Vinod Gubbiveeranna - Academia.edu (original) (raw)
Papers by Vinod Gubbiveeranna
Inorganic and Nano-Metal Chemistry
International Journal of Botany Studies, 2020
International Journal of Botany Studies, 2021
Gels, 2021
Copper-substituted nickel manganites Ni(1−x)CuxMn2O4 (Ni-TCE-NPs) were produced by co-precipitati... more Copper-substituted nickel manganites Ni(1−x)CuxMn2O4 (Ni-TCE-NPs) were produced by co-precipitation route (sol–gel) at room temperature. Ni(1−x)CuxMn2O4-Bio (NCB) NPs were studied by powder X-ray diffraction technique, scanning electron microscopy and Raman spectroscopy. XRD spectra authenticated the copper-doped nickel manganites’ formation with particle size 23–28 nm. A significant decrease in the lattice parameter confirmed the doping of copper ions into the nickel manganites. Microscopy (SEM) was used to estimate the grain size, shape and uniformity, revealing the non-uniform agglomerated polygon and plate-like microstructure. The NCB-NPs showed anticoagulant activity by enhancing the coagulation time of citrated plasma of human beings. NCB-NPs with x = 0.35 and 0.45 have increased clotting time from control 133 ± 4 s to 401 ± 7 s and 3554 ± 80 s, respectively, and others around 134 s. Additionally NCB-NPs with x = 0.35, 0.45 inhibited the platelet aggregation by 80% and 92%, wh...
Journal of Ethnopharmacology, 2021
ETHNOPHARMACOLOGICAL RELEVANCE In folk medicine, parts of Plumeria alba L. are used for treatment... more ETHNOPHARMACOLOGICAL RELEVANCE In folk medicine, parts of Plumeria alba L. are used for treatment of many diseases, with its latex being used for curing skin diseases and promoting wound healing. AIM OF THE STUDY This study aimed to study the role of P. alba L. latex in hemostasis and platelet aggregation. MATERIALS AND METHODS The latex of P. alba L. was processed to remove waxes and enrich protein content, and the final extract was named Plumeria alba L. natant latex (PaNL). PaNL was analyzed for protease activity against casein. The type of protease in PaNL was identified by using protease inhibitors such as E-64, phenylmethylsulfonyl fluoride, ethylenediaminetetraacetic acid, and pepstatin A. Human fibrinogen, fibrin, and collagen types I and IV were subjected to hydrolysis with different concentrations of PaNL. The thrombin-like activity of PaNL was determined by analyzing its fibrinogen-clotting and procoagulant activities. The role of PaNL in platelet aggregation was also investigated. Its hemorrhagic and edema-inducing activities were evaluated in a mouse model. Phytochemical compounds were identified by gas chromatography - mass spectroscopy. RESULTS The findings of casein/gelatin zymography confirmed that PaNL possesses protease activity. The results of the protease inhibition study indicated the presence of a cysteine-type protease(s) in PaNL. PaNL hydrolyzed the subunits of fibrinogen, fibrin, and collagen types I and IV. Its fibrin-degradation activity indicated that PaNL possesses plasmin-like activity. PaNL induced clotting of citrated human plasma within 3 min of incubation in the absence of CaCl2, indicating the presence of thrombin-like activity, which was further confirmed by the results of the fibrinogen-clotting assay. PaNL induced platelet aggregation in the absence of agonists. There was no hemolytic activity. Mice injected with PaNL did not show edema, or hemorrhagic activity. CONCLUSION PaNL possesses procoagulant, fibrino(geno)lytic, thrombin- and plasmin-like activities and induces platelet aggregation, which could explain its usage for wound treatment in folk medicine.
Journal of Pharmacognosy and Phytochemistry, 2019
The Jatropha curcas plant has been used since ancient time to treat open wounds, haemorrhage, to ... more The Jatropha curcas plant has been used since ancient time to treat open wounds, haemorrhage, to stop blood loss and to enhance wound healing process in folklore/tribal medicine by the people of tropical, subtropical and semi-arid countries. Discovery of new therapeutic molecule(s) is necessary to treat the thrombotic and other disorders in coagulation. There is a need to evaluate and isolate active molecules with medicinal property. This study is conducted to evaluate and characterize the Jatropha curcas plant latex for its fibrinogen lytic, collagenolytic and its action on blood coagulation cascade to substantiate the use of this plant latex as antithrombotic medicine.
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Tridax procumbens is a plant used majorly in Indian traditional medicine. This is rich in alkaloi... more Tridax procumbens is a plant used majorly in Indian traditional medicine. This is rich in alkaloids, steroids, carotenoids, flavonoids (such as catechins, centaurein and bergenins), fatty acids, phytosterols, tannins and minerals. Concoctions of extracts from T. procumbens leaves, stem, flower, and roots are used to treat patients suffering from diabetes, arthritis, inflammatory reactions and even applied to open wounds. The medicinal value of extracts has been evident by in vitro / in vivo assay of antioxidant, anti-bacterial, anti-inflammatory, anti-microbial, vasorelaxant, anti-leishmanial and mosquitocidal activities. Still, there is derth in the studies on isolation, characterization and evaluation of active principles from the extracts. This current review article gives comprehensive information about the T. procumbens taxonomy, morphology, geographical distribution, phytoconstituents and pharmacological activities.
Pharmacognosy Research, 2019
Background: Tridax procumbens extract (TPE) has been widely used in tribal/folk medicine to stop ... more Background: Tridax procumbens extract (TPE) has been widely used in tribal/folk medicine to stop bleeding and to enhance wound healing process. Based on its traditional medicinal importance, the TPE is evaluated for its possible role in blood coagulation cascade. Objective: This study has been conducted to evaluate the TPE for the presence of protease associated with fibrino (geno) lytic, collagenolytic properties, and its action on blood coagulation cascade and platelet aggregation to substantiate its procoagulant nature. Materials and Methods: The TPE was analyzed in vitro for proteolytic, fibrinogenolytic, and collagenolytic activity. It was also analyzed for its effect on blood coagulation and platelet aggregation. In vivo studies have been conducted for hemorrhagic and edema inducing activity. Results: A non-toxic serine protease having procoagulant property associated with fibrino (geno) lytic, collagenolytic (both type I and type IV), and platelet aggregation inducing was identified and evaluated from TPE. The TPE decreased the clotting time of human plasma as evaluated by recalcification time and partial thromboplastin time by 19.8 and 1.53 folds, respectively. This suggests its procoagulant nature. TPE also enhanced the adenosine diphosphate-/epinephrine-induced platelet aggregation by 1.35 and 1.38 folds, respectively. Conclusion: The TPE serine protease is a non-toxic procoagulant with fibrino (geno) lytic and collagenolytic activities and induces platelet aggregation. Further, isolation and characterization of active molecule in TPE will allow us to exploit the pharmacological potential of TPE on coagulation cascade.
Journal of Cluster Science, 2019
Objective: Investigate and describe the production, collection and disposal of residues of health... more Objective: Investigate and describe the production, collection and disposal of residues of health services (RHS) and characterize the exposure of Oncology staff from Cape Verde Hospital. Method: A descriptive study with quantitative and qualitative methodology, using semi-structured questionnaire, chek list and on-site observations to investigate the situation of the solid health waste handling and its risks. The participants in this study were professionals from a hospital who handle solid chemotherapy waste, in total 50 participants. Results: The data point to the lack of training of professionals exposing them to risks of occupational accidents, inadequate handling of RHS in the oncology outpatient clinic and to serious socio-environmental risks. Conclusion: Professionals, patients, general population and the environment are exposed to risks because of lack of training and an appