Verônica Azzolin - Academia.edu (original) (raw)

Papers by Verônica Azzolin

Teoria e Prática Trauma e Emergência - Edição 4ª

Chemosphere, 2021

Superoxide-hydrogen peroxide (S-HP), triggered by Val16Ala-SOD2 human polymorphism, may influence... more Superoxide-hydrogen peroxide (S-HP), triggered by Val16Ala-SOD2 human polymorphism, may influence the risk of depression. Therefore, it is plausible that higher basal S-anion levels and chronic inflammatory states associated with the VV-SOD2 genotype can negatively modulate the stress response associated with resilience in various species, from primitive species to humans. To test this hypothesis, Eisenia fetida earthworms were exposed for 24 h to 30 nM rotenone, which causes mitochondrial dysfunction by generating high S-anion levels (known as the "VV-like phenotype"), and 10 μM porphyrin, a SOD2-like compound, which generates elevated HP levels (known as the "AA-like phenotype"). The results suggested that both S-anion and HP acted as signaling molecules, differentially altering the immune function and acute hydric stressful response. Although the AA-like phenotype improved the immune and stress response efficiencies, the VV-like phenotype showed a downregulated expression of the toll-like receptor (EaTLR, JX898685) and antimicrobial peptide (AMP) (AF060552) genes, which triggered the impairment of encapsulation and earthworms extracellular trap (EET) processes used by earthworms to trap and destroy microorganisms. When exposed to adverse environments and dangerous hydric stress, VV-like earthworms exhibited an impulsive behavior and failed to quickly identify and migrate to a protected environment, unlike control earthworms and AA-like earthworms. All results corroborated that the S-anion imbalance could concomitantly induce alterations in immune function and stress behavior related to earthworm survival. From a human perspective, this information may corroborate the potential specific role of superoxide anion in the modulation of the stress response, resilience, and risk of depression.

The Journal Of Prevention of Alzheimer's Disease, 2017

Background: Vincristine (VCR) is not a specific chemotherapeutic drug, responsible for cause seve... more Background: Vincristine (VCR) is not a specific chemotherapeutic drug, responsible for cause several side effects. In this sense, many natural products have been studied to reduce this problem. Objetives: To examine the guarana neuroprotective effect in mice brain and cerebellum cells against vincristine (VCR) exposition. Design: An in vitro study was performed using mice brain and cerebellum mice in monolayer culture. First, cells were exposed to VCR (0.009 µM for 24 hours and 0.0007 µM for 72 hours) to measure the cytotoxicity effect. Also, the cellular effect of hydroalcoholic extract of guarana (10; 30; 100 and 300 μg/mL) was evaluated in the same cells in 24 and 72 hours. After that, cells were exposed to VCR and guarana extract to evaluate the neuroprotective effect of guarana. Measurements: Cell viability was analyzed by MTT, Free dsDNA and LHD Assays. Moreover, metabolism oxidative profile was evaluated by reactive oxygen species (ROS), lipoperoxidation (LPO) and catalase (C...

Introdução: O envelhecimento cutâneo, é um fenômeno fisiológico gradativo que envolve degradação ... more Introdução: O envelhecimento cutâneo, é um fenômeno fisiológico gradativo que envolve degradação do colágeno, estresse oxidativo e inflamação, esses aspectos somados ao fato de muitos idosos terem dificuldade de movimentação ou serem portadores de outras doenças como o diabetes, favorecem o aparecimento de lesões de pele de difícil cicatrização, as quais prejudicam qualidade de vida e são um problema de saúde pública. O Camu-camu (Myrciaria dúbia) é um fruto Amazônico, com alto teor de vitamina C e outras molécula bioativas que podem ter efeito pró-cicatricial. Objetivo: Avaliar os efeitos do Camu-camu na cicatrização em modelo in vitro de lesão de pele. Metodologia: Linhagem celular de fibroblastos (HFF-1) obtida comercialmente e cultivada em condições padrão, submetida a uma arranhadura (modelo de lesão in vitro) e após suplementada com extrato de Camu-camu, concentrações de 250μG/mL e 500 μG/mL, após 24 e 72 horas avaliou-se a produção de espécies reativas de oxigênio (EROs) via ...

Medical Toxicology, 2021

In this chapter the following topics will be addressed: (1) actions of the cholinergic system in ... more In this chapter the following topics will be addressed: (1) actions of the cholinergic system in the nervous system, commenting on acetylcholine metabolism and acetylcholinesterase metabolism; (2) acetylcholinesterase inhibitors as subtitle in this topic: pharmacological characterization of pyridostigmine bromide, mechanism of action, and therapeutic effect of the drug; (3) use of pyridostigmine bromide in Persian Gulf War; and (4) potential effect of pyridostigmine bromide in oxidative stress, addressing as subtitle the influence of pyridostigmine bromide on the superoxide-hydrogen peroxide imbalance model. Studies indicate that the interaction between pyridostigmine bromide and stressors could trigger genotoxicity, the mechanism associated with the induction of oxidative stress that leads to this side effect of this drug; however, this discussion needs to be better elucidated and may be more discussed as there is interaction between the pyridostigmine bromide and an endogenous oxi...

