Vincent Pizziconi - Academia.edu (original) (raw)

Papers by Vincent Pizziconi

Research paper thumbnail of Remote Scanning Probe Microscopy and its Uses in Distancelearning and Educational Outreach

Microscopy and Microanalysis, 2001

: Tele-microscopy has become a very active area of research and development in the 1990s where th... more : Tele-microscopy has become a very active area of research and development in the 1990s where there was a desire to give researchers better access to expensive and specialized microscopes, breaking geographical and time barriers. Rapid advances in telecommunication, computers, and microscopy technologies make possible the establishment of the World Wide Web and the realization of the goal of developing a “laboratory without walls”. This provides unprecedented opportunities for researchers and educators alike to gain access to shared instrumental and educational resources.In this presentation, a description of the Interactive Nano-Visualization for Science and Engineering Education (IN-VSEE) project will be given. The primary goals of IN-VSEE are to (i) convey the excitement of nanoscience and nanotechnology to promote studentmotivated learning and pursuit of science and engineering careers, (ii) teach fundamental interdisciplinary concepts in science and engineering using a visual format to help students learn and integrate information more effectively, (iii) provide students with the capability to routinely explore materials in three dimensions with resolutions at the nanoscale and even down to the atomic scale, and (iv) demonstrate the feasibility of remote operation of research-grade laboratory instrumentation for development into a powerful educational and collaborative tool.

Research paper thumbnail of Abstract TP288: Selective Brain Hypothermia Induced by Thermoelectric Cooling

Research paper thumbnail of The application of ion beam analysis to calcium phosphate‐based biomaterials

Journal of Biomedical Materials Research, Feb 1, 1996

Ion beam technology may be applied in a straightforward fashion to the analysis and modification ... more Ion beam technology may be applied in a straightforward fashion to the analysis and modification of biomaterials. For analytical purposes, characterization using megaelectron-volt He2+ ions provides a standardless, nondestructive means for accurately quantifying the composition of material surfaces and the thickness of thin films. In this study, three complementary ion backscattering techniques were utilized to characterize hydroxyapatite (HA) films: Rutherford backscattering spectrometry (RBS) can determine composition and amounts of elements heavier than He; forward recoil elastic spectrometry (FRES) can determine hydrogen content; resonance-enhanced RBS can quantify small amounts of light elements, e.g. carbon, by choosing a particular incident beam energy resulting in excitation of the light element nucleus. At this resonance energy, the scattering cross section greatly increases, improving elemental sensitivity. Sol-gel chemistry was used to synthesize HA films by spin coating and annealing in a rapid thermal processor. Using these techniques, the chemical composition of unfired films was Ca1.63O5.4H1.8C0.24P with a thickness of 3.01 x 10(18) atoms/cm2 and after firing at 800 degrees C as Ca1.66O4.0H0.26C0.09P with a thickness of 2.11 x 10(18) atoms/cm2. This compares favorably to stoichiometric HA, which has a composition of Ca1.67O4.33H0.33P.

Research paper thumbnail of Interactive nano-visualization in science and engineering education: Conforming technology to transform education: The IN-VSEE project

Abstract The Interactive Nano-Visualization in Science and Engineering Education (IN-VSEE) projec... more Abstract The Interactive Nano-Visualization in Science and Engineering Education (IN-VSEE) project combines advances in telecommunications, instructional technologies, and science and engineering research to provide the first real-time interface for remote ...

Research paper thumbnail of Remote Scanning Probe Microscopy and its Uses in Distancelearning and Educational Outreach

