Varsha Shahane - Academia.edu (original) (raw)
Papers by Varsha Shahane
... Dr. DY Patil Medical College, Pune. Nageswari R. Gandham*, Rabindra Nath Misra**, Gurbux B. M... more ... Dr. DY Patil Medical College, Pune. Nageswari R. Gandham*, Rabindra Nath Misra**, Gurbux B. Matnani***, Varsha D. Shahane*, Savita V. Jadhav****, Mahadev T. Juagare**** ... J. of Medical Microbiology, 1998; 16 (4) : 152-3 6. Savitri Sharma, Prashant Garg, Gullapalli Rav. ...
Trends in Biosciences, 2013
Novel polyenzyme formulations which are proprietary blends of different enzymes such as bromelain... more Novel polyenzyme formulations which are proprietary blends of different enzymes such as bromelain, papain, acid and alkaline proteases, serratiopeptidase, lysozyme etc. have been developed by Advanced Enzyme Technologies Ltd., Thane and Specialty Enzymes Co. USA, group of Companies, Screening for antibacterial activity of Immunoseb, a novel polyenzyme formulation under development, was done using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, Streptococcus pyogenes and S. pneumoniae employing a modified antibiotic susceptibility test. The polyenzyme formulation was found to have activity against P. aeruginosa S. aureus, S. pyogenes and S. pneumoniae at the test concentration. Synergistic effect was established when Immunoseb was used in conjunction with Ampicillin against E. coli and with Penicillin against Streptococcus pyogenes. Partial synergy was established when it was used in conjunction with Ceftazidime against ...
This is a report of an outbreak of cholera occurred during August-October 2005 following heavy ra... more This is a report of an outbreak of cholera occurred during August-October 2005 following heavy rainfall in Pune district, Microbiological investigations revealed a total of 31 Vibrio cholerae strains from 101 suspected cholera cases. Of the 31 strains isolated, 28 were O1 EI Tor Ogawa vibrios, while three were NAG (non-O1 agglutinable) vibrios. T4 phage type was found to be prevalent in Pune area. Furazolidone resistance in vibrio isolates has been recorded in the present outbreak. Key words: Cholera, Vibrio cholerae O1 EI Tor Ogawa, Furazolidone resistance
Routine culture & sensitivity reports are available only after 48hrs, forcing clinicians to resor... more Routine culture & sensitivity reports are available only after 48hrs, forcing clinicians to resort to empiricism. The present study was undertaken with an aim to hasten antimicrobial susceptibility reporting with existing infrastructure. Hundred isolates (gram positive and gram negative) from various clinical samples were subjected in duplicate to antimicrobial susceptibility testing by a new, simple method and compared with the routine method. Comparable results were obtained by the new method within 6hrs, cutting down the reporting time by 24hrs. Early reporting time should encourage clinicians to postpone antimicrobial treatment in non-life threatening situations, thereby reducing the indiscriminate use of antibiotics. Key words- Antimicrobial sensitivity testing, early.
Extra-pulmonary tuberculosis often poses a diagnostic dilemma for the treating clinician. A confi... more Extra-pulmonary tuberculosis often poses a diagnostic dilemma for the treating clinician. A confirmed laboratory report regarding the presence of acid fast bacteria, will help overcome the problem. The present study was a retrospective analysis of the role of our mycobacteriology laboratory in aiding the clinicians in reaching a diagnosis. The importance of culture vis-a-vis samples were received for mycobacterial culture, 125 (72%) of these samples were from extra-pulmonary sites. Ten out of 125 (8%) samples were smear positive and 26/125 i.e. 20.8% were culture positive. Six samples (5%) were smear and culture positive, 4 (3%) were smear positive but culture negative and 20 (16%) were smear negative and culture positive. Our results emphasize the importance of culture over microscopy. Key words: extra-pulmonary tuberculosis, microscopy, culture
The Indian Journal of Medical Research
The Bombay Hospital journal
Vector borne diseases and faeco-orally transmitted infections are major health problems in develo... more Vector borne diseases and faeco-orally transmitted infections are major health problems in developing countries. We report here a case of migrant from Bihar who presented with fever and myalgia. He was diagnosed as a case of pyrexia of unknown origin. His Dengue IgM ELISA was positive and routine stool microscopy revealed eggs of Trichuris trichura, Ascaris lumbricoides and Ankylostoma duodenale. He responded to mebendazole and supportive therapy and was discharged within five days of admission after an uneventful stay in the hospital. Multiple helminthiasis is very rarely reported in Maharashtra. The helminthic infection and Dengue in this case is purely co-incidental.
