Vitor Loureiro Da Silva - Academia.edu (original) (raw)

Papers by Vitor Loureiro Da Silva

Brazilian Journal of Medical and Biological Research

Sarcoplasmic reticulum Ca 2+-ATPase (SERCA2a) and sarcolemmal Na + /Ca 2+ exchanger (NCX1) struct... more Sarcoplasmic reticulum Ca 2+-ATPase (SERCA2a) and sarcolemmal Na + /Ca 2+ exchanger (NCX1) structures are involved in heart cell Ca 2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca 2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca 2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca 2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca 2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.

Revista Andaluza de Medicina del Deporte, 2022

Aims: The acute adjustments on the contractile function and the intracellular calcium (Ca2+) hand... more Aims: The acute adjustments on the contractile function and the intracellular calcium (Ca2+) handling in the cardiomyocytes of the right ventricle (RV) after an acute aerobic exercise session are not known. Our aim was to investigate the acute responses of the contractile function and the intracellular Ca2+ handling in isolated RV cardiomyocytes after a swimming exercise session. Methods: Ten-week-old female Wistar rats were randomly allocated into two groups: control (C; n = 5) and exercise (Ex; n = 7). It was performed a swimming exercise session for 30 min. with an overload of 4% relative to the body weight attached to the tail. The rats were sacrificed after the exercise session for the analysis of the RV contractile function parameters and the intracellular Ca2+ handling by the isolated cardiomyocyte technique. Results: Body and heart weights, as well as sarcomere length were similar between the groups. Also, it was not observed differences between the groups for RV cardiomyocy...

Arquivos Brasileiros de Cardiologia, 2021

Fundamento: O remodelamento cardíaco patológico se caracteriza por disfunção diastólica e sistóli... more Fundamento: O remodelamento cardíaco patológico se caracteriza por disfunção diastólica e sistólica, levando à insuficiência cardíaca. Neste contexto, o cenário disfuncional do trânsito de cálcio miocárdico (Ca 2+) tem sido pouco estudado. Um modelo experimental de estenose aórtica tem sido extensamente utilizado para aprimorar os conhecimentos sobre os principais mecanismos do remodelamento patológico cardíaco. Objetivo: Entender o processo disfuncional dos principais componentes responsáveis pelo equilíbrio do cálcio miocárdico e sua influência sobre a função cardíaca na insuficiência cardíaca induzida pela estenose aórtica. Métodos: Ratos Wistar de 21 dias de idade foram distribuídos em dois grupos: controle (placebo; n=28) e estenose aórtica (EaO; n=18). A função cardíaca foi analisada com o ecocardiograma, músculo papilar isolado e cardiomiócitos isolados. No ensaio do músculo papilar, SERCA2a e a atividade do canal de Ca 2+ do tipo L foram avaliados. O ensaio de cardiomiócitos isolados avaliou o trânsito de cálcio. A expressão proteica da proteínas do trânsito de cálcio foi analisada com o western blot. Os resultados foram estatisticamente significativos quando p <0,05. Resultados: Os músculos papilares e cardiomiócitos dos corações no grupo EaO demonstraram falhas mecânicas. Os ratos com EaO apresentaram menor tempo de pico do Ca 2+ , menor sensibilidade das miofibrilas do Ca 2+ , prejuízos nos processos de entrada e recaptura de cálcio pelo retículo sarcoplasmático, bem como disfunção no canal de cálcio do tipo L (CCTL). Além disso, os animais com EaO apresentaram maior expressão de SERCA2a, CCTL e trocador de Na + /Ca 2+. Conclusão: Insuficiência cardíaca sistólica e diastólica devido à estenose aórtica supravalvular acarretou comprometimento da entrada de Ca 2+ celular e inibição da recaptura de cálcio pelo retículo sarcoplasmático devido à disfunção no CCTL e SERCA2a, assim como mudanças no trânsito de cálcio e na expressão das principais proteínas responsáveis pela homeostase de Ca 2+ celular. Palavras-chave: Estenose da Valva Aórtica; Insuficiência Cardíaca; Músculos Papilares; Miócitos Cardíacos; Proteínas de Ligação ao Cálcio.

A Insuficiencia Cardiaca (IC) e uma das principais causas de morte na atualidade. Uma maneira de ... more A Insuficiencia Cardiaca (IC) e uma das principais causas de morte na atualidade. Uma maneira de induzir a IC em um rato, para posteriormente estudar medidas que atenuem tal quadro clinico, e simulando uma Estenose Aortica (EAo) por meio da implantacao de um clipe acima de sua valvula aortica. Estudos sugerem que treinamento fisico suaviza os sintomas antecedentes a insuficiencia cardiaca. A fim de comprovar tal hipotese, uma equipe de pesquisadores da Cardiologia Experimental da Faculdade de Medicina de Botucatu vem realizando experimentos (protocolo de aprovacao no CEAU 1138-2015) em que ratos do tipo Wistar sao induzidos a Estenose Aortica e, posteriormente, submetidos a um especifico protocolo de treinamento fisico. Para efeito de comparacao, outros ratos, aqui denominados Sham (sem implantacao do clipe), tambem sao submetidos ao protocolo de treinamento fisico. Portanto, quatro grupos foram formados: EAo (ratos induzidos a estenose aortica); EAoTF (ratos induzidos a estenose ao...

Cellular Physiology and Biochemistry, 2020

This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 I... more This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.

Cellular Physiology and Biochemistry, 2020

This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 I... more This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.

Frontiers in Physiology, 2019

Experimental studies show that the unsaturated high-fat diet-induced obesity promotes vascular al... more Experimental studies show that the unsaturated high-fat diet-induced obesity promotes vascular alterations characterized by improving the endothelial L-arginine/Nitric Oxide (NO) pathway. Leptin seems to be involved in this process, promoting vasodilation via increasing NO bioavailability. The aim of this study was to test the hypothesis that unsaturated high-fat diet-induced obesity does not generate endothelial dysfunction via increasing the vascular leptin/Akt/eNOS signaling. Thirty-day-old male Wistar rats were randomized into two groups: control (C) and obese (Ob). Group C was fed a standard diet, while group Ob was fed an unsaturated high-fat diet for 27 weeks. Adiposity, hormonal and biochemical parameters, and systolic blood pressure were observed. Concentration response curves were performed for leptin or acetylcholine in the presence or absence of Akt and NOS inhibitor. Our results showed that an unsaturated high-fat diet promoted a greater feed efficiency (FE), elevation of body weight and body fat (BF), and an adiposity index, characterizing a model of obesity. However, comorbidities frequently associated with experimental obesity were not visualized, such as glucose intolerance, dyslipidemia and hypertension. The evaluation of the endothelium-dependent relaxation with acetylcholine showed no differences between the C and Ob rats. After NOS inhibition, the response was completely abolished in the Ob group, but not in the C group. Furthermore, Akt inhibition completely blunted vascular relaxation in the C group, but not in the Ob group, which was more sensitive to leptin-induced vascular relaxation. L-NAME incubation abolished the relaxation in both groups at the same level. Although Akt inhibitor pre-incubation reduced the leptin response, group C was more sensitive to its effect. In conclusion, the high-unsaturated fat diet-induced obesity improved the vascular reactivity to leptin and does not generate endothelial dysfunction, possibly by the increase in the vascular sensitivity to leptin and increasing NO bioavailability. Moreover, our results suggest that the increase in NO production occurs through the increase in NOS activation by leptin and is partially mediated by the Akt pathway.

Revista Brasileira de Medicina do Esporte, 2019

Introduction: Studies indicate that the technical/tactical performance of soccer referees can be ... more Introduction: Studies indicate that the technical/tactical performance of soccer referees can be impacted by physical fitness and state of health. However, although they are routinely invited to take part in matches at national level, it is not yet clearly established whether referees who work in less prominent regions and soccer championships present the same levels of physical, technical and psychological preparation as referees from more important areas, such as the soccer teams of São Paulo and Rio de Janeiro. Within this context, no studies have evaluated the physical conditioning and health parameters of Brazilian Soccer Confederation (CBF) referees who work in Espírito Santo. Objective: To assess the health and physical fitness levels of CBF soccer referees from Espírito Santo. Methods: A cross-sectional study with 16 participants, 6 referees, and 10 assistant referees. The protocol lasted 12 weeks and consisted of the following analyses: general characteristics, body composi...