Microscopy and Microanalysis, Aug 1, 2001

: Tele-microscopy has become a very active area of research and development in the 1990s where th... more : Tele-microscopy has become a very active area of research and development in the 1990s where there was a desire to give researchers better access to expensive and specialized microscopes, breaking geographical and time barriers. Rapid advances in telecommunication, computers, and microscopy technologies make possible the establishment of the World Wide Web and the realization of the goal of developing a “laboratory without walls”. This provides unprecedented opportunities for researchers and educators alike to gain access to shared instrumental and educational resources.In this presentation, a description of the Interactive Nano-Visualization for Science and Engineering Education (IN-VSEE) project will be given. The primary goals of IN-VSEE are to (i) convey the excitement of nanoscience and nanotechnology to promote studentmotivated learning and pursuit of science and engineering careers, (ii) teach fundamental interdisciplinary concepts in science and engineering using a visual format to help students learn and integrate information more effectively, (iii) provide students with the capability to routinely explore materials in three dimensions with resolutions at the nanoscale and even down to the atomic scale, and (iv) demonstrate the feasibility of remote operation of research-grade laboratory instrumentation for development into a powerful educational and collaborative tool.

Research paper thumbnail of Why Does a Light Bulb Burn Out?

MRS Proceedings, 2000

ABSTRACTThe online educational module “Why Does a Light Bulb Burn Out?” is an inquiry-based intro... more ABSTRACTThe online educational module “Why Does a Light Bulb Burn Out?” is an inquiry-based introduction to the concepts of materials and material properties through the interactive exploration of the life of the incandescent light bulb that students use every day. The module offers an exploration of the history of the light bulb, its components, and important filament properties. Students discover the relationship between temperature and incandescence, along with electrical power and resistance through interactive Java applets. Then students “invent” their own filaments through virtual temperature, performance and longevity tests of a variety of candidate materials. Next, students follow the filament aging process using scanning-electron/atomic-force microscopy images. The module culminates in students designing their own materials experiments using SPM Live! online at http://invsee.asu.edu. Student evaluations indicate students both enjoy and learn effectively using the module.

Research paper thumbnail of A cell-based immunobiosensor with engineered molecular recognition— Part III: engineering molecular recognition

Biosensors and Bioelectronics, 1997

A novel bioelectronic sensor is described in which living immune cells are transformed into uniqu... more A novel bioelectronic sensor is described in which living immune cells are transformed into unique biotransducer couples by engineering their molecular recognition for preselected antigens of clinical interest. This 'hybrid' biosensor, constructed with mast cells interfaced to a microfabricated thermoelectric device with the use of biomolecular linkages, is capable of detecting antigens in real time by transducing minute heat changes arising from antigeninduced mast cell activation processes. The thermoelectric approach was selected based upon preliminary bioenergetic calculations which indicated that metabolic changes arising from mast cell antigen recognition result in a significant increase in exothermic heat relative to basal metabolic conditions. Experimental studies confirmed that mast cell activation and degranulation can be discriminated thermally from basal metabolic activity. Results obtained from microcalorimetry experiments using cultured mast cells (MC/9 mucosal-like mast cell line), and harvested mast cells (rat peritoneal mast cells) indicated that detectable increases in heat output (-3 _-+ 0.5 pW/cell, mean peak output) immediately followed cell activation. The construction of a miniature hybrid immunobiosensor device was made possible by bioelectronic coupling achieved with the use of cellular adhesive proteins that immobilized non-adherent (MC/9) cells as well as adherent (RBL-2H3 rat basophilic leukemia) cells to the thermopile. Results from preliminary tests conducted on a hybrid biosensor prototype validated the design feasibility of a miniature, living cell immunodiagnostic biosensor. Such cellbased hybrid biosensor approaches may greatly extend the capability for selective, rapid, on-site, antigen detection for a wide range of clinically relevant antigens and offer new approaches to in vitro diagnostics.