Cutaneous infections constitute a major portion of patients in any dermatology clinic. They pose ... more Cutaneous infections constitute a major portion of patients in any dermatology clinic. They pose a therapeutic dilemma and many patients do not respond to commonly used antibiotics. Perhaps indiscriminate use of topical and systemic antibiotics have contributed to this situation. A bacteriological study was done in 50 clinically diagnosed cases of pyoderma. Of these, 27 were of primary pyoderma and 23 cases of secondary pyoderma. Among primary pyoderma, impetigo (40.7%), Folliculitis (25.9%) and Cellulitis (14.8%) were the common clinical presentations while in secondary pyoderma, Scabies (43.5%) and Eczema (34.5%) were the most common isolates followed by Gram negative isolates such as Klebsiella pneumoniae (6.8%), Pseudomonas aerogenosa, Cirtobacter fruenoli, and E-coli (5.08% each). Gram positive isolates showed maximum sensitivity to Amikacin (84.6%), Azythromycin (84.8%) and Ciprofloxacin (78.3%), while gram-negative isolates were sensitive to Amikacin and Ciprofloxacin (76.9%)...
Septicemia is a major cause of morbidity and mortality in neonates. Over a one year period blood ... more Septicemia is a major cause of morbidity and mortality in neonates. Over a one year period blood culture was performed on 200 neonates admitted to the NICU of Sassoon Hospital with a clinical suspicion of septicemia. Culture was positive in 114 (57%) of the babies. Gram negative organisms were obtained in 90 (78.8%) neonates. Klebsiella pneumoniae was the single commonest pathogen in 59 (51.7%) babies. Gram negative organisms were obtained in 24 (21%) neonates. Environmental samples from NICU revealed Klebsiella spp on culture on two occasions, indicating that sepsis could be of nosocomial origin. Antibiotic sensitivity of Klebsiella isolates showed maximum sensitivity for Amikacin (67%). Mortality in those babies was 48.2%. Appropriate and early therapy could improve the progress in this condition. Key words: Neonatal septicemia, Klebsiella pneumoniae, Nosocomial transmission
Hindustan antibiotics bulletin
A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Sp... more A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Speciality Biochemicals Co., USA, was tested for antibacterial properties using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumoniae. A modified antibiotic susceptibility test was used for the purpose. S. aureus, S. pyogenes and S. pneumoniae were found to be sensitive to the formulation at the chosen concentration. Next, to study the interaction between Gumseb and currently used antibiotic, a checkerboard Minimum Inhibitory Concentrations (MIC) was carried out for each organism. The assay was carried out with the aim of establishing whether the polyenzyme formulation had any potentiating effect on the antibiotic of choice. Synergistic effect was established when Gumseb was used in conjunction with penicillin against S. pyogenes. Partial synergy was established when it wa...
Hindustan Antibiotics Bulletin, 2005
A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Sp... more A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Speciality Biochemicals Co., USA, was tested for antibacterial properties using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumoniae. A modified antibiotic susceptibility test was used for the purpose. S. aureus, S. pyogenes and S. pneumoniae were found to be sensitive to the formulation at the chosen concentration. Next, to study the interaction between Gumseb and currently used antibiotic, a checkerboard Minimum Inhibitory Concentrations (MIC) was carried out for each organism. The assay was carried out with the aim of establishing whether the polyenzyme formulation had any potentiating effect on the antibiotic of choice. Synergistic effect was established when Gumseb was used in conjunction with penicillin against S. pyogenes. Partial synergy was established when it was used in conduction with Ceftazidime against P. aeruginosa and with Ciprofloxacin against methicillin sensitive, coagulase positive S. aureus. Antagonism was established when it was used in conjunction with Ampicillin against E. coli, with Ciprofloxacin against S. typhi and coagulase negative staphylococcal strain. The results indicate that Gumseb can be used in conjunction with those antibiotics with which a synergistic or a partially synergistic effect could be shown, as in the case of P. aerugionosa and S. aureus. These findings have particular importance since these organisms are responsible for hospital based infections and are notorious for antibiotic resistance. In cases where antagonism was established, it should not be used in combination with that particular antibiotic. However, it can be used individually since it has proved to have antibacterial activity and MIC could be determined for all five commonly encountered pathogens. Therefore, it has the potential of being a novel broad range antibacterial drug. These findings are significant given the alarming rise in incidence of antibiotic resistance in most clinically important pathogens.
Hindustan antibiotics bulletin
A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Sp... more A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Speciality Biochemicals Co., USA, was tested for antibacterial properties using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumoniae. A modified antibiotic susceptibility test was used for the purpose. S. aureus, S. pyogenes and S. pneumoniae were found to be sensitive to the formulation at the chosen concentration. Next, to study the interaction between Gumseb and currently used antibiotic, a checkerboard Minimum Inhibitory Concentrations (MIC) was carried out for each organism. The assay was carried out with the aim of establishing whether the polyenzyme formulation had any potentiating effect on the antibiotic of choice. Synergistic effect was established when Gumseb was used in conjunction with penicillin against S. pyogenes. Partial synergy was established when it wa...
... Dr. DY Patil Medical College, Pune. Nageswari R. Gandham*, Rabindra Nath Misra**, Gurbux B. M... more ... Dr. DY Patil Medical College, Pune. Nageswari R. Gandham*, Rabindra Nath Misra**, Gurbux B. Matnani***, Varsha D. Shahane*, Savita V. Jadhav****, Mahadev T. Juagare**** ... J. of Medical Microbiology, 1998; 16 (4) : 152-3 6. Savitri Sharma, Prashant Garg, Gullapalli Rav. ...
Trends in Biosciences, 2013
Novel polyenzyme formulations which are proprietary blends of different enzymes such as bromelain... more Novel polyenzyme formulations which are proprietary blends of different enzymes such as bromelain, papain, acid and alkaline proteases, serratiopeptidase, lysozyme etc. have been developed by Advanced Enzyme Technologies Ltd., Thane and Specialty Enzymes Co. USA, group of Companies, Screening for antibacterial activity of Immunoseb, a novel polyenzyme formulation under development, was done using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, Streptococcus pyogenes and S. pneumoniae employing a modified antibiotic susceptibility test. The polyenzyme formulation was found to have activity against P. aeruginosa S. aureus, S. pyogenes and S. pneumoniae at the test concentration. Synergistic effect was established when Immunoseb was used in conjunction with Ampicillin against E. coli and with Penicillin against Streptococcus pyogenes. Partial synergy was established when it was used in conjunction with Ceftazidime against ...
