Vivek Morya - Academia.edu (original) (raw)
Papers by Vivek Morya
Enzyme and microbial technology, 2018
Synthesis of caffeic acid ester essentially requires an efficient esterification process to produ... more Synthesis of caffeic acid ester essentially requires an efficient esterification process to produce various kinds of medicinally important ester derivatives. In the present study, a comprehensive and comparative analysis of whole-cell catalyzed caffeic acid esters production in ionic liquids (ILs) media was performed. Olive oil induced mycelial mass of halotolerant Aspergillus niger (A.niger) EXF 4321 was freeze dried and used as a catalyst. To ensure maximum solubilization of caffeic acid for highest substrate loading several ILs were screened and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([Emim][TfN]) was found to have the maximum solubility and favoured for enzymatic activity of freeze dried mycelia. The whole-cell catalyzed synthesis of caffeic acid phenethyl ester (CAPE) conditions were optimized and bioconversion up to 84% was achieved at a substrate molar ratio of 1:20 (caffeic acid:2-phenyl ethanol), 30°C for 12h. Results obtained during this study were e...
Process Biochemistry, 2016
Abstract The ionic liquids dubbed as green solvents have been demonstrated to be very effective i... more Abstract The ionic liquids dubbed as green solvents have been demonstrated to be very effective in pre-treatment of various kinds of biomass for better saccharification. Present study describes ionic liquid stability of thermophilic Sporotrichum thermophile xylanase. It was active and stable in 1-ethyl-3-methylimidazolium acetate [EMIM][OAc] up to 50% concentration (v/v). The pre-treatment of wheat straw (18–25% (w/w) xylan) with [EMIM][OAc] followed by IL stable S. thermophile xylanase (containing 12 U/ml xylanase activity and 2 U/ml CMCase activity) treatment led to 281 mg reducing sugars (consisting of mainly xylose and xylo -oligosaccharides) per g wheat straw much higher as compared to non-pre-treated. IL stable enzymes enabled simultaneous pre-treatment, saccharification and the intermediate step of washing of residual IL can be eliminated to make the process cost effective. SEM, XRD and FT-IR confirmed the loss of crystallinity by [EMIM][OAc] pre-treatment followed by hydrolytic bond cleavage and deacylation by xylanase in the subsequent step.
Korean Journal of Chemical Engineering, 2016
Sophorolipids are a kind of glycolipid-based bio-surfactant, and some known yeasts can produce th... more Sophorolipids are a kind of glycolipid-based bio-surfactant, and some known yeasts can produce them by utilizing plant oil at elevated glucose. In spite of a high production yield, sophorolipids have a limited industrial application, due to their narrow range of HLB (hydrophile-lipophile balance) value. These molecules have regained the attention of researchers and industrialists due to their surface properties and versatile applicability in herbal medicine and cosmetics. The bioactivity and surface properties of sophorolipids are mainly governed by chain length and type of the fatty acid. Therefore, the present study was designed to produce and characterize sophorolipids with varying fatty acid chain lengths. Surface-properties like critical micelle concentration of produced sophorolipids were varying from 43 to 62 (dyne/cm). Foamability, dispersion power, and detergence were found to be higher for short chained fatty acids than the longer ones. Cleaning ability of sophorolipid for FLUX coated PCB was found to be better than the chemical surfactants. Biodegradation rates of the sophorolipids were found to be higher than of the linear alkylbenzene sulfonate (LAS) at room temperature as well as 4 o C. These results showed that the properties of sophorolipids can be tuned by varying the chain' s properties of fatty acids, and it may be possible to customize the properties of sophorolipids for specific industrial applications.