Pathophysiology : the official journal of the International Society for Pathophysiology, Jan 8, 2018

Cardiac remodeling (CR) is a structural change of the heart due to chronic hemodynamic overload r... more Cardiac remodeling (CR) is a structural change of the heart due to chronic hemodynamic overload related to changes in both myocyte and extracellular matrix (ECM). We investigated that the imbalance of collagen V promotes cardiomyocyte apoptosis that contributes to heart failure and cell death. Aortic stenosis was induced surgically and male Wistar rats were randomized to 18 weeks (Sham 18 w, n = 12; AoS 18 w, n = 12) and severe of heart failure (Sham HF, n = 12; AoS HF, n = 12) groups. Functional and structural echocardiogram, immunohistochemistry for Ki-67, TUNEL assay and Immunofluorescence for collagen were performed. Our main results were: (1) Progressive reduction of cardiac functional capacity due to cardiac remodeling with decreased eject fraction in heart failure; (2) Imbalance of collagen deposition with increased, crowded and irregular collagen I in situ expression; (3) Dysregulation of dynamic control of collagen fibers with exposed epitopes of collagen V; (4) Additional ...

AIDS research and human retroviruses, Jan 6, 2018

HIV infection is characterized by impairment of various epithelial protective mechanisms, factors... more HIV infection is characterized by impairment of various epithelial protective mechanisms, factors that contribute to intestinal barrier damages and result in microbial translocation, leading to increased immune activation and inflammation. However, microbial translocation and inflammatory status in HIV infection has been poorly investigated during pregnancy, period that the women are susceptible to bacterial infections. We aimed to evaluate microbial translocation and the inflammatory status in HIV-infected pregnant women. We studied 30 volunteers, 12 HIV-infected pregnant women (G1), 10 HIV-uninfected pregnant women (G2) and 8 HIV-infected non-pregnant women (G3). The results showed that plasmatic levels of cytokines did not differ among groups. High levels of sCD14 were observed in all groups, with statistical difference in the following comparisons: G1 vs G3 (6727±2030 pg/mL vs 11515±10746 pg/mL; p=0.02) and G2 vs G3 (5256±769 pg/mL vs 11515±10746 pg/mL; p<0.001). Although the...

Physiological reports, 2017

Authors have showed that obesity implicates cardiac dysfunction associated with myocardial L-type... more Authors have showed that obesity implicates cardiac dysfunction associated with myocardial L-type calcium channels (LTCCs) activity impairments, as well as moderate exercise training (MET) seems to be an important therapeutic tool. We tested the hypothesis that MET promotes improvements on LTCCS activity and protein expression at obesity induced by unsaturated high-fat diets, which could represent a protective effects against development of cardiovascular damage. Male Wistar rats were randomized in control (C, n = 40), which received a standard diet and obese (Ob; n = 40), which received high-fat diet. After 20 weeks, the animals were assigned at four groups: control (C; n = 12); control submitted to exercise training (ET; n = 14); obese (Ob; n = 10); and obese submitted to exercise training (ObET; n = 11). ET (5 days/week during 12 weeks) began in the 21th week and consisted of treadmill running that was progressively increased to reach 60 min. Final body weight (FBW), body fat (BF...

ConScientiae Saúde, 2015

Introdução: A obesidade é considerada importante problema de saúde pública e fator de risco para ... more Introdução: A obesidade é considerada importante problema de saúde pública e fator de risco para o desenvolvimento de doenças cardiovasculares. Estudos apontam que o trânsito de cálcio (Ca+2) intracelular e extracelular, mecanismo essencial no acoplamento excitação-contração-relaxamento cardíaco, está envolvido nesse processo patológico. Enquanto o influxo de Ca+2 promove aumento da concentração de Ca+2 livre no citosol na fase de contração, a recaptura e a extrusão do Ca+2 são importantes para a diminuição do Ca+2 intracelular durante o relaxamento. Objetivo: Identificar, baseado na literatura científica, a modulação da disfunção cardíaca pelo trânsito de cálcio em modelos de obesidade genética e dietética. Métodos: A busca de artigos em bases de dados eletrônicas foi realizada com palavras-chaves e seus correspondentes em inglês. Resultados: Inicialmente os artigos que apresentassem uma das palavras-chaves no título foram selecionados. Após processo de triagem, foram identificados...

