VANITHA REDDY M - Academia.edu (original) (raw)
Papers by VANITHA REDDY M
One of the greatest risk factors for cardiovascular diseases is hypercholesterolemia. Moringa ole... more One of the greatest risk factors for cardiovascular diseases is hypercholesterolemia. Moringa oleifera is a good source of phytochemicals and is well explored for its antioxidant properties. Methods:The main aim of the present study was to assess the potential cholesterol lowering effect of Moringa oleifera leaf polyphenols (MOP) in an animal model. Five groups of male Wister rats were fed for 45 days as follows: a standard diet (GI); high fat-cholesterol diet (GII); high fat-cholesterol with MOP (100 and 200mg/kg body wt GIII & GIV respectively); and high fat-cholesterol with statins (Atorvastatin) (G-V). Results:Administration of MOP rich extract (GII and GIV) significantly (p≤0.05) lowered the serum cholesterol, triglycerides and low-density lipoprotein cholesterol. A significant (p≤0.05) decrease in the activity of the HMG CoA reductase enzyme was observed in GIII, GIV and GV but not in GI & GII. Conclusion: The results demonstrate that the polyphenol extract of Moringa oleifera...
Morus indica- MI-S36 is a potential source of phytochemicals and is well explored for its medicin... more Morus indica- MI-S36 is a potential source of phytochemicals and is well explored for its medicinal properties such as antidiabetic and antioxidant activity. In the present study, Mous indica aqueous (MAq) and dechlorophyllised (MDc) extracts efficacy to protect against the carbon tetrachloride (CCl4) induced hepatotoxicity was studied in rats in comparison with standard drug Liv52. The experimental group rats were pre treated with MAq, MDc and Liv52 for 7days. Healthy control and positive control group animals were fed wth Olive oil and after 7days two doses of CCl4 was given at 12 and 36 hrs intervals to all the animal groups. After 12 hrs of CCl4 dosage animals were sacrificed and biochemical parameters analysed in the serum. The total protein, albumin, urea, creatinine, total bilirubin were within the normal level in all the groups whereas the hepatic enzymes AST, ALT and ALP activity was less in Liv52 and Morus treated groups. Total cholesterol, triglycerides, were determined. ...
Plants rich in antioxidants and phytochemicals especially phenolic groups play a protective role ... more Plants rich in antioxidants and phytochemicals especially phenolic groups play a protective role in health and disease conditions, like cancer, diabetes, heart disease hypertension etc. by counteracting reactive oxygen species and inhibiting the lipid oxidation. Morus indica is non-toxic natural therapeutic plant used as herbal medicine as hypoglycemic, hypotensive, and diuretic properties. In the present study proximate, phytochemical and antioxidant profile in dehydrated leaves powder of Morus indica varieties, M5, V1 and S36 were estimated. The 100 methanol, 80 methanol (methanol & water, 8: 2), dechlorophyllised, aqueous cold and hot extracts were prepared and their polyphenol content, antioxidant activity of inhibiting oxidation in oil emulsion and liver microsomes was evaluated at 300 to 500ìg. There was no significant (PMethanol> 80 methanol > Aq. c old and hot. In oil emulsion, no significant difference in inhibiting oxidation was observed in methanol and 80 methanol extracts, whereas in dechlorophylised, aq. cold and hot extracts V1 and S36 showed higher activity. In microsomes, S36 variety was more potent in inhibiting oxidation in all extracts except Aq. cold. S36 was found to be good source of phytochemicals and showed more potency in inhibiting the oxidation in two food and biological systems
Article can be accessed online on: www.ijpsr.com ABSTRACT: Plants rich in antioxidants and phytoc... more Article can be accessed online on: www.ijpsr.com ABSTRACT: Plants rich in antioxidants and phytochemicals especially phenolic groups play a protective role in health and disease conditions, like cancer, diabetes, heart disease hypertension etc. by counteracting reactive oxygen species and inhibiting the lipid oxidation. Morus indica is non-toxic natural therapeutic plant used as herbal medicine as hypoglycemic, hypotensive, and diuretic properties. In the present study proximate, phytochemical and antioxidant profile in dehydrated leaves powder of Morus indica varieties, M5, V1 and S36 were estimated. The 100 % methanol, 80 % methanol (methanol & water, 8: 2), dechlorophyllised, aqueous cold and hot extracts were prepared and their polyphenol content, antioxidant activity of inhibiting oxidation in oil emulsion and liver microsomes was evaluated at 300 to 500µg. There
Annals of Phytomedicine: An International Journal, 2018
