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Papers by Ariana Vasconcelos

Research paper thumbnail of Toward the mechanism of jarastatin (rJast) inhibition of the integrin αVβ3

International Journal of Biological Macromolecules, Dec 31, 2023

Research paper thumbnail of Galactosaminoglycans: Medical Applications and Drawbacks

Research paper thumbnail of The Sea as a Rich Source of Structurally Unique Glycosaminoglycans and Mimetics

Microorganisms, Aug 28, 2017

Research paper thumbnail of Chemical composition determination and evaluation of the antibacterial activity of essential oils from Ruellia asperula (Mart. Ex Ness) Lindau and Ruellia paniculata L. against oral streptococci

Natural Product Research

This study investigated the chemical composition and evaluated the antibacterial and antibiofilm ... more This study investigated the chemical composition and evaluated the antibacterial and antibiofilm activities of essential oils (EOs) extracted from Ruellia asperula (EORA) and Ruellia paniculata (EORP) against oral streptococci. The EO constituents were analyzed by gas chromatography/mass spectrometry. The antimicrobial potential of EOs was evaluated using the minimum inhibitory concentration, minimum bactericidal concentration, and time-kill determination. Furthermore, the quantification of total biomass and the number of viable cells in the biofilms were evaluated. The major constituents of EORA were cariophylla-4(12)-8-(13)-dien-5β-ol (14.1%), (β)-caryophyllene (22.7%), and caryophyllene oxide (29.4%). For EORP, the major constituents were (β)-caryophyllene (11.0%), spathulenol (13.1%), and δ-amorphene (14.9%). The tested EOs exhibited antibacterial activity against planktonic growth and biofilm formation. Thus, the EOs from R. asperula and R. paniculata prove to be promising alternatives for bacterial growth control and biofilm formation prevention of oral streptococci.

Research paper thumbnail of Leveraging Genome Sequencing Strategies for Basic and Applied Algal Research, Exemplified by Case Studies

Handbook of Algal Technologies and Phytochemicals, 2019

Research paper thumbnail of Structure-Function Relationship of the Disintegrin Family: Sequence Signature and Integrin Interaction

Frontiers in Molecular Biosciences, 2021

Disintegrins are small cysteine-rich proteins found in a variety of snake venom. These proteins s... more Disintegrins are small cysteine-rich proteins found in a variety of snake venom. These proteins selectively modulate integrin function, heterodimeric receptors involved in cell-cell and cell-matrix interaction that are widely studied as therapeutic targets. Snake venom disintegrins emerged from the snake venom metalloproteinase and are classified according to the sequence size and number of disulfide bonds. Evolutive structure and function diversification of disintegrin family involves a stepwise decrease in the polypeptide chain, loss of cysteine residues, and selectivity. Since the structure elucidation of echistatin, the description of the structural properties of disintegrins has allowed the investigation of the mechanisms involved in integrin-cell-extracellular matrix interaction. This review provides an analysis of the structures of all family groups enabling the description of an expanded classification of the disintegrin family in seven groups. Each group presents a particul...

Research paper thumbnail of Biocompatibility and structural characterization of glycosaminoglycans isolated from heads of silver-banded whiting (Sillago argentifasciata Martin & Montalban 1935)

International Journal of Biological Macromolecules, 2020

Research paper thumbnail of Galactosaminoglycans: Medical Applications and Drawbacks

Molecules, 2019

Galactosaminoglycans (GalAGs) are sulfated glycans composed of alternating N-acetylgalactosamine ... more Galactosaminoglycans (GalAGs) are sulfated glycans composed of alternating N-acetylgalactosamine and uronic acid units. Uronic acid epimerization, sulfation patterns and fucosylation are modifications observed on these molecules. GalAGs have been extensively studied and exploited because of their multiple biomedical functions. Chondroitin sulfates (CSs), the main representative family of GalAGs, have been used in alternative therapy of joint pain/inflammation and osteoarthritis. The relatively novel fucosylated chondroitin sulfate (FCS), commonly found in sea cucumbers, has been screened in multiple systems in addition to its widely studied anticoagulant action. Biomedical properties of GalAGs are directly dependent on the sugar composition, presence or lack of fucose branches, as well as sulfation patterns. Although research interest in GalAGs has increased considerably over the three last decades, perhaps motivated by the parallel progress of glycomics, serious questions concernin...

Research paper thumbnail of Marine Carbohydrate-Based Compounds with Medicinal Properties

The oceans harbor a great diversity of organisms, and have been recognized as an important source... more The oceans harbor a great diversity of organisms, and have been recognized as an important source of new compounds with nutritional and therapeutic potential. Among these compounds, carbohydrate-based compounds are of particular interest because they exhibit numerous biological functions associated with their chemical diversity. This gives rise to new substances for the development of bioactive products. Many are the known applications of substances with glycosidic domains obtained from marine species. This review covers the structural properties and the current findings on the antioxidant, anti-inflammatory, anticoagulant, antitumor and antimicrobial activities of medium and high molecular-weight carbohydrates or glycosylated compounds extracted from various marine organisms.