Medicine & Science in Sports & Exercise, 2015

ConScientiae Saúde, 2015

Introdução: O sistema imunológico apresenta estreita relação com o exercício físico. Todavia, pou... more Introdução: O sistema imunológico apresenta estreita relação com o exercício físico. Todavia, poucos estudos verificaram o perfil imunológico de corredores amadores. Objetivo: Descrever o perfil de marcadores imunológicos de corredores amadores do município de Vitória/ES no período pós-treinamento. Métodos: Foram selecionados 31 corredores pertencentes a dois grupos de corrida, ambos de Vitória/ES. A análise hematológica dos parâmetros imunológicos foi realizada a partir da contagem total de leucócitos por meio de leucograma. Resultados: A contagem de plaquetas apresentou diferença estatística quanto ao gênero, sendo os valores maiores encontrados no gênero feminino em relação ao treinamento contínuo. Além disso, foram encontrados valores menores de neutrófilos no gênero masculino com relação ao treinamento intervalado. Não foi observada diferença estatística entre homens e mulheres nos demais parâmetros analisados. Conclusão: Os resultados deste estudo sugerem que o exercício físic...

Journal of Trainology, 2014

The purpose of this study was to investigate the effects of intensity on rating of perceived exer... more The purpose of this study was to investigate the effects of intensity on rating of perceived exertion (RPE) during multiple sets to volitional failure in bench press exercise. Design and Methods: Thirteen moderately resistance trained men performed 2 experimental sessions in random order: 3 sets to failure in bench press with 1 minute rest at an intensity of 50% and 70% of 1 repetition maximum (1RM). RPE was measured using the OMNI scale after each set. Readiness to Invest in Physical (RTIPE) and Mental (RTIME) Effort were assessed before each set. Results: Repetitions to failure (46.46 ± 9.43 vs 26.62 ± 8.19; P<0.001) and total weight lifted (2044.31 ± 414.5 vs 1650.15 ± 507.83 kg; P<0.001) were greater at 50% when compared to the 70% 1RM condition. There were no differences in RPE, RTIPE, RTIME and heart rate across conditions. Conclusions: These results indicate that when repetitions are done to volitional failure, monitoring RPE is not a useful technique in regulating intensity during resistance training.

Brazilian Journal of Medical and Biological Research

Sarcoplasmic reticulum Ca 2+-ATPase (SERCA2a) and sarcolemmal Na + /Ca 2+ exchanger (NCX1) struct... more Sarcoplasmic reticulum Ca 2+-ATPase (SERCA2a) and sarcolemmal Na + /Ca 2+ exchanger (NCX1) structures are involved in heart cell Ca 2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca 2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca 2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca 2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca 2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.

Revista Andaluza de Medicina del Deporte, 2022

Aims: The acute adjustments on the contractile function and the intracellular calcium (Ca2+) hand... more Aims: The acute adjustments on the contractile function and the intracellular calcium (Ca2+) handling in the cardiomyocytes of the right ventricle (RV) after an acute aerobic exercise session are not known. Our aim was to investigate the acute responses of the contractile function and the intracellular Ca2+ handling in isolated RV cardiomyocytes after a swimming exercise session. Methods: Ten-week-old female Wistar rats were randomly allocated into two groups: control (C; n = 5) and exercise (Ex; n = 7). It was performed a swimming exercise session for 30 min. with an overload of 4% relative to the body weight attached to the tail. The rats were sacrificed after the exercise session for the analysis of the RV contractile function parameters and the intracellular Ca2+ handling by the isolated cardiomyocyte technique. Results: Body and heart weights, as well as sarcomere length were similar between the groups. Also, it was not observed differences between the groups for RV cardiomyocy...