Binding of bile acids and increasing their fecal excretion have been hypothesized as a possible m... more Binding of bile acids and increasing their fecal excretion have been hypothesized as a possible mechanism to lower cholesterol. Also, bile acid binding potential has been related to lowering the risk of heart disease and that of cancer. In the present study, leaves of three varieties of Morus indica, viz., M5, V1 and S36 were studied for the bile acid binding capacity (BABC) at different concentrations (200, 400 and 600 mg) and compared with cholestyramine, diosgenin, xanthan gum, guar gum and wheat bran. The BABC was determined by hydration and dialysis diffusion method. The total fiber content was in the order of V1 > M5 > S36. The saponins content ranged from 103 to 136 mg/g extract. The bile acid retardation index (BARI) of the samples was dose dependent and heat treatment improved the BARI significantly (p 0.05). Among varieties, BARI of M5 at 400 mg (89 .24 %) was significantly higher (p 0.05) than VI (6 5.23 %) and S36 (61 .24 %). In comparison with standards, BARI of M5 was higher than guar gum and wheat bran and comparable with cholestyramine and xanthan gum. The BARI of all the three Morus varieties was significantly higher (p 0.05) than that of wheat bran. The results indicated Morus possess better BARI than standards and can be promoted as natural source of saponin and fiber expressing the mechanisms for the lipid lowering properties.
Annals of Phytomedicine : An International Journal, 2016
Medicinal plants are nature's fighters against several degenerative diseases. The reverse pharmac... more Medicinal plants are nature's fighters against several degenerative diseases. The reverse pharmacological approach focuses on traditional knowledge on the usage of medicinal plants for various diseases and revalidating the effect using modern techniques. The Western ghats of Southern India has sheltered a wide range of medicinal plants. The objective of this research work was to evaluate the oxidation inhibiting ability of aqueous extract of four medicinal plants from the Western ghats, namely; Andrographis paniculata (Burm.f.) Wall. ex Nees, Costus speciosus (Koen.) Sm., Canthium parviflorum Lam. and Abrus precatorius L. in biological lipid substrates, viz., cholesterol, low-density lipoprotein and brain homogenate. The IC 50 values of the plant extracts were calculated and the correlation between the phytochemicals and the antioxidant effect exhibited was studied. From the results, it was observed that, the inhibition of oxidation by the aqueous extract of all the four medicinal plants, followed the order cholesterol >LDL> brain homogenate. C. parviflorum exhibited highest antioxidant activity in cholesterol (94.8%) and brain (37.35%) than compared to other medicinal plants. In LDL, the highest antioxidant activity (73.75%) was exhibited by C. speciosus. The antioxidant activity of CP and CS in the substrates correlated well with the polyphenol and flavonoid content of the extracts. The IC 50 values of CS in cholesterol (305 µg, r 2 = 0.9972) was the least among all the medicinal plants. The overall protective action in the substrates was higher in C. parviflorum than other medicinal plants. Thus, the selected medicinal plants can be further explored for its biological activity and utilized as sources of natural antioxidants and phytochemicals against various oxidative stress related diseases.
Annals of Phytomedicine : An International Journal, 2016
Morus indica L. (MI-S36) is a potent source of phytochemicals and its antioxidant activity at in ... more Morus indica L. (MI-S36) is a potent source of phytochemicals and its antioxidant activity at in vitro and ex vivo level is very well studied. Further to proceed with the animal studies, the safety and efficacy of the different solvent extracts has to be evaluated at maximum dosage, i.e., 2 g/kg body weight (BW) as per Organisation for Economic Cooperation and Development (OECD) guidelines.The acute toxicity study recorded no mortality or any toxic reaction in any group after 14 d. of extra cts administration at 2 g/kg BW. The extracts aqueous-MAq; dechlorophylised-MDc; 80% methanol-M8M did not cause any behavioral or physical changes in experimental rats. There was no significant (p 0.05) difference in the biochemical parameters, analysed between the groups. Slight elevation in activity of alanine transaminase (ALT) and alkaline phosphatase (ALP) in MDc treated groups was observed, but did not exert any deleterious effect on the normal metabolism which was supported by the histopathology of liver. Extracts did not induce any oxidative stress which is indicated by, no significant difference (p 0.05) in the control and experimental lipid peroxides (LPO) and glutathione values. The serum total cholesterol and triglycerides were less than the control group. Histopathological studies showed no remarkable changes in hepatocytes after 14 d. of oral administration of MAq, MDc and M8M extracts. The study contributes in establishing the non-toxic quality parameters of M. indica leaf and the results indicate the non toxic effect of the extracts.
