Vahid Noaman - Academia.edu (original) (raw)
Papers by Vahid Noaman
Background: Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world... more Background: Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world, being recognized to harbor and transmit a wide range of vector-borne diseases. Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato are zoonotic tick-borne pathogens causing emerging diseases. Wild animals play an essential role in the transmission of diseases and pathogens maintenance in nature. Epidemiological studies regarding the prevalence of tick-borne pathogens in red foxes are of public health importance, as they may successfully act as a pathogen transmission interface between wildlife, domestic animals and humans. Findings: This study included 14 counties from Romania. A total number of 353 red foxes (Vulpes vulpes) were examined. Heart tissue samples were collected during necropsy and stored at −20°C. Genomic DNA extraction was performed and all samples were examined by polymerase chain reaction (PCR). Specific primers for A. phagocytophilum, A. platys, E. canis and Borrelia burgdorferi s.l. were used. Sequence analysis was performed (Macrogen Europe, Amsterdam) and obtained sequences are available at GenBank™. Out of the 353 samples, 9 (2.55 %; 95 % CI: 1.25-4.96 %) were positive for A. phagocytophilum. Positive animals originated from 5 counties. In total, 5 out of 353 heart tissue samples (1.42 %; 95 % CI: 0.52-3.47 %) collected from red foxes were positive for B. burgdorferi s.l. Red foxes originated from 4 counties. None of the samples were positive for A. platys or E. canis. No co-infection with A. phagocytophilum and B. burgdorferi s.l. was found. Conclusion: This first report of A. phagocytophilum and B. burgdorferi s.l. in red foxes from Romania suggests a limited role of foxes in the maintenance of the two related pathogens, but may represent a potential risk from a public health perspective.
Journal of the Hellenic Veterinary Medical Society, Nov 9, 2022
Anaplasmosis is an important tick-borne disease caused by Anaplasma species in cattle and other r... more Anaplasmosis is an important tick-borne disease caused by Anaplasma species in cattle and other ruminants. This study aimed to determine the leukocytotropic species of bovine anaplasmosis (Anaplasma bovis and A. phagocytophilium) among cattle of Lorestan province, Iran using nested polymerase chain reaction (nPCR). Two hundred blood samples were taken from the jugular vein from healthy cattle, randomly. The extracted DNA from blood cells was screened using the universal primers, which amplify a DNA fragment (1468bp) from the 16S rRNA gene of eubacteria. The PCR products from positive samples were examined for A. bovis and A. phagocytophilium16S rRNA gene by species-specific nPCR.Out of the 200 cattle examined, 10 (5%) and 4 (2%) were found nPCR positive for A. bovis and A. phagocytophilium, respectively. No morulae were observed in the blood smears and clinical signs were not seen in infected cattle. Findings in the present study corroborate the low infection rate of A. bovis and A. phagocytophilum in Lorestan province, Iran. Identifying interactions among animal reservoirs, vectors, and pathogens in different climatic zones in Iran are necessary to be performed in future investigations.
The Scientific World Journal, 2013
lead to a high rate of cardiovascular mortality. In particular, multiple pathways including calci... more lead to a high rate of cardiovascular mortality. In particular, multiple pathways including calcium and phosphorus metabolic abnormalities caused by secondary hyperparathyroidism, inflammation and cardiac overload accelerate the process of valvular calcification, and the consequent P atients with end-stage renal disease (ESRD) on chronic hemodialysis (HD) often have a high prevalence of structural abnormalities of the heart including calcified valvular sclerosis, 1,2 left ventricular (LV) remodeling 3,4 and LV diastolic dysfunction, 5 which may
According to many reports, the beneficial effects of probiotic bacteria on fish have well been do... more According to many reports, the beneficial effects of probiotic bacteria on fish have well been documented. Probiotics as an alternative strategy have been suggested to be used as replacement for antimicrobial drugs and growth promoters. In addition, some researchers believe that probiotics have advantages for improving the health of fish in aquaculture and increasing fish performance. Probiotic bacteria can be used in feed and play a beneficial role in the ecosystem of the fish gastrointestinal tract and finally enhance the growth performance and other rearing parameters such as feed conversion ratio, protein efficiency ratio, digestibility and body composition.