Biodiversidade e Biotecnologia no Brasil 2, 2020

O cafe e os chas estao entre as bebidas mais consumidas pela populacao mundial. Em comum, todas e... more O cafe e os chas estao entre as bebidas mais consumidas pela populacao mundial. Em comum, todas estas preparacoes possuem moleculas bioativas, principalmente a cafeina, sendo, portanto consideradas bebidas cafeinadas. Alem deste componente, estas bebidas apresentam outras moleculas com caracteristicas antioxidantes, antiinflamatorias, anticarcinogenicas, protegendo o organismo contra a oxidacao do LDL, atuando no combate a alergias, inflamacoes, ulceras, tumores, hepatotoxinas e inibicao da agregacao plaquetaria, reduzindo o risco de cardiopatias. Assim, o objetivo deste trabalho foi a analise in vitro da inibicao da agregacao plaquetaria em celulas mononuleares do sangue periferico tratadas com cafe, cha verde, cha preto, erva-mate e guarana. Os extratos foram produzidos em condicoes semelhantes aquelas em que os seres humanos geralmente consomem essas bebidas, na relacao de um grama de amostra para 10 mL de agua. Todas as amostras foram colocadas em contato com agua a 90±1oC por 1...

Introdução: Doença de Parkinson (DP) é resultado da associação entre fatores genéticos e ambienta... more Introdução: Doença de Parkinson (DP) é resultado da associação entre fatores genéticos e ambientais e da combinação desses fatores com o envelhecimento, ocasionando disfunções mitocôndrias e de membrana celular culminando com o estresse oxidativo e inflamação crônica. Compostos bioativos com capacidade antioxidante e anti-inflamatória podem reduzir o estresse oxidativo. A erva-mate (Ilex paraguariensis), altamente consumida principalmente sob a forma de chás e chimarrão, é rica em compostos fotoquímicos com capacidade antioxidante. Objetivo: Investigar o papel da erva-mate em modelo in vitro de Doença de Parkinson. Métodos: A linhagem celular SHSY-5Y foi cultivada em condições padronizadas. Inicialmente as células foram expostas a uma infusão de erva-mate (10 mg/mL) por 24 horas, adicionou-se a rotenona (indutor de DP in vitro), as células permaneceram em cultivo por 72 horas. Então, foram realizadas análises relacionadas ao metabolismo oxidativo e inflamatório. Resultados: Células ...

Chronic psycho-environmental stress can induce neurological dysfunction due to an increase in cor... more Chronic psycho-environmental stress can induce neurological dysfunction due to an increase in cortisol levels. It is possible that some food supplements could attenuate its negative impact, such as avocado oil (AO), which is rich in fatty acids with beneficial effects on the brain. This hypothesis was tested by an in vitro model using undifferentiated neuroblastoma cells (SH-SY5Y) exposed to hydrocortisone (HC), an active cortisol molecule with and without AO-supplementation. Cortisol can induce oxidative stress, apoptosis events, and a lowering effect on brain-derived neurotrophic factor (BDNF), a neurogenic molecule. As AO protective effects on HC-exposed cells could involve these routes, some markers of these routes were compared among neuroblastoma cultures. In the first assay, the range concentrations of HC exposure that trigger cell mortality and range AO-concentrations that could revert the HC effect. AO at all concentrations tested (2-30 µg/ml) did not present a cytotoxic ef...

Os fibroblastos compõem as principais células da pele, sendo responsáveis pelo processo de cicatr... more Os fibroblastos compõem as principais células da pele, sendo responsáveis pelo processo de cicatrização de lesões. O estresse oxidativo, causado pela produção excessiva de Espécies Reativas de Oxigênio (EROs), é responsável por agravar o processo de envelhecimento celular, prejudicando as funções de proliferação e cicatrização dos fibroblastos, contribuindo para o aparecimento de lesões teciduais. Desta forma, torna-se relevante a busca por compostos naturais que possam auxiliar no reparo destas lesões, como é o caso do barbatimão, planta do Cerrado, utilizada pela medicina tradicional como cicatrizante devido à sua potencial atividade antioxidante. Objetivo: Avaliar in vitro o efeito do barbatimão em marcadores de estresse oxidativo de fibroblastos humanos senescentes. Métodos: Células da linhagem de fibroblastos humanos (HFF-1) foram cultivadas em condições controladas até atingirem morfologia senescente via observação por microscopia confocal. As células senescentes foram exposta...

