V. Savino - Academia.edu (original) (raw)

Papers by V. Savino

Journal of Phytopathology, 1990

ABSTRACT Abstract Grapevine phloem-limited isometric virus (GPLIV) is the name proposed for a non... more ABSTRACT Abstract Grapevine phloem-limited isometric virus (GPLIV) is the name proposed for a non mechanically-transmissible virus found in Italian and Tunisian grapevines. In density gradient centrifugation purified virus preparations sedimented as two components: T, made up of empty protein shells, and B, composed of intact nucleoprotein particles. B particles had a buoyant density of 1.45 g/cm3 at equilibrium in CsCl and contained 35% RNA consisting of a single molecule with an apparent size of 7.4 kb. The coat protein consisted of a single species with a mol.wt of 28,000 daltons. Purified virus preparations did not infect herbaceous hosts by manual inoculation. A specific antiserum with a titre of 1: 64 raised in rabbits, was used for identification of, GPLIV in field-grown Tunisian grapevines and in leafroll-affected Italian vines before and after heat treatment. Although heat treatment eliminated the virus from the majority of the plants, leafroll symptoms persisted in several GPLIV-free vines, indicating that there is no clear-cut relationship between GPLIV and this disease.ZusammenfassungEinige Eigenschaften eJnes im Phloem begrenzten, nicht mechanisch iibertragbaren WeinstockvirusDer Name grapevine phloem-limited isometric virus [GPLIV) wird für ein nicht mechanisch übertragbares, in Italien und Tunesien vorkommendes Virus vorgeschlagen, Nach Dichtegradiemenzentnfugation konnten zwei Komponenten aus gereinigten Virusprparaten festgestellt werden: T, die aus leeren Proteinhüllen bestand, und B, die ganze Nucieoproteinpartikein enthielt. Die B-Komponenten hatten eine schwimmende Dichte von 1,45 g/cm3 im Gleichgewicht mit CsCl und emhielten 35 % RNS, die aus eincm einzelnen Molekül mit einer scheitibaren Größe von 7,4 kb bestand. Das Hüllprotein setzte sich aus einer Art mit einem Mokkulargewicht von 28 000 Dalton zusammen. Gereinigte Virusprparaie waren nicht in der Lage, Wirtspflanzen nach einer kiinsthchen Inokulation zu lnfizieren. Ein aus Kaninchen gewonnenes, spezifisches Antiserum, mit einem Titer von 1: 64, wurde bei der Feststellung vom CPLIV in Weinstöcken aus Feldern in Tunesien und in Reben in Italien. die geroiite Blatter aufwiesen, vor und nach einer Hitzebehandlung angewandt. Obwohl Nach einer Hitzebehandlung das Virus in fast alien Pflanzen nicht mehr nachzuweisen war, wurden die gerollten Blttersymptome bei einigen GPLIV-freien Reben nicht aufgehoben. Dies deutet darauf hin, daß es keinen eindeutigen Zusammenhang zwischen dem GPLIV und dieser Krankheit gibt.

European Journal of Plant Pathology, 1996

A virus was recovered by sap transmission from plants of several citrus species exhibiting or not... more A virus was recovered by sap transmission from plants of several citrus species exhibiting or not symptoms of chlorotic dwarf (CCD), a disease recently reported from Eastern Mediterranean Turkey. The virus was identified as an isolate of olive latent virus 1 (OLV-1), originally described as a possible sobemovirus. The citrus isolate of OLV-I (OLV-1/Tk) possesses biological, morphological, physico-chemical, and ultrastructural properties similar, if not identical to those of the OLV-1 type strain and is also serologically indistinguishable from it. In addition, OLV-1/Tk has many properties, especially physico-chemical, in common with serotypes A and D of tobacco necrosis necrovirus (TNV-A and TNV-D). However, OLV-1/Tk is only very distantly related serologically to both TNV-A and D, suggesting that it can be regarded as a distinct species in the genus Necrovirus. OLV-1/Tk could not be detected in citrus tissues by ELISA or dot-blot molecular hybridization, probably because of the extremely low virus concentration. By contrast, limited virus recovery was obtained by sap inoculation and fair detection rates were afforded by PCR. OLV-1/Tk was identified in 54 of 92 (59%) citrus plants affected by CCD and in 14 of 49 (28%) symptomless plants. These results do not support the notion that there is a canse-effect relationship between OLV-1/Tk and CCD, even though the more frequent association of this virus with diseased plants remains intriguing.

EPPO Bulletin, 1998

Surveys were carried out in vineyards in the main grapevine-growing areas of Palestine (Hebron, B... more Surveys were carried out in vineyards in the main grapevine-growing areas of Palestine (Hebron, Bethlehem, Gaza, Jerusalem, Ramallah, Jenin, Jericho and Nablus) to assess the presence and incidence of virus and virus-like diseases. Leafroll symptoms were observed in Bethlehem, Ramallah and Jerusalem in native and imported cultivars, with higher rates in the red-fruited Shami, Beitoni and Smari. Rugose-wood symptoms were also observed in local and foreign cultivars, especially on grafted vines with a high incidence in Bethlehem. Fanleaf symptoms were rarely observed, while phytoplasma-induced symptoms were observed in Jenin, Jericho and Bethlehem on cvs Biadi, Superior Seedless and Beitoni. ELISA tests showed that 463 out of 566 (82%) tested vines were infected by at least one virus. GVA was the prevailing virus (66.1%), followed by GLRaV-1 (45.6%), GLRaV-3 (21.7%). GFkV (15.7%) and GLRaV-2 (8.3%). GVB and GFLV were also detected to a lesser extent, their incidence ranging between 3.7 and 1.2%. whereas GLRaV-7 was detected in a single vine of cv. Sultanina of foreign origin. Vineyards in the Bethlehem area were particularly badly damaged (97.5%). and some local cultivars were totally (Jandali, Marrawi and Shoyoukhi) or heavily infected (Zaini, Biadi and Shami). ELISA testing of 69 young rootstock mother plants showed a relatively high incidence of virus infection (20.3%).

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Atti delle Giornate Fitopatologiche, 2004

(English) In the framework of the programme of clonal and sanitary selection fostered by the Marc... more (English) In the framework of the programme of clonal and sanitary selection fostered by the Marches Region for the improvement of grapevine germplasm, 556 ecotypes belonging to 38 grapevine cultivars were assessed for sanitary status using immunoenzymatic assay. Investigations were focused on the main grapevine viruses (GVA, GVB, GFLV, GFkV, GLRaV-1, GLRaV-2, GLRaV-3 and GLRaV-7), for which serological reagents are available. In the cultivars Lacrima nera, Montepulciano, Passerina, Pecorino, ...

Le Progrès agricole et viticole HS, 16th Meeting ICVG, 2009

Sequence analyses of 15 genes from Bois noir phytoplasma strains affecting Italian vineyards unco... more Sequence analyses of 15 genes from Bois noir phytoplasma strains affecting Italian vineyards uncovered a range of new molecular markers. Emerging relationships between these markers and natural ecologies of BN disease suggest a possible role of tuf and hlyC genes in phytoplasma-plant interactions.