JOURNAL OF VETERINARY MICROBIOLOGY, 2017
Journal of Children's Orthopaedics, 2018
archives of razi institute, 2014
It is about 50 years that FMD affected the ruminants of Isfahan. Last outbreaks of FMD were happe... more It is about 50 years that FMD affected the ruminants of Isfahan. Last outbreaks of FMD were happened at 2005 even vaccinated animals, so in current work using RT-PCR, sequencing and regression "r" values, the isolated strains in Isfahan were identified. The aim of this study was molecular epidemiology of FMDV in Isfahan province as the central part of Iran in 2006-2009. According to the result , a highly pathogen A05 strain was isolated from west (Najafabad city) about 2 months after the entrance of this virus to Iran through the west and north west margins toward central part and then distributed around 10 cities of Isfahan province. Here it is obvious that the A05 strain of Isfahan just showed 1% difference with A05IR (vaccine strain), in which for A22 were 65 %. Also based on the alignment of 600 bp of 3΄ end of the VP1 sequences of isolated type O comparing with representative of type O Shabestar vaccine strain and the other provinces of Iran, the Isfahan O isolate was...
گونه آناپلاسما فاگوسیتوفیلوم یکیاز باکتریهای بیماریزای نوپدید منتقله توسط کنهها میباشد که باع... more گونه آناپلاسما فاگوسیتوفیلوم یکیاز باکتریهای بیماریزای نوپدید منتقله توسط کنهها میباشد که باعث ایجاد بیماری مشترک بین انسان و بسیاری از حیوانات میگردد و به همین دلیل اهمیت آن در بهداشت عمومی جامعه قابلتوجه میباشد. هدف از انجام مطالعه حاضر، شناسایی مولکولی گونه آناپلاسما فاگوسیتوفیلوم در گاوها و گوسفندان استان گیلان بود. بدین منظور در طول سال 1397، تعداد 200 نمونه خون از طریق رگ وداج گاوها و 200 نمونه خون از طریق رگ وداج گوسفندان، بهطور تصادفی از نقاط مختلف استان گیلان جمعآوری گردید. جهت آزمایش PCR (polymerase chain reaction)، در ابتدا DNA استخراجی از نمونههای خونی، با جفت آغازگری که قطعه 1468 جفت بازی از ژن 16SrRNA جنس آناپلاسما را تکثیر میکند، تکثیر دادهشد. 70 نمونه مثبت اولیه گاوی با استفاده از روش nested-PCR از نظر وجود گونه آناپلاسما فاگوسیتوفیلوم بررسی شدند و در نتیجه 66 نمونه (33 درصد)، مثبت تشخیص داده شد. همچنین 38 نمونه مثبت اولیه گوسفندی با استفاده از روش nested-PCR، ازنظر وجود گونه آناپلاسما فاگوسیتوفیلوم بررسی شدند که 10 نمونه (5 درصد)، از این نظرمثبت تشخ...