International Journal of Pharma and Bio Sciences
ABSTRACT
International Journal of Medicinal Chemistry, 2014
Natural antioxidants present in foods and other biological materials have attracted considerable ... more Natural antioxidants present in foods and other biological materials have attracted considerable interest because of their presumed safety and potential nutritional and therapeutic effects. Antioxidant constituents of plant materials act as radical scavengers and convert the radicals to less reactive species.Abrus precatorius(AP) was analyzed for its proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, andin vitroinhibition of Fenton’s reagent-induced oxidation in oil emulsion and microsomes. In addition, the effect of temperature (100∘C, 15, and 30 min) and pH (4.5, 7, and 9) C on the antioxidant activity of ME was investigated. The leaves were rich in total polyphenols, flavonoids,β-carotene, glutathione,α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose-dependent manner. The AP exhibited more...
Journal of Herbs, Spices & Medicinal Plants, 2014
Moringa oleifera root (dehydrated) powder, extracts-aqueous (WE), acetone (AE) and methanol (ME) ... more Moringa oleifera root (dehydrated) powder, extracts-aqueous (WE), acetone (AE) and methanol (ME) were analyzed for phytochemical and antioxidant components by various in vitro assays. The root was found to be a good source of phytochemicals viz, glutathione, a-Tocopherol polyphenols, tannins, alkaloids, saponins. All three solvent extracts exhibited a dose dependent antioxidant activity. The antioxidant properties were established on free radicals; its ability to reduce iron and FeSO 4-induced microsomal lipid peroxidation. All three extracts were able to protect DNA from oxidative damage. The suggestive antioxidant mechanism of action of Moringa oleifera is by scavenging reactive oxygen species.
Journal of Food Science and Technology, 2013
Unrefined groundnut oil (GNO) and refined sunflower oil (SFO) were blended with four minor oils i... more Unrefined groundnut oil (GNO) and refined sunflower oil (SFO) were blended with four minor oils including laboratory refined red palmolein (RRPO), physically refined rice bran oil (RBO), unrefined sesame oil (SESO), and unrefined coconut oil (CNO) containing natural antioxidants viz., β-carotene, tocopherols, oryzanol and lignans. The five blends prepared were GNO+RRPO (80:20), GNO+RBO (80:20), GNO+SESO (80:20), SFO +RRPO (50:50) and SFO +CNO (60:40). Prepared blends contained saturated fatty acids (SFA) (16.7-53.3 %); monounsaturated fatty acids (MUFA) (16.0-45.5 %) and polyunsaturated fatty acids (PUFA) (29.2-37.8 %). GNO blends viz., GNO+RRPO, GNO+RBO and GNO+SESO contained βcarotene (10.7 mg/100 g), oryzanol (0.12 g/100 g) and lignans (0.35 g/100 g) respectively as natural antioxidants. SFO was enriched with β-carotene (28.7 mg/100 g) and medium chain fatty acids (34.2 %) by blending with RRPO and CNO respectively. The oil blends (200 ml) were packed and stored at 38°C/90 % relative humidity (RH) and 27°C/65 % RH and samples were withdrawn at fixed intervals for analysis. Freshly prepared blends were also investigated for their frying performance. During storage, GNO+RBO blend showed highest oxidative stability probably due to the presence of oryzanol in the order GNO+RBO>GNO+SESO>GNO+RRPO. During frying, the peroxide value of GNO blends with RBO (rich in oryzanol) and SESO (rich in lignans) was less while the free fatty acid value was less in SFO blends with RRPO and CNO. Hence, blending of natural antioxidants rich minor oils (RRPO, RBO and SESO) with the major vegetable oils (GNO and SFO) may preserve them by lowering their rate of oxidation during storage and frying.
Journal of Food Science and Technology, 2011
Aegle marmelos (AM) leaves were extracted with methanol (ME), ethanol (EE), water (WE) and analyz... more Aegle marmelos (AM) leaves were extracted with methanol (ME), ethanol (EE), water (WE) and analyzed for antioxidant activities by DPPH radical scavenging method, reducing power and in vitro inhibition by Fenton's reagent-induced oxidation of lipid system. Stability of extracts to pH (4, 7 and 9) and temperature (100°C, 15 min.) was studied. The three extracts showed varying degree of efficacy in each assay in a dose dependent manner. The inhibition of MDA formation in Linseed oil by EE (47%) was significantly (P<0.05) higher than WE (28%) and ME (23%) but less than α-Tocopherol (80%). WE showed maximum stability to high temperature. The antioxidant activity of EE at pH 4 was significantly higher (P<0.05) compared with WE and ME. At pH 7, the antioxidant activity of all the three extracts remained unchanged. Data indicates that potential exists for the utilization of Aegle marmelos as a natural antioxidant.
Food Chemistry, 2005
Natural antioxidants have gained considerable interest in recent years for their role in preventi... more Natural antioxidants have gained considerable interest in recent years for their role in preventing the auto oxidation of fats, oils and fat containing food products. In the present study, three plant foods viz., amla (Emblica officianalis), drumstick leaves (Moringa oleifera) and raisins (Vitis vinifera) were used as sources of natural antioxidants. All the three extracts exhibited a high percentage of antioxidant activity evaluated using b-carotene-linoleic acid in vitro system, compared to synthetic antioxidants. Biscuits prepared by addition of natural extracts were subjected to sensory studies and chemical analysis. Biscuits treated with natural antioxidants, extracted from raisins (B4) and drumstick leaves (B5) received higher (P 6 0.05) panel scores during storage period of 6 weeks, than control (B1), butylated hydroxyl anisole (BHA) (B2) and amla (B3) extract incorporated biscuits. Addition of plant extracts from the three plant foods gave an excellent antioxidant effect on the biscuit compared with the effect of BHA, as the % increase in both peroxide and acid values after 6 weeks were lower than that of the control and BHA treated samples. Extracts from drumstick leaves and amla were more effective in controlling lipid oxidation during storage.
One of the greatest risk factors for cardiovascular diseases is hypercholesterolemia. Moringa ole... more One of the greatest risk factors for cardiovascular diseases is hypercholesterolemia. Moringa oleifera is a good source of phytochemicals and is well explored for its antioxidant properties. Methods:The main aim of the present study was to assess the potential cholesterol lowering effect of Moringa oleifera leaf polyphenols (MOP) in an animal model. Five groups of male Wister rats were fed for 45 days as follows: a standard diet (GI); high fat-cholesterol diet (GII); high fat-cholesterol with MOP (100 and 200mg/kg body wt GIII & GIV respectively); and high fat-cholesterol with statins (Atorvastatin) (G-V). Results:Administration of MOP rich extract (GII and GIV) significantly (p≤0.05) lowered the serum cholesterol, triglycerides and low-density lipoprotein cholesterol. A significant (p≤0.05) decrease in the activity of the HMG CoA reductase enzyme was observed in GIII, GIV and GV but not in GI & GII. Conclusion: The results demonstrate that the polyphenol extract of Moringa oleifera...
Morus indica- MI-S36 is a potential source of phytochemicals and is well explored for its medicin... more Morus indica- MI-S36 is a potential source of phytochemicals and is well explored for its medicinal properties such as antidiabetic and antioxidant activity. In the present study, Mous indica aqueous (MAq) and dechlorophyllised (MDc) extracts efficacy to protect against the carbon tetrachloride (CCl4) induced hepatotoxicity was studied in rats in comparison with standard drug Liv52. The experimental group rats were pre treated with MAq, MDc and Liv52 for 7days. Healthy control and positive control group animals were fed wth Olive oil and after 7days two doses of CCl4 was given at 12 and 36 hrs intervals to all the animal groups. After 12 hrs of CCl4 dosage animals were sacrificed and biochemical parameters analysed in the serum. The total protein, albumin, urea, creatinine, total bilirubin were within the normal level in all the groups whereas the hepatic enzymes AST, ALT and ALP activity was less in Liv52 and Morus treated groups. Total cholesterol, triglycerides, were determined. ...