Research paper thumbnail of Augmented hemoperfusion for hyperbilirubinemia

PubMed, Jul 1, 1988

Exchange transfusions and phototherapy are used to treat hyperbilirubinemia, each method having i... more Exchange transfusions and phototherapy are used to treat hyperbilirubinemia, each method having its own disadvantages. Hemoperfusion with coated activated charcoal (CAC) produces low removal rates due to the strong binding of bilirubin to albumin. Biocompatible chemical agents were investigated to selectively unbind the bilirubin into solution just prior to CAC exposure. In batch mock solution tests, the addition of sodium benzoate resulted in a 69% equilibrium bilirubin removal at 50 mM and 96% removal at 100 mM. During flow tests, adsorptive removal of sodium benzoate was so rapid that the CAC had to be pretreated with sodium benzoate solutions. In the absence of sodium benzoate, the outlet bilirubin was 50% of the inlet concentration at the passage of one void volume, with a rapid increase to 70% (30% removal). With sodium benzoate at 100 mM, the same 50% outlet/inlet percent was observed at one void volume throughput, but the outlet concentration fell to 6% of the inlet at 12 column void volumes (94% removal). Similar experiments with bovine plasma/blood and human plasma resulted in an average increase in bilirubin removal of 25% for the bovine and human plasma and 35% for the bovine blood at 100 mM benzoate. A significant decrease in platelet aggregation was measured with the addition of sodium benzoate, which makes this augmented hemoperfusion removal of bilirubin clinically attractive.

Research paper thumbnail of Correlation of pulsed flow vortex shedding

PubMed, 1975

Flow visualization experiments were performed leading to the development of a correlation for the... more Flow visualization experiments were performed leading to the development of a correlation for the vortex shedding from indentations perpendicular to the main stream flow and caused by the addition of a pulsed flow component. The peak pulse flow rate required for vortex shedding greatly exceeds the condition for flow reversal in the channel when the steady flow rate is low while the vortex shedding occurs close to the flow reversal condition when the steady flow rate is very high. The empirical correlation should be useful in assessing the practicality of utilizing this technique to augment mass transport in membrane separation processes. Vortex shedding provides substantial transport augmentation in the ultrafiltration of a flowing plasma stream, and recommendations for further study are outlined to improve our understanding of this phenomena.

Research paper thumbnail of Transfer of chemical species through a protein gel

PubMed, 1971

Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for AS... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for ASAIO Journal. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed ...

Research paper thumbnail of A cell-based immunobiosensor with engineered molecular recognition—Part I: design feasibility

Biosensors and Bioelectronics, 1997

A novel bioelectronic sensor is described in which living immune cells are transformed into uniqu... more A novel bioelectronic sensor is described in which living immune cells are transformed into unique biotransducer couples by engineering their molecular recognition for preselected antigens of clinical interest. This 'hybrid' biosensor, constructed with mast cells interfaced to a microfabricated thermoelectric device with the use of biomolecular linkages, is capable of detecting antigens in real time by transducing minute heat changes arising from antigeninduced mast cell activation processes. The thermoelectric approach was selected based upon preliminary bioenergetic calculations which indicated that metabolic changes arising from mast cell antigen recognition result in a significant increase in exothermic heat relative to basal metabolic conditions. Experimental studies confirmed that mast cell activation and degranulation can be discriminated thermally from basal metabolic activity. Results obtained from microcalorimetry experiments using cultured mast cells (MC/9 mucosal-like mast cell line), and harvested mast cells (rat peritoneal mast cells) indicated that detectable increases in heat output (-3 _-+ 0.5 pW/cell, mean peak output) immediately followed cell activation. The construction of a miniature hybrid immunobiosensor device was made possible by bioelectronic coupling achieved with the use of cellular adhesive proteins that immobilized non-adherent (MC/9) cells as well as adherent (RBL-2H3 rat basophilic leukemia) cells to the thermopile. Results from preliminary tests conducted on a hybrid biosensor prototype validated the design feasibility of a miniature, living cell immunodiagnostic biosensor. Such cellbased hybrid biosensor approaches may greatly extend the capability for selective, rapid, on-site, antigen detection for a wide range of clinically relevant antigens and offer new approaches to in vitro diagnostics.

Research paper thumbnail of Ultrafiltration of molecules through deposited protein layers

PubMed, 1975

A naturally deposited protein layer is shown to be highly compressible as witnessed by its resist... more A naturally deposited protein layer is shown to be highly compressible as witnessed by its resistance to the passage of both water and dissolved molecules. This protein layer can easily be much more important than the underlying membrane. Hydrodynamic methods can be employed to control this protein layer, even to the extreme of its extinction in a continuous flow system. The rejection properties are shown to be a function of both the tracer molecular weight and type. Some of the physical property variations are reviewed for plasma amd serum as a function of protein concentration.