Clinical Proteomics, 2012
Aspergillus is a leading causative agent for fungal morbidity and mortality in immuno-compromised... more Aspergillus is a leading causative agent for fungal morbidity and mortality in immuno-compromised patients. To identify a putative target to design or identify new antifungal drug, against Aspergillus is required. In our previous work, we have analyzed the various biochemical pathways, and we found Ketol Acid Reducto-Isomerase (KARI) an enzyme involves in the amino acid biosynthesis, could be a better target. This enzyme was found to be unique by comparing to host proteome through BLASTp analysis. A homology based model of KARI was generated by Swiss model server. The generated model had been validated by PROCHECK and WHAT IF programs. The Zinc library was generated within the limitation of the Lipinski rule of five, for docking study. Based on the dock-score six molecules have been studied for ADME/TOX analysis and subjected for pharmacophore model generation. The Zinc ID of the potential inhibitors is ZINC00720614, ZINC01068126, ZINC0923, ZINC02090678, ZINC00663057 and ZINC02284065 and found to be pharmacologically active agonist and antagonist of KARI. This study is an attempt to Insilco evaluation of the KARI as a drug target and the screened inhibitors could help in the development of the better drug against Aspergillus.
Research Journal of Soil Biology, 2009
Research Trends and Applications, 2013
Journal of Proteomics & Bioinformatics, 2012
Journal of Proteomics & Bioinformatics, 2012
Interdisciplinary Sciences: Computational Life Sciences, 2014
Recombination is one of the keys factor in evolutionary processes, involved in shaping the archit... more Recombination is one of the keys factor in evolutionary processes, involved in shaping the architecture of genomes and consequent phenotype. Understanding the recombination phenomenon especially among viruses will help in disease management. The present study aimed for in-silico analysis of recombination phenomenon among Begomoviruses. Particularly emphasizing on viruses strains reported from India and neighboring countries. A total of 956 virus sequences have been used in this study. The Tomato yellow leaf curl China viruses, namely gi|29825986|; gi|283468151|; gi|190559151| and gi|61652782| were identified with the highest number of recombination event (1273). However, the Mung bean yellow mosaic India virus (gi|66351988|) was found to have 1170 recombination event. The phylogenic analysis among the highly recombinant sequences was carried to get an insight of the evolution among viral sequences in this class of plant viruses. The phylogenetic analysis revealed a pattern in diversity among these virus strains and a split tree analysis showed diversity in the range of 0.049128335 to 10.269852. This in silico analysis may pave way for a greater understanding of recombination phenomenon in Ggeminiviruses and it might be helpful for strategic plant viral disease management.
Enzyme and microbial technology, 2018
Synthesis of caffeic acid ester essentially requires an efficient esterification process to produ... more Synthesis of caffeic acid ester essentially requires an efficient esterification process to produce various kinds of medicinally important ester derivatives. In the present study, a comprehensive and comparative analysis of whole-cell catalyzed caffeic acid esters production in ionic liquids (ILs) media was performed. Olive oil induced mycelial mass of halotolerant Aspergillus niger (A.niger) EXF 4321 was freeze dried and used as a catalyst. To ensure maximum solubilization of caffeic acid for highest substrate loading several ILs were screened and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([Emim][TfN]) was found to have the maximum solubility and favoured for enzymatic activity of freeze dried mycelia. The whole-cell catalyzed synthesis of caffeic acid phenethyl ester (CAPE) conditions were optimized and bioconversion up to 84% was achieved at a substrate molar ratio of 1:20 (caffeic acid:2-phenyl ethanol), 30°C for 12h. Results obtained during this study were e...
Process Biochemistry, 2016
Abstract The ionic liquids dubbed as green solvents have been demonstrated to be very effective i... more Abstract The ionic liquids dubbed as green solvents have been demonstrated to be very effective in pre-treatment of various kinds of biomass for better saccharification. Present study describes ionic liquid stability of thermophilic Sporotrichum thermophile xylanase. It was active and stable in 1-ethyl-3-methylimidazolium acetate [EMIM][OAc] up to 50% concentration (v/v). The pre-treatment of wheat straw (18–25% (w/w) xylan) with [EMIM][OAc] followed by IL stable S. thermophile xylanase (containing 12 U/ml xylanase activity and 2 U/ml CMCase activity) treatment led to 281 mg reducing sugars (consisting of mainly xylose and xylo -oligosaccharides) per g wheat straw much higher as compared to non-pre-treated. IL stable enzymes enabled simultaneous pre-treatment, saccharification and the intermediate step of washing of residual IL can be eliminated to make the process cost effective. SEM, XRD and FT-IR confirmed the loss of crystallinity by [EMIM][OAc] pre-treatment followed by hydrolytic bond cleavage and deacylation by xylanase in the subsequent step.