Plants rich in antioxidants and phytochemicals especially phenolic groups play a protective role ... more Plants rich in antioxidants and phytochemicals especially phenolic groups play a protective role in health and disease conditions, like cancer, diabetes, heart disease hypertension etc. by counteracting reactive oxygen species and inhibiting the lipid oxidation. Morus indica is non-toxic natural therapeutic plant used as herbal medicine as hypoglycemic, hypotensive, and diuretic properties. In the present study proximate, phytochemical and antioxidant profile in dehydrated leaves powder of Morus indica varieties, M5, V1 and S36 were estimated. The 100 methanol, 80 methanol (methanol & water, 8: 2), dechlorophyllised, aqueous cold and hot extracts were prepared and their polyphenol content, antioxidant activity of inhibiting oxidation in oil emulsion and liver microsomes was evaluated at 300 to 500ìg. There was no significant (PMethanol> 80 methanol > Aq. c old and hot. In oil emulsion, no significant difference in inhibiting oxidation was observed in methanol and 80 methanol extracts, whereas in dechlorophylised, aq. cold and hot extracts V1 and S36 showed higher activity. In microsomes, S36 variety was more potent in inhibiting oxidation in all extracts except Aq. cold. S36 was found to be good source of phytochemicals and showed more potency in inhibiting the oxidation in two food and biological systems
Article can be accessed online on: www.ijpsr.com ABSTRACT: Plants rich in antioxidants and phytoc... more Article can be accessed online on: www.ijpsr.com ABSTRACT: Plants rich in antioxidants and phytochemicals especially phenolic groups play a protective role in health and disease conditions, like cancer, diabetes, heart disease hypertension etc. by counteracting reactive oxygen species and inhibiting the lipid oxidation. Morus indica is non-toxic natural therapeutic plant used as herbal medicine as hypoglycemic, hypotensive, and diuretic properties. In the present study proximate, phytochemical and antioxidant profile in dehydrated leaves powder of Morus indica varieties, M5, V1 and S36 were estimated. The 100 % methanol, 80 % methanol (methanol & water, 8: 2), dechlorophyllised, aqueous cold and hot extracts were prepared and their polyphenol content, antioxidant activity of inhibiting oxidation in oil emulsion and liver microsomes was evaluated at 300 to 500µg. There
Annals of Phytomedicine: An International Journal, 2018
Binding of bile acids and increasing their fecal excretion have been hypothesized as a possible m... more Binding of bile acids and increasing their fecal excretion have been hypothesized as a possible mechanism to lower cholesterol. Also, bile acid binding potential has been related to lowering the risk of heart disease and that of cancer. In the present study, leaves of three varieties of Morus indica, viz., M5, V1 and S36 were studied for the bile acid binding capacity (BABC) at different concentrations (200, 400 and 600 mg) and compared with cholestyramine, diosgenin, xanthan gum, guar gum and wheat bran. The BABC was determined by hydration and dialysis diffusion method. The total fiber content was in the order of V1 > M5 > S36. The saponins content ranged from 103 to 136 mg/g extract. The bile acid retardation index (BARI) of the samples was dose dependent and heat treatment improved the BARI significantly (p 0.05). Among varieties, BARI of M5 at 400 mg (89 .24 %) was significantly higher (p 0.05) than VI (6 5.23 %) and S36 (61 .24 %). In comparison with standards, BARI of M5 was higher than guar gum and wheat bran and comparable with cholestyramine and xanthan gum. The BARI of all the three Morus varieties was significantly higher (p 0.05) than that of wheat bran. The results indicated Morus possess better BARI than standards and can be promoted as natural source of saponin and fiber expressing the mechanisms for the lipid lowering properties.
Annals of Phytomedicine : An International Journal, 2016
Medicinal plants are nature's fighters against several degenerative diseases. The reverse pharmac... more Medicinal plants are nature's fighters against several degenerative diseases. The reverse pharmacological approach focuses on traditional knowledge on the usage of medicinal plants for various diseases and revalidating the effect using modern techniques. The Western ghats of Southern India has sheltered a wide range of medicinal plants. The objective of this research work was to evaluate the oxidation inhibiting ability of aqueous extract of four medicinal plants from the Western ghats, namely; Andrographis paniculata (Burm.f.) Wall. ex Nees, Costus speciosus (Koen.) Sm., Canthium parviflorum Lam. and Abrus precatorius L. in biological lipid substrates, viz., cholesterol, low-density lipoprotein and brain homogenate. The IC 50 values of the plant extracts were calculated and the correlation between the phytochemicals and the antioxidant effect exhibited was studied. From the results, it was observed that, the inhibition of oxidation by the aqueous extract of all the four medicinal plants, followed the order cholesterol >LDL> brain homogenate. C. parviflorum exhibited highest antioxidant activity in cholesterol (94.8%) and brain (37.35%) than compared to other medicinal plants. In LDL, the highest antioxidant activity (73.75%) was exhibited by C. speciosus. The antioxidant activity of CP and CS in the substrates correlated well with the polyphenol and flavonoid content of the extracts. The IC 50 values of CS in cholesterol (305 µg, r 2 = 0.9972) was the least among all the medicinal plants. The overall protective action in the substrates was higher in C. parviflorum than other medicinal plants. Thus, the selected medicinal plants can be further explored for its biological activity and utilized as sources of natural antioxidants and phytochemicals against various oxidative stress related diseases.
Annals of Phytomedicine : An International Journal, 2016
Morus indica L. (MI-S36) is a potent source of phytochemicals and its antioxidant activity at in ... more Morus indica L. (MI-S36) is a potent source of phytochemicals and its antioxidant activity at in vitro and ex vivo level is very well studied. Further to proceed with the animal studies, the safety and efficacy of the different solvent extracts has to be evaluated at maximum dosage, i.e., 2 g/kg body weight (BW) as per Organisation for Economic Cooperation and Development (OECD) guidelines.The acute toxicity study recorded no mortality or any toxic reaction in any group after 14 d. of extra cts administration at 2 g/kg BW. The extracts aqueous-MAq; dechlorophylised-MDc; 80% methanol-M8M did not cause any behavioral or physical changes in experimental rats. There was no significant (p 0.05) difference in the biochemical parameters, analysed between the groups. Slight elevation in activity of alanine transaminase (ALT) and alkaline phosphatase (ALP) in MDc treated groups was observed, but did not exert any deleterious effect on the normal metabolism which was supported by the histopathology of liver. Extracts did not induce any oxidative stress which is indicated by, no significant difference (p 0.05) in the control and experimental lipid peroxides (LPO) and glutathione values. The serum total cholesterol and triglycerides were less than the control group. Histopathological studies showed no remarkable changes in hepatocytes after 14 d. of oral administration of MAq, MDc and M8M extracts. The study contributes in establishing the non-toxic quality parameters of M. indica leaf and the results indicate the non toxic effect of the extracts.
International Journal of Pharma and Bio Sciences
ABSTRACT
International Journal of Medicinal Chemistry, 2014
Natural antioxidants present in foods and other biological materials have attracted considerable ... more Natural antioxidants present in foods and other biological materials have attracted considerable interest because of their presumed safety and potential nutritional and therapeutic effects. Antioxidant constituents of plant materials act as radical scavengers and convert the radicals to less reactive species.Abrus precatorius(AP) was analyzed for its proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, andin vitroinhibition of Fenton’s reagent-induced oxidation in oil emulsion and microsomes. In addition, the effect of temperature (100∘C, 15, and 30 min) and pH (4.5, 7, and 9) C on the antioxidant activity of ME was investigated. The leaves were rich in total polyphenols, flavonoids,β-carotene, glutathione,α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose-dependent manner. The AP exhibited more...
Journal of Herbs, Spices & Medicinal Plants, 2014
Moringa oleifera root (dehydrated) powder, extracts-aqueous (WE), acetone (AE) and methanol (ME) ... more Moringa oleifera root (dehydrated) powder, extracts-aqueous (WE), acetone (AE) and methanol (ME) were analyzed for phytochemical and antioxidant components by various in vitro assays. The root was found to be a good source of phytochemicals viz, glutathione, a-Tocopherol polyphenols, tannins, alkaloids, saponins. All three solvent extracts exhibited a dose dependent antioxidant activity. The antioxidant properties were established on free radicals; its ability to reduce iron and FeSO 4-induced microsomal lipid peroxidation. All three extracts were able to protect DNA from oxidative damage. The suggestive antioxidant mechanism of action of Moringa oleifera is by scavenging reactive oxygen species.
Journal of Food Science and Technology, 2013
Unrefined groundnut oil (GNO) and refined sunflower oil (SFO) were blended with four minor oils i... more Unrefined groundnut oil (GNO) and refined sunflower oil (SFO) were blended with four minor oils including laboratory refined red palmolein (RRPO), physically refined rice bran oil (RBO), unrefined sesame oil (SESO), and unrefined coconut oil (CNO) containing natural antioxidants viz., β-carotene, tocopherols, oryzanol and lignans. The five blends prepared were GNO+RRPO (80:20), GNO+RBO (80:20), GNO+SESO (80:20), SFO +RRPO (50:50) and SFO +CNO (60:40). Prepared blends contained saturated fatty acids (SFA) (16.7-53.3 %); monounsaturated fatty acids (MUFA) (16.0-45.5 %) and polyunsaturated fatty acids (PUFA) (29.2-37.8 %). GNO blends viz., GNO+RRPO, GNO+RBO and GNO+SESO contained βcarotene (10.7 mg/100 g), oryzanol (0.12 g/100 g) and lignans (0.35 g/100 g) respectively as natural antioxidants. SFO was enriched with β-carotene (28.7 mg/100 g) and medium chain fatty acids (34.2 %) by blending with RRPO and CNO respectively. The oil blends (200 ml) were packed and stored at 38°C/90 % relative humidity (RH) and 27°C/65 % RH and samples were withdrawn at fixed intervals for analysis. Freshly prepared blends were also investigated for their frying performance. During storage, GNO+RBO blend showed highest oxidative stability probably due to the presence of oryzanol in the order GNO+RBO>GNO+SESO>GNO+RRPO. During frying, the peroxide value of GNO blends with RBO (rich in oryzanol) and SESO (rich in lignans) was less while the free fatty acid value was less in SFO blends with RRPO and CNO. Hence, blending of natural antioxidants rich minor oils (RRPO, RBO and SESO) with the major vegetable oils (GNO and SFO) may preserve them by lowering their rate of oxidation during storage and frying.
Journal of Food Science and Technology, 2011
Aegle marmelos (AM) leaves were extracted with methanol (ME), ethanol (EE), water (WE) and analyz... more Aegle marmelos (AM) leaves were extracted with methanol (ME), ethanol (EE), water (WE) and analyzed for antioxidant activities by DPPH radical scavenging method, reducing power and in vitro inhibition by Fenton's reagent-induced oxidation of lipid system. Stability of extracts to pH (4, 7 and 9) and temperature (100°C, 15 min.) was studied. The three extracts showed varying degree of efficacy in each assay in a dose dependent manner. The inhibition of MDA formation in Linseed oil by EE (47%) was significantly (P<0.05) higher than WE (28%) and ME (23%) but less than α-Tocopherol (80%). WE showed maximum stability to high temperature. The antioxidant activity of EE at pH 4 was significantly higher (P<0.05) compared with WE and ME. At pH 7, the antioxidant activity of all the three extracts remained unchanged. Data indicates that potential exists for the utilization of Aegle marmelos as a natural antioxidant.
Food Chemistry, 2005
Natural antioxidants have gained considerable interest in recent years for their role in preventi... more Natural antioxidants have gained considerable interest in recent years for their role in preventing the auto oxidation of fats, oils and fat containing food products. In the present study, three plant foods viz., amla (Emblica officianalis), drumstick leaves (Moringa oleifera) and raisins (Vitis vinifera) were used as sources of natural antioxidants. All the three extracts exhibited a high percentage of antioxidant activity evaluated using b-carotene-linoleic acid in vitro system, compared to synthetic antioxidants. Biscuits prepared by addition of natural extracts were subjected to sensory studies and chemical analysis. Biscuits treated with natural antioxidants, extracted from raisins (B4) and drumstick leaves (B5) received higher (P 6 0.05) panel scores during storage period of 6 weeks, than control (B1), butylated hydroxyl anisole (BHA) (B2) and amla (B3) extract incorporated biscuits. Addition of plant extracts from the three plant foods gave an excellent antioxidant effect on the biscuit compared with the effect of BHA, as the % increase in both peroxide and acid values after 6 weeks were lower than that of the control and BHA treated samples. Extracts from drumstick leaves and amla were more effective in controlling lipid oxidation during storage.