entirely absent in males of the two species studied and in females of Princisia vanwaerebeki van Herrewege, and was only weakly demonstrated in females of Gromphadorhina portentosa (Schaum). The observed patterns of aggregation are compared with previous reports concerning social behaviour of blaberid species.
Entomologia Experimentalis et Applicata, 1997
Behavioural Processes, 2010
We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-bor... more We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-born house mice: (1) non-commensal Mus musculus domesticus from the eastern part of Syria along the Euphrates River; and (2) commensal M. m. musculus from the Czech Republic. Pair-choice preference tests have revealed that oestrous females of both populations sniffed towards unfamiliar males more than familiar males. In the case of females exhibiting postpartum oestrus, this preference was less pronounced and statistically not significant. Thus, our mice clearly exhibited the behavioural pattern known from commensal populations of polygynous and/or promiscuous M. m. domesticus. We found no inverse tendency to seek proximity to the familiar male that has been previously reported from closely related and presumably monogamous aboriginal mouse Mus spicilegus. We conclude that neither commensal M. m. musculus, nor non-commensal M. m. domesticus, are likely to share a monogamous mating system with mound-building mice.
Sustainability
At a global scale, the sustainability of forests is endangered by multiple invasive species, incl... more At a global scale, the sustainability of forests is endangered by multiple invasive species, including the pine wood nematode (Bursaphelenchus xylophilus), a quarantine pest. International laws and standards require that all exported wood coming from countries in which B. xylophilus is present be chemically or physically treated. Since a major fumigant, methyl bromide, was banned, there has been a need to generate data for alternative fumigants, such as ethanedinitrile (EDN), for this purpose. Since the field screening of fumigants (i.e., the application of various doses to and exposure times of naturally infested wood logs) is prohibitively expensive, the aim of this study was to develop a quick and inexpensive laboratory method. Here, we suggest and describe an innovative method based on sawdust cultures for EDN efficacy screening. In the validation part of this study, we demonstrated (i) the high survival of the nematodes in the sawdust and (ii) the high efficacy of EDN against t...
Behavioural Processes, 2010
We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-bor... more We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-born house mice: (1) non-commensal Mus musculus domesticus from the eastern part of Syria along the Euphrates River; and (2) commensal M. m. musculus from the Czech Republic. Pair-choice preference tests have revealed that oestrous females of both populations sniffed towards unfamiliar males more than familiar males. In the case of females exhibiting postpartum oestrus, this preference was less pronounced and statistically not significant. Thus, our mice clearly exhibited the behavioural pattern known from commensal populations of polygynous and/or promiscuous M. m. domesticus. We found no inverse tendency to seek proximity to the familiar male that has been previously reported from closely related and presumably monogamous aboriginal mouse Mus spicilegus. We conclude that neither commensal M. m. musculus, nor non-commensal M. m. domesticus, are likely to share a monogamous mating system with mound-building mice.
International Journal of Pest Management, 2010
... a , Václav Stejskal a * , Radek Aulický a & Daniel Frynta b pages 35-40. ... 2002... more ... a , Václav Stejskal a * , Radek Aulický a & Daniel Frynta b pages 35-40. ... 200233. Moncho-Bogani, J, Lanuza, E, Hernández, A, Novejarque, A and Martínez-García, F. 2002. Attractive properties of sexual pheromones in mice: innate or learned?. Physiol Behav. , 77: 167176. ...
![Research paper thumbnail of Corrigendum to “PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae)” [J. Stored Prod. Res. 49 (2012) 184–188]](https://attachments.academia-assets.com/43767733/thumbnails/1.jpg)
](Journal of Stored Products Research, 2012
International journal of biological macromolecules, 2018
The order Psocodea which has incorporated the two former orders Psocoptera (barklice and booklice... more The order Psocodea which has incorporated the two former orders Psocoptera (barklice and booklice) and Phthiraptera (parasitic lice) attracts much attention for its unusual mitochondrial (mt) genome rearrangements. Available phylogenetic analysis for Psocodea is subjected to partial taxa and a complete one is needed. To further explore the genome rearrangement and phylogeny in Psocodea, we sequenced the mt genomes of two barklice, Lepinotus reticulatus (collected from China) and Dorypteryx domestica (collected from Czech Republic). Both of newly sequenced barklice had typical one-chromosome mt genomes and the same mt gene arrangement with the reported Lepidopsocidae sp. The mt genomes of L. reticulatus and D. domestica contained 37 genes typical of bilateral animals. In contrast with the recent report mt genome of D. domestica, our strain was found with many single nucleotide polymorphisms in intra-specific difference. Phylogenetic relationships were inferred from all available mt g...
