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Papers by Valeria Garrido

International Journal of Pharmacological Research, 2016

We report the first preclinical tests showing that aminomethylnaphthoquinone - 3-[N-(n-butyl) ami... more We report the first preclinical tests showing that aminomethylnaphthoquinone - 3-[N-(n-butyl) amino-2,4-diclorobenzyl]-2-hydroxy-1,4-naphthoquinone (AMNQ 1) has low cytotoxicity and anti-HSV-1 activity in vitro in Vero cells. The aim of this study was to evaluate the acute toxicity of AMNQ 1 in BALB/c mice. We used BALB/c female mice to evaluate the median lethal dose (LD50) of AMNQ 1 with 2000 mg/kg body weight and other three concentrations using 550, 175 and 55 mg/kg. We compared this to acyclovir (ACV-50 mg/kg) and 10% dimethyl sulfoxide (10% DMSO) over a 14-day period. The BALB/c mice received a single oral dose by gavage. There were no deaths in either group and no change in the murine clinical signs. The hematological and biochemical analyses showed some changes that returned to reference levels without impairment of hemostasis. The AMNQ 1 treatment did not induce untoward changes in organs as shown by histological studies. The in vivo results showed that AMNQ 1 has low toxic...

Journal of Applied Phycology, 2016

Journal of Applied Phycology, 2015

Revista Brasileira de Farmacognosia, 2012

Wistar rats (n=20) were divided in two groups: G1 received 2 mg/kg of GBE (Ginkgo biloba extract ... more Wistar rats (n=20) were divided in two groups: G1 received 2 mg/kg of GBE (Ginkgo biloba extract 761), whereas G2 received the same volume of a sodium chloride solution (0.9%), both for 10 days. After a 7-day interval, the treatment was repeated for 8 days. Urine volume and food and water intake were measured daily during this protocol. Histological assessments were performed. No significant difference (p>0.05) was observed in food and water intake of animals during treatment with GBE. Animals who received GBE had a smaller urine volume and increase of weight with a significance difference (p<0.05) during the first and second exposure period. No histological alteration was observed in tissues, except for the kidney of the experimental group, which revealed a higher concentration of red cells in the glomerulus with a strong staining for Vascular Endothelial Growth Factor (VEGF). The introduction of GBE (therapeutic dose) in health rats may promote alterations in the physiology of the kidney, but no sufficient to modify the glomerulus architecture, including at ultra structural level (electron microscopy).

Revista Brasileira de Farmacognosia, 2011

Dolabelladienotriol is a product extracted from the brown marine alga Dictyota pfaffii from Brazi... more Dolabelladienotriol is a product extracted from the brown marine alga Dictyota pfaffii from Brazil that has been shown to have antiviral activity and low cytotoxicity. Our studies have evaluated the acute toxicity of dolabelladienotriol in BALB/c mice for ten days after administration of a single dose. Among the parameters considered were behavior, weight, biochemical and histological analyses of blood samples taken at three different times (Bs.0, Bs.1 and Bs.2) and optical microscopic examination of organs like liver, kidney, stomach and small intestine. Mice deaths were not observed at any dose during the ten day period. There were some changes in the biochemical analysis results for urea nitrogen (BUN) and alanine aminotransferase (ALT), but the changes were not significantly different from the reference levels of the animals before administration of the substance. Histological analyses of tissues were very similar for all animals. The alterations in liver and kidney tissues did not affect the animals´ behavior at any concentration, not even at 50 mg/kg, where the most significant changes in tissues were seen. This study indicates that dolabelladienotriol has low toxicity in administered dose range.

International Journal of Experimental Pathology, 2009

Inflammatory bowel disease (IBD), like ulcerative colitis, Crohn's disease and others non-infecti... more Inflammatory bowel disease (IBD), like ulcerative colitis, Crohn's disease and others non-infectious inflammation of the gut, have posed an enigma to gastroenterologists and immunologists alike since their first modern description some 75-100 years ago, consisting of one of the major causes of lifetime morbidity (Strober et al. 2007; Weber INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY

Immunobiology, 2009

Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the ... more Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the oral rout are still poorly understood. In our previous studies, we have shown that when normal mice eat peanuts they become tolerant, with no gut alterations. Conversely, if immunized with peanut proteins prior to a challenge diet (CD) containing peanuts they develop chronic inflammation of the gut. Our aim is to evaluate the consequences of the introduction of a novel protein in the diet of animals presenting antigen-specific gut inflammation. Adult, female C57BL/6J mice were divided in control (C) and experimental (E) groups. C1-C3 received peanut protein immunization, animals of the control groups C4 were sham immunized, and control group C5 received ovalbumin (OVA) immunization. The experimental group was immunized with peanut protein extract. Before initial exposure to a 30-day peanut containing CD, the experimental group was divided into 5 groups (E1-E5). OVA feeding began 7 days prior CD (E1) on day 0 (E2), 7 (E3), 14 (E4) and 21 (E5) during CD. Our results show that oral exposure to a novel protein (OVA) in the absence of gut inflammation (E1) leads to low levels of systemic antibody (Ab) titers, comparable to tolerant animals. Conversely, as off initial induction of inflammation, groups submitted to OVA (OT) protocol develop increasingly higher systemic Ab titers similar to animals of the immune control group. In conclusion, our protocol indicates that timing is more important than the antigenicity when a novel protein is offered, in the diet.

Bioorganic & Medicinal Chemistry, 2009

In the present article, we describe the synthesis, anti-HIV1 profile and molecular modeling evalu... more In the present article, we describe the synthesis, anti-HIV1 profile and molecular modeling evaluation of 11 oxoquinoline derivatives. The structure-activity relationship analysis revealed some stereoelectronic properties such as LUMO energy, dipole moment, number of rotatable bonds, and of hydrogen bond donors and acceptors correlated with the potency of compounds. We also describe the importance of substituents R 2 and R 3 for their biological activity. Compound 2j was identified as a lead compound for future investigation due to its : (i) high activity against HIV-1, (ii) low cytotoxicity in PBMC, (iii) low toxic risks based on in silico evaluation, (iv) a good theoretical oral bioavailability according to Lipinski 'rule of five', (v) higher druglikeness and drug-score values than current antivirals AZT and efavirenz.

Current Pharmaceutical Design, Jul 12, 2017

Cardiovascular disease is the leading cause of death worldwide. The heart is susceptible to patho... more Cardiovascular disease is the leading cause of death worldwide. The heart is susceptible to pathologies that impact the myocardium directly, such as myocardial infarction and consequent heart failure, as well as conditions with indirect cardiac effects, such as cancer treatmentrelated cardiotoxicity. As the contractile cells of the heart, cardiomyocytes are essential for