Vassilios Roussis - Academia.edu (original) (raw)
Papers by Vassilios Roussis
Steroids, Nov 1, 2016
Seven new (1-3, 5 and 8-10) and three previously reported (4, 6 and 7) 4α-methylated steroids wer... more Seven new (1-3, 5 and 8-10) and three previously reported (4, 6 and 7) 4α-methylated steroids were isolated from the organic extract of the gorgonian Litophyton mollis. The structures and the relative configurations of the isolated natural products were determined on the basis of extensive analyses of their NMR and MS data. Metabolites 1 and 5-8 exhibited cytotoxic activity against K562 human chronic myelogenous leukemia cells with IC50 values below 10μM, while at the same time displaying low toxicity against healthy PBMCs.
Langmuir, Sep 23, 2020
Construction of extracellular matrix-mimetic nanofilms has considerable potential in biomedical a... more Construction of extracellular matrix-mimetic nanofilms has considerable potential in biomedical and nanomedicinal fields. In this work, we fabricated neurocompatible layer-by-layer (LbL) films based on ulvan (ULV), a highly sulfated polysaccharide having compositional similarity to glycosaminoglycans that play important functional roles in the brain. ULV was durably assembled as a film with chitosan, another marine-derived polysaccharide, and the film enabled the stable adhesion of primary hippocampal neurons with high viability, comparable to the conventional poly-D-lysine surface. Notably, the ULV-based LbL films accelerated neurite outgrowth and selectively suppressed the adhesion of astrocytes, highlighting its potential as an advanced platform for neural implants and devices.
African Journal of Traditional, Complementary and Alternative Medicines, Jul 27, 2010
Acacia mellifera has been used widely in traditional African medicines against various diseases. ... more Acacia mellifera has been used widely in traditional African medicines against various diseases. Among the Kipsigis community of Kenya, water extracts from the plant is used for the treatment of skin diseases, coughs and gastrointestinal ailments. The aim of the study was to provide scientific rationale for the use of the plant in traditional medicine through bioassay-guided fractionation of A. mellifera stem bark. Bioactivity testing was done against selected microbes using disc diffusion technique as outlined in Clinical Laboratory Standard Institute (CLSI). Structure elucidation of the isolated compounds was based primarily on 1D and 2D NMR analyses, including HMQC, HMBC, and NOESY correlations. Fractionation yielded three triterpenoids; (20S)-oxolupane-30al, (20R)-oxolupane-30-al, and betulinic acid. The three compounds were active against Staphylococcus aureus ATCC 25923 and only (20S)-oxolupane-30-al against clinical isolate of Microsporum gypseum. The three compounds had no activity against Escherichia coli ATCC 25922, Enterococcus feacalis, Candida albicans ATCC 90028, Cryptococcus neoformans, Trichophyton mentagrophyte, Candida krusei, Microsporum gypseum, and Sacharomyces cerevisiae. These results explain and support the use of A. mellifera stem barks for the treatment of infectious diseases in traditional Kenya medicine.It also shows that the antimicrobial activity is concentrated in the triterpenoid fractions.
ChemInform, Mar 28, 2006
Terpenes U 0200 Atomarianones A and B: Two Cytotoxic Meroditerpenes from the Brown Alga Taonia at... more Terpenes U 0200 Atomarianones A and B: Two Cytotoxic Meroditerpenes from the Brown Alga Taonia atomaria.-Significant activity is observed for both constituents in tests against lung cancer cell lines.-(ABATIS, D.; VAGIAS, C.; GALANAKIS, D.;
Zeitschrift für Naturforschung C, Oct 1, 2003
Essential oils of Nepeta parnassica, collected at different developmental stages, were analyzed b... more Essential oils of Nepeta parnassica, collected at different developmental stages, were analyzed by means of GC/MS. From the fifty-five identified constituents in samples A and B, representing 94.8 % and 98.7 % of the oils respectively, 4aα,7α,7a-nepetalactone (22.0 %), 1,8-cineole (21.1 %), α-pinene (9.5 %) and 4aα,7 ,7a-nepetalactone (7.9 %) were the major components of sample A (vegetative stage), whereas in sample B (flowering stage) the main contributors were 1,8-cineole (34.6 %), 4aα,7α,7aα-nepetalactone (17.3 %), α-pinene (11.4 %) and 4aα,7α,7a-nepetalactone (8.9 %). The oils were tested on human health important insects such as the Pogonomyrmex sp. ants and the Culex pipiens molestus mosquitoes with promising results on insect repellency/toxicity.