Research paper thumbnail of An efficient method for removing bilirubin

ASAIO transactions / American Society for Artificial Internal Organs

Bilirubin is tightly bound to albumin, making hemoperfusion an ineffective treatment for hyperbil... more Bilirubin is tightly bound to albumin, making hemoperfusion an ineffective treatment for hyperbilirubinemia. By adding a safe unbinding agent to the blood (solutizer), which itself is adsorbed, hemoperfusion can become efficient and practical. Canines were made hyperbilirubinemic with an intravenous infusion of a 5 mg/ml solution (with Na2CO3) for 1 hour. Peak concentrations of 14-22 mg/dl were reached in adult dogs (25-35 kg). Hemoperfusion was then initiated with or without (control) the solutizer (sodium benzoate). The bilirubin unbinding effect of sodium benzoate was rapid and effective. Because of the simultaneous adsorption of sodium benzoate, a small activated carbon section that was presaturated with the solutizer was located proximal to the main hemoperfusion column, in addition to continuous infusion to reach 20 mM in the blood. Comparison of the normalized bilirubin concentration for benzoate augmented hemoperfusion with the average for control dogs, shows that benzoate r...

Research paper thumbnail of Molecular separation as an artificial kidney technique

Transactions - American Society for Artificial Internal Organs, 1970

Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for AS... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for ASAIO Journal. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed ...

Research paper thumbnail of High-efficiency hemofiltration

ASAIO transactions / American Society for Artificial Internal Organs

Research paper thumbnail of A new technique for characterization of pore structures in materials—application to the study of hydroxyapatite thin films

Materials Letters, 1998

A new technique for characterizing the pore structures in thin films has been developed. The tech... more A new technique for characterizing the pore structures in thin films has been developed. The technique is rapid compared to other measurement techniques, and it also provides a depth distribution of the open porosity of the film. Using this technique, the pore structure of sol-gel derived hydroxyapatite thin films was determined.

Research paper thumbnail of ¿ Por qué se funde un foco?

Research paper thumbnail of Barrow Innovation Center: A 5-Year Update and Future Direction

Research paper thumbnail of Characterization of Chlorobium tepidum Chlorosomes: A Calculation

All in-text references underlined in blue are linked to publications on ResearchGate, letting you... more All in-text references underlined in blue are linked to publications on ResearchGate, letting you access and read them immediately.

Research paper thumbnail of Engaging Middle School Students With Engineering Education, Curricular Integration And Societal Relevance

2010 Annual Conference & Exposition Proceedings

is an Associate Professor in the Harrington Bioengineering Program within the School of Biologica... more is an Associate Professor in the Harrington Bioengineering Program within the School of Biological and Health Systems Engineering in the Fulton School of Engineering at ASU. His teaching responsibilities are in the areas of engineering design, biomaterials, nanobiotechnology, and complex adaptive systems. He is the Director of the Bioengineering Design Center, a state-of-the-art design studio and prototype complex funded by the Whitaker Foundation, and the Biomedical Engineering Capstone Design Education Program and co-directs the 'Empowering Malawians with Disabilities' project that is aimed at the design and development of rehabilitative engineered devices to assist the disabled and under served in Malawi, Africa. His education interests are in innovative education in engineering and engineering outreach that include K-12 outreach programs, interdisciplinary undergraduate (REU) and graduate training and outreach programs (NSF IGERT & GK-12), women in science and engineering programs (WISE), and innovative nanoscience and engineering asynchronous distance learning initiatives. He is currently working on an NSF supported IEECI grant developing materials for middle school outreach based on service learning using engineering design principles. His current research interests are focused on the development of micro and nano biodevices and technologies for personalized medicine, bioinspired nanoscale engineering strategies for bioresponsive and biomimetic hybrid materials and biohybrid devices for diagnostic and therapeutic medical device applications. He also serves on various scientific review panels and advisory panels, as well as, a consultant to the medical device and diagnostic industry.