Research paper thumbnail of The Sea as a Rich Source of Structurally Unique Glycosaminoglycans and Mimetics

Research paper thumbnail of Antimicrobial Effect of the Triterpene 3β,6β,16β-Trihydroxylup-20(29)-ene on Planktonic Cells and Biofilms from Gram Positive and Gram Negative Bacteria

BioMed Research International, 2014

This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a tri... more This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a triterpene isolated fromCombretum leprosumMart., in inhibiting the planktonic growth and biofilms of Gram positive bacteriaStreptococcus mutansandS. mitis. The antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibiofilm potential was determined by quantifying total biomass and enumerating biofilm-entrapped viable bacteria. In addition, the acute toxicity of CLF1 onArtemiasp. nauplii was also determined. The results showed that CLF1 was able in inhibiting the growth ofS. mutansandS. mitiswith MIC and MBC of 7.8 μg/mL and 15.6 μg/mL, respectively. CLF1 was highly effective on biofilms of both bacteria. Only 7.8 μg/mL CLF1 was enough to inhibit by 97% and 90% biomass production ofS. mutansandS. mitis, respectively. On the other hand, such effects were not evident on Gram negativePseudomonas aerugino...

Research paper thumbnail of 15N, 13C, and 1H resonance assignments of Jarastatin: a disintegrin of Bothrops jararaca

Biomolecular NMR Assignments

Disintegrins are a group of cysteine-rich proteins found in a wide variety of snake venoms. These... more Disintegrins are a group of cysteine-rich proteins found in a wide variety of snake venoms. These proteins selectively bind to integrins, which play a fundamental role in the regulation of many physiological and pathological processes. Here, we report the NMR chemical shift assignments for 1H, 15N, and 13C nuclei in the backbone and side chains of recombinant disintegrin Jarastatin (rJast), which was further validated by secondary structure prediction using the TALOS-N server. Taken together, these data are essential to perform NMR-based experiments, including structure determination, backbone dynamics, mapping ligand sites and enabling a deeper understanding of the effect of hydrophobic surface clusters, which are important elements to stabilize some 3D proteins structure/folding.

Research paper thumbnail of Toward the mechanism of jarastatin (rJast) inhibition of the integrin αVβ3

International Journal of Biological Macromolecules, Dec 31, 2023

Research paper thumbnail of Galactosaminoglycans: Medical Applications and Drawbacks

Research paper thumbnail of The Sea as a Rich Source of Structurally Unique Glycosaminoglycans and Mimetics

Microorganisms, Aug 28, 2017

Research paper thumbnail of Chemical composition determination and evaluation of the antibacterial activity of essential oils from Ruellia asperula (Mart. Ex Ness) Lindau and Ruellia paniculata L. against oral streptococci

Natural Product Research

This study investigated the chemical composition and evaluated the antibacterial and antibiofilm ... more This study investigated the chemical composition and evaluated the antibacterial and antibiofilm activities of essential oils (EOs) extracted from Ruellia asperula (EORA) and Ruellia paniculata (EORP) against oral streptococci. The EO constituents were analyzed by gas chromatography/mass spectrometry. The antimicrobial potential of EOs was evaluated using the minimum inhibitory concentration, minimum bactericidal concentration, and time-kill determination. Furthermore, the quantification of total biomass and the number of viable cells in the biofilms were evaluated. The major constituents of EORA were cariophylla-4(12)-8-(13)-dien-5β-ol (14.1%), (β)-caryophyllene (22.7%), and caryophyllene oxide (29.4%). For EORP, the major constituents were (β)-caryophyllene (11.0%), spathulenol (13.1%), and δ-amorphene (14.9%). The tested EOs exhibited antibacterial activity against planktonic growth and biofilm formation. Thus, the EOs from R. asperula and R. paniculata prove to be promising alternatives for bacterial growth control and biofilm formation prevention of oral streptococci.

Research paper thumbnail of Leveraging Genome Sequencing Strategies for Basic and Applied Algal Research, Exemplified by Case Studies

Handbook of Algal Technologies and Phytochemicals, 2019

Research paper thumbnail of Structure-Function Relationship of the Disintegrin Family: Sequence Signature and Integrin Interaction

Frontiers in Molecular Biosciences, 2021

Disintegrins are small cysteine-rich proteins found in a variety of snake venom. These proteins s... more Disintegrins are small cysteine-rich proteins found in a variety of snake venom. These proteins selectively modulate integrin function, heterodimeric receptors involved in cell-cell and cell-matrix interaction that are widely studied as therapeutic targets. Snake venom disintegrins emerged from the snake venom metalloproteinase and are classified according to the sequence size and number of disulfide bonds. Evolutive structure and function diversification of disintegrin family involves a stepwise decrease in the polypeptide chain, loss of cysteine residues, and selectivity. Since the structure elucidation of echistatin, the description of the structural properties of disintegrins has allowed the investigation of the mechanisms involved in integrin-cell-extracellular matrix interaction. This review provides an analysis of the structures of all family groups enabling the description of an expanded classification of the disintegrin family in seven groups. Each group presents a particul...

