Valcineide Tanobe - Academia.edu (original) (raw)

Papers by Valcineide Tanobe

Polymer Testing, 2005

... Natural fibers, from leaves, seed or bast include coir, sisal, jute, sponge-gourd (Luffa cyli... more ... Natural fibers, from leaves, seed or bast include coir, sisal, jute, sponge-gourd (Luffa cylindrica), flax, ramie, abaca, kenaf, cotton, palmyra, bamboo, mesta, henequen, istle, kapok, hemp, sunn, piassava, wood, banana, kusha, sawai grasses, pineapple, etc. ...

Wool Fiber Reinforced Polymer Composites

Nutrients in Dairy and their Implications on Health and Disease, 2017

Abstract Immunoglobulins (Igs) are glycoprotein molecules produced by plasma cells that act as a ... more Abstract Immunoglobulins (Igs) are glycoprotein molecules produced by plasma cells that act as a critical part of the immune response by specifically recognizing and binding to particular antigens, such as viruses, protozoa, bacteria, and toxins. Dairy fluids provide all known classes of Igs (i.e., IgA, IgD, IgE, IgG, and IgM) with antibody activities against various microorganisms. These Ig classes are found in high concentrations in colostrum—a form of low-volume milk secreted by most mammals during the first days of postpartum lactation. The growing interest in bovine Igs as therapeutics and functional foods has provoked the need for innovative process technologies for their isolation, purification, and stabilization. The purified Igs could be intended for the neonate of the species producing the secretion, or for a different species, such as humans. This chapter reviews the Ig composition of milk and colostrum in different species of dairy animals and stages of lactation, and presents the mechanism for transfer of passive immunity as well as potential health promotion. In addition, this chapter describes recent developments in Ig isolation, concentration, purification, and stabilization.

In Vitro Cellular & Developmental Biology - Animal, 2021

This study presents an in vitro evaluation of the antitumor potential of a chitin-like exopolysac... more This study presents an in vitro evaluation of the antitumor potential of a chitin-like exopolysaccharide (EPS, produced by Mortierella alpina) on Adrenocortical carcinoma cells (ACC) compared to mitotane, a commercial drug commonly used in ACC treatment, and known for its side effects. Techniques of cellular viability determination such as MTT and fluorescence were used to measure the cytotoxic effects of the EPS and mitotane in tumoral cells (H295R) and non-tumoral cells (VERO), observing high cytotoxicity of mitotane and a 10% superior pro-apoptotic effect of the EPS compared to mitotane (p < 0.05). The cytotoxic effect of the EPS was similar to the effect of 50 μM mitotane on tumoral cells (p < 0.05). A decrement of the lysosomal volume was also noted in tumoral cells treated with the EPS. To enhance the antitumor effect, a combination of mitotane at a lower dosage and the EPS (as adjuvant) was also tested, showing a slight improvement of the cytotoxicity effect on tumoral cells. Therefore, the results indicate a cytotoxic effect of the EPS produced by Mortierella alpina on adrenocortical carcinoma, and a possible application in biomedical formulations or additional treatments.

Cellulose, 2021

Lately, cocoa pod husk (CPH) has received some attention from researchers due to its significant ... more Lately, cocoa pod husk (CPH) has received some attention from researchers due to its significant content of cellulose, hemicelluloses and pectin, antioxidant capacity, potential for energy generation, physicochemical characteristics and possibility to be used as adsorbent material or activated carbon. In this work, alkaline (NaOH 2.3% w/v) and enzymatic treatment [Cellic® CTec 2 (2.4% v/v)] were developed before propionic acid (PA) production using fermentable sugars derived from cocoa pod husk hydrolysate (CPHH). The physical, structural and morphological characteristics of raw and treated samples of CPH lignocellulosic matrix were assessed using tools such as scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetry, and X-ray diffraction. The results of these analyses revealed the effectiveness of CPH sequential alkaline and enzymatic treatment for glucose production, reaching maximum concentration of 60.5 g·L−1 and a yield of 275 mg glucose·g−1 CPH. Subsequently, a medium composed by glucose from CPHH (7.5 g·L−1), as low-cost feedstock, glycerol (7.5 g·L−1) and yeast extract (10 g·L−1), was prepared to obtain PA using Propionibacterium jensenii DSM 20274. PA concentration reached a maximum of 10.28 ± 1.05 g·L−1 and a productivity of 0.08 ± 0.01 g·L−1·h−1 after 72 h of fermentation. To the best of our knowledge, there is no literature available on the bioconversion of CPH for the specific production of PA or other organic acids. Thus, the potential of CPH to be used as substrate for PA production was demonstrated with good perspectives.