Marine Drugs
Periodontitis is a microbially-induced inflammation of the periodontium that is characterized by ... more Periodontitis is a microbially-induced inflammation of the periodontium that is characterized by the destruction of the periodontal ligament (PDL) and alveolar bone and constitutes the principal cause of teeth loss in adults. Periodontal tissue regeneration can be achieved through guided tissue/bone regeneration (GTR/GBR) membranes that act as a physical barrier preventing epithelial infiltration and providing adequate time and space for PDL cells and osteoblasts to proliferate into the affected area. Electrospun nanofibrous scaffolds, simulating the natural architecture of the extracellular matrix (ECM), have attracted increasing attention in periodontal tissue engineering. Carrageenans are ideal candidates for the development of novel nanofibrous GTR/GBR membranes, since previous studies have highlighted the potential of carrageenans for bone regeneration by promoting the attachment and proliferation of osteoblasts. Herein, we report the development of bi- and tri-layer nanofibrou...
Medicinski arhiv, 2019
Introduction: Diabetic foot ulcer (DFU) is a common lower-extremity complication in patients with... more Introduction: Diabetic foot ulcer (DFU) is a common lower-extremity complication in patients with diabetes mellitus. A novel DFU treatment is tested by using an ointment containing as healing agent olive oil isopod Ceratothoa oestroidesextract. Case report: A 58 years old obese man,smoker, with a history of unregulated Type 2 Diabetes Mellitus, peripheral neuropathy and Hodgkin lymphoma was referred to Athens-Greece university hospital Laikon. The patient presented clinically with a lower extremity DFU and peripheral neuropathy with dysesthesia and disturbed sensation of hot and cold. He was treated with an ointment containing C. oestroides extract for five months, without any antimicrobial treatment. Therapy was evaluated by measurement of the transepidermal water loss, skin hydration, photo documentation and planimetry. At each patient's visit, DFU presented a satisfactory healing process. Five months after treatment initiation the patient had complete healing of his DFU. Blood tests after treatment revealed a significant reduction of the levels of the inflammatory markers. Ulcer cultures did not reveal any microbial development neither before nor after treatment. Conclusion: The administration of the C. oestroides extract ointment proved to be effective in this case. Although these results should be further investigated, the reported case suggests a novel option for the management of neuropathic diabetic foot ulcers, especially in patients with severe co-morbidities.
European Journal of Pharmaceutical Sciences, 2019
Non-invasive biophysical methods were used to study the effect of antioxidant gels, which were pr... more Non-invasive biophysical methods were used to study the effect of antioxidant gels, which were prepared from Pinus halepensis bark extracts, vitamin C and water, on the skin of diabetic hairless mice irradiated with UV radiation of 1 and 2 minimal erythemal doses (MEDs). The calculated transepidermal water loss (TEWL) for diabetic mice was found to be fivefold higher on day 11 after irradiation, and in all cases, the TEWL values converged to their initial values on day 21. Both pinus and vitamin C gels inhibited the dehydration of the skin, while water gels did not show similar protection. At low dose of UV-irradiation (1 MED), vitamin C gels showed the best hydration, while by doubling the UV dose, pinus gels induced significant skin-protective effects. Upon irradiation, the mice treatment with pinus gel showed diminished inflammation in comparison with the other gels. Pinus also inhibited the hyperkeratosis of skin. As expected, 2 MEDs caused greater skin damage, such as inflammation, dryness, oxidative stress and rides (aging) compared to the damage induced by 1 MED.
