Vassilia Partali - Academia.edu (original) (raw)
Papers by Vassilia Partali
Médecine des Maladies Métaboliques, 2013
Médecine des maladies Métaboliques Dossier thématique 203 205 Avant-propos Médico-économie de la ... more Médecine des maladies Métaboliques Dossier thématique 203 205 Avant-propos Médico-économie de la santé A. Grimaldi 207 Le paiement à la performance est-il performant ? L. Degos 212 Le diabétique et le mythe du catoblépas : faut-il réformer le système des affections de longue durée (ALD) remboursées à 100 % ? A. Coulomb, J.-P. Durand, R. Cash 220 Évaluation économique des stratégies et des produits de santé : de la théorie à la pratique I. Durand-Zaleski 224 Aspects économiques du diabète en France : quelles évolutions ?
The Journal of Physical Chemistry A, 2015
Three zeaxanthin homologs with conjugation lengths N of 15, 19 and 23 denoted as Z15, Z19 and Z23... more Three zeaxanthin homologs with conjugation lengths N of 15, 19 and 23 denoted as Z15, Z19 and Z23 were studied by femtosecond transient absorption spectroscopy and the results were compared with those obtained for zeaxanthin (Z11). Energies of the S2 decrease from 20,450 cm-1 (Z11) to 18,280 cm(-1) (Z15), 17,095 cm(-1) (Z19) and 16,560 cm(-1) (Z23). Fitting the N-dependence of the S2 energies allowed estimation of E∞, the S2 energy of a hypothetical infinite zeaxanthin, to be ~14000 cm(-1). Exciting the 0-0 band of the S2 state produces characteristic S1-Sn spectral profiles in transient absorption spectra with maxima at 556 nm (Z11), 630 nm (Z15), 690 nm (Z19) and 740 nm (Z23). The red shift of the S1-Sn transition with increasing conjugation length is caused by decrease of the S1 state energy, resulting in S1 lifetimes of 9 ps (Z11), 0.9 ps (Z15), 0.35 ps (Z19) and 0.19 ps (Z23). Essentially the same lifetimes were obtained after excess energy excitation at 400 nm, but the S1-Sn becomes broader indicating larger conformation disorder in the S1 state after 400 nm excitation compared to excitation into the 0-0 band of the S2 state. An S* signal was observed in all samples, but only for Z15, Z19 and Z23 the S* signal decays with a lifetime different from the S1 state. The S* lifetimes are 2.9 and 1.6 ps for Z15 and Z19, respectively. In Z23 the S* signal needs two decay components yielding lifetimes of 0.24 and 2.3 ps. The S* signal is more pronounced after 400 nm excitation.
Acta Chemica Scandinavica, 1986
Tetrahedron Letters, 2012
Organic Letters, 2012
A microwave assisted Wittig reaction allowed the synthesis, in good yields, of the longest polyen... more A microwave assisted Wittig reaction allowed the synthesis, in good yields, of the longest polyene so far recorded with 27 conjugated double bonds. The synthesis of this stable, well-soluble polyene represents a noteworthy step in the direction of ultimate λ(max).
Molecules, 2012
Duchenne Muscular Dystrophy (DMD) is a common, inherited, incurable, fatal muscle wasting disease... more Duchenne Muscular Dystrophy (DMD) is a common, inherited, incurable, fatal muscle wasting disease caused by deletions that disrupt the reading frame of the DMD gene such that no functional dystrophin protein is produced. Antisense oligonucleotide (AO)-directed exon skipping restores the reading frame of the DMD gene, and truncated, yet functional dystrophin protein is expressed. The aim of this study was to assess the efficiency of two novel rigid, cationic carotenoid lipids, C30-20 and C20-20, in the delivery of a phosphorodiamidate morpholino (PMO) AO, specifically designed for the targeted skipping of exon 45 of DMD mRNA in normal human skeletal muscle primary cells (hSkMCs). The cationic carotenoid lipid/PMO-AO lipoplexes yielded significant exon 45 skipping relative to a known commercial lipid, 1,2-dimyristoyl-sn-glycero-3ethylphosphocholine (EPC).