G3 (Bethesda, Md.), Mar 19, 2018
The booklouse, Liposcelis bostrychophila is an important storage pest worldwide. The mt genome of... more The booklouse, Liposcelis bostrychophila is an important storage pest worldwide. The mt genome of an asexual strain (Beibei, China) of the booklouse, L. bostrychophila, comprises two chromosomes; each chromosome contains approximate half of the 37 genes typically found in animals. The mt genomes of two sexual strains of L. bostrychophila, however, comprise five and seven chromosomes respectively; each chromosome contains one to six genes. To understand mt genome evolution in L. bostrychophila and whether L. bostrychophila is a cryptic species, we sequenced the mt genomes of six strains of asexual L. bostrychophila collected from different locations in China, Croatia and USA. The mt genomes of all of the six asexual strains of L. bostrychophila collected in China, Croatia and USA have two chromosomes. Phylogenetic analysis of mt genome sequences divided nine strains of L. bostrychophila into four groups. Each group has a distinct mt genome organization and substantial sequence diverg...
Scientific reports, 2017
Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damag... more Stored-product psocids (Psocoptera: Liposcelididae) are cosmopolitan storage pests that can damage stored products and cause serious economic loss. However, because of the body size (~1 mm) of eggs, nymphs, and adults, morphological identification of most stored-product psocids is difficult and hampers effective identification. In this study, 10 economically important stored-product Liposcelis spp. psocids (Liposcelis brunnea, L. entomophila, L. decolor, L. pearmani, L. rufa, L.mendax, L. bostrychophila, L. corrodens, L. paeta, and L. tricolor) were collected from 25 geographic locations in 3 countries (China, Czech Republic, and the United States). Ten species-specific probes for identifying these 10 psocid species were designed based on ITS2 sequences. The microarray method and reaction system were optimized. Specificity of each of the ten probes was tested, and all probes were found suitable for use in identification of the respective10 Liposcelis spp. psocids at 66 °C. This meth...
Journal of Stored Products Research, 2016
Scientific reports, Jun 29, 2016
Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored pro... more Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored product pests in China and worldwide. They are often found or are intercepted in grain depots, flour mills, and entry-exit ports, etc. Traditionally, Tribolium species are identified according to the morphological characteristics of the adult. However, it is almost impossible to rapidly identify adult fragments and non-adult stages based on external morphological characteristics. Molecular techniques for the rapid and accurate identification of Tribolium species are required, particularly for pest monitoring and the quarantine of stored products pests. Here, we establish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six stored-product pest Tribolium species including T. castaneum, T. confusum, T. destructor, T. madens, T. freemani and T. brevicornis. We detected the mitochondrial DNA cytochrome oxidase subunit I (COI) barcodes for Tribolium from...
Pest Management Science, 2012
The authors investigated whether fluorescent pigment in thermoset melamine microcapsules incorpor... more The authors investigated whether fluorescent pigment in thermoset melamine microcapsules incorporated into monitoring baits would be excreted in the faeces of wild house mice in a quantity and intensity that would be detectable by a human observer. Experimental mice produced 24-116 UV-visible faecal pellets per 24 h; the mean dry weight was 582 mg. The number and weight of the faeces was independent of mouse sex and weight. The defecation of UV-visible faeces began at 2-3 h, peaked at 5-8 h and was complete at 17 h after bait ingestion. The detectability of the highly fluorescent faecal pellets using a small UV flashlight approached 100%, and no false positives were recorded. The tested formulation is of significant value for rodent pest monitoring because faeces that are highly visible by UV light are produced for 15 h by mice after ingestion, and their detection is easy and unambiguous.
Journal of Stored Products Research, 2013
ABSTRACT Psocids of the genus Liposcelis (Psocodea: Liposcelididae) are common economically impor... more ABSTRACT Psocids of the genus Liposcelis (Psocodea: Liposcelididae) are common economically important pests in storage facilities all over the world. Stored-product psocids have small bodies and are difficult to identify to species using external morphological characteristics. Quick and accurate identification methods for psocid species serve as the foundation for pest management, quality control of food products, and settling of legal disputes from quarantine inspection. Liposcelis corrodens (Heymons) is a common storedproduct pest distributed in European, American, and Oceania countries but has not been reported as established in China. To facilitate pest management and quarantine inspection to prevent importation and establishment of L. corrodens in China, methods for accurate and rapid identification need to be developed. Based on the sequencing and alignment of mitochondrial 16S rDNA and COI gene of 10 common Liposcelis species, two pairs of species-specific primers were designed according to the variation regions among species. PCR cycling parameters were developed for the amplification of specific fragments of 16S rDNA and COI genes of L. corrodens with lengths of 261 bp and 243 bp, respectively. Both primer sets have high sensitivity in target species detection and successfully identified psocids found by quarantine personnel in China as L. corrodens. The diagnostic method we have developed is reliable for identification of L. corrodens for quarantine and pest management purposes.