Wound Repair and Regeneration, Nov 27, 2019
Diabetic foot ulceration is a common and severe complication of diabetes, causing substantial soc... more Diabetic foot ulceration is a common and severe complication of diabetes, causing substantial social, medical and economic burdens. Treatment of foot ulcers remains challenging, thus requiring increasing awareness and more efficient management. This study investigates the efficacy of ointments, containing as main active ingredient the olive oil extract of the marine isopod Ceratothoa oestroides, in the treatment of patients with diabetic foot ulcers. Fifty-two patients were allocated into four treatment groups either receiving therapy with an ointment containing extract of C. oestroides or extract of C. oestroides and eosin or extract of C. oestroides and cefaclor or no treatment. Patients were monitored for a period of 135 days by evaluation of transepidermal water loss, skin hydration, planimetry, photo-documentation and clinical condition. Treatment with the extract of C. oestroides demonstrated significant healing properties that became evident after 45 days of treatment and resulted in complete ulcer healing in 61% of the patients. A significant improvement in transepidermal water loss (p<0.001), skin hydration levels (p<0.001) and wound area (p<0.001) was observed in all patients. Similar efficacy was demonstrated for the combination of C. oestroides extract with eosin treatment (p<0.001). On the contrary, the combination of C. oestroides extract with cefaclor antibiotic agent completely inhibited the healing properties of the isopod extract and did not improve water loss, skin hydration or wound area. An important factor for C. oestroides extract healing properties is it's selective activity against Gram negative bacteria. Ointments containing C. oestroides extract alone or combined with the antimicrobial agent eosin emerges as an effective regimen for the treatment of diabetic foot ulcers.
Nature Communications, Sep 3, 2022
numerous hydrocarbon skeletons by the activity of terpene synthases 1. Subsequently, decorating e... more numerous hydrocarbon skeletons by the activity of terpene synthases 1. Subsequently, decorating enzymes (cytochrome P450s, dehydrogenases, O-acetyltransferases, O-methyltransferases, etc.) expand the complexity of the terpene scaffolds to produce a broad diversity of compounds within each specific class (Fig. 1). As the biosynthesis of isoprenoids is based on the C 5 isoprene unit, the different isoprenoid classes differ in their size by five carbon atoms. This creates a stratification in the possible chemical structures and restricts the complexity of isoprenoids to skeletons of 10, 15, 20, or higher multiples of five carbon atoms 1. Only very few molecules with a 4b TPP 4c HaKS /Cyc2 hydrolysis
Journal of Essential Oil Research, May 1, 2001
The terpenoid composition of the needle oils of syntopically grown Pinus armandi Franch, P. densi... more The terpenoid composition of the needle oils of syntopically grown Pinus armandi Franch, P. densiflora Sieb. & Zucc., P. longaeva Bailey, P. mugo Turra, P. pumila (Pall.) Regel and P. sylvestris L. were analyzed by GC and GC/MS. Seventy-six compounds were identified in the oils. P. armandi was found to be rich in β-caryophyllene (36.3%) and γ-muurolene (40.7%). P. densiflora contained α-pinene (13.2%), β-phellandrene (11.6%) and germacrene D as major compounds. The predominant compounds in P. longaeva oil were α-pinene (17.1%), β-pinene (17.7%) and β-phellandrene (32.5%), the major component of P. mugo oil was α-pinene (33.3%) while P. pumila oil was rich in δ-3-carene (14.9%), β-phellandrene (10.7%) and terpinolene (37.7%). An unusual strain of P. sylvestris possessed an oil rich in caryophyllene oxide (12.7%) and manoyl oxide (30.2%) rather than the usual monoterpene hydrocarbons
International journal of medical and pharmaceutical case reports, Jan 10, 2017
Aim: A case of a successful treatment using an ointment based on the olive oil extract of the iso... more Aim: A case of a successful treatment using an ointment based on the olive oil extract of the isopod Ceratothoa oestroides and eosin sodium solution is described. Presentation of Case: Obese woman with unregulated Type 2 Diabetes Mellitus, renal impairment Case Study
Marine Drugs, Apr 18, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Marine Drugs
The chemical investigation of the organic extract of the red alga Laurencia majuscula collected f... more The chemical investigation of the organic extract of the red alga Laurencia majuscula collected from Hurghada reef in the Red Sea resulted in the isolation of five C15 acetogenins, including four tricyclic ones of the maneonene type (1–4) and a 5-membered one (5), 15 sesquiterpenes, including seven lauranes (6–12), one cuparane (13), one seco-laurane (14), one snyderane (15), two chamigranes (16, 17), two rearranged chamigranes (18, 19) and one aristolane (20), as well as a tricyclic diterpene (21) and a chlorinated fatty acid derivative (22). Among them, compounds 1–3, 5, 7, 8, 10, 11 and 14 are new natural products. The structures and the relative configurations of the isolated natural products have been established based on extensive analysis of their NMR and MS data, while the absolute configuration of maneonenes F (1) and G (2) was determined on the basis of single-crystal X-ray diffraction analysis. The anti-inflammatory activity of compounds 1, 2, 4–8, 10, 12–16, 18 and 20–22...