![Research paper thumbnail of O1-[6-(Methylselanyl)hexanoyl]glycerol as an Anchor for Self-Assembly of Biological Compounds at the Gold Surface](https://attachments.academia-assets.com/98988408/thumbnails/1.jpg)
](Collection of Czechoslovak Chemical Communications, 2004
Adsorption ofO1-[6-(methylselanyl)hexanoyl]glycerol (SeOG) on the gold surface was investigated b... more Adsorption ofO1-[6-(methylselanyl)hexanoyl]glycerol (SeOG) on the gold surface was investigated by cyclic voltammetry, phase-sensitive AC voltammetry, electrochemical impedance spectroscopy and piezoelectric microgravimetry. SeOG adsorption results in a stable and compact surface layer with the coverage degree close to unity for an adsorption time of 30 to 80 min and 4.6 mM SeOG acetonitrile solution. Such a layer displays minute defects (pinholes) with the radius of ca. 1-3 μm, separated by 6-50 μm intervals (depending on the adsorption time). The adsorbed compound undergoes anodic desorption in the gold oxide region and also undergoes a cathodic process leading to the removal of the surface layer. Both these processes are similar to those demonstrated by short-chain alkanethiols and have been interpreted as a indication for the conversion of the selena to selenol function as a result of a dissociative adsorption process. Apparently, the main component of the surface layer isO1-(6-...
Molecules, 2012
The success of nucleic acid delivery requires the development of safe and efficient delivery vect... more The success of nucleic acid delivery requires the development of safe and efficient delivery vectors that overcome cellular barriers for effective transport. Herein we describe the synthesis of a series of novel, single-chain rigid cationic carotenoid lipids and a study of their preliminary in vitro siRNA delivery effectiveness and cellular toxicity. The efficiency of siRNA delivery by the single-chain lipid series was compared with that of known cationic lipid vectors, 3β-[N-(N',N'-dimethylaminoethane)carbamoyl]-cholesterol (DC-Chol) and 1,2-dimyristoyl-sn-glyceryl-3-phosphoethanolamine (EPC) as positive controls. All cationic lipids (controls and single-chain lipids) were co-formulated into liposomes with the neutral co-lipid, 1,2-dioleolyl-sn-glycerol-3-phosphoethanolamine (DOPE). Cationic lipid-siRNA complexes of varying (+/−) molar charge ratios were formulated for delivery into HR5-CL11 cells. Of the five single-chain carotenoid lipids investigated, lipids 1, 2, 3 and 5 displayed significant knockdown efficiency with HR5-CL11 cells. In addition, lipid 1 exhibited the lowest levels of cytotoxicity with cell viability greater than 80% at all (+/−) molar charge ratios studied. This novel, single-chain
Chemistry and physics of lipids, 2018
The term "fatty acids" is conceptually well defined with regard to fats, whose extent o... more The term "fatty acids" is conceptually well defined with regard to fats, whose extent of saturation or unsaturation is precisely indicated in the content description of foodstuff. In contrast, the term "fatty acid" gives no hint of being associated with "soap" (Na and K salts of fatty acids). Fatty acids in edible fats or in cleaning soaps have one thing in common: they are colorless. The prevalence of colorless fats and unadulterated white soaps has ensured that fatty acids are not associated with color. However, colored conjugated polyunsaturated fatty acids do exist, occurring abundantly in nature or manufactured at large scale. We endeavor to extricate conjugated polyenoic acids from oblivion by Based on the presented results (alkalicarotenoates have similar surface properties to alkalicarboxylates, carotenoic acids react like carboxylic acids to lipids), we argue for inclusion of conjugated carotenoic acids in fatty acid inventories and organic che...
Chemischer Informationsdienst, 1986
Nutrition, Analysis and Technology, 2016
ABSTRACT Diss. rer. nat. Freiburg/Schweiz.
Chemistry and Physics of Lipids j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m /... more Chemistry and Physics of Lipids j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / c h e m p h y s l i p Hydrophilic carotenoids: facile syntheses of carotenoid oxime hydrochlorides as long-chain, highly unsaturated cationic (bola)amphiphiles a b s t r a c t Stable cationic carotenoid aggregates — predominantly of the J-type — develop when the hydrochlorides of carotenoid aldoximes and ketoximes are exposed to water. The oxime hydrochlorides are obtained by simple syntheses from commercially available food color carotenoids. Bluish-purple, unstable transient compounds were observed during hydrochlorination performed at liquid nitrogen temperature.
Acta Chemica Scandinavica, 1988
Helvetica Chimica Acta, 1985
ABSTRACT It is shown that 1,1-dimethyl-1,2,3,4-tetrahydroquinolinium ions undergo, under direct i... more ABSTRACT It is shown that 1,1-dimethyl-1,2,3,4-tetrahydroquinolinium ions undergo, under direct irradiation through quartz in CH3OH and independent of the nature of the counterion (I−, BF), a reductive cleavage of the N(1)–C(8a) bond (photo-Emde degradation). The corresponding N,N-dimethyl-3-phenylpropylamines are formed in high yields and without contamination by Hofmann degradation products of the tetrahydroquinolinium salts. Me groups at C(2) as well as substituents at C(6) (CH3, Cl, CH3O) favour the photo-Emde degradation. The aromatic Cl-substituent is reductively split off in the course of the photoreaction.