مطالعه حاضر با هدف بررسی مولکولی توزیع اپیدمیولوژیک بابزیا بوویس و بابزیا بایژمینا و عوامل خطر مر... more مطالعه حاضر با هدف بررسی مولکولی توزیع اپیدمیولوژیک بابزیا بوویس و بابزیا بایژمینا و عوامل خطر مربوطه در تعدادی از گاوهای منطقه شمالغرب ایران طراحی گردید. بدین منظور، 153 نمونه خون بهطور تصادفی از گاوهای استانهای آذربایجانشرقی و آذربایجانغربی جمعآوری شد. پس از استخراج DNAسلولهای خونی، یک جفت پرایمر حاصل از ژن18s rRNA برای شناسایی اعضای جنس بابزیا با واکنش زنجیرهای پلیمراز (polymerase chain reaction; PCR) مورد استفاده قرار گرفت.به منظور شناسایی گونههای بابزیا بوویس و بابزیا بایژمینا از تکنیک PCRنیمهآشیانهای (semi-nested PCR) استفاده شد. براساس یافتههای این مطالعه، از مجموع 153 نمونه خونی، 39 نمونه (49/25 درصد) آلوده به گونهای از انگل تکیاخته بابزیا بودند که یکی از نمونهها بهطور همزمان به دو گونه بابزیا آلوده بود. همچنین گونههای بابزیا بوویس و بابزیا بایژمینا به ترتیب در 38 و 2 مورد از نمونهها شناسایی شد. نتایج حاکی از میزان بالای شیوع آلودگی به بابزیا بوویس نسبت به بابزیا بایژمینا در دامهای تحتآزمایش بود (83/24 درصد در مقابل 30/1 درصد). از طرف دیگر، میزا...
Background: Trace elements are very important for several metabolic functions and their deficienc... more Background: Trace elements are very important for several metabolic functions and their deficiency impair production and reproduction in dairy cattle. The aim of this study was to evaluate the concentrations of Cu, Zn and Fe in diet samples in Holstein dairy cattle kept under industrial and semi-industrial farming in Isfahan province, central Iran. Materials and Methods: This study was carried out on 12 industrial and 12 semi-industrial Holstein dairy cattle farms. A total of 48 diet samples were collected. In the industrial farms, diet samples were collected from the total mixed rations (TMR) and in the semi-industrial farms, samples were separately collected from forage and concentrates. Concentrations of Cu, Zn, Fe in the cattle diet samples were determined using the atomic absorption spectrophotometry. Results: In the industrial farms, the mean concentrations of Cu, Zn and Fe in diet samples were 13 mg/Kg, 73 mg/Kg and 494 mg/Kg, respectively and in the semi-industrial farms, me...
Iranian Journal of Fisheries Sciences, 2021
Bivalvia is one of the least studied classes of the Persian Gulf. A survey and molecular analysis... more Bivalvia is one of the least studied classes of the Persian Gulf. A survey and molecular analysis was conducted to determine bivalve species diversity in the Persian Gulf. To the best of our knowledge, this is the first report of barcoding data on bivalves of the Persian Gulf. We examined 40 individuals representing 8 species, 6 genera and 5 families. We collected samples from Hengam, Larak and Qeshm Islands and Bandar Lengeh in Persian Gulf, Iran. After DNA extraction, mitochondrial 16S ribosomal DNA (16SrDNA) and Cytochrome Oxidase subunit I were amplified by polymerase chain reaction (PCR). Based on obtained 16SrDNA and COI gene sequences and maximum parsimony, neighbor joining and maximum likelihood trees of these genes there was no overlap between maximum Kimura 2- parameter distance among conspecifics. Most species formed agglutinate sequence units with a small amount of changes. Eventually, comparison of the 8 selected studied species with metadata from India, Brazil, Japan, ...
Journal of the Hellenic Veterinary Medical Society, 2020
Toxoplasmosis is considered an important zoonosis that affects not only humans but also livestock... more Toxoplasmosis is considered an important zoonosis that affects not only humans but also livestock worldwide. Small ruminants are important intermediate hosts of T. gondii. This study aimed to determine the molecular prevalence of T. gondii in the slaughtered sheep by loop-mediated isothermal amplification (LAMP) assay in Isfahan, Iran. A total of 250 sheep heart samples were collected from five different slaughterhouses in Isfahan, Iran, from October 2017 to May 2018. The LAMP assay was optimized targeting the sequence of 529 bp DNA fragments of T. gondii and detected T. gondii DNA in 96.4% (241/250) of samples. A significantly higher prevalence of T. gondii was recorded among female animals as compared to male animals (p ≤ 0.05). No statistically significant difference in T. gondii prevalence was noted between the seasons, type of animals, and age groups (p >0.05). To the best of our knowledge, the current study is the first to use the LAMP assay to determine the prevalence of T...
Advances in Bioengineering, 2004
Blood flow through the constricted area of a severe stenosis is similar to that through a venturi... more Blood flow through the constricted area of a severe stenosis is similar to that through a venturi or flow nozzle. In the contraction section, the blood can accelerate to high speed. In this situation, the external pressure may be greater than the internal fluid pressure, and the artery could collapse. This collapse may be important in the development of atherosclerotic plaque fracture and subsequent thrombosis or distal embolization. We have developed an experimental model that closely approximates the arterial disease situation and performed steady and pulsatile flow experiments, and the characteristics of flow and compression concerned with the geometry and compliance of the model were discussed [1]. But we had not focused on the coronary stenosis. As the first step to develop the experimental model of coronary stenosis, the curved stenosis model was made. This paper was to examine the influence of curvature and stretch of the stenosis model on flow and collapse under pulsatile flow.
To the best of our knowledge, little information is available regarding the presence of Anaplasma... more To the best of our knowledge, little information is available regarding the presence of Anaplasma species in camels in Iran. This study sought to investigate the presence of Anaplasma species by microscopy and polymerase chain reaction (PCR) assays in 100 healthy dromedaries (Camelus dromedarius) arriving for slaughter. The microscopic examination of Giemsa-stained blood films revealed that Anaplasma like structures could be identified in the erythrocytes of two blood smears. To confirm the presence of and to identify the species of Anaplasma spp., a PCR technique was performed using primers amplifying a 750 bp fragment of the 16S rRNA gene of Anaplasma and the PCR products were analyzed by sequencing. The nucleotide sequence was compared to the sequences available in GenBank using the Basic Local Alignment Search Tool (BLAST). According to the results, the sequences of two 16S rRNA PCR products clearly fit within the Anaplasma genus in the family Anaplas mataceae. In this study, ph...
Tropical Animal Health and Production
The aim of this cross-sectional study was to determine the molecular prevalence and associated ri... more The aim of this cross-sectional study was to determine the molecular prevalence and associated risk factors in sheep populations of Iran. To this end, between March 2017 and February 2018 jugular vein blood samples were collected from 1842 apparently healthy sheep from 327 herds in nine provinces in four ecological zones of Iran. A specific nested-PCR targeting the msp4 gene of A. ovis was employed. Fourteen variables were subjected to logistic regression analyses (univariate and multivariate) to specify the potential risk factors for infection. Statistically significant variables in univariate analyses (P ≤ 0.20) were assessed by multivariable logistic regression to control the confounding factors. Anaplasma ovis DNA was detected in 51.1% of herds (167/327) and 28.3% of animals (521/1842). Among geographical zones, herd and animal prevalence was highest in the Persian-Gulf zone (P < 0.001), and among provinces, Lorestan (in west) and Khuzestan (in south-west) had the highest prevalence (P < 0.001). Analysis of factors associated with A. ovis infection revealed that distance from other farms (OR = 2.52, P < 0.001), presence of other animal species in the farm (OR = 2.03, P = 0.046), season (OR = 1.40, P = 0.005), breed (OR = 3.762, P < 0.001), and age of sheep (OR = 1.20, P = 0.049) are potential risks in Iran. The spatial scan statistic in SaTScan recognized two high risks clusters for A. ovis infection in central (Semnan province) and the Persian-Gulf (Khuzestan province) zones amongst the study areas (P < 0.001). Sequence and phylogenetic analysis of the msp4 gene confirmed the detection of A. ovis. This research is the largest study focusing on ovine anaplasmosis in Iran and shows that infected sheep are present in all geographic zones, bioclimatic areas, and provinces.