This is a report of an outbreak of cholera occurred during August-October 2005 following heavy ra... more This is a report of an outbreak of cholera occurred during August-October 2005 following heavy rainfall in Pune district, Microbiological investigations revealed a total of 31 Vibrio cholerae strains from 101 suspected cholera cases. Of the 31 strains isolated, 28 were O1 EI Tor Ogawa vibrios, while three were NAG (non-O1 agglutinable) vibrios. T4 phage type was found to be prevalent in Pune area. Furazolidone resistance in vibrio isolates has been recorded in the present outbreak. Key words: Cholera, Vibrio cholerae O1 EI Tor Ogawa, Furazolidone resistance
Routine culture & sensitivity reports are available only after 48hrs, forcing clinicians to resor... more Routine culture & sensitivity reports are available only after 48hrs, forcing clinicians to resort to empiricism. The present study was undertaken with an aim to hasten antimicrobial susceptibility reporting with existing infrastructure. Hundred isolates (gram positive and gram negative) from various clinical samples were subjected in duplicate to antimicrobial susceptibility testing by a new, simple method and compared with the routine method. Comparable results were obtained by the new method within 6hrs, cutting down the reporting time by 24hrs. Early reporting time should encourage clinicians to postpone antimicrobial treatment in non-life threatening situations, thereby reducing the indiscriminate use of antibiotics. Key words- Antimicrobial sensitivity testing, early.
Extra-pulmonary tuberculosis often poses a diagnostic dilemma for the treating clinician. A confi... more Extra-pulmonary tuberculosis often poses a diagnostic dilemma for the treating clinician. A confirmed laboratory report regarding the presence of acid fast bacteria, will help overcome the problem. The present study was a retrospective analysis of the role of our mycobacteriology laboratory in aiding the clinicians in reaching a diagnosis. The importance of culture vis-a-vis samples were received for mycobacterial culture, 125 (72%) of these samples were from extra-pulmonary sites. Ten out of 125 (8%) samples were smear positive and 26/125 i.e. 20.8% were culture positive. Six samples (5%) were smear and culture positive, 4 (3%) were smear positive but culture negative and 20 (16%) were smear negative and culture positive. Our results emphasize the importance of culture over microscopy. Key words: extra-pulmonary tuberculosis, microscopy, culture
The Indian Journal of Medical Research
The Bombay Hospital journal
Vector borne diseases and faeco-orally transmitted infections are major health problems in develo... more Vector borne diseases and faeco-orally transmitted infections are major health problems in developing countries. We report here a case of migrant from Bihar who presented with fever and myalgia. He was diagnosed as a case of pyrexia of unknown origin. His Dengue IgM ELISA was positive and routine stool microscopy revealed eggs of Trichuris trichura, Ascaris lumbricoides and Ankylostoma duodenale. He responded to mebendazole and supportive therapy and was discharged within five days of admission after an uneventful stay in the hospital. Multiple helminthiasis is very rarely reported in Maharashtra. The helminthic infection and Dengue in this case is purely co-incidental.
Cutaneous infections constitute a major portion of patients in any dermatology clinic. They pose ... more Cutaneous infections constitute a major portion of patients in any dermatology clinic. They pose a therapeutic dilemma and many patients do not respond to commonly used antibiotics. Perhaps indiscriminate use of topical and systemic antibiotics have contributed to this situation. A bacteriological study was done in 50 clinically diagnosed cases of pyoderma. Of these, 27 were of primary pyoderma and 23 cases of secondary pyoderma. Among primary pyoderma, impetigo (40.7%), Folliculitis (25.9%) and Cellulitis (14.8%) were the common clinical presentations while in secondary pyoderma, Scabies (43.5%) and Eczema (34.5%) were the most common isolates followed by Gram negative isolates such as Klebsiella pneumoniae (6.8%), Pseudomonas aerogenosa, Cirtobacter fruenoli, and E-coli (5.08% each). Gram positive isolates showed maximum sensitivity to Amikacin (84.6%), Azythromycin (84.8%) and Ciprofloxacin (78.3%), while gram-negative isolates were sensitive to Amikacin and Ciprofloxacin (76.9%)...