Korean Journal of Chemical Engineering, 2016
Sophorolipids are a kind of glycolipid-based bio-surfactant, and some known yeasts can produce th... more Sophorolipids are a kind of glycolipid-based bio-surfactant, and some known yeasts can produce them by utilizing plant oil at elevated glucose. In spite of a high production yield, sophorolipids have a limited industrial application, due to their narrow range of HLB (hydrophile-lipophile balance) value. These molecules have regained the attention of researchers and industrialists due to their surface properties and versatile applicability in herbal medicine and cosmetics. The bioactivity and surface properties of sophorolipids are mainly governed by chain length and type of the fatty acid. Therefore, the present study was designed to produce and characterize sophorolipids with varying fatty acid chain lengths. Surface-properties like critical micelle concentration of produced sophorolipids were varying from 43 to 62 (dyne/cm). Foamability, dispersion power, and detergence were found to be higher for short chained fatty acids than the longer ones. Cleaning ability of sophorolipid for FLUX coated PCB was found to be better than the chemical surfactants. Biodegradation rates of the sophorolipids were found to be higher than of the linear alkylbenzene sulfonate (LAS) at room temperature as well as 4 o C. These results showed that the properties of sophorolipids can be tuned by varying the chain' s properties of fatty acids, and it may be possible to customize the properties of sophorolipids for specific industrial applications.
Clinical Proteomics, 2012
Aspergillus is a leading causative agent for fungal morbidity and mortality in immuno-compromised... more Aspergillus is a leading causative agent for fungal morbidity and mortality in immuno-compromised patients. To identify a putative target to design or identify new antifungal drug, against Aspergillus is required. In our previous work, we have analyzed the various biochemical pathways, and we found Ketol Acid Reducto-Isomerase (KARI) an enzyme involves in the amino acid biosynthesis, could be a better target. This enzyme was found to be unique by comparing to host proteome through BLASTp analysis. A homology based model of KARI was generated by Swiss model server. The generated model had been validated by PROCHECK and WHAT IF programs. The Zinc library was generated within the limitation of the Lipinski rule of five, for docking study. Based on the dock-score six molecules have been studied for ADME/TOX analysis and subjected for pharmacophore model generation. The Zinc ID of the potential inhibitors is ZINC00720614, ZINC01068126, ZINC0923, ZINC02090678, ZINC00663057 and ZINC02284065 and found to be pharmacologically active agonist and antagonist of KARI. This study is an attempt to Insilco evaluation of the KARI as a drug target and the screened inhibitors could help in the development of the better drug against Aspergillus.
Research Journal of Soil Biology, 2009
Research Trends and Applications, 2013
Journal of Proteomics & Bioinformatics, 2012
Journal of Proteomics & Bioinformatics, 2012
Interdisciplinary Sciences: Computational Life Sciences, 2014
Recombination is one of the keys factor in evolutionary processes, involved in shaping the archit... more Recombination is one of the keys factor in evolutionary processes, involved in shaping the architecture of genomes and consequent phenotype. Understanding the recombination phenomenon especially among viruses will help in disease management. The present study aimed for in-silico analysis of recombination phenomenon among Begomoviruses. Particularly emphasizing on viruses strains reported from India and neighboring countries. A total of 956 virus sequences have been used in this study. The Tomato yellow leaf curl China viruses, namely gi|29825986|; gi|283468151|; gi|190559151| and gi|61652782| were identified with the highest number of recombination event (1273). However, the Mung bean yellow mosaic India virus (gi|66351988|) was found to have 1170 recombination event. The phylogenic analysis among the highly recombinant sequences was carried to get an insight of the evolution among viral sequences in this class of plant viruses. The phylogenetic analysis revealed a pattern in diversity among these virus strains and a split tree analysis showed diversity in the range of 0.049128335 to 10.269852. This in silico analysis may pave way for a greater understanding of recombination phenomenon in Ggeminiviruses and it might be helpful for strategic plant viral disease management.