Marine Drugs
In search of alternative and sustainable sources of collagenous materials for biomedical applicat... more In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secon...
Journal of Drug Delivery Science and Technology
Frontiers in Marine Science, 2021
Biomass is defined as organic matter from living organisms represented in all kingdoms. It is rec... more Biomass is defined as organic matter from living organisms represented in all kingdoms. It is recognized to be an excellent source of proteins, polysaccharides and lipids and, as such, embodies a tailored feedstock for new products and processes to apply in green industries. The industrial processes focused on the valorization of terrestrial biomass are well established, but marine sources still represent an untapped resource. Oceans and seas occupy over 70% of the Earth’s surface and are used intensively in worldwide economies through the fishery industry, as logistical routes, for mining ores and exploitation of fossil fuels, among others. All these activities produce waste. The other source of unused biomass derives from the beach wrack or washed-ashore organic material, especially in highly eutrophicated marine ecosystems. The development of high-added-value products from these side streams has been given priority in recent years due to the detection of a broad range of biopolym...
Steroids, Nov 1, 2016
Seven new (1-3, 5 and 8-10) and three previously reported (4, 6 and 7) 4α-methylated steroids wer... more Seven new (1-3, 5 and 8-10) and three previously reported (4, 6 and 7) 4α-methylated steroids were isolated from the organic extract of the gorgonian Litophyton mollis. The structures and the relative configurations of the isolated natural products were determined on the basis of extensive analyses of their NMR and MS data. Metabolites 1 and 5-8 exhibited cytotoxic activity against K562 human chronic myelogenous leukemia cells with IC50 values below 10μM, while at the same time displaying low toxicity against healthy PBMCs.
Langmuir, Sep 23, 2020
Construction of extracellular matrix-mimetic nanofilms has considerable potential in biomedical a... more Construction of extracellular matrix-mimetic nanofilms has considerable potential in biomedical and nanomedicinal fields. In this work, we fabricated neurocompatible layer-by-layer (LbL) films based on ulvan (ULV), a highly sulfated polysaccharide having compositional similarity to glycosaminoglycans that play important functional roles in the brain. ULV was durably assembled as a film with chitosan, another marine-derived polysaccharide, and the film enabled the stable adhesion of primary hippocampal neurons with high viability, comparable to the conventional poly-D-lysine surface. Notably, the ULV-based LbL films accelerated neurite outgrowth and selectively suppressed the adhesion of astrocytes, highlighting its potential as an advanced platform for neural implants and devices.
