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Papers by Vanessa Pereira Teixeira
CONTRACTIONWAVE (CW) is a free software developed in Python Programming Language that allows the ... more CONTRACTIONWAVE (CW) is a free software developed in Python Programming Language that allows the user to visualize, quantify, and analyze cell contractility parameters in a simple and intuitive format. The software enables the user to acquire membrane kinetics data of cell contractility during contraction-relaxation cycles through image capture and a dense optical flow algorithm. Both method and software were developed using multidisciplinary knowledge, which resulted in a robust data extraction protocol. CONTRACTIONWAVE was originally published in Scalzo et al., Dense optical flow software to quantify cellular contractility, Cell Reports Methods (2021), doi.org/10.1016/j.crmeth.2021.100044. Any use of CW should cite the paper. CW are distributed under the terms of the GNU General Public License version 2, and its source code is available at https://github.com/marceloqla/ContractionWavePy
British Journal of Pharmacology
Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple p... more Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple physiological and pathological processes. A long-held belief is that peptide fragments of BK-(1-9) are biologically inactive. Here, we have tested the biological activities of BK-(1-9) and two major peptide fragments in human and animal systems. Experimental Approach: Levels of BK peptides in male Wistar rat plasma were quantified by mass spectrometric methods. Nitric oxide was quantified in human, mouse and rat cells, and loaded with DAF-FM. We used aortic rings from adult male Wistar rats to test vascular reactivity. Changes in blood pressure and heart rate were measured in conscious adult male Wistar rats. Key results: Plasma levels of BK-(1-7) and BK-(1-5) in rats were increased following infusion of BK-(1-9). All tested peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B1 or B2 receptor antagonists. BK-(1-7) or BK-(1-5) also induced concentration-dependent vasorelaxation of aortic rings, without involving B1 or B2 receptors. In vivo, either intravenous or intra-arterial administration of BK-(1-7) or BK-(1-5) induced similar hypotension response. Conclusions and implications: BK-(1-7) and BK-(1-5) are endogenous peptides present in plasma. They are formed, at least partially, through the BK-(1-9) proteolysis. BK-related peptide fragments show biological activity, not mediated by B1 or B2 receptors. These BK-fragments could constitute new, active components of the kallikrein-kinin system.
Fisioterapia e Pesquisa
RESUMO Este estudo teve por objetivo avaliar o impacto do treinamento físico sobre o desempenho f... more RESUMO Este estudo teve por objetivo avaliar o impacto do treinamento físico sobre o desempenho físico em pacientes com dermatomiosite e polimiosite. Para tanto, uma revisão sistemática e metanálise foi conduzida de acordo com as diretrizes do PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). A pesquisa bibliográfica foi realizada nas seguintes bases de dados: PubMed/MEDLINE e Web of Science, utilizando combinações das seguintes palavras-chave em inglês: dermatomyositis OR polymyositis OR myositis AND exercise OR physical exercise OR physical therapy OR aerobic exercise OR endurance exercise OR resistance exercise. Foram incluídos estudos que atenderam aos seguintes critérios: (1) os participantes apresentavam diagnóstico de dermatomiosite ou polimiosite; (2) os pacientes foram submetidos a um protocolo de treinamento físico; (3) o desempenho físico foi mensurado antes e após o protocolo de treinamento físico. Um total de 14 artigos foram selecionados para...
Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple p... more Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple physiological and pathological processes. A long-held belief is that peptide fragments of BK-(1-9) are biologically inactive. Here, we have tested the biological activities of BK-(1-9) and two major peptide fragments in human and animal systems. Experimental Approach: Levels of BK peptides in male Wistar rat plasma were quantified by mass spectrometric methods. Nitric oxide was quantified in human, mouse and rat cells, and loaded with DAF-FM. We used aortic rings from adult male Wistar rats to test vascular reactivity. Changes in blood pressure and heart rate were measured in conscious adult male Wistar rats. Key results: Plasma levels of BK-(1-7) and BK-(1-5) in rats were increased following infusion of BK-(1-9). All tested peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B1 or B2 recept...
