Victor Ezebuiro - Academia.edu (original) (raw)
Papers by Victor Ezebuiro
IOP Conference Series: Materials Science and Engineering, 2021
This study investigated the physicochemical characteristics of fish pond effluents. Four (4) effl... more This study investigated the physicochemical characteristics of fish pond effluents. Four (4) effluent samples were collected at two-day intervals from a fish pond located at Umudiba Nekede, Owerri, and analyzed to ascertain their characteristics. The physiochemical parameters assessed are the temperature, biochemical oxygen demand, chemical oxygen demand, total dissolved solids, turbidity, ammonia, total hardness, alkalinity, pH, and electrical conductivity. The result revealed the following ranges for the physicochemical characteristics: temperature (23.0 to 25.9°C), pH (6.24 to 3.1), total alkalinity (43.1 to 50.4mg/l), total dissolved solid (27.9 to 95.2 mg/l), total hardness (19.7 to 21.5mg/l), turbidity (12 to 170 NTU), and electrical conductivity (137.6 to 144.3 μmhos/cm). The result of the study indicated that the effluents from the fish pond could constitute a threat to the ecology of the aquatic water bodies if not properly treated before discharge.
Journal of Petroleum & Environmental Biotechnology, 2016
Background/Aim: Certain plasmids play an important role in adaptation of natural microbial popula... more Background/Aim: Certain plasmids play an important role in adaptation of natural microbial populations to oil and other hydrocarbons. This research investigated the role of plasmid-borne genes in the biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a consortium of aerobic heterotrophic bacteria (AHB). Methods: Physicochemical and microbiological characteristics of the water sample were analyzed using standard methods. AHB with the capacity for biodegradation were isolated from hydrocarbon polluted water from Bodo Creek using Bushnell Haas Agar. The positive isolates were subjected to plasmid profiling and curing. Three treatments: cured, uncured and control were used in the study. Curing was achieved with acridine orange. The degradation experiment was carried out in a 10 L glass bioreactor and monitored for 56 days using GC-MS analysis. Results: Seven (7) out of 19 AHB isolates were selected for the degradation study. The 7 isolates, G1, G3, G19, GA5, GB3, GD1 and G12 were classified as Shewanella haliotis G1, Shewanella sp. G3, Vibrio alginolyticus G19, Pseudomonas putida GA5, Bacillus cereus GB3, B. pumilus GD1 and Shewanella sp. G12, respectively based on the phylogenetic analysis of their 16S rRNA genes. The sequences were deposited at the GenBank under the accession numbers KT886070-KT886076. The day 0 PAHs for cured, uncured and control treatments was 61.83 mg/L. After 56 days, the PAHs decreased to 2.90, 1.87 and 57.65 mg/L for cured, uncured and control treatments, respectively; representing percentage degradation of 95.31, 96.98 and 6.76%. The respective derived PAH degradation models for cured, uncured and control treatments were 32.614e-0.047t , 30.09e-0.05t and-0.0769t + 61.656. There was no significant difference (p<0.05) between PAH degradation in cured and uncured treatments. Conclusion: The study demonstrated that PAH degradation by the consortium used in the study was chromosomal and not plasmid-borne.
Asian Journal of Biotechnology and Bioresource Technology
Background: Production of amylase by Enterobacter cloacae D1 was optimized in this study using ce... more Background: Production of amylase by Enterobacter cloacae D1 was optimized in this study using central composite design (CCD) of response surface methodology (RSM). Methodology: Effects of five numeric factors (pH, temperature, inoculum concentration, peptone and yeast extract) on the production of amylase were examined. Amylase production was first screened using plate technique and amylase assay thereafter carried out using the dinitrosalicylic acid (DNSA) method. The CCD-RSM experimental set-up involved 30 runs with 5 levels of independent variables. Results: The amylase-producing bacterium Enterobacter cloacae strain D1 was identified based on the phylogenetic tree analysis of its sequence. The sequence has been submitted to GenBank under the accession number: MZ477010. The isolate had 98% similarity to the GenBank match Enterobacter cloacae strain ATCC 13182. Optimum conditions that yielded maximum amylase (34.43 U/mL) were pH 5; temperature 40 ℃; inoculum concentration 3%; pep...