Arquivos Brasileiros de Cardiologia, 2021

Fundamento: O remodelamento cardíaco patológico se caracteriza por disfunção diastólica e sistóli... more Fundamento: O remodelamento cardíaco patológico se caracteriza por disfunção diastólica e sistólica, levando à insuficiência cardíaca. Neste contexto, o cenário disfuncional do trânsito de cálcio miocárdico (Ca 2+) tem sido pouco estudado. Um modelo experimental de estenose aórtica tem sido extensamente utilizado para aprimorar os conhecimentos sobre os principais mecanismos do remodelamento patológico cardíaco. Objetivo: Entender o processo disfuncional dos principais componentes responsáveis pelo equilíbrio do cálcio miocárdico e sua influência sobre a função cardíaca na insuficiência cardíaca induzida pela estenose aórtica. Métodos: Ratos Wistar de 21 dias de idade foram distribuídos em dois grupos: controle (placebo; n=28) e estenose aórtica (EaO; n=18). A função cardíaca foi analisada com o ecocardiograma, músculo papilar isolado e cardiomiócitos isolados. No ensaio do músculo papilar, SERCA2a e a atividade do canal de Ca 2+ do tipo L foram avaliados. O ensaio de cardiomiócitos isolados avaliou o trânsito de cálcio. A expressão proteica da proteínas do trânsito de cálcio foi analisada com o western blot. Os resultados foram estatisticamente significativos quando p <0,05. Resultados: Os músculos papilares e cardiomiócitos dos corações no grupo EaO demonstraram falhas mecânicas. Os ratos com EaO apresentaram menor tempo de pico do Ca 2+ , menor sensibilidade das miofibrilas do Ca 2+ , prejuízos nos processos de entrada e recaptura de cálcio pelo retículo sarcoplasmático, bem como disfunção no canal de cálcio do tipo L (CCTL). Além disso, os animais com EaO apresentaram maior expressão de SERCA2a, CCTL e trocador de Na + /Ca 2+. Conclusão: Insuficiência cardíaca sistólica e diastólica devido à estenose aórtica supravalvular acarretou comprometimento da entrada de Ca 2+ celular e inibição da recaptura de cálcio pelo retículo sarcoplasmático devido à disfunção no CCTL e SERCA2a, assim como mudanças no trânsito de cálcio e na expressão das principais proteínas responsáveis pela homeostase de Ca 2+ celular. Palavras-chave: Estenose da Valva Aórtica; Insuficiência Cardíaca; Músculos Papilares; Miócitos Cardíacos; Proteínas de Ligação ao Cálcio.

A Insuficiencia Cardiaca (IC) e uma das principais causas de morte na atualidade. Uma maneira de ... more A Insuficiencia Cardiaca (IC) e uma das principais causas de morte na atualidade. Uma maneira de induzir a IC em um rato, para posteriormente estudar medidas que atenuem tal quadro clinico, e simulando uma Estenose Aortica (EAo) por meio da implantacao de um clipe acima de sua valvula aortica. Estudos sugerem que treinamento fisico suaviza os sintomas antecedentes a insuficiencia cardiaca. A fim de comprovar tal hipotese, uma equipe de pesquisadores da Cardiologia Experimental da Faculdade de Medicina de Botucatu vem realizando experimentos (protocolo de aprovacao no CEAU 1138-2015) em que ratos do tipo Wistar sao induzidos a Estenose Aortica e, posteriormente, submetidos a um especifico protocolo de treinamento fisico. Para efeito de comparacao, outros ratos, aqui denominados Sham (sem implantacao do clipe), tambem sao submetidos ao protocolo de treinamento fisico. Portanto, quatro grupos foram formados: EAo (ratos induzidos a estenose aortica); EAoTF (ratos induzidos a estenose ao...

Cellular Physiology and Biochemistry, 2020

This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 I... more This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.

Cellular Physiology and Biochemistry, 2020

This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 I... more This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND). Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.