Environmental Technology, 2020

Cellulose is a potential resource to be recovered from wastewater treatment plants (WWTP). Enzyme... more Cellulose is a potential resource to be recovered from wastewater treatment plants (WWTP). Enzyme formulations can be employed to hydrolyze cellulose into fermentable sugars, to be further used as biochemical building blocks or reducing its recalcitrance to further treatment processes. This study proposed the production, recovery and formulation of cellulase using domestic wastewater as culture medium and its application for the hydrolysis of cellulosic residues recovered from WWTPs. Cellulose was recovered from raw sanitary wastewater using a fine-mesh sieve (0.35 mm) and quantified through enzymatic hydrolysis and thermogravimetric analysis. The production, concentration and formulation of cellulase enzyme resulted in an enzymatic blend of endoglucanases (7.3 UFP/mL), cellobiohydrolases (7.4 UCMC/mL) and beta-glucosidases (4.4 UBGL/mL). The content of the recovered cellulosic material was 21.3% according to enzymatic hydrolysis and 27.7 for thermogravimetric results. The enzymatic hydrolysis of the WWTP residue using the produced cellulase (107.6 ± 10.2 mgreduc/gresidue) showed better results than using the commercial cellulase complex (66.4 ± 2.5 mgreduc/gresidue). This fact showed the potential of application of the produced enzyme for the hydrolysis of cellulosic residues recovered from WWTP processes. In a non-waste biorefinery approach, the generated hydrolysate can be further used for producing added-value biomolecules including biofuels and biochemicals.

Bioresource Technology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Applied Biochemistry and Biotechnology, 2019

Raw domestic wastewater was used as a culture medium for cellulase production in a bubble column ... more Raw domestic wastewater was used as a culture medium for cellulase production in a bubble column reactor (6.2 U FP /mL, 64.6 U/L h) using the strain Trichoderma harzianum TRIC03-LPBII. Cellulases presented optimum pH and temperature between 4 and 5 and 50 and 70°C, respectively. Enzymatic extract was concentrated through ultrafiltration and then a cellulolytic formulation was prepared with the addition of sorbitol (50% w/v) and benzoic acid (0.05% w/v). High cellulase stability of around 100% was reached after 30 days at 4°C. The concentrated extract was also dried in a spray-dryer with the addition of maltodextrin at 20% (w/v), resulting in powder enzymatic formulation with 85% stability after 60 days. With these characteristics, the liquid and powder cellulase products have potential to be used in different industrial applications.

Fermentation, 2017

The aim of this study was to evaluate the yeast diversity and physicochemical characteristics of ... more The aim of this study was to evaluate the yeast diversity and physicochemical characteristics of spontaneous coffee bean fermentation conducted in the coffee-producing region in Cerrado Mineiro, Brazil. During 48 h of fermentation, yeast population increased from 6.60 to 7.89 log CFU•g −1 , with concomitant pulp sugar consumption and organic acids production (mainly lactic (3.35 g•L −1) and acetic (1.27 g•L −1) acids). According to ITS-rRNA gene sequencing, yeast population was mainly represented by Saccharomyces sp., followed by Torulaspora delbrueckii, Pichia kluyveri, Hanseniaspora uvarum, H. vineae and Meyerozyma caribbica. SPME-GC-MS analysis revealed a total of 25 volatile organic compounds with predominance of hydrocarbons (9 compounds) and higher alcohols (6 compounds). The resulting fermented, roasted coffee beans were analyzed by diverse chemical analysis methods, including Fourier Transform Infrared (FTIR) spectroscopy and mineral and thermogravimetric analysis. The thermal decomposition of the coffee beans occurred in four stages between 90 and 390 • C, with significant mass loss (68%) after the second stage at 190 • C. FTIR spectroscopy confirmed the presence of the main organic functions associated with coffee aroma, such as aromatic acids, ketones, aldehydes and aliphatic esters. The results presented in this study enrich our knowledge concerning yeast diversity and physicochemical characteristics associated with coffee bean fermentation, and can be used to promote a controlled on-farm processing.

LWT, 2017

The use of selected yeasts has recently been proposed as an interesting strategy to improve cocoa... more The use of selected yeasts has recently been proposed as an interesting strategy to improve cocoa bean fermentation and chocolate quality. In this study, a total of 39 cocoa-derived yeast isolates were screened for their capacity to produce volatile aroma compounds in a cocoa pulp simulation medium. The seven highest aroma-producing yeasts were identified by ITS-rRNA gene sequencing as belonging to Pichia kudriavzevii, in spite of exhibiting different metabolic profiles. Repetitive extragenic palindromic (Rep)-PCR genomic fingerprinting approach confirmed this high intraspecies diversity of P. kudriavzevii in cocoa fermentation. Two strains with superior aroma production, namely P. kudriavzevii LPB06 and P. kudriavzevii LPB07, were used in cocoa beans fermentation at laboratory scale. They were able to establish an accelerated fermentation process with efficient yeast growth, sugars consumption and ethanol formation compared to the spontaneous process. The resulting cocoa beans were analyzed by diverse chemical analysis methods, including gas ghromatography coupled to mass spectrophotometry (GC-MS), fermentation index and metal and colorimetric analysis. All together, the results indicated that inoculated fermentations generated cocoa beans with better color development and richer aroma composition, suggesting that cocoa-associated yeast diversity at strain level can be exploited for flavor modulation of cocoa beans.

Journal of Cleaner Production, 2017

The regeneration of activated vegetable carbon applied to hydrotreated diesel in order adsorb sul... more The regeneration of activated vegetable carbon applied to hydrotreated diesel in order adsorb sulphur and nitrogen compounds, as a complementary process to hydrotreating, was studied under various conditions. The activated carbon from babassu by-products was investigated both in its original form (AC) and impregnated with copper (Cu II /AC), and the influence of impregnation on the adsorption and regeneration processes was subsequently compared. The impregnated carbon was shown to be very efficient in the removal of both sulphur and nitrogen compounds, removing up to 94% of nitrogen content present in the diesel. The capacity of the carbon for regeneration was also studied, initially using four individual solvents (acetone, ethanol, benzene and toluene), of which toluene showed the highest recovery for both adsorbents, enabling a recovery of adsorption capacity R (%) of 64.7% for sulphur compounds and 83.3% for nitrogen compounds. Regeneration conditions were optimized, the best results being obtained at 40 C. Furthermore, both adsorbents underwent consecutive cycles of adsorption and regeneration, and after the fifth adsorption it was still possible to remove 28% of the sulphur present in the diesel using the AC carbon and 70% of the nitrogen using the impregnated carbon. These experiments indicate that from both an environmental and an industrial standpoint, the use of activated carbon from by-products generated during babassu oil extraction as an adsorbent appears to be a promising alternative for Brazilian refineries, which may be applied as a complementary process to hydrotreatment in order to obtain a cleaner and less polluting diesel fuel.

Green Fuels Technology, 2016

Considering the great interest of the countries around the world and the great number of research... more Considering the great interest of the countries around the world and the great number of research of many groups from Institutes, Universities, Companies, aiming to allow the use of biomass to produce biofuel, this chapter may give data and basic information about vegetable cultures and organic residues available to generate any kind of biofuel. Concerning to this a general view is given about crops where its main constituent component is sugar, starch, oil, with some principal examples. Besides, data, facilities and examples are given about lignocellulolytic cultures and residues, as far as solid and liquid residues generated in some industrial processes. The data available, includes, amount of each potential energetic source of raw material for biofuel, physic-chemical characteristics, production region, agronomic data, productivity, production, and other information useful for researchers works aiming the production of bioethanol, biodiesel, and biohydrogen.