Journal of Applied Life Sciences International, 2021
Background: Bacterial proteases represent a group of very important industrial enzymes. They are ... more Background: Bacterial proteases represent a group of very important industrial enzymes. They are involved in the hydrolysis of peptide bond found in protein. Industrial application of bacterial proteases has been limited by low yield and instability at biotechnological process conditions. This study was design to investigate the effect of temperature and pH on the stability of protease produced by Alcaligene faecalis strain P2. Methodology: Protease-producing bacteria were isolated from beans effluent-impacted soil and screened for protease production on Casein agar plate. Protease assay was carried out following standard method for protease determination and the stability of the protease produced was investigated over temperature range of 20 to 90 oC and pH range of 3 to 12. The protease-producing bacterium was identified using its molecular characteristics. Results: Protease assay result showed that the amount of tyrosine released by one unit of the crude enzyme was 0.176 µmol/mL ...
Biocatalysis and Agricultural Biotechnology
Journal of Advances in Microbiology
Aim: This study reports the production and optimization of cellulase from Penicillium sp. using c... more Aim: This study reports the production and optimization of cellulase from Penicillium sp. using corn-cob (CC) and pawpaw fibre (PF) as substrates. Methods: Nine fungal isolates, obtained from compost soil, were screened for cellulolytic activity. Isolate CPF-1, based on its ability to give the highest zones of clearance and cellulolytic activity, was selected. CPF-1 was identified as Penicillium sp. based on its cultural and morphological characteristics. Cellulase activity was determined by the DNS method on Congo red agar plate. Effects of temperature, pH and metal ions (Zn 2+ , Hg 2+ , Fe 2+ , Mg 2+ , Ca 2+ and Co 2+) on crude cellulase activity and stability were studied using two substrates (corn-cob and pawpaw fibre) by solid state fermentation.
Biotechnology Journal International
Aim: Viability of hydrocarbon-degrading bacterial consortium immobilized on different carriers wa... more Aim: Viability of hydrocarbon-degrading bacterial consortium immobilized on different carriers was studied. Methodology: Hydrocarbon-degrading bacteria were isolated from crude oil contaminated sites in Gio and K-Dera, Rivers State, Nigeria using enrichment method. Proximate analyses were carried out on the best carrier materials. Immobilization was by direct adsorption of the isolates onto the carrier materials and viability was determined by plate count method. The carrier materials tested included soya bran, sugarcane bagasse, corn cob, brown saw dust, white saw dust, cassava peel and red mud (bentonite). Results: The bacterial isolates demonstrated varied degradation capacity. The best carrier material was saw dust (103.6% survival) and corn cob (103.6% survival) followed by soya bran (94.4% survival rate) and cassava peel (94.4% survival rate). The saw dust had moisture content, 5.92%; ash content, 7.49%; crude protein, 2.2%; volatile matter, 74.28; and fixed carbon, 12.34%; wh...
Microbiology Research Journal International
Aims: To assess the quality of abattoir effluents discharged into water bodies in Owerri Municipa... more Aims: To assess the quality of abattoir effluents discharged into water bodies in Owerri Municipal, Nigeria using microbiological and physicochemical approaches. Study Design: The study employed microbiological and physicochemical parameters to determine effluent and water quality. Place and Duration of Study: Abattoirs in Owerri, Imo State, Nigeria, between September 2014 and February 2016. Methodology: Physicochemical and microbiological analyses were carried out on three abattoir effluents and their receiving water bodies. Counts of total heterotrophic bacteria, total coliform and faecal coliform, Vibrio, Salmonella and Shigella were carried using the plate count method. Results: The bacterial isolates in the various samples included members of the genera Bacillus, Citrobacter, Enterobacter, Escherichia, Klebsiella, Lactobacillus, Listeria, Micrococcus, Proteus, Salmonella, Serratia, Staphylococcus, Streptococcus and Vibrio. The order of increasing effluent’s total coliform and ...