Background: Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world... more Background: Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world, being recognized to harbor and transmit a wide range of vector-borne diseases. Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato are zoonotic tick-borne pathogens causing emerging diseases. Wild animals play an essential role in the transmission of diseases and pathogens maintenance in nature. Epidemiological studies regarding the prevalence of tick-borne pathogens in red foxes are of public health importance, as they may successfully act as a pathogen transmission interface between wildlife, domestic animals and humans. Findings: This study included 14 counties from Romania. A total number of 353 red foxes (Vulpes vulpes) were examined. Heart tissue samples were collected during necropsy and stored at −20°C. Genomic DNA extraction was performed and all samples were examined by polymerase chain reaction (PCR). Specific primers for A. phagocytophilum, A. platys, E. canis and Borrelia burgdorferi s.l. were used. Sequence analysis was performed (Macrogen Europe, Amsterdam) and obtained sequences are available at GenBank™. Out of the 353 samples, 9 (2.55 %; 95 % CI: 1.25-4.96 %) were positive for A. phagocytophilum. Positive animals originated from 5 counties. In total, 5 out of 353 heart tissue samples (1.42 %; 95 % CI: 0.52-3.47 %) collected from red foxes were positive for B. burgdorferi s.l. Red foxes originated from 4 counties. None of the samples were positive for A. platys or E. canis. No co-infection with A. phagocytophilum and B. burgdorferi s.l. was found. Conclusion: This first report of A. phagocytophilum and B. burgdorferi s.l. in red foxes from Romania suggests a limited role of foxes in the maintenance of the two related pathogens, but may represent a potential risk from a public health perspective.
Journal of the Hellenic Veterinary Medical Society, Nov 9, 2022
Anaplasmosis is an important tick-borne disease caused by Anaplasma species in cattle and other r... more Anaplasmosis is an important tick-borne disease caused by Anaplasma species in cattle and other ruminants. This study aimed to determine the leukocytotropic species of bovine anaplasmosis (Anaplasma bovis and A. phagocytophilium) among cattle of Lorestan province, Iran using nested polymerase chain reaction (nPCR). Two hundred blood samples were taken from the jugular vein from healthy cattle, randomly. The extracted DNA from blood cells was screened using the universal primers, which amplify a DNA fragment (1468bp) from the 16S rRNA gene of eubacteria. The PCR products from positive samples were examined for A. bovis and A. phagocytophilium16S rRNA gene by species-specific nPCR.Out of the 200 cattle examined, 10 (5%) and 4 (2%) were found nPCR positive for A. bovis and A. phagocytophilium, respectively. No morulae were observed in the blood smears and clinical signs were not seen in infected cattle. Findings in the present study corroborate the low infection rate of A. bovis and A. phagocytophilum in Lorestan province, Iran. Identifying interactions among animal reservoirs, vectors, and pathogens in different climatic zones in Iran are necessary to be performed in future investigations.
The Scientific World Journal, 2013
lead to a high rate of cardiovascular mortality. In particular, multiple pathways including calci... more lead to a high rate of cardiovascular mortality. In particular, multiple pathways including calcium and phosphorus metabolic abnormalities caused by secondary hyperparathyroidism, inflammation and cardiac overload accelerate the process of valvular calcification, and the consequent P atients with end-stage renal disease (ESRD) on chronic hemodialysis (HD) often have a high prevalence of structural abnormalities of the heart including calcified valvular sclerosis, 1,2 left ventricular (LV) remodeling 3,4 and LV diastolic dysfunction, 5 which may
According to many reports, the beneficial effects of probiotic bacteria on fish have well been do... more According to many reports, the beneficial effects of probiotic bacteria on fish have well been documented. Probiotics as an alternative strategy have been suggested to be used as replacement for antimicrobial drugs and growth promoters. In addition, some researchers believe that probiotics have advantages for improving the health of fish in aquaculture and increasing fish performance. Probiotic bacteria can be used in feed and play a beneficial role in the ecosystem of the fish gastrointestinal tract and finally enhance the growth performance and other rearing parameters such as feed conversion ratio, protein efficiency ratio, digestibility and body composition.