Septicemia is a major cause of morbidity and mortality in neonates. Over a one year period blood ... more Septicemia is a major cause of morbidity and mortality in neonates. Over a one year period blood culture was performed on 200 neonates admitted to the NICU of Sassoon Hospital with a clinical suspicion of septicemia. Culture was positive in 114 (57%) of the babies. Gram negative organisms were obtained in 90 (78.8%) neonates. Klebsiella pneumoniae was the single commonest pathogen in 59 (51.7%) babies. Gram negative organisms were obtained in 24 (21%) neonates. Environmental samples from NICU revealed Klebsiella spp on culture on two occasions, indicating that sepsis could be of nosocomial origin. Antibiotic sensitivity of Klebsiella isolates showed maximum sensitivity for Amikacin (67%). Mortality in those babies was 48.2%. Appropriate and early therapy could improve the progress in this condition. Key words: Neonatal septicemia, Klebsiella pneumoniae, Nosocomial transmission
Hindustan antibiotics bulletin
A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Sp... more A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Speciality Biochemicals Co., USA, was tested for antibacterial properties using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumoniae. A modified antibiotic susceptibility test was used for the purpose. S. aureus, S. pyogenes and S. pneumoniae were found to be sensitive to the formulation at the chosen concentration. Next, to study the interaction between Gumseb and currently used antibiotic, a checkerboard Minimum Inhibitory Concentrations (MIC) was carried out for each organism. The assay was carried out with the aim of establishing whether the polyenzyme formulation had any potentiating effect on the antibiotic of choice. Synergistic effect was established when Gumseb was used in conjunction with penicillin against S. pyogenes. Partial synergy was established when it wa...
Hindustan Antibiotics Bulletin, 2005
A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Sp... more A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Speciality Biochemicals Co., USA, was tested for antibacterial properties using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumoniae. A modified antibiotic susceptibility test was used for the purpose. S. aureus, S. pyogenes and S. pneumoniae were found to be sensitive to the formulation at the chosen concentration. Next, to study the interaction between Gumseb and currently used antibiotic, a checkerboard Minimum Inhibitory Concentrations (MIC) was carried out for each organism. The assay was carried out with the aim of establishing whether the polyenzyme formulation had any potentiating effect on the antibiotic of choice. Synergistic effect was established when Gumseb was used in conjunction with penicillin against S. pyogenes. Partial synergy was established when it was used in conduction with Ceftazidime against P. aeruginosa and with Ciprofloxacin against methicillin sensitive, coagulase positive S. aureus. Antagonism was established when it was used in conjunction with Ampicillin against E. coli, with Ciprofloxacin against S. typhi and coagulase negative staphylococcal strain. The results indicate that Gumseb can be used in conjunction with those antibiotics with which a synergistic or a partially synergistic effect could be shown, as in the case of P. aerugionosa and S. aureus. These findings have particular importance since these organisms are responsible for hospital based infections and are notorious for antibiotic resistance. In cases where antagonism was established, it should not be used in combination with that particular antibiotic. However, it can be used individually since it has proved to have antibacterial activity and MIC could be determined for all five commonly encountered pathogens. Therefore, it has the potential of being a novel broad range antibacterial drug. These findings are significant given the alarming rise in incidence of antibiotic resistance in most clinically important pathogens.
Hindustan antibiotics bulletin
A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Sp... more A novel polyenzyme formulation Gumseb developed by Advanced Enzyme Technologies Ltd, Thane and Speciality Biochemicals Co., USA, was tested for antibacterial properties using ATCC strains and clinical isolates of Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumoniae. A modified antibiotic susceptibility test was used for the purpose. S. aureus, S. pyogenes and S. pneumoniae were found to be sensitive to the formulation at the chosen concentration. Next, to study the interaction between Gumseb and currently used antibiotic, a checkerboard Minimum Inhibitory Concentrations (MIC) was carried out for each organism. The assay was carried out with the aim of establishing whether the polyenzyme formulation had any potentiating effect on the antibiotic of choice. Synergistic effect was established when Gumseb was used in conjunction with penicillin against S. pyogenes. Partial synergy was established when it wa...