Frontiers in Physiology, 2019

Experimental studies show that the unsaturated high-fat diet-induced obesity promotes vascular al... more Experimental studies show that the unsaturated high-fat diet-induced obesity promotes vascular alterations characterized by improving the endothelial L-arginine/Nitric Oxide (NO) pathway. Leptin seems to be involved in this process, promoting vasodilation via increasing NO bioavailability. The aim of this study was to test the hypothesis that unsaturated high-fat diet-induced obesity does not generate endothelial dysfunction via increasing the vascular leptin/Akt/eNOS signaling. Thirty-day-old male Wistar rats were randomized into two groups: control (C) and obese (Ob). Group C was fed a standard diet, while group Ob was fed an unsaturated high-fat diet for 27 weeks. Adiposity, hormonal and biochemical parameters, and systolic blood pressure were observed. Concentration response curves were performed for leptin or acetylcholine in the presence or absence of Akt and NOS inhibitor. Our results showed that an unsaturated high-fat diet promoted a greater feed efficiency (FE), elevation of body weight and body fat (BF), and an adiposity index, characterizing a model of obesity. However, comorbidities frequently associated with experimental obesity were not visualized, such as glucose intolerance, dyslipidemia and hypertension. The evaluation of the endothelium-dependent relaxation with acetylcholine showed no differences between the C and Ob rats. After NOS inhibition, the response was completely abolished in the Ob group, but not in the C group. Furthermore, Akt inhibition completely blunted vascular relaxation in the C group, but not in the Ob group, which was more sensitive to leptin-induced vascular relaxation. L-NAME incubation abolished the relaxation in both groups at the same level. Although Akt inhibitor pre-incubation reduced the leptin response, group C was more sensitive to its effect. In conclusion, the high-unsaturated fat diet-induced obesity improved the vascular reactivity to leptin and does not generate endothelial dysfunction, possibly by the increase in the vascular sensitivity to leptin and increasing NO bioavailability. Moreover, our results suggest that the increase in NO production occurs through the increase in NOS activation by leptin and is partially mediated by the Akt pathway.

Revista Brasileira de Medicina do Esporte, 2019

Introduction: Studies indicate that the technical/tactical performance of soccer referees can be ... more Introduction: Studies indicate that the technical/tactical performance of soccer referees can be impacted by physical fitness and state of health. However, although they are routinely invited to take part in matches at national level, it is not yet clearly established whether referees who work in less prominent regions and soccer championships present the same levels of physical, technical and psychological preparation as referees from more important areas, such as the soccer teams of São Paulo and Rio de Janeiro. Within this context, no studies have evaluated the physical conditioning and health parameters of Brazilian Soccer Confederation (CBF) referees who work in Espírito Santo. Objective: To assess the health and physical fitness levels of CBF soccer referees from Espírito Santo. Methods: A cross-sectional study with 16 participants, 6 referees, and 10 assistant referees. The protocol lasted 12 weeks and consisted of the following analyses: general characteristics, body composi...

Pathophysiology : the official journal of the International Society for Pathophysiology, Jan 8, 2018

Cardiac remodeling (CR) is a structural change of the heart due to chronic hemodynamic overload r... more Cardiac remodeling (CR) is a structural change of the heart due to chronic hemodynamic overload related to changes in both myocyte and extracellular matrix (ECM). We investigated that the imbalance of collagen V promotes cardiomyocyte apoptosis that contributes to heart failure and cell death. Aortic stenosis was induced surgically and male Wistar rats were randomized to 18 weeks (Sham 18 w, n = 12; AoS 18 w, n = 12) and severe of heart failure (Sham HF, n = 12; AoS HF, n = 12) groups. Functional and structural echocardiogram, immunohistochemistry for Ki-67, TUNEL assay and Immunofluorescence for collagen were performed. Our main results were: (1) Progressive reduction of cardiac functional capacity due to cardiac remodeling with decreased eject fraction in heart failure; (2) Imbalance of collagen deposition with increased, crowded and irregular collagen I in situ expression; (3) Dysregulation of dynamic control of collagen fibers with exposed epitopes of collagen V; (4) Additional ...

AIDS research and human retroviruses, Jan 6, 2018

HIV infection is characterized by impairment of various epithelial protective mechanisms, factors... more HIV infection is characterized by impairment of various epithelial protective mechanisms, factors that contribute to intestinal barrier damages and result in microbial translocation, leading to increased immune activation and inflammation. However, microbial translocation and inflammatory status in HIV infection has been poorly investigated during pregnancy, period that the women are susceptible to bacterial infections. We aimed to evaluate microbial translocation and the inflammatory status in HIV-infected pregnant women. We studied 30 volunteers, 12 HIV-infected pregnant women (G1), 10 HIV-uninfected pregnant women (G2) and 8 HIV-infected non-pregnant women (G3). The results showed that plasmatic levels of cytokines did not differ among groups. High levels of sCD14 were observed in all groups, with statistical difference in the following comparisons: G1 vs G3 (6727±2030 pg/mL vs 11515±10746 pg/mL; p=0.02) and G2 vs G3 (5256±769 pg/mL vs 11515±10746 pg/mL; p<0.001). Although the...