JOURNAL OF VETERINARY MICROBIOLOGY, 2017
Journal of Children's Orthopaedics, 2018
archives of razi institute, 2014
It is about 50 years that FMD affected the ruminants of Isfahan. Last outbreaks of FMD were happe... more It is about 50 years that FMD affected the ruminants of Isfahan. Last outbreaks of FMD were happened at 2005 even vaccinated animals, so in current work using RT-PCR, sequencing and regression "r" values, the isolated strains in Isfahan were identified. The aim of this study was molecular epidemiology of FMDV in Isfahan province as the central part of Iran in 2006-2009. According to the result , a highly pathogen A05 strain was isolated from west (Najafabad city) about 2 months after the entrance of this virus to Iran through the west and north west margins toward central part and then distributed around 10 cities of Isfahan province. Here it is obvious that the A05 strain of Isfahan just showed 1% difference with A05IR (vaccine strain), in which for A22 were 65 %. Also based on the alignment of 600 bp of 3΄ end of the VP1 sequences of isolated type O comparing with representative of type O Shabestar vaccine strain and the other provinces of Iran, the Isfahan O isolate was...
گونه آناپلاسما فاگوسیتوفیلوم یکیاز باکتریهای بیماریزای نوپدید منتقله توسط کنهها میباشد که باع... more گونه آناپلاسما فاگوسیتوفیلوم یکیاز باکتریهای بیماریزای نوپدید منتقله توسط کنهها میباشد که باعث ایجاد بیماری مشترک بین انسان و بسیاری از حیوانات میگردد و به همین دلیل اهمیت آن در بهداشت عمومی جامعه قابلتوجه میباشد. هدف از انجام مطالعه حاضر، شناسایی مولکولی گونه آناپلاسما فاگوسیتوفیلوم در گاوها و گوسفندان استان گیلان بود. بدین منظور در طول سال 1397، تعداد 200 نمونه خون از طریق رگ وداج گاوها و 200 نمونه خون از طریق رگ وداج گوسفندان، بهطور تصادفی از نقاط مختلف استان گیلان جمعآوری گردید. جهت آزمایش PCR (polymerase chain reaction)، در ابتدا DNA استخراجی از نمونههای خونی، با جفت آغازگری که قطعه 1468 جفت بازی از ژن 16SrRNA جنس آناپلاسما را تکثیر میکند، تکثیر دادهشد. 70 نمونه مثبت اولیه گاوی با استفاده از روش nested-PCR از نظر وجود گونه آناپلاسما فاگوسیتوفیلوم بررسی شدند و در نتیجه 66 نمونه (33 درصد)، مثبت تشخیص داده شد. همچنین 38 نمونه مثبت اولیه گوسفندی با استفاده از روش nested-PCR، ازنظر وجود گونه آناپلاسما فاگوسیتوفیلوم بررسی شدند که 10 نمونه (5 درصد)، از این نظرمثبت تشخ...