Research paper thumbnail of Remote Scanning Probe Microscopy and its Uses in Distancelearning and Educational Outreach

Microscopy and Microanalysis, 2001

: Tele-microscopy has become a very active area of research and development in the 1990s where th... more : Tele-microscopy has become a very active area of research and development in the 1990s where there was a desire to give researchers better access to expensive and specialized microscopes, breaking geographical and time barriers. Rapid advances in telecommunication, computers, and microscopy technologies make possible the establishment of the World Wide Web and the realization of the goal of developing a “laboratory without walls”. This provides unprecedented opportunities for researchers and educators alike to gain access to shared instrumental and educational resources.In this presentation, a description of the Interactive Nano-Visualization for Science and Engineering Education (IN-VSEE) project will be given. The primary goals of IN-VSEE are to (i) convey the excitement of nanoscience and nanotechnology to promote studentmotivated learning and pursuit of science and engineering careers, (ii) teach fundamental interdisciplinary concepts in science and engineering using a visual format to help students learn and integrate information more effectively, (iii) provide students with the capability to routinely explore materials in three dimensions with resolutions at the nanoscale and even down to the atomic scale, and (iv) demonstrate the feasibility of remote operation of research-grade laboratory instrumentation for development into a powerful educational and collaborative tool.

Research paper thumbnail of Abstract TP288: Selective Brain Hypothermia Induced by Thermoelectric Cooling

Research paper thumbnail of The application of ion beam analysis to calcium phosphate‐based biomaterials

Journal of Biomedical Materials Research, Feb 1, 1996

Ion beam technology may be applied in a straightforward fashion to the analysis and modification ... more Ion beam technology may be applied in a straightforward fashion to the analysis and modification of biomaterials. For analytical purposes, characterization using megaelectron-volt He2+ ions provides a standardless, nondestructive means for accurately quantifying the composition of material surfaces and the thickness of thin films. In this study, three complementary ion backscattering techniques were utilized to characterize hydroxyapatite (HA) films: Rutherford backscattering spectrometry (RBS) can determine composition and amounts of elements heavier than He; forward recoil elastic spectrometry (FRES) can determine hydrogen content; resonance-enhanced RBS can quantify small amounts of light elements, e.g. carbon, by choosing a particular incident beam energy resulting in excitation of the light element nucleus. At this resonance energy, the scattering cross section greatly increases, improving elemental sensitivity. Sol-gel chemistry was used to synthesize HA films by spin coating and annealing in a rapid thermal processor. Using these techniques, the chemical composition of unfired films was Ca1.63O5.4H1.8C0.24P with a thickness of 3.01 x 10(18) atoms/cm2 and after firing at 800 degrees C as Ca1.66O4.0H0.26C0.09P with a thickness of 2.11 x 10(18) atoms/cm2. This compares favorably to stoichiometric HA, which has a composition of Ca1.67O4.33H0.33P.

Research paper thumbnail of Interactive nano-visualization in science and engineering education: Conforming technology to transform education: The IN-VSEE project

Abstract The Interactive Nano-Visualization in Science and Engineering Education (IN-VSEE) projec... more Abstract The Interactive Nano-Visualization in Science and Engineering Education (IN-VSEE) project combines advances in telecommunications, instructional technologies, and science and engineering research to provide the first real-time interface for remote ...

Research paper thumbnail of Remote Scanning Probe Microscopy and its Uses in Distancelearning and Educational Outreach

Microscopy and Microanalysis, Aug 1, 2001

: Tele-microscopy has become a very active area of research and development in the 1990s where th... more : Tele-microscopy has become a very active area of research and development in the 1990s where there was a desire to give researchers better access to expensive and specialized microscopes, breaking geographical and time barriers. Rapid advances in telecommunication, computers, and microscopy technologies make possible the establishment of the World Wide Web and the realization of the goal of developing a “laboratory without walls”. This provides unprecedented opportunities for researchers and educators alike to gain access to shared instrumental and educational resources.In this presentation, a description of the Interactive Nano-Visualization for Science and Engineering Education (IN-VSEE) project will be given. The primary goals of IN-VSEE are to (i) convey the excitement of nanoscience and nanotechnology to promote studentmotivated learning and pursuit of science and engineering careers, (ii) teach fundamental interdisciplinary concepts in science and engineering using a visual format to help students learn and integrate information more effectively, (iii) provide students with the capability to routinely explore materials in three dimensions with resolutions at the nanoscale and even down to the atomic scale, and (iv) demonstrate the feasibility of remote operation of research-grade laboratory instrumentation for development into a powerful educational and collaborative tool.