Physiological reports, 2017

Authors have showed that obesity implicates cardiac dysfunction associated with myocardial L-type... more Authors have showed that obesity implicates cardiac dysfunction associated with myocardial L-type calcium channels (LTCCs) activity impairments, as well as moderate exercise training (MET) seems to be an important therapeutic tool. We tested the hypothesis that MET promotes improvements on LTCCS activity and protein expression at obesity induced by unsaturated high-fat diets, which could represent a protective effects against development of cardiovascular damage. Male Wistar rats were randomized in control (C, n = 40), which received a standard diet and obese (Ob; n = 40), which received high-fat diet. After 20 weeks, the animals were assigned at four groups: control (C; n = 12); control submitted to exercise training (ET; n = 14); obese (Ob; n = 10); and obese submitted to exercise training (ObET; n = 11). ET (5 days/week during 12 weeks) began in the 21th week and consisted of treadmill running that was progressively increased to reach 60 min. Final body weight (FBW), body fat (BF...

ConScientiae Saúde, 2015

Introdução: A obesidade é considerada importante problema de saúde pública e fator de risco para ... more Introdução: A obesidade é considerada importante problema de saúde pública e fator de risco para o desenvolvimento de doenças cardiovasculares. Estudos apontam que o trânsito de cálcio (Ca+2) intracelular e extracelular, mecanismo essencial no acoplamento excitação-contração-relaxamento cardíaco, está envolvido nesse processo patológico. Enquanto o influxo de Ca+2 promove aumento da concentração de Ca+2 livre no citosol na fase de contração, a recaptura e a extrusão do Ca+2 são importantes para a diminuição do Ca+2 intracelular durante o relaxamento. Objetivo: Identificar, baseado na literatura científica, a modulação da disfunção cardíaca pelo trânsito de cálcio em modelos de obesidade genética e dietética. Métodos: A busca de artigos em bases de dados eletrônicas foi realizada com palavras-chaves e seus correspondentes em inglês. Resultados: Inicialmente os artigos que apresentassem uma das palavras-chaves no título foram selecionados. Após processo de triagem, foram identificados...

Medicine & Science in Sports & Exercise, 2015

ConScientiae Saúde, 2015

Introdução: O sistema imunológico apresenta estreita relação com o exercício físico. Todavia, pou... more Introdução: O sistema imunológico apresenta estreita relação com o exercício físico. Todavia, poucos estudos verificaram o perfil imunológico de corredores amadores. Objetivo: Descrever o perfil de marcadores imunológicos de corredores amadores do município de Vitória/ES no período pós-treinamento. Métodos: Foram selecionados 31 corredores pertencentes a dois grupos de corrida, ambos de Vitória/ES. A análise hematológica dos parâmetros imunológicos foi realizada a partir da contagem total de leucócitos por meio de leucograma. Resultados: A contagem de plaquetas apresentou diferença estatística quanto ao gênero, sendo os valores maiores encontrados no gênero feminino em relação ao treinamento contínuo. Além disso, foram encontrados valores menores de neutrófilos no gênero masculino com relação ao treinamento intervalado. Não foi observada diferença estatística entre homens e mulheres nos demais parâmetros analisados. Conclusão: Os resultados deste estudo sugerem que o exercício físic...

Journal of Trainology, 2014

The purpose of this study was to investigate the effects of intensity on rating of perceived exer... more The purpose of this study was to investigate the effects of intensity on rating of perceived exertion (RPE) during multiple sets to volitional failure in bench press exercise. Design and Methods: Thirteen moderately resistance trained men performed 2 experimental sessions in random order: 3 sets to failure in bench press with 1 minute rest at an intensity of 50% and 70% of 1 repetition maximum (1RM). RPE was measured using the OMNI scale after each set. Readiness to Invest in Physical (RTIPE) and Mental (RTIME) Effort were assessed before each set. Results: Repetitions to failure (46.46 ± 9.43 vs 26.62 ± 8.19; P<0.001) and total weight lifted (2044.31 ± 414.5 vs 1650.15 ± 507.83 kg; P<0.001) were greater at 50% when compared to the 70% 1RM condition. There were no differences in RPE, RTIPE, RTIME and heart rate across conditions. Conclusions: These results indicate that when repetitions are done to volitional failure, monitoring RPE is not a useful technique in regulating intensity during resistance training.