مطالعه حاضر با هدف بررسی مولکولی توزیع اپیدمیولوژیک بابزیا بوویس و بابزیا بایژمینا و عوامل خطر مر... more مطالعه حاضر با هدف بررسی مولکولی توزیع اپیدمیولوژیک بابزیا بوویس و بابزیا بایژمینا و عوامل خطر مربوطه در تعدادی از گاوهای منطقه شمالغرب ایران طراحی گردید. بدین منظور، 153 نمونه خون بهطور تصادفی از گاوهای استانهای آذربایجانشرقی و آذربایجانغربی جمعآوری شد. پس از استخراج DNAسلولهای خونی، یک جفت پرایمر حاصل از ژن18s rRNA برای شناسایی اعضای جنس بابزیا با واکنش زنجیرهای پلیمراز (polymerase chain reaction; PCR) مورد استفاده قرار گرفت.به منظور شناسایی گونههای بابزیا بوویس و بابزیا بایژمینا از تکنیک PCRنیمهآشیانهای (semi-nested PCR) استفاده شد. براساس یافتههای این مطالعه، از مجموع 153 نمونه خونی، 39 نمونه (49/25 درصد) آلوده به گونهای از انگل تکیاخته بابزیا بودند که یکی از نمونهها بهطور همزمان به دو گونه بابزیا آلوده بود. همچنین گونههای بابزیا بوویس و بابزیا بایژمینا به ترتیب در 38 و 2 مورد از نمونهها شناسایی شد. نتایج حاکی از میزان بالای شیوع آلودگی به بابزیا بوویس نسبت به بابزیا بایژمینا در دامهای تحتآزمایش بود (83/24 درصد در مقابل 30/1 درصد). از طرف دیگر، میزا...
Background: Trace elements are very important for several metabolic functions and their deficienc... more Background: Trace elements are very important for several metabolic functions and their deficiency impair production and reproduction in dairy cattle. The aim of this study was to evaluate the concentrations of Cu, Zn and Fe in diet samples in Holstein dairy cattle kept under industrial and semi-industrial farming in Isfahan province, central Iran. Materials and Methods: This study was carried out on 12 industrial and 12 semi-industrial Holstein dairy cattle farms. A total of 48 diet samples were collected. In the industrial farms, diet samples were collected from the total mixed rations (TMR) and in the semi-industrial farms, samples were separately collected from forage and concentrates. Concentrations of Cu, Zn, Fe in the cattle diet samples were determined using the atomic absorption spectrophotometry. Results: In the industrial farms, the mean concentrations of Cu, Zn and Fe in diet samples were 13 mg/Kg, 73 mg/Kg and 494 mg/Kg, respectively and in the semi-industrial farms, me...
Iranian Journal of Fisheries Sciences, 2021
Bivalvia is one of the least studied classes of the Persian Gulf. A survey and molecular analysis... more Bivalvia is one of the least studied classes of the Persian Gulf. A survey and molecular analysis was conducted to determine bivalve species diversity in the Persian Gulf. To the best of our knowledge, this is the first report of barcoding data on bivalves of the Persian Gulf. We examined 40 individuals representing 8 species, 6 genera and 5 families. We collected samples from Hengam, Larak and Qeshm Islands and Bandar Lengeh in Persian Gulf, Iran. After DNA extraction, mitochondrial 16S ribosomal DNA (16SrDNA) and Cytochrome Oxidase subunit I were amplified by polymerase chain reaction (PCR). Based on obtained 16SrDNA and COI gene sequences and maximum parsimony, neighbor joining and maximum likelihood trees of these genes there was no overlap between maximum Kimura 2- parameter distance among conspecifics. Most species formed agglutinate sequence units with a small amount of changes. Eventually, comparison of the 8 selected studied species with metadata from India, Brazil, Japan, ...
Journal of the Hellenic Veterinary Medical Society, 2020
Toxoplasmosis is considered an important zoonosis that affects not only humans but also livestock... more Toxoplasmosis is considered an important zoonosis that affects not only humans but also livestock worldwide. Small ruminants are important intermediate hosts of T. gondii. This study aimed to determine the molecular prevalence of T. gondii in the slaughtered sheep by loop-mediated isothermal amplification (LAMP) assay in Isfahan, Iran. A total of 250 sheep heart samples were collected from five different slaughterhouses in Isfahan, Iran, from October 2017 to May 2018. The LAMP assay was optimized targeting the sequence of 529 bp DNA fragments of T. gondii and detected T. gondii DNA in 96.4% (241/250) of samples. A significantly higher prevalence of T. gondii was recorded among female animals as compared to male animals (p ≤ 0.05). No statistically significant difference in T. gondii prevalence was noted between the seasons, type of animals, and age groups (p >0.05). To the best of our knowledge, the current study is the first to use the LAMP assay to determine the prevalence of T...