African Journal of Traditional, Complementary and Alternative Medicines, Jul 27, 2010
Acacia mellifera has been used widely in traditional African medicines against various diseases. ... more Acacia mellifera has been used widely in traditional African medicines against various diseases. Among the Kipsigis community of Kenya, water extracts from the plant is used for the treatment of skin diseases, coughs and gastrointestinal ailments. The aim of the study was to provide scientific rationale for the use of the plant in traditional medicine through bioassay-guided fractionation of A. mellifera stem bark. Bioactivity testing was done against selected microbes using disc diffusion technique as outlined in Clinical Laboratory Standard Institute (CLSI). Structure elucidation of the isolated compounds was based primarily on 1D and 2D NMR analyses, including HMQC, HMBC, and NOESY correlations. Fractionation yielded three triterpenoids; (20S)-oxolupane-30al, (20R)-oxolupane-30-al, and betulinic acid. The three compounds were active against Staphylococcus aureus ATCC 25923 and only (20S)-oxolupane-30-al against clinical isolate of Microsporum gypseum. The three compounds had no activity against Escherichia coli ATCC 25922, Enterococcus feacalis, Candida albicans ATCC 90028, Cryptococcus neoformans, Trichophyton mentagrophyte, Candida krusei, Microsporum gypseum, and Sacharomyces cerevisiae. These results explain and support the use of A. mellifera stem barks for the treatment of infectious diseases in traditional Kenya medicine.It also shows that the antimicrobial activity is concentrated in the triterpenoid fractions.
ChemInform, Mar 28, 2006
Terpenes U 0200 Atomarianones A and B: Two Cytotoxic Meroditerpenes from the Brown Alga Taonia at... more Terpenes U 0200 Atomarianones A and B: Two Cytotoxic Meroditerpenes from the Brown Alga Taonia atomaria.-Significant activity is observed for both constituents in tests against lung cancer cell lines.-(ABATIS, D.; VAGIAS, C.; GALANAKIS, D.;
Zeitschrift für Naturforschung C, Oct 1, 2003
Essential oils of Nepeta parnassica, collected at different developmental stages, were analyzed b... more Essential oils of Nepeta parnassica, collected at different developmental stages, were analyzed by means of GC/MS. From the fifty-five identified constituents in samples A and B, representing 94.8 % and 98.7 % of the oils respectively, 4aα,7α,7a-nepetalactone (22.0 %), 1,8-cineole (21.1 %), α-pinene (9.5 %) and 4aα,7 ,7a-nepetalactone (7.9 %) were the major components of sample A (vegetative stage), whereas in sample B (flowering stage) the main contributors were 1,8-cineole (34.6 %), 4aα,7α,7aα-nepetalactone (17.3 %), α-pinene (11.4 %) and 4aα,7α,7a-nepetalactone (8.9 %). The oils were tested on human health important insects such as the Pogonomyrmex sp. ants and the Culex pipiens molestus mosquitoes with promising results on insect repellency/toxicity.
Marine Drugs
Periodontitis is a microbially-induced inflammation of the periodontium that is characterized by ... more Periodontitis is a microbially-induced inflammation of the periodontium that is characterized by the destruction of the periodontal ligament (PDL) and alveolar bone and constitutes the principal cause of teeth loss in adults. Periodontal tissue regeneration can be achieved through guided tissue/bone regeneration (GTR/GBR) membranes that act as a physical barrier preventing epithelial infiltration and providing adequate time and space for PDL cells and osteoblasts to proliferate into the affected area. Electrospun nanofibrous scaffolds, simulating the natural architecture of the extracellular matrix (ECM), have attracted increasing attention in periodontal tissue engineering. Carrageenans are ideal candidates for the development of novel nanofibrous GTR/GBR membranes, since previous studies have highlighted the potential of carrageenans for bone regeneration by promoting the attachment and proliferation of osteoblasts. Herein, we report the development of bi- and tri-layer nanofibrou...