Research paper thumbnail of Why Does a Light Bulb Burn Out?

MRS Proceedings, 2000

ABSTRACTThe online educational module “Why Does a Light Bulb Burn Out?” is an inquiry-based intro... more ABSTRACTThe online educational module “Why Does a Light Bulb Burn Out?” is an inquiry-based introduction to the concepts of materials and material properties through the interactive exploration of the life of the incandescent light bulb that students use every day. The module offers an exploration of the history of the light bulb, its components, and important filament properties. Students discover the relationship between temperature and incandescence, along with electrical power and resistance through interactive Java applets. Then students “invent” their own filaments through virtual temperature, performance and longevity tests of a variety of candidate materials. Next, students follow the filament aging process using scanning-electron/atomic-force microscopy images. The module culminates in students designing their own materials experiments using SPM Live! online at http://invsee.asu.edu. Student evaluations indicate students both enjoy and learn effectively using the module.

Research paper thumbnail of A cell-based immunobiosensor with engineered molecular recognition— Part III: engineering molecular recognition

Biosensors and Bioelectronics, 1997

A novel bioelectronic sensor is described in which living immune cells are transformed into uniqu... more A novel bioelectronic sensor is described in which living immune cells are transformed into unique biotransducer couples by engineering their molecular recognition for preselected antigens of clinical interest. This 'hybrid' biosensor, constructed with mast cells interfaced to a microfabricated thermoelectric device with the use of biomolecular linkages, is capable of detecting antigens in real time by transducing minute heat changes arising from antigeninduced mast cell activation processes. The thermoelectric approach was selected based upon preliminary bioenergetic calculations which indicated that metabolic changes arising from mast cell antigen recognition result in a significant increase in exothermic heat relative to basal metabolic conditions. Experimental studies confirmed that mast cell activation and degranulation can be discriminated thermally from basal metabolic activity. Results obtained from microcalorimetry experiments using cultured mast cells (MC/9 mucosal-like mast cell line), and harvested mast cells (rat peritoneal mast cells) indicated that detectable increases in heat output (-3 _-+ 0.5 pW/cell, mean peak output) immediately followed cell activation. The construction of a miniature hybrid immunobiosensor device was made possible by bioelectronic coupling achieved with the use of cellular adhesive proteins that immobilized non-adherent (MC/9) cells as well as adherent (RBL-2H3 rat basophilic leukemia) cells to the thermopile. Results from preliminary tests conducted on a hybrid biosensor prototype validated the design feasibility of a miniature, living cell immunodiagnostic biosensor. Such cellbased hybrid biosensor approaches may greatly extend the capability for selective, rapid, on-site, antigen detection for a wide range of clinically relevant antigens and offer new approaches to in vitro diagnostics.

Research paper thumbnail of Augmented hemoperfusion for hyperbilirubinemia

PubMed, Jul 1, 1988

Exchange transfusions and phototherapy are used to treat hyperbilirubinemia, each method having i... more Exchange transfusions and phototherapy are used to treat hyperbilirubinemia, each method having its own disadvantages. Hemoperfusion with coated activated charcoal (CAC) produces low removal rates due to the strong binding of bilirubin to albumin. Biocompatible chemical agents were investigated to selectively unbind the bilirubin into solution just prior to CAC exposure. In batch mock solution tests, the addition of sodium benzoate resulted in a 69% equilibrium bilirubin removal at 50 mM and 96% removal at 100 mM. During flow tests, adsorptive removal of sodium benzoate was so rapid that the CAC had to be pretreated with sodium benzoate solutions. In the absence of sodium benzoate, the outlet bilirubin was 50% of the inlet concentration at the passage of one void volume, with a rapid increase to 70% (30% removal). With sodium benzoate at 100 mM, the same 50% outlet/inlet percent was observed at one void volume throughput, but the outlet concentration fell to 6% of the inlet at 12 column void volumes (94% removal). Similar experiments with bovine plasma/blood and human plasma resulted in an average increase in bilirubin removal of 25% for the bovine and human plasma and 35% for the bovine blood at 100 mM benzoate. A significant decrease in platelet aggregation was measured with the addition of sodium benzoate, which makes this augmented hemoperfusion removal of bilirubin clinically attractive.

Research paper thumbnail of Correlation of pulsed flow vortex shedding

PubMed, 1975

Flow visualization experiments were performed leading to the development of a correlation for the... more Flow visualization experiments were performed leading to the development of a correlation for the vortex shedding from indentations perpendicular to the main stream flow and caused by the addition of a pulsed flow component. The peak pulse flow rate required for vortex shedding greatly exceeds the condition for flow reversal in the channel when the steady flow rate is low while the vortex shedding occurs close to the flow reversal condition when the steady flow rate is very high. The empirical correlation should be useful in assessing the practicality of utilizing this technique to augment mass transport in membrane separation processes. Vortex shedding provides substantial transport augmentation in the ultrafiltration of a flowing plasma stream, and recommendations for further study are outlined to improve our understanding of this phenomena.

Research paper thumbnail of Transfer of chemical species through a protein gel

PubMed, 1971

Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for AS... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for ASAIO Journal. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed ...

Research paper thumbnail of A cell-based immunobiosensor with engineered molecular recognition—Part I: design feasibility

Biosensors and Bioelectronics, 1997

A novel bioelectronic sensor is described in which living immune cells are transformed into uniqu... more A novel bioelectronic sensor is described in which living immune cells are transformed into unique biotransducer couples by engineering their molecular recognition for preselected antigens of clinical interest. This 'hybrid' biosensor, constructed with mast cells interfaced to a microfabricated thermoelectric device with the use of biomolecular linkages, is capable of detecting antigens in real time by transducing minute heat changes arising from antigeninduced mast cell activation processes. The thermoelectric approach was selected based upon preliminary bioenergetic calculations which indicated that metabolic changes arising from mast cell antigen recognition result in a significant increase in exothermic heat relative to basal metabolic conditions. Experimental studies confirmed that mast cell activation and degranulation can be discriminated thermally from basal metabolic activity. Results obtained from microcalorimetry experiments using cultured mast cells (MC/9 mucosal-like mast cell line), and harvested mast cells (rat peritoneal mast cells) indicated that detectable increases in heat output (-3 _-+ 0.5 pW/cell, mean peak output) immediately followed cell activation. The construction of a miniature hybrid immunobiosensor device was made possible by bioelectronic coupling achieved with the use of cellular adhesive proteins that immobilized non-adherent (MC/9) cells as well as adherent (RBL-2H3 rat basophilic leukemia) cells to the thermopile. Results from preliminary tests conducted on a hybrid biosensor prototype validated the design feasibility of a miniature, living cell immunodiagnostic biosensor. Such cellbased hybrid biosensor approaches may greatly extend the capability for selective, rapid, on-site, antigen detection for a wide range of clinically relevant antigens and offer new approaches to in vitro diagnostics.

Research paper thumbnail of Ultrafiltration of molecules through deposited protein layers

PubMed, 1975

A naturally deposited protein layer is shown to be highly compressible as witnessed by its resist... more A naturally deposited protein layer is shown to be highly compressible as witnessed by its resistance to the passage of both water and dissolved molecules. This protein layer can easily be much more important than the underlying membrane. Hydrodynamic methods can be employed to control this protein layer, even to the extreme of its extinction in a continuous flow system. The rejection properties are shown to be a function of both the tracer molecular weight and type. Some of the physical property variations are reviewed for plasma amd serum as a function of protein concentration.