Journal of Stored Products Research, 2010
ABSTRACT The external egg morphology of two stored-product pests, Stegobium paniceum and Lasioder... more ABSTRACT The external egg morphology of two stored-product pests, Stegobium paniceum and Lasioderma serricorne, is presented herein based on optical and scanning electron microscope micrographs. The diagnostic characteristics of the eggs of the pests, including the presence or absence of a micropyle, are described and discussed. Overall, several notable differences were observed between the eggs of these species in regard to their shape, size and surface structures.
Journal of Stored Products Research, 2008
Journal of Stored Products Research, 1995
Journal of Stored Products Research, 2012
Journal of Pest Science, 2014
Entomologia Experimentalis et Applicata, 2010
ABSTRACT Cockroaches (Blattaria) are considered universally gregarious insects. In this study, we... more ABSTRACT Cockroaches (Blattaria) are considered universally gregarious insects. In this study, we tested the hypothesis that even related species and/or males and females of the same species may exhibit different aggregation patterns. We compared aggregation behaviour in six cockroach species belonging to three groups: Eublaberus, Blaberus, and Gromphadorhina-Princisia (all Blaberidae). For comparison, binary choice and free aggregation arena tests were used. The results revealed that blaberid clades clearly differed in their aggregation patterns. Females of Eublaberus species aggregated, whereas only a low aggregation tendency was recorded in conspecific males. Both sexes of Blaberus species aggregated, especially when tested in the arena. The Madagascar hissing cockroaches of the Gromphadorhina-Princisia clade are not gregarious – the aggregation tendency was entirely absent in males of the two species studied and in females of Princisia vanwaerebeki van Herrewege, and was only weakly demonstrated in females of Gromphadorhina portentosa (Schaum). The observed patterns of aggregation are compared with previous reports concerning social behaviour of blaberid species.
Entomologia Experimentalis et Applicata, 1997
Behavioural Processes, 2010
We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-bor... more We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-born house mice: (1) non-commensal Mus musculus domesticus from the eastern part of Syria along the Euphrates River; and (2) commensal M. m. musculus from the Czech Republic. Pair-choice preference tests have revealed that oestrous females of both populations sniffed towards unfamiliar males more than familiar males. In the case of females exhibiting postpartum oestrus, this preference was less pronounced and statistically not significant. Thus, our mice clearly exhibited the behavioural pattern known from commensal populations of polygynous and/or promiscuous M. m. domesticus. We found no inverse tendency to seek proximity to the familiar male that has been previously reported from closely related and presumably monogamous aboriginal mouse Mus spicilegus. We conclude that neither commensal M. m. musculus, nor non-commensal M. m. domesticus, are likely to share a monogamous mating system with mound-building mice.
Sustainability
At a global scale, the sustainability of forests is endangered by multiple invasive species, incl... more At a global scale, the sustainability of forests is endangered by multiple invasive species, including the pine wood nematode (Bursaphelenchus xylophilus), a quarantine pest. International laws and standards require that all exported wood coming from countries in which B. xylophilus is present be chemically or physically treated. Since a major fumigant, methyl bromide, was banned, there has been a need to generate data for alternative fumigants, such as ethanedinitrile (EDN), for this purpose. Since the field screening of fumigants (i.e., the application of various doses to and exposure times of naturally infested wood logs) is prohibitively expensive, the aim of this study was to develop a quick and inexpensive laboratory method. Here, we suggest and describe an innovative method based on sawdust cultures for EDN efficacy screening. In the validation part of this study, we demonstrated (i) the high survival of the nematodes in the sawdust and (ii) the high efficacy of EDN against t...
Behavioural Processes, 2010
We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-bor... more We studied female preferences for familiar and unfamiliar males. The subjects were laboratory-born house mice: (1) non-commensal Mus musculus domesticus from the eastern part of Syria along the Euphrates River; and (2) commensal M. m. musculus from the Czech Republic. Pair-choice preference tests have revealed that oestrous females of both populations sniffed towards unfamiliar males more than familiar males. In the case of females exhibiting postpartum oestrus, this preference was less pronounced and statistically not significant. Thus, our mice clearly exhibited the behavioural pattern known from commensal populations of polygynous and/or promiscuous M. m. domesticus. We found no inverse tendency to seek proximity to the familiar male that has been previously reported from closely related and presumably monogamous aboriginal mouse Mus spicilegus. We conclude that neither commensal M. m. musculus, nor non-commensal M. m. domesticus, are likely to share a monogamous mating system with mound-building mice.
International Journal of Pest Management, 2010
... a , Václav Stejskal a * , Radek Aulický a & Daniel Frynta b pages 35-40. ... 2002... more ... a , Václav Stejskal a * , Radek Aulický a & Daniel Frynta b pages 35-40. ... 200233. Moncho-Bogani, J, Lanuza, E, Hernández, A, Novejarque, A and Martínez-García, F. 2002. Attractive properties of sexual pheromones in mice: innate or learned?. Physiol Behav. , 77: 167176. ...
Journal of Stored Products Research, 2012