… and Physiology Part B: …, 1987
Médecine des Maladies Métaboliques, 2013
Médecine des maladies Métaboliques Dossier thématique 203 205 Avant-propos Médico-économie de la ... more Médecine des maladies Métaboliques Dossier thématique 203 205 Avant-propos Médico-économie de la santé A. Grimaldi 207 Le paiement à la performance est-il performant ? L. Degos 212 Le diabétique et le mythe du catoblépas : faut-il réformer le système des affections de longue durée (ALD) remboursées à 100 % ? A. Coulomb, J.-P. Durand, R. Cash 220 Évaluation économique des stratégies et des produits de santé : de la théorie à la pratique I. Durand-Zaleski 224 Aspects économiques du diabète en France : quelles évolutions ?
The Journal of Physical Chemistry A, 2015
Three zeaxanthin homologs with conjugation lengths N of 15, 19 and 23 denoted as Z15, Z19 and Z23... more Three zeaxanthin homologs with conjugation lengths N of 15, 19 and 23 denoted as Z15, Z19 and Z23 were studied by femtosecond transient absorption spectroscopy and the results were compared with those obtained for zeaxanthin (Z11). Energies of the S2 decrease from 20,450 cm-1 (Z11) to 18,280 cm(-1) (Z15), 17,095 cm(-1) (Z19) and 16,560 cm(-1) (Z23). Fitting the N-dependence of the S2 energies allowed estimation of E∞, the S2 energy of a hypothetical infinite zeaxanthin, to be ~14000 cm(-1). Exciting the 0-0 band of the S2 state produces characteristic S1-Sn spectral profiles in transient absorption spectra with maxima at 556 nm (Z11), 630 nm (Z15), 690 nm (Z19) and 740 nm (Z23). The red shift of the S1-Sn transition with increasing conjugation length is caused by decrease of the S1 state energy, resulting in S1 lifetimes of 9 ps (Z11), 0.9 ps (Z15), 0.35 ps (Z19) and 0.19 ps (Z23). Essentially the same lifetimes were obtained after excess energy excitation at 400 nm, but the S1-Sn becomes broader indicating larger conformation disorder in the S1 state after 400 nm excitation compared to excitation into the 0-0 band of the S2 state. An S* signal was observed in all samples, but only for Z15, Z19 and Z23 the S* signal decays with a lifetime different from the S1 state. The S* lifetimes are 2.9 and 1.6 ps for Z15 and Z19, respectively. In Z23 the S* signal needs two decay components yielding lifetimes of 0.24 and 2.3 ps. The S* signal is more pronounced after 400 nm excitation.
Acta Chemica Scandinavica, 1986
Tetrahedron Letters, 2012
Organic Letters, 2012
A microwave assisted Wittig reaction allowed the synthesis, in good yields, of the longest polyen... more A microwave assisted Wittig reaction allowed the synthesis, in good yields, of the longest polyene so far recorded with 27 conjugated double bonds. The synthesis of this stable, well-soluble polyene represents a noteworthy step in the direction of ultimate λ(max).
Molecules, 2012
Duchenne Muscular Dystrophy (DMD) is a common, inherited, incurable, fatal muscle wasting disease... more Duchenne Muscular Dystrophy (DMD) is a common, inherited, incurable, fatal muscle wasting disease caused by deletions that disrupt the reading frame of the DMD gene such that no functional dystrophin protein is produced. Antisense oligonucleotide (AO)-directed exon skipping restores the reading frame of the DMD gene, and truncated, yet functional dystrophin protein is expressed. The aim of this study was to assess the efficiency of two novel rigid, cationic carotenoid lipids, C30-20 and C20-20, in the delivery of a phosphorodiamidate morpholino (PMO) AO, specifically designed for the targeted skipping of exon 45 of DMD mRNA in normal human skeletal muscle primary cells (hSkMCs). The cationic carotenoid lipid/PMO-AO lipoplexes yielded significant exon 45 skipping relative to a known commercial lipid, 1,2-dimyristoyl-sn-glycero-3ethylphosphocholine (EPC).