Medicinski arhiv, 2019
Introduction: Diabetic foot ulcer (DFU) is a common lower-extremity complication in patients with... more Introduction: Diabetic foot ulcer (DFU) is a common lower-extremity complication in patients with diabetes mellitus. A novel DFU treatment is tested by using an ointment containing as healing agent olive oil isopod Ceratothoa oestroidesextract. Case report: A 58 years old obese man,smoker, with a history of unregulated Type 2 Diabetes Mellitus, peripheral neuropathy and Hodgkin lymphoma was referred to Athens-Greece university hospital Laikon. The patient presented clinically with a lower extremity DFU and peripheral neuropathy with dysesthesia and disturbed sensation of hot and cold. He was treated with an ointment containing C. oestroides extract for five months, without any antimicrobial treatment. Therapy was evaluated by measurement of the transepidermal water loss, skin hydration, photo documentation and planimetry. At each patient's visit, DFU presented a satisfactory healing process. Five months after treatment initiation the patient had complete healing of his DFU. Blood tests after treatment revealed a significant reduction of the levels of the inflammatory markers. Ulcer cultures did not reveal any microbial development neither before nor after treatment. Conclusion: The administration of the C. oestroides extract ointment proved to be effective in this case. Although these results should be further investigated, the reported case suggests a novel option for the management of neuropathic diabetic foot ulcers, especially in patients with severe co-morbidities.
European Journal of Pharmaceutical Sciences, 2019
Non-invasive biophysical methods were used to study the effect of antioxidant gels, which were pr... more Non-invasive biophysical methods were used to study the effect of antioxidant gels, which were prepared from Pinus halepensis bark extracts, vitamin C and water, on the skin of diabetic hairless mice irradiated with UV radiation of 1 and 2 minimal erythemal doses (MEDs). The calculated transepidermal water loss (TEWL) for diabetic mice was found to be fivefold higher on day 11 after irradiation, and in all cases, the TEWL values converged to their initial values on day 21. Both pinus and vitamin C gels inhibited the dehydration of the skin, while water gels did not show similar protection. At low dose of UV-irradiation (1 MED), vitamin C gels showed the best hydration, while by doubling the UV dose, pinus gels induced significant skin-protective effects. Upon irradiation, the mice treatment with pinus gel showed diminished inflammation in comparison with the other gels. Pinus also inhibited the hyperkeratosis of skin. As expected, 2 MEDs caused greater skin damage, such as inflammation, dryness, oxidative stress and rides (aging) compared to the damage induced by 1 MED.
Wound Repair and Regeneration, Nov 27, 2019
Diabetic foot ulceration is a common and severe complication of diabetes, causing substantial soc... more Diabetic foot ulceration is a common and severe complication of diabetes, causing substantial social, medical and economic burdens. Treatment of foot ulcers remains challenging, thus requiring increasing awareness and more efficient management. This study investigates the efficacy of ointments, containing as main active ingredient the olive oil extract of the marine isopod Ceratothoa oestroides, in the treatment of patients with diabetic foot ulcers. Fifty-two patients were allocated into four treatment groups either receiving therapy with an ointment containing extract of C. oestroides or extract of C. oestroides and eosin or extract of C. oestroides and cefaclor or no treatment. Patients were monitored for a period of 135 days by evaluation of transepidermal water loss, skin hydration, planimetry, photo-documentation and clinical condition. Treatment with the extract of C. oestroides demonstrated significant healing properties that became evident after 45 days of treatment and resulted in complete ulcer healing in 61% of the patients. A significant improvement in transepidermal water loss (p<0.001), skin hydration levels (p<0.001) and wound area (p<0.001) was observed in all patients. Similar efficacy was demonstrated for the combination of C. oestroides extract with eosin treatment (p<0.001). On the contrary, the combination of C. oestroides extract with cefaclor antibiotic agent completely inhibited the healing properties of the isopod extract and did not improve water loss, skin hydration or wound area. An important factor for C. oestroides extract healing properties is it's selective activity against Gram negative bacteria. Ointments containing C. oestroides extract alone or combined with the antimicrobial agent eosin emerges as an effective regimen for the treatment of diabetic foot ulcers.