Advances in Bioengineering, 2004
Blood flow through the constricted area of a severe stenosis is similar to that through a venturi... more Blood flow through the constricted area of a severe stenosis is similar to that through a venturi or flow nozzle. In the contraction section, the blood can accelerate to high speed. In this situation, the external pressure may be greater than the internal fluid pressure, and the artery could collapse. This collapse may be important in the development of atherosclerotic plaque fracture and subsequent thrombosis or distal embolization. We have developed an experimental model that closely approximates the arterial disease situation and performed steady and pulsatile flow experiments, and the characteristics of flow and compression concerned with the geometry and compliance of the model were discussed [1]. But we had not focused on the coronary stenosis. As the first step to develop the experimental model of coronary stenosis, the curved stenosis model was made. This paper was to examine the influence of curvature and stretch of the stenosis model on flow and collapse under pulsatile flow.
To the best of our knowledge, little information is available regarding the presence of Anaplasma... more To the best of our knowledge, little information is available regarding the presence of Anaplasma species in camels in Iran. This study sought to investigate the presence of Anaplasma species by microscopy and polymerase chain reaction (PCR) assays in 100 healthy dromedaries (Camelus dromedarius) arriving for slaughter. The microscopic examination of Giemsa-stained blood films revealed that Anaplasma like structures could be identified in the erythrocytes of two blood smears. To confirm the presence of and to identify the species of Anaplasma spp., a PCR technique was performed using primers amplifying a 750 bp fragment of the 16S rRNA gene of Anaplasma and the PCR products were analyzed by sequencing. The nucleotide sequence was compared to the sequences available in GenBank using the Basic Local Alignment Search Tool (BLAST). According to the results, the sequences of two 16S rRNA PCR products clearly fit within the Anaplasma genus in the family Anaplas mataceae. In this study, ph...
Tropical Animal Health and Production
The aim of this cross-sectional study was to determine the molecular prevalence and associated ri... more The aim of this cross-sectional study was to determine the molecular prevalence and associated risk factors in sheep populations of Iran. To this end, between March 2017 and February 2018 jugular vein blood samples were collected from 1842 apparently healthy sheep from 327 herds in nine provinces in four ecological zones of Iran. A specific nested-PCR targeting the msp4 gene of A. ovis was employed. Fourteen variables were subjected to logistic regression analyses (univariate and multivariate) to specify the potential risk factors for infection. Statistically significant variables in univariate analyses (P ≤ 0.20) were assessed by multivariable logistic regression to control the confounding factors. Anaplasma ovis DNA was detected in 51.1% of herds (167/327) and 28.3% of animals (521/1842). Among geographical zones, herd and animal prevalence was highest in the Persian-Gulf zone (P < 0.001), and among provinces, Lorestan (in west) and Khuzestan (in south-west) had the highest prevalence (P < 0.001). Analysis of factors associated with A. ovis infection revealed that distance from other farms (OR = 2.52, P < 0.001), presence of other animal species in the farm (OR = 2.03, P = 0.046), season (OR = 1.40, P = 0.005), breed (OR = 3.762, P < 0.001), and age of sheep (OR = 1.20, P = 0.049) are potential risks in Iran. The spatial scan statistic in SaTScan recognized two high risks clusters for A. ovis infection in central (Semnan province) and the Persian-Gulf (Khuzestan province) zones amongst the study areas (P < 0.001). Sequence and phylogenetic analysis of the msp4 gene confirmed the detection of A. ovis. This research is the largest study focusing on ovine anaplasmosis in Iran and shows that infected sheep are present in all geographic zones, bioclimatic areas, and provinces.