Journal of Advances in Microbiology
Bioresources and Bioprocessing, 2016
Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria i... more Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria isolated from agrowaste-impacted soil through simultaneous saccharification and co-fermentation (SSCF) of steam-exploded bagasse was investigated. Methods: The cellulolytic (VCE-19) and xylanolytic (VXE-41) isolates were screened using the Congo Red Plate Method. The DNS method was used in the determination of reducing sugar content. Chemical analysis of the sugarcane bagasse was determined using standard methods. The bagasse was subjected to steam explosion to reduce lignin content and enhance cellulose availability. Results: Mean proximate composition analysis of the bagasse showed total carbohydrate and lignin content (% dry weight) of 70.3 ± 1.9 and 19.2 ± 1.2 before pretreatment and 85.4 ± 2.33 and 4.2 ± 0.44 after pretreatment, respectively. Phylogenetic analysis based on partial sequence of the 16S rRNA gene classified VCE-19 and VXE-41 as Bacillus cereus GBPS9 and Bacillus thuringiensis serovar kurstaki HD1, respectively. The sequences obtained from these isolates have been submitted to GenBank and accession numbers (KT350986.1 for VXE-41 and KT318371.1 for VCE-19) assigned to them. The result of the optimization of cultural conditions of the bacterial co-culture revealed optimum cellulase production at the following conditions: temperature, 40 °C; pH, 7; substrate concentration, 4.0 % (w/v); inoculum concentration, 4 % (v/v) and when yeast extract was used as nitrogen source. The gas chromatography-mass spectrometry (GC-MS) analysis of the fermentation broth detected the following components: acetone (3.49 g/L), ethylacetate (8.75 g/L), ethanol (19.08 g/L), N-propanol (4.96 g/L), isobutanol (3.73 g/L) and acetic acid (6.53 g/L). Conclusions: This study has demonstrated the production of significant quantity of ethanol by a co-culture of B. cereus GBPS9 and B. thuringiensis serovar kurstaki HD1 through SSCF of steam-exploded bagasse. Efficient bioethanol production from bagasse can help solve the need for alternative source of energy and the crisis that results from bioethanol production from food and feed crops.
Journal of Biotechnology & Biomaterials, 2015
Bioresources and Bioprocessing, May 26, 2016
Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria i... more Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria isolated from agrowaste-impacted soil through simultaneous saccharification and co-fermentation (SSCF) of steam-exploded bagasse was investigated. Methods: The cellulolytic (VCE-19) and xylanolytic (VXE-41) isolates were screened using the Congo Red Plate Method. The DNS method was used in the determination of reducing sugar content. Chemical analysis of the sugarcane bagasse was determined using standard methods. The bagasse was subjected to steam explosion to reduce lignin content and enhance cellulose availability. Results: Mean proximate composition analysis of the bagasse showed total carbohydrate and lignin content (% dry weight) of 70.3 ± 1.9 and 19.2 ± 1.2 before pretreatment and 85.4 ± 2.33 and 4.2 ± 0.44 after pretreatment, respectively. Phylogenetic analysis based on partial sequence of the 16S rRNA gene classified VCE-19 and VXE-41 as Bacillus cereus GBPS9 and Bacillus thuringiensis serovar kurstaki HD1, respectively. The sequences obtained from these isolates have been submitted to GenBank and accession numbers (KT350986.1 for VXE-41 and KT318371.1 for VCE-19) assigned to them. The result of the optimization of cultural conditions of the bacterial co-culture revealed optimum cellulase production at the following conditions: temperature, 40 °C; pH, 7; substrate concentration, 4.0 % (w/v); inoculum concentration, 4 % (v/v) and when yeast extract was used as nitrogen source. The gas chromatography-mass spectrometry (GC-MS) analysis of the fermentation broth detected the following components: acetone (3.49 g/L), ethylacetate (8.75 g/L), ethanol (19.08 g/L), N-propanol (4.96 g/L), isobutanol (3.73 g/L) and acetic acid (6.53 g/L). Conclusions: This study has demonstrated the production of significant quantity of ethanol by a co-culture of B. cereus GBPS9 and B. thuringiensis serovar kurstaki HD1 through SSCF of steam-exploded bagasse. Efficient bioethanol production from bagasse can help solve the need for alternative source of energy and the crisis that results from bioethanol production from food and feed crops.
Microbiology Research Journal International
Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfa... more Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfactant by a hydrocarbon-utilizing bacterium, Stenotrophomonas sp. Methodology: The hydrocarbon-utilizing bacterium was isolated with Bushnell Haas (BH) broth using enrichment method. Biosurfactant production was screened by evaluating the following characteristics: Emulsification index (E-24), oil spreading (displacement), tilted glass slide, haemolysis on blood agar, and lipase production. Effects of combination of nitrogen sources (yeast extract and NH4NO3, yeast extract and urea, yeast extract and asparagine, yeast extract and peptone, NaNO3 and peptone, NaNO3 and asparagine, and yeast extract and NaNO3) and carbon sources (glucose, fructose, galactose, cassava peel, soya bran, olive oil, sucrose, crude oil, diesel and glycerol) on biosurfactant production were determined with emulsion stability and surface tension as responses. The bacterium was identified based on phenotypic, microsc...
Microbiology Research Journal International
Aim: This study reports the production of cellulase by Bacillus licheniformis VVA21 isolated from... more Aim: This study reports the production of cellulase by Bacillus licheniformis VVA21 isolated from hydrocarbon contaminated Kegbara-Dere mangrove in Ogoniland, Nigeria. Methodology: Baseline physicochemical characteristics of the hydrocarbon contaminated soil were established. Twenty-two bacterial isolates were screened for cellulolytic activity on carboxymethyl cellulose (CMC) agar using the spread plate technique. The isolate with the highest zone of clearance was selected and assayed further. Crude cellulase was extracted and partial purification achieved by ammonium sulphate precipitation, followed by dialysis, and final purification Original Research Article
IOP Conference Series: Materials Science and Engineering, 2021
This study investigated the physicochemical characteristics of fish pond effluents. Four (4) effl... more This study investigated the physicochemical characteristics of fish pond effluents. Four (4) effluent samples were collected at two-day intervals from a fish pond located at Umudiba Nekede, Owerri, and analyzed to ascertain their characteristics. The physiochemical parameters assessed are the temperature, biochemical oxygen demand, chemical oxygen demand, total dissolved solids, turbidity, ammonia, total hardness, alkalinity, pH, and electrical conductivity. The result revealed the following ranges for the physicochemical characteristics: temperature (23.0 to 25.9°C), pH (6.24 to 3.1), total alkalinity (43.1 to 50.4mg/l), total dissolved solid (27.9 to 95.2 mg/l), total hardness (19.7 to 21.5mg/l), turbidity (12 to 170 NTU), and electrical conductivity (137.6 to 144.3 μmhos/cm). The result of the study indicated that the effluents from the fish pond could constitute a threat to the ecology of the aquatic water bodies if not properly treated before discharge.