Research paper thumbnail of An efficient method for removing bilirubin

ASAIO transactions / American Society for Artificial Internal Organs

Bilirubin is tightly bound to albumin, making hemoperfusion an ineffective treatment for hyperbil... more Bilirubin is tightly bound to albumin, making hemoperfusion an ineffective treatment for hyperbilirubinemia. By adding a safe unbinding agent to the blood (solutizer), which itself is adsorbed, hemoperfusion can become efficient and practical. Canines were made hyperbilirubinemic with an intravenous infusion of a 5 mg/ml solution (with Na2CO3) for 1 hour. Peak concentrations of 14-22 mg/dl were reached in adult dogs (25-35 kg). Hemoperfusion was then initiated with or without (control) the solutizer (sodium benzoate). The bilirubin unbinding effect of sodium benzoate was rapid and effective. Because of the simultaneous adsorption of sodium benzoate, a small activated carbon section that was presaturated with the solutizer was located proximal to the main hemoperfusion column, in addition to continuous infusion to reach 20 mM in the blood. Comparison of the normalized bilirubin concentration for benzoate augmented hemoperfusion with the average for control dogs, shows that benzoate r...

Research paper thumbnail of Molecular separation as an artificial kidney technique

Transactions - American Society for Artificial Internal Organs, 1970

Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for AS... more Advertisement. Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for ASAIO Journal. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed ...

Research paper thumbnail of High-efficiency hemofiltration

ASAIO transactions / American Society for Artificial Internal Organs

Research paper thumbnail of A new technique for characterization of pore structures in materials—application to the study of hydroxyapatite thin films

Materials Letters, 1998

A new technique for characterizing the pore structures in thin films has been developed. The tech... more A new technique for characterizing the pore structures in thin films has been developed. The technique is rapid compared to other measurement techniques, and it also provides a depth distribution of the open porosity of the film. Using this technique, the pore structure of sol-gel derived hydroxyapatite thin films was determined.

Research paper thumbnail of ¿ Por qué se funde un foco?

Research paper thumbnail of Barrow Innovation Center: A 5-Year Update and Future Direction

Research paper thumbnail of Characterization of Chlorobium tepidum Chlorosomes: A Calculation

All in-text references underlined in blue are linked to publications on ResearchGate, letting you... more All in-text references underlined in blue are linked to publications on ResearchGate, letting you access and read them immediately.

Research paper thumbnail of Engaging Middle School Students With Engineering Education, Curricular Integration And Societal Relevance

2010 Annual Conference & Exposition Proceedings

is an Associate Professor in the Harrington Bioengineering Program within the School of Biologica... more is an Associate Professor in the Harrington Bioengineering Program within the School of Biological and Health Systems Engineering in the Fulton School of Engineering at ASU. His teaching responsibilities are in the areas of engineering design, biomaterials, nanobiotechnology, and complex adaptive systems. He is the Director of the Bioengineering Design Center, a state-of-the-art design studio and prototype complex funded by the Whitaker Foundation, and the Biomedical Engineering Capstone Design Education Program and co-directs the 'Empowering Malawians with Disabilities' project that is aimed at the design and development of rehabilitative engineered devices to assist the disabled and under served in Malawi, Africa. His education interests are in innovative education in engineering and engineering outreach that include K-12 outreach programs, interdisciplinary undergraduate (REU) and graduate training and outreach programs (NSF IGERT & GK-12), women in science and engineering programs (WISE), and innovative nanoscience and engineering asynchronous distance learning initiatives. He is currently working on an NSF supported IEECI grant developing materials for middle school outreach based on service learning using engineering design principles. His current research interests are focused on the development of micro and nano biodevices and technologies for personalized medicine, bioinspired nanoscale engineering strategies for bioresponsive and biomimetic hybrid materials and biohybrid devices for diagnostic and therapeutic medical device applications. He also serves on various scientific review panels and advisory panels, as well as, a consultant to the medical device and diagnostic industry.