Brazilian Journal of Physical Therapy, 2010
The journal of physiological sciences : JPS, Jan 23, 2015
Obesity increases the risk of respiratory disease, which is associated with airway hyperresponsiv... more Obesity increases the risk of respiratory disease, which is associated with airway hyperresponsiveness. Although the molecular underpinnings of this phenomenon are not well established, lung remodeling is known as an important factor in this process and could potentially explain compromised lung functions. In the present study, the obesity was induced by postnatal overnutrition in Swiss mice and we investigated the pulmonary mechanics after aerosolization of saline, methacholine, and salbutamol. The lungs were prepared for morphometric analysis. Obese animals showed bronchoconstriction in response to methacholine, as evidenced by airway and tissue resistance, tissue elastance, and hysteresivity. Salbutamol was effective at recovering the response only for airway resistance but not for tissue mechanics. We suggest that this impaired response in obese mice is related to collapsed alveolar, to inflammatory cells, and to elevated deposition collagen fibers in parenchymal tissue.
Introduction: The waist-hip ratio (WHR) is one of the variables used for measurements of body. Th... more Introduction: The waist-hip ratio (WHR) is one of the variables used for measurements of body. The Pilates method is considered a type of exercise that demonstrates positive results to several systems, however, higher adherence to this activity refers to the loss of body measurements and the literature contains few studies on these variables. Objective: To evaluate the effect of 12-week training in Pilates MAT reduction in waist and hip measurements in young sedentary. Method: Fifteen young sedentary (9 women and 6 men) were selected to participate in the training program. The WHR was assessed before and after the training period. Training with Pilates MAT had a frequency of 2 sessions per week for a period of 12 weeks. The sessions had a total duration of 60 minutes. Each session consisted of the following phases: Phase 1 (heating and stretching), phase 2 (Pilates MAT) and phase 3 (slowdown). Results: The study sample was initially composed of 18 volunteers, but only 15 of these vo...
Ter. man, 2009
Base de dados : LILACS. Pesquisa : 538008 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 538008 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 538008. ...
Respiratory Physiology & Neurobiology, 2014
Bronchial responsiveness during methacholine (MCh) challenge was analysed in an elastase-induced ... more Bronchial responsiveness during methacholine (MCh) challenge was analysed in an elastase-induced mouse model of emphysema to explore the magnitude of the response in this model. Swiss mice were intratracheally instilled with saline or elastase (0.3 or 0.6 U). Twenty days afterward, mechanical ventilation data were collected from the closed and opened thorax of baseline and MCh (vehicle, 50 and 100 mg/mL) challenged mice. The lungs were prepared for morphometric analysis. In the 0.6 U group, airway resistance (R aw) and tissue elastance (H) were decreased, and hysteresivity (Á) was increased (closed thorax). MCh increased R aw , G and H in all groups, but this increase was attenuated in the elastase-induced emphysema groups, the largest attenuation was observed in the 0.6 U (closed thorax condition). Elastase increased hyperinflation of the alveoli, alveolar collapse and the Lm and reduced the normal area. MCh reduced respiratory mechanics in elastase-induced emphysema, and this reduction was modulated by the collapsed and/or hyperinflated areas, which increased the heterogeneity of the lungs.
Journal of Bodywork and Movement Therapies, 2013
Background and purpose: Bradykinin [BK-(1-9)] is an endogenous peptide involved in many physiolog... more Background and purpose: Bradykinin [BK-(1-9)] is an endogenous peptide involved in many physiological and pathological processes, such as cardiovascular homeostasis and inflammation. The central dogma of the kallikrein-kinin system is that BK-(1-9) fragments are biologically inactive. In this manuscript, we proposed to test whether these fragments were indeed inactive. Experimental Approach: Nitric oxide (NO) was quantified in human, mouse and rat cells loaded with DAF-FM after stimulation with BK-(1-9), BK-(1-7), BK-(1-5) and BK-(1-3). We used adult male rat aortic ring preparation to test vascular reactivity mediated by BK-(1-9) fragments. Changes in blood pressure and heart rate was measured in conscious adult male rats by intraarterial catheter method. Key results: BK-(1-9) induced NO production in all cell types tested by B2 receptor activation. BK-(1-7), BK-(1-5) and BK-(1-3) also induced NO production in all tested cell types but this response was independent of the activatio...
CONTRACTIONWAVE (CW) is a free software developed in Python Programming Language that allows the ... more CONTRACTIONWAVE (CW) is a free software developed in Python Programming Language that allows the user to visualize, quantify, and analyze cell contractility parameters in a simple and intuitive format. The software enables the user to acquire membrane kinetics data of cell contractility during contraction-relaxation cycles through image capture and a dense optical flow algorithm. Both method and software were developed using multidisciplinary knowledge, which resulted in a robust data extraction protocol. CONTRACTIONWAVE was originally published in Scalzo et al., Dense optical flow software to quantify cellular contractility, Cell Reports Methods (2021), doi.org/10.1016/j.crmeth.2021.100044. Any use of CW should cite the paper. CW are distributed under the terms of the GNU General Public License version 2, and its source code is available at https://github.com/marceloqla/ContractionWavePy
British Journal of Pharmacology
Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple p... more Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple physiological and pathological processes. A long-held belief is that peptide fragments of BK-(1-9) are biologically inactive. Here, we have tested the biological activities of BK-(1-9) and two major peptide fragments in human and animal systems. Experimental Approach: Levels of BK peptides in male Wistar rat plasma were quantified by mass spectrometric methods. Nitric oxide was quantified in human, mouse and rat cells, and loaded with DAF-FM. We used aortic rings from adult male Wistar rats to test vascular reactivity. Changes in blood pressure and heart rate were measured in conscious adult male Wistar rats. Key results: Plasma levels of BK-(1-7) and BK-(1-5) in rats were increased following infusion of BK-(1-9). All tested peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B1 or B2 receptor antagonists. BK-(1-7) or BK-(1-5) also induced concentration-dependent vasorelaxation of aortic rings, without involving B1 or B2 receptors. In vivo, either intravenous or intra-arterial administration of BK-(1-7) or BK-(1-5) induced similar hypotension response. Conclusions and implications: BK-(1-7) and BK-(1-5) are endogenous peptides present in plasma. They are formed, at least partially, through the BK-(1-9) proteolysis. BK-related peptide fragments show biological activity, not mediated by B1 or B2 receptors. These BK-fragments could constitute new, active components of the kallikrein-kinin system.
Fisioterapia e Pesquisa
RESUMO Este estudo teve por objetivo avaliar o impacto do treinamento físico sobre o desempenho f... more RESUMO Este estudo teve por objetivo avaliar o impacto do treinamento físico sobre o desempenho físico em pacientes com dermatomiosite e polimiosite. Para tanto, uma revisão sistemática e metanálise foi conduzida de acordo com as diretrizes do PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). A pesquisa bibliográfica foi realizada nas seguintes bases de dados: PubMed/MEDLINE e Web of Science, utilizando combinações das seguintes palavras-chave em inglês: dermatomyositis OR polymyositis OR myositis AND exercise OR physical exercise OR physical therapy OR aerobic exercise OR endurance exercise OR resistance exercise. Foram incluídos estudos que atenderam aos seguintes critérios: (1) os participantes apresentavam diagnóstico de dermatomiosite ou polimiosite; (2) os pacientes foram submetidos a um protocolo de treinamento físico; (3) o desempenho físico foi mensurado antes e após o protocolo de treinamento físico. Um total de 14 artigos foram selecionados para...
Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple p... more Background and purpose: Bradykinin [BK-(1-9)] is an endogenous nonapeptide involved in multiple physiological and pathological processes. A long-held belief is that peptide fragments of BK-(1-9) are biologically inactive. Here, we have tested the biological activities of BK-(1-9) and two major peptide fragments in human and animal systems. Experimental Approach: Levels of BK peptides in male Wistar rat plasma were quantified by mass spectrometric methods. Nitric oxide was quantified in human, mouse and rat cells, and loaded with DAF-FM. We used aortic rings from adult male Wistar rats to test vascular reactivity. Changes in blood pressure and heart rate were measured in conscious adult male Wistar rats. Key results: Plasma levels of BK-(1-7) and BK-(1-5) in rats were increased following infusion of BK-(1-9). All tested peptides induced NO production in all cell types tested. However, unlike BK-(1-9), NO production elicited by BK-(1-7) or BK-(1-5) was not inhibited by B1 or B2 recept...
Brazilian Journal of Physical Therapy, 2010
The journal of physiological sciences : JPS, Jan 23, 2015
Obesity increases the risk of respiratory disease, which is associated with airway hyperresponsiv... more Obesity increases the risk of respiratory disease, which is associated with airway hyperresponsiveness. Although the molecular underpinnings of this phenomenon are not well established, lung remodeling is known as an important factor in this process and could potentially explain compromised lung functions. In the present study, the obesity was induced by postnatal overnutrition in Swiss mice and we investigated the pulmonary mechanics after aerosolization of saline, methacholine, and salbutamol. The lungs were prepared for morphometric analysis. Obese animals showed bronchoconstriction in response to methacholine, as evidenced by airway and tissue resistance, tissue elastance, and hysteresivity. Salbutamol was effective at recovering the response only for airway resistance but not for tissue mechanics. We suggest that this impaired response in obese mice is related to collapsed alveolar, to inflammatory cells, and to elevated deposition collagen fibers in parenchymal tissue.
Introduction: The waist-hip ratio (WHR) is one of the variables used for measurements of body. Th... more Introduction: The waist-hip ratio (WHR) is one of the variables used for measurements of body. The Pilates method is considered a type of exercise that demonstrates positive results to several systems, however, higher adherence to this activity refers to the loss of body measurements and the literature contains few studies on these variables. Objective: To evaluate the effect of 12-week training in Pilates MAT reduction in waist and hip measurements in young sedentary. Method: Fifteen young sedentary (9 women and 6 men) were selected to participate in the training program. The WHR was assessed before and after the training period. Training with Pilates MAT had a frequency of 2 sessions per week for a period of 12 weeks. The sessions had a total duration of 60 minutes. Each session consisted of the following phases: Phase 1 (heating and stretching), phase 2 (Pilates MAT) and phase 3 (slowdown). Results: The study sample was initially composed of 18 volunteers, but only 15 of these vo...
Ter. man, 2009
Base de dados : LILACS. Pesquisa : 538008 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 538008 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 538008. ...
Respiratory Physiology & Neurobiology, 2014
Bronchial responsiveness during methacholine (MCh) challenge was analysed in an elastase-induced ... more Bronchial responsiveness during methacholine (MCh) challenge was analysed in an elastase-induced mouse model of emphysema to explore the magnitude of the response in this model. Swiss mice were intratracheally instilled with saline or elastase (0.3 or 0.6 U). Twenty days afterward, mechanical ventilation data were collected from the closed and opened thorax of baseline and MCh (vehicle, 50 and 100 mg/mL) challenged mice. The lungs were prepared for morphometric analysis. In the 0.6 U group, airway resistance (R aw) and tissue elastance (H) were decreased, and hysteresivity (Á) was increased (closed thorax). MCh increased R aw , G and H in all groups, but this increase was attenuated in the elastase-induced emphysema groups, the largest attenuation was observed in the 0.6 U (closed thorax condition). Elastase increased hyperinflation of the alveoli, alveolar collapse and the Lm and reduced the normal area. MCh reduced respiratory mechanics in elastase-induced emphysema, and this reduction was modulated by the collapsed and/or hyperinflated areas, which increased the heterogeneity of the lungs.
Journal of Bodywork and Movement Therapies, 2013
Background and purpose: Bradykinin [BK-(1-9)] is an endogenous peptide involved in many physiolog... more Background and purpose: Bradykinin [BK-(1-9)] is an endogenous peptide involved in many physiological and pathological processes, such as cardiovascular homeostasis and inflammation. The central dogma of the kallikrein-kinin system is that BK-(1-9) fragments are biologically inactive. In this manuscript, we proposed to test whether these fragments were indeed inactive. Experimental Approach: Nitric oxide (NO) was quantified in human, mouse and rat cells loaded with DAF-FM after stimulation with BK-(1-9), BK-(1-7), BK-(1-5) and BK-(1-3). We used adult male rat aortic ring preparation to test vascular reactivity mediated by BK-(1-9) fragments. Changes in blood pressure and heart rate was measured in conscious adult male rats by intraarterial catheter method. Key results: BK-(1-9) induced NO production in all cell types tested by B2 receptor activation. BK-(1-7), BK-(1-5) and BK-(1-3) also induced NO production in all tested cell types but this response was independent of the activatio...