Anais do XX Congresso Brasileiro de Engenharia Química, 2015

Matéria (Rio de Janeiro), 2010

Increasing search for new materials with high premium on eco-friendliness, new trend is emerging ... more Increasing search for new materials with high premium on eco-friendliness, new trend is emerging in materials development such as composites, which are well established for a wide variety of applications. With growing interest and importance of renewable bioresources has led to more stress on the use of locally available materials. This paper presents preliminary results on the preparation and characterization of composites based on Brazilian coconut fibers and starches of cassava and corn. The raw materials were characterized for their morphology, chemical composition, and thermal properties and X-ray diffraction studies. Coir fibers were also tested for their tensile properties showing increasing strength and Young`s modulus with decreasing diameter, while the % elongation remaining constant. Lignin content of coir was found to be 35%. Structure and properties of composites containing 0, 5 10, 15% fibers in both the matrices and prepared by compression molding would be compared. For the 2 types of starch, there was an increase in the tensile strength by the increasing proportion of fiber. The effect of moisture in the composite stress affects the strength and percentage elongation. The water absorption was higher in the composites made from cassava starch.

Journal of Medicinal Plants Research, 2012

The antibacterial and antifungal activities of water and chloroform extracts of Acorus calamus, A... more The antibacterial and antifungal activities of water and chloroform extracts of Acorus calamus, Aremisia annua, Chenopodium foloisom, Euphobia heliscopia and Cupressus sempervirens were carried out against six bacterial strains Bacillus subtillis, Proteus vulgaris, Staphylococus aureus (Gram-positive), Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi (Gram-negative), and fungal species Aspergillus niger and Candida albicans. Phytochemical analysis was also performed using the literature methods. Among the studied medicinal plant extracts against the tested bacterial strain, E. helioscopia showed very promising results against both the gram-positive and gram-negative bacterial and fungal species followed by C. foloisom, A. annua and C. sempervirens which have low activity against the fungal. Relatively low activity was shown by A. calamus. The significant antibacterial activity of active extracts was compared with the standard antimicrobics, piperacillin (100 µg/disc) and gentamicin (10 µg/disc).

RESUMO A Luffa Cilíndrica é um gênero de planta da família das Curcubitáceas, possui um fruto con... more RESUMO A Luffa Cilíndrica é um gênero de planta da família das Curcubitáceas, possui um fruto conhecido como esponja vegetal, que apresenta um arranjo multidirecional de fibras que forma uma manta natural que possui um grande potencial para aplicações em reforço de compósitos de matriz polimérica. Neste trabalho, os frutos da Luffa foram moídos e tratados quimicamente com NaOH ou Metacrilamida em diferentes concentrações e tempos, com o objetivo de caracterizar propriedades físico-químicas e a influência de tratamentos em diferentes condições nestas propriedades, através da determinação da composição química, do índice de cristalinidade, de propriedades térmicas e da morfologia das fibras. Palavras-chave: Luffa Cilíndrica, Tratamentos Químicos, Caracterização INTRODUÇÃO A Luffa Cilíndrica é uma planta da família das Curcubitáceas e dá origem a um fruto conhecido como esponja vegetal. É uma espécie de origem Asiática, sendo cultivada prati camente em todas as regiões do Brasil. Este ...

Orientadora: Thais H.Sydenstricker Flores-Sahagun Co-orientadores: José V.C.Vargas e Sandro C.Ami... more Orientadora: Thais H.Sydenstricker Flores-Sahagun Co-orientadores: José V.C.Vargas e Sandro C.Amico Inclui apęndice Inclui bibliografia Área de concentraçao: Engenharia e ciencias de materiais

International Polymer Processing, 2014

ABSTRACT Considering the growing interest in the development of new materials based on renewable ... more ABSTRACT Considering the growing interest in the development of new materials based on renewable materials, this paper presents the results of experiments carried out with high content (80 to 95 %) of coconut endocarp particles in phenol formalde-hyde resin for the development of composite material by compression molding. The morphology of the particles and chemical composition and thermal stability of both the particles and the resin were evaluated. Tensile and flexural properties of the composites exhibited significant improvements with increasing resin content, attributed to good bonding between the resin and the particles and the presence of fewer voids in the composites as well as to the microstructural homogeneity, as corroborated by the fractographic studies. The thermal stability of the composites is influenced by the proportion of particles , while the swelling characteristics are affected by the amount of resin. The results taken together suggest that the developed composite can be used for various applications.