Nature Communications, Sep 3, 2022
numerous hydrocarbon skeletons by the activity of terpene synthases 1. Subsequently, decorating e... more numerous hydrocarbon skeletons by the activity of terpene synthases 1. Subsequently, decorating enzymes (cytochrome P450s, dehydrogenases, O-acetyltransferases, O-methyltransferases, etc.) expand the complexity of the terpene scaffolds to produce a broad diversity of compounds within each specific class (Fig. 1). As the biosynthesis of isoprenoids is based on the C 5 isoprene unit, the different isoprenoid classes differ in their size by five carbon atoms. This creates a stratification in the possible chemical structures and restricts the complexity of isoprenoids to skeletons of 10, 15, 20, or higher multiples of five carbon atoms 1. Only very few molecules with a 4b TPP 4c HaKS /Cyc2 hydrolysis
Journal of Essential Oil Research, May 1, 2001
The terpenoid composition of the needle oils of syntopically grown Pinus armandi Franch, P. densi... more The terpenoid composition of the needle oils of syntopically grown Pinus armandi Franch, P. densiflora Sieb. & Zucc., P. longaeva Bailey, P. mugo Turra, P. pumila (Pall.) Regel and P. sylvestris L. were analyzed by GC and GC/MS. Seventy-six compounds were identified in the oils. P. armandi was found to be rich in β-caryophyllene (36.3%) and γ-muurolene (40.7%). P. densiflora contained α-pinene (13.2%), β-phellandrene (11.6%) and germacrene D as major compounds. The predominant compounds in P. longaeva oil were α-pinene (17.1%), β-pinene (17.7%) and β-phellandrene (32.5%), the major component of P. mugo oil was α-pinene (33.3%) while P. pumila oil was rich in δ-3-carene (14.9%), β-phellandrene (10.7%) and terpinolene (37.7%). An unusual strain of P. sylvestris possessed an oil rich in caryophyllene oxide (12.7%) and manoyl oxide (30.2%) rather than the usual monoterpene hydrocarbons
International journal of medical and pharmaceutical case reports, Jan 10, 2017
Aim: A case of a successful treatment using an ointment based on the olive oil extract of the iso... more Aim: A case of a successful treatment using an ointment based on the olive oil extract of the isopod Ceratothoa oestroides and eosin sodium solution is described. Presentation of Case: Obese woman with unregulated Type 2 Diabetes Mellitus, renal impairment Case Study
Marine Drugs, Apr 18, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Marine Drugs
The chemical investigation of the organic extract of the red alga Laurencia majuscula collected f... more The chemical investigation of the organic extract of the red alga Laurencia majuscula collected from Hurghada reef in the Red Sea resulted in the isolation of five C15 acetogenins, including four tricyclic ones of the maneonene type (1–4) and a 5-membered one (5), 15 sesquiterpenes, including seven lauranes (6–12), one cuparane (13), one seco-laurane (14), one snyderane (15), two chamigranes (16, 17), two rearranged chamigranes (18, 19) and one aristolane (20), as well as a tricyclic diterpene (21) and a chlorinated fatty acid derivative (22). Among them, compounds 1–3, 5, 7, 8, 10, 11 and 14 are new natural products. The structures and the relative configurations of the isolated natural products have been established based on extensive analysis of their NMR and MS data, while the absolute configuration of maneonenes F (1) and G (2) was determined on the basis of single-crystal X-ray diffraction analysis. The anti-inflammatory activity of compounds 1, 2, 4–8, 10, 12–16, 18 and 20–22...
Marine Drugs
In search of alternative and sustainable sources of collagenous materials for biomedical applicat... more In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secon...
Journal of Drug Delivery Science and Technology
Frontiers in Marine Science, 2021
Biomass is defined as organic matter from living organisms represented in all kingdoms. It is rec... more Biomass is defined as organic matter from living organisms represented in all kingdoms. It is recognized to be an excellent source of proteins, polysaccharides and lipids and, as such, embodies a tailored feedstock for new products and processes to apply in green industries. The industrial processes focused on the valorization of terrestrial biomass are well established, but marine sources still represent an untapped resource. Oceans and seas occupy over 70% of the Earth’s surface and are used intensively in worldwide economies through the fishery industry, as logistical routes, for mining ores and exploitation of fossil fuels, among others. All these activities produce waste. The other source of unused biomass derives from the beach wrack or washed-ashore organic material, especially in highly eutrophicated marine ecosystems. The development of high-added-value products from these side streams has been given priority in recent years due to the detection of a broad range of biopolym...