Research paper thumbnail of Biocompatibility and structural characterization of glycosaminoglycans isolated from heads of silver-banded whiting (Sillago argentifasciata Martin & Montalban 1935)

International Journal of Biological Macromolecules, 2020

Research paper thumbnail of Galactosaminoglycans: Medical Applications and Drawbacks

Molecules, 2019

Galactosaminoglycans (GalAGs) are sulfated glycans composed of alternating N-acetylgalactosamine ... more Galactosaminoglycans (GalAGs) are sulfated glycans composed of alternating N-acetylgalactosamine and uronic acid units. Uronic acid epimerization, sulfation patterns and fucosylation are modifications observed on these molecules. GalAGs have been extensively studied and exploited because of their multiple biomedical functions. Chondroitin sulfates (CSs), the main representative family of GalAGs, have been used in alternative therapy of joint pain/inflammation and osteoarthritis. The relatively novel fucosylated chondroitin sulfate (FCS), commonly found in sea cucumbers, has been screened in multiple systems in addition to its widely studied anticoagulant action. Biomedical properties of GalAGs are directly dependent on the sugar composition, presence or lack of fucose branches, as well as sulfation patterns. Although research interest in GalAGs has increased considerably over the three last decades, perhaps motivated by the parallel progress of glycomics, serious questions concernin...

Research paper thumbnail of Marine Carbohydrate-Based Compounds with Medicinal Properties

The oceans harbor a great diversity of organisms, and have been recognized as an important source... more The oceans harbor a great diversity of organisms, and have been recognized as an important source of new compounds with nutritional and therapeutic potential. Among these compounds, carbohydrate-based compounds are of particular interest because they exhibit numerous biological functions associated with their chemical diversity. This gives rise to new substances for the development of bioactive products. Many are the known applications of substances with glycosidic domains obtained from marine species. This review covers the structural properties and the current findings on the antioxidant, anti-inflammatory, anticoagulant, antitumor and antimicrobial activities of medium and high molecular-weight carbohydrates or glycosylated compounds extracted from various marine organisms.

Research paper thumbnail of The Sea as a Rich Source of Structurally Unique Glycosaminoglycans and Mimetics

Research paper thumbnail of Antimicrobial Effect of the Triterpene 3β,6β,16β-Trihydroxylup-20(29)-ene on Planktonic Cells and Biofilms from Gram Positive and Gram Negative Bacteria

BioMed Research International, 2014

This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a tri... more This study evaluated the antimicrobial effect of 3β,6β,16β-trihydroxylup-20(29)-ene (CLF1), a triterpene isolated fromCombretum leprosumMart., in inhibiting the planktonic growth and biofilms of Gram positive bacteriaStreptococcus mutansandS. mitis. The antimicrobial activity was assessed by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibiofilm potential was determined by quantifying total biomass and enumerating biofilm-entrapped viable bacteria. In addition, the acute toxicity of CLF1 onArtemiasp. nauplii was also determined. The results showed that CLF1 was able in inhibiting the growth ofS. mutansandS. mitiswith MIC and MBC of 7.8 μg/mL and 15.6 μg/mL, respectively. CLF1 was highly effective on biofilms of both bacteria. Only 7.8 μg/mL CLF1 was enough to inhibit by 97% and 90% biomass production ofS. mutansandS. mitis, respectively. On the other hand, such effects were not evident on Gram negativePseudomonas aerugino...

Research paper thumbnail of 15N, 13C, and 1H resonance assignments of Jarastatin: a disintegrin of Bothrops jararaca

Biomolecular NMR Assignments

Disintegrins are a group of cysteine-rich proteins found in a wide variety of snake venoms. These... more Disintegrins are a group of cysteine-rich proteins found in a wide variety of snake venoms. These proteins selectively bind to integrins, which play a fundamental role in the regulation of many physiological and pathological processes. Here, we report the NMR chemical shift assignments for 1H, 15N, and 13C nuclei in the backbone and side chains of recombinant disintegrin Jarastatin (rJast), which was further validated by secondary structure prediction using the TALOS-N server. Taken together, these data are essential to perform NMR-based experiments, including structure determination, backbone dynamics, mapping ligand sites and enabling a deeper understanding of the effect of hydrophobic surface clusters, which are important elements to stabilize some 3D proteins structure/folding.