Journal of Petroleum & Environmental Biotechnology, 2016
Background/Aim: Certain plasmids play an important role in adaptation of natural microbial popula... more Background/Aim: Certain plasmids play an important role in adaptation of natural microbial populations to oil and other hydrocarbons. This research investigated the role of plasmid-borne genes in the biodegradation of polycyclic aromatic hydrocarbons (PAHs) by a consortium of aerobic heterotrophic bacteria (AHB). Methods: Physicochemical and microbiological characteristics of the water sample were analyzed using standard methods. AHB with the capacity for biodegradation were isolated from hydrocarbon polluted water from Bodo Creek using Bushnell Haas Agar. The positive isolates were subjected to plasmid profiling and curing. Three treatments: cured, uncured and control were used in the study. Curing was achieved with acridine orange. The degradation experiment was carried out in a 10 L glass bioreactor and monitored for 56 days using GC-MS analysis. Results: Seven (7) out of 19 AHB isolates were selected for the degradation study. The 7 isolates, G1, G3, G19, GA5, GB3, GD1 and G12 were classified as Shewanella haliotis G1, Shewanella sp. G3, Vibrio alginolyticus G19, Pseudomonas putida GA5, Bacillus cereus GB3, B. pumilus GD1 and Shewanella sp. G12, respectively based on the phylogenetic analysis of their 16S rRNA genes. The sequences were deposited at the GenBank under the accession numbers KT886070-KT886076. The day 0 PAHs for cured, uncured and control treatments was 61.83 mg/L. After 56 days, the PAHs decreased to 2.90, 1.87 and 57.65 mg/L for cured, uncured and control treatments, respectively; representing percentage degradation of 95.31, 96.98 and 6.76%. The respective derived PAH degradation models for cured, uncured and control treatments were 32.614e-0.047t , 30.09e-0.05t and-0.0769t + 61.656. There was no significant difference (p<0.05) between PAH degradation in cured and uncured treatments. Conclusion: The study demonstrated that PAH degradation by the consortium used in the study was chromosomal and not plasmid-borne.
Asian Journal of Biotechnology and Bioresource Technology
Background: Production of amylase by Enterobacter cloacae D1 was optimized in this study using ce... more Background: Production of amylase by Enterobacter cloacae D1 was optimized in this study using central composite design (CCD) of response surface methodology (RSM). Methodology: Effects of five numeric factors (pH, temperature, inoculum concentration, peptone and yeast extract) on the production of amylase were examined. Amylase production was first screened using plate technique and amylase assay thereafter carried out using the dinitrosalicylic acid (DNSA) method. The CCD-RSM experimental set-up involved 30 runs with 5 levels of independent variables. Results: The amylase-producing bacterium Enterobacter cloacae strain D1 was identified based on the phylogenetic tree analysis of its sequence. The sequence has been submitted to GenBank under the accession number: MZ477010. The isolate had 98% similarity to the GenBank match Enterobacter cloacae strain ATCC 13182. Optimum conditions that yielded maximum amylase (34.43 U/mL) were pH 5; temperature 40 ℃; inoculum concentration 3%; pep...
Journal of Applied Life Sciences International, 2021
Background: Bacterial proteases represent a group of very important industrial enzymes. They are ... more Background: Bacterial proteases represent a group of very important industrial enzymes. They are involved in the hydrolysis of peptide bond found in protein. Industrial application of bacterial proteases has been limited by low yield and instability at biotechnological process conditions. This study was design to investigate the effect of temperature and pH on the stability of protease produced by Alcaligene faecalis strain P2. Methodology: Protease-producing bacteria were isolated from beans effluent-impacted soil and screened for protease production on Casein agar plate. Protease assay was carried out following standard method for protease determination and the stability of the protease produced was investigated over temperature range of 20 to 90 oC and pH range of 3 to 12. The protease-producing bacterium was identified using its molecular characteristics. Results: Protease assay result showed that the amount of tyrosine released by one unit of the crude enzyme was 0.176 µmol/mL ...
Biocatalysis and Agricultural Biotechnology
Journal of Advances in Microbiology
Aim: This study reports the production and optimization of cellulase from Penicillium sp. using c... more Aim: This study reports the production and optimization of cellulase from Penicillium sp. using corn-cob (CC) and pawpaw fibre (PF) as substrates. Methods: Nine fungal isolates, obtained from compost soil, were screened for cellulolytic activity. Isolate CPF-1, based on its ability to give the highest zones of clearance and cellulolytic activity, was selected. CPF-1 was identified as Penicillium sp. based on its cultural and morphological characteristics. Cellulase activity was determined by the DNS method on Congo red agar plate. Effects of temperature, pH and metal ions (Zn 2+ , Hg 2+ , Fe 2+ , Mg 2+ , Ca 2+ and Co 2+) on crude cellulase activity and stability were studied using two substrates (corn-cob and pawpaw fibre) by solid state fermentation.
Biotechnology Journal International
Aim: Viability of hydrocarbon-degrading bacterial consortium immobilized on different carriers wa... more Aim: Viability of hydrocarbon-degrading bacterial consortium immobilized on different carriers was studied. Methodology: Hydrocarbon-degrading bacteria were isolated from crude oil contaminated sites in Gio and K-Dera, Rivers State, Nigeria using enrichment method. Proximate analyses were carried out on the best carrier materials. Immobilization was by direct adsorption of the isolates onto the carrier materials and viability was determined by plate count method. The carrier materials tested included soya bran, sugarcane bagasse, corn cob, brown saw dust, white saw dust, cassava peel and red mud (bentonite). Results: The bacterial isolates demonstrated varied degradation capacity. The best carrier material was saw dust (103.6% survival) and corn cob (103.6% survival) followed by soya bran (94.4% survival rate) and cassava peel (94.4% survival rate). The saw dust had moisture content, 5.92%; ash content, 7.49%; crude protein, 2.2%; volatile matter, 74.28; and fixed carbon, 12.34%; wh...
Microbiology Research Journal International
Aims: To assess the quality of abattoir effluents discharged into water bodies in Owerri Municipa... more Aims: To assess the quality of abattoir effluents discharged into water bodies in Owerri Municipal, Nigeria using microbiological and physicochemical approaches. Study Design: The study employed microbiological and physicochemical parameters to determine effluent and water quality. Place and Duration of Study: Abattoirs in Owerri, Imo State, Nigeria, between September 2014 and February 2016. Methodology: Physicochemical and microbiological analyses were carried out on three abattoir effluents and their receiving water bodies. Counts of total heterotrophic bacteria, total coliform and faecal coliform, Vibrio, Salmonella and Shigella were carried using the plate count method. Results: The bacterial isolates in the various samples included members of the genera Bacillus, Citrobacter, Enterobacter, Escherichia, Klebsiella, Lactobacillus, Listeria, Micrococcus, Proteus, Salmonella, Serratia, Staphylococcus, Streptococcus and Vibrio. The order of increasing effluent’s total coliform and ...
Journal of Advances in Microbiology
Bioresources and Bioprocessing, 2016
Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria i... more Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria isolated from agrowaste-impacted soil through simultaneous saccharification and co-fermentation (SSCF) of steam-exploded bagasse was investigated. Methods: The cellulolytic (VCE-19) and xylanolytic (VXE-41) isolates were screened using the Congo Red Plate Method. The DNS method was used in the determination of reducing sugar content. Chemical analysis of the sugarcane bagasse was determined using standard methods. The bagasse was subjected to steam explosion to reduce lignin content and enhance cellulose availability. Results: Mean proximate composition analysis of the bagasse showed total carbohydrate and lignin content (% dry weight) of 70.3 ± 1.9 and 19.2 ± 1.2 before pretreatment and 85.4 ± 2.33 and 4.2 ± 0.44 after pretreatment, respectively. Phylogenetic analysis based on partial sequence of the 16S rRNA gene classified VCE-19 and VXE-41 as Bacillus cereus GBPS9 and Bacillus thuringiensis serovar kurstaki HD1, respectively. The sequences obtained from these isolates have been submitted to GenBank and accession numbers (KT350986.1 for VXE-41 and KT318371.1 for VCE-19) assigned to them. The result of the optimization of cultural conditions of the bacterial co-culture revealed optimum cellulase production at the following conditions: temperature, 40 °C; pH, 7; substrate concentration, 4.0 % (w/v); inoculum concentration, 4 % (v/v) and when yeast extract was used as nitrogen source. The gas chromatography-mass spectrometry (GC-MS) analysis of the fermentation broth detected the following components: acetone (3.49 g/L), ethylacetate (8.75 g/L), ethanol (19.08 g/L), N-propanol (4.96 g/L), isobutanol (3.73 g/L) and acetic acid (6.53 g/L). Conclusions: This study has demonstrated the production of significant quantity of ethanol by a co-culture of B. cereus GBPS9 and B. thuringiensis serovar kurstaki HD1 through SSCF of steam-exploded bagasse. Efficient bioethanol production from bagasse can help solve the need for alternative source of energy and the crisis that results from bioethanol production from food and feed crops.
Journal of Biotechnology & Biomaterials, 2015
Bioresources and Bioprocessing, May 26, 2016
Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria i... more Background: The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria isolated from agrowaste-impacted soil through simultaneous saccharification and co-fermentation (SSCF) of steam-exploded bagasse was investigated. Methods: The cellulolytic (VCE-19) and xylanolytic (VXE-41) isolates were screened using the Congo Red Plate Method. The DNS method was used in the determination of reducing sugar content. Chemical analysis of the sugarcane bagasse was determined using standard methods. The bagasse was subjected to steam explosion to reduce lignin content and enhance cellulose availability. Results: Mean proximate composition analysis of the bagasse showed total carbohydrate and lignin content (% dry weight) of 70.3 ± 1.9 and 19.2 ± 1.2 before pretreatment and 85.4 ± 2.33 and 4.2 ± 0.44 after pretreatment, respectively. Phylogenetic analysis based on partial sequence of the 16S rRNA gene classified VCE-19 and VXE-41 as Bacillus cereus GBPS9 and Bacillus thuringiensis serovar kurstaki HD1, respectively. The sequences obtained from these isolates have been submitted to GenBank and accession numbers (KT350986.1 for VXE-41 and KT318371.1 for VCE-19) assigned to them. The result of the optimization of cultural conditions of the bacterial co-culture revealed optimum cellulase production at the following conditions: temperature, 40 °C; pH, 7; substrate concentration, 4.0 % (w/v); inoculum concentration, 4 % (v/v) and when yeast extract was used as nitrogen source. The gas chromatography-mass spectrometry (GC-MS) analysis of the fermentation broth detected the following components: acetone (3.49 g/L), ethylacetate (8.75 g/L), ethanol (19.08 g/L), N-propanol (4.96 g/L), isobutanol (3.73 g/L) and acetic acid (6.53 g/L). Conclusions: This study has demonstrated the production of significant quantity of ethanol by a co-culture of B. cereus GBPS9 and B. thuringiensis serovar kurstaki HD1 through SSCF of steam-exploded bagasse. Efficient bioethanol production from bagasse can help solve the need for alternative source of energy and the crisis that results from bioethanol production from food and feed crops.
Microbiology Research Journal International
Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfa... more Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfactant by a hydrocarbon-utilizing bacterium, Stenotrophomonas sp. Methodology: The hydrocarbon-utilizing bacterium was isolated with Bushnell Haas (BH) broth using enrichment method. Biosurfactant production was screened by evaluating the following characteristics: Emulsification index (E-24), oil spreading (displacement), tilted glass slide, haemolysis on blood agar, and lipase production. Effects of combination of nitrogen sources (yeast extract and NH4NO3, yeast extract and urea, yeast extract and asparagine, yeast extract and peptone, NaNO3 and peptone, NaNO3 and asparagine, and yeast extract and NaNO3) and carbon sources (glucose, fructose, galactose, cassava peel, soya bran, olive oil, sucrose, crude oil, diesel and glycerol) on biosurfactant production were determined with emulsion stability and surface tension as responses. The bacterium was identified based on phenotypic, microsc...
Microbiology Research Journal International
Aim: This study reports the production of cellulase by Bacillus licheniformis VVA21 isolated from... more Aim: This study reports the production of cellulase by Bacillus licheniformis VVA21 isolated from hydrocarbon contaminated Kegbara-Dere mangrove in Ogoniland, Nigeria. Methodology: Baseline physicochemical characteristics of the hydrocarbon contaminated soil were established. Twenty-two bacterial isolates were screened for cellulolytic activity on carboxymethyl cellulose (CMC) agar using the spread plate technique. The isolate with the highest zone of clearance was selected and assayed further. Crude cellulase was extracted and partial purification achieved by ammonium sulphate precipitation, followed by dialysis, and final purification Original Research Article