Collection of Czechoslovak Chemical Communications, 2004
Adsorption ofO1-[6-(methylselanyl)hexanoyl]glycerol (SeOG) on the gold surface was investigated b... more Adsorption ofO1-[6-(methylselanyl)hexanoyl]glycerol (SeOG) on the gold surface was investigated by cyclic voltammetry, phase-sensitive AC voltammetry, electrochemical impedance spectroscopy and piezoelectric microgravimetry. SeOG adsorption results in a stable and compact surface layer with the coverage degree close to unity for an adsorption time of 30 to 80 min and 4.6 mM SeOG acetonitrile solution. Such a layer displays minute defects (pinholes) with the radius of ca. 1-3 μm, separated by 6-50 μm intervals (depending on the adsorption time). The adsorbed compound undergoes anodic desorption in the gold oxide region and also undergoes a cathodic process leading to the removal of the surface layer. Both these processes are similar to those demonstrated by short-chain alkanethiols and have been interpreted as a indication for the conversion of the selena to selenol function as a result of a dissociative adsorption process. Apparently, the main component of the surface layer isO1-(6-...
Molecules, 2012
The success of nucleic acid delivery requires the development of safe and efficient delivery vect... more The success of nucleic acid delivery requires the development of safe and efficient delivery vectors that overcome cellular barriers for effective transport. Herein we describe the synthesis of a series of novel, single-chain rigid cationic carotenoid lipids and a study of their preliminary in vitro siRNA delivery effectiveness and cellular toxicity. The efficiency of siRNA delivery by the single-chain lipid series was compared with that of known cationic lipid vectors, 3β-[N-(N',N'-dimethylaminoethane)carbamoyl]-cholesterol (DC-Chol) and 1,2-dimyristoyl-sn-glyceryl-3-phosphoethanolamine (EPC) as positive controls. All cationic lipids (controls and single-chain lipids) were co-formulated into liposomes with the neutral co-lipid, 1,2-dioleolyl-sn-glycerol-3-phosphoethanolamine (DOPE). Cationic lipid-siRNA complexes of varying (+/−) molar charge ratios were formulated for delivery into HR5-CL11 cells. Of the five single-chain carotenoid lipids investigated, lipids 1, 2, 3 and 5 displayed significant knockdown efficiency with HR5-CL11 cells. In addition, lipid 1 exhibited the lowest levels of cytotoxicity with cell viability greater than 80% at all (+/−) molar charge ratios studied. This novel, single-chain
Chemistry and physics of lipids, 2018
The term "fatty acids" is conceptually well defined with regard to fats, whose extent o... more The term "fatty acids" is conceptually well defined with regard to fats, whose extent of saturation or unsaturation is precisely indicated in the content description of foodstuff. In contrast, the term "fatty acid" gives no hint of being associated with "soap" (Na and K salts of fatty acids). Fatty acids in edible fats or in cleaning soaps have one thing in common: they are colorless. The prevalence of colorless fats and unadulterated white soaps has ensured that fatty acids are not associated with color. However, colored conjugated polyunsaturated fatty acids do exist, occurring abundantly in nature or manufactured at large scale. We endeavor to extricate conjugated polyenoic acids from oblivion by Based on the presented results (alkalicarotenoates have similar surface properties to alkalicarboxylates, carotenoic acids react like carboxylic acids to lipids), we argue for inclusion of conjugated carotenoic acids in fatty acid inventories and organic che...
Chemischer Informationsdienst, 1986
Nutrition, Analysis and Technology, 2016
ABSTRACT Diss. rer. nat. Freiburg/Schweiz.
Chemistry and Physics of Lipids j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m /... more Chemistry and Physics of Lipids j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / c h e m p h y s l i p Hydrophilic carotenoids: facile syntheses of carotenoid oxime hydrochlorides as long-chain, highly unsaturated cationic (bola)amphiphiles a b s t r a c t Stable cationic carotenoid aggregates — predominantly of the J-type — develop when the hydrochlorides of carotenoid aldoximes and ketoximes are exposed to water. The oxime hydrochlorides are obtained by simple syntheses from commercially available food color carotenoids. Bluish-purple, unstable transient compounds were observed during hydrochlorination performed at liquid nitrogen temperature.
Acta Chemica Scandinavica, 1988
Helvetica Chimica Acta, 1985
ABSTRACT It is shown that 1,1-dimethyl-1,2,3,4-tetrahydroquinolinium ions undergo, under direct i... more ABSTRACT It is shown that 1,1-dimethyl-1,2,3,4-tetrahydroquinolinium ions undergo, under direct irradiation through quartz in CH3OH and independent of the nature of the counterion (I−, BF), a reductive cleavage of the N(1)–C(8a) bond (photo-Emde degradation). The corresponding N,N-dimethyl-3-phenylpropylamines are formed in high yields and without contamination by Hofmann degradation products of the tetrahydroquinolinium salts. Me groups at C(2) as well as substituents at C(6) (CH3, Cl, CH3O) favour the photo-Emde degradation. The aromatic Cl-substituent is reductively split off in the course of the photoreaction.
… and Physiology Part B: …, 1987