Acta Scientiarum Polonorum Technologia Alimentaria, 2021

Journal of Medicinal Food, 2020

Solanum sessiliflorum is an Amazonian fruit (cubiu) that has been domesticated since pre-Colombia... more Solanum sessiliflorum is an Amazonian fruit (cubiu) that has been domesticated since pre-Colombian era. It is also used in folk medicine to treat some clinical conditions. This investigation chemically characterized and analyzed the in vitro antioxidant and antitumoral effect of a cubiu pulp/seed hydroalcoholic extract. Cubiu extract was chemically characterized by high-performance liquid chromatography with diode array detector (HPLC-DAD), its antioxidant capacity measured by 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the following complementary in vitro protocols were performed: (1) cytoprotective effect of cubiu on human peripheral blood mononuclear cells (PBMCs) exposed to H2O2, a genotoxic and procarcinogen molecule; (2) effect of cubiu on low density lipoproteins oxidation; and (3) cytotoxic and antiproliferative effect on breast (MCF-7) and colorectal (HT-29) cancer cell lines. Biochemical and flow cytometry analyses were conducted in these protocols. Cubiu extract presented high concentrations of caffeic and gallic acids, beta-carotene, catechin, quercetin, and rutin, and its antioxidant capacity was confirmed. Cubiu attenuated H2O2 cytotoxicity on PBMCs, presented lowering effect on LDL oxidation, and induced mortality and proliferative inhibition of colorectal cancer cells. In cancer cells, cubiu extract at 10 μg/mL showed similar effects to 5-fluorouracil chemo drug reducing its viability and frequency of S-phase, indicating that cells are undergoing mitosis. In summary, despite the limitations of in vitro protocols, our results suggest that cubiu has several biological properties that affect human health.

Journal of Pharmacy and Pharmacology, 2018

Journal of Ethnopharmacology, 2019

ETHNOPHARMACOLOGICAL RELEVANCE The fruit of Astrocaryum aculeatum G.Mey. (tucumã) is highly consu... more ETHNOPHARMACOLOGICAL RELEVANCE The fruit of Astrocaryum aculeatum G.Mey. (tucumã) is highly consumed by riverside communities in the Amazonian region. These communities have recently been shown to have increased longevity and reduced prevalence of age-related morbidity. Tucumã, which is locally used in their diet and traditional medicine may contribute to these features. AIM OF THE STUDY To investigate the anti-inflammatory and antioxidant properties of A. aculeatum extract against phytohemagglutinin-induced inflammation in cell cultures. MATERIALS AND METHODS Cell viability and cytotoxicity assays, gene expression of interleukins IL-1β, IL-6, IL-10, levels of reactive oxygen species (ROS), nitric oxide (NO) and thiols were employed, as well as the activities of antioxidant enzymes in RAW 264.7 cells stimulated with phytohemagglutinin to mimic inflammation. RESULTS The extract of Astrocaryum aculeatum fruit inhibited macrophage proliferation (P < 0.05), arrested the cell cycle in G0/G1 phase (P < 0.001), increased antioxidant defenses (P < 0.01), reduced oxidative stress (P < 0.01), and modulated genes related to the inflammatory response (P < 0.001). CONCLUSION Our results demonstrate that A. aculeatum fruit has anti-inflammatory and antioxidant capacities. These beneficial effects of tucumã on cells are also likely to be seen in vivo, thereby suggesting that its extract is a suitable therapeutic adjuvant in the prevention or treatment of inflammatory diseases.

Food and Chemical Toxicology, 2019

Molecular Biology Reports, 2019

The purpose of this study was to investigate the effect of a superoxide-hydrogen peroxide (S-HP) ... more The purpose of this study was to investigate the effect of a superoxide-hydrogen peroxide (S-HP) imbalance of the superoxide dismutase manganese dependent (SOD2) gene, generated by paraquat and porphyrin exposure, on the keratinocytes cell line (HaCaT) oxidative metabolism. Paraquat acts increasing superoxide (O2·−) levels, while porphyrin increases hydrogen peroxide (H2O2) levels, acting as VV-SOD2-like and AA-SOD2-like molecules, respectively. First of all, HaCAT cells were treated with different concentrations of paraquat and porphyrin (1; 10; 30, and 70 μM) to determine the concentration of both that causes imbalance. After defining the concentration of paraquat and porphyrin (70 μM), a time curve was performed (1, 3, 6, and 24 h) to evaluate ROS production levels. Other oxidative parameters, such as nitric oxide (NO), lipoperoxidation (TBARS) and protein carbonyl, were evaluated after 24 h of incubation, as well as genotoxic analyses, apoptosis detection, and gene expression. Our findings revealed that paraquat exposure decreased cell viability, increasing lipoperoxidation, DNA damage, and apoptosis. On the other hand, porphyrin treatment increased cell viability and proliferation, ROS and NO production, triggering protein and DNA damage. In addition, porphyrin up-regulated Keap1 and Nrf2 gene expression, while paraquat decreased Nrf2 gene expression. In this sense, we suggested that the superoxide-hydrogen peroxide imbalance differentially modulates oxidative stress on keratinocytes cell line via Keap1-Nrf2 gene expression pathway.

Journal of Cosmetic Dermatology, 2019

Low‐level laser therapy (LLLT) has several clinical applications; however, its benefits are not u... more Low‐level laser therapy (LLLT) has several clinical applications; however, its benefits are not universal. Therefore, combination therapy with LLLT and extracts from the guarana (Paullinia cupana) plant may improve its effectiveness as guarana extracts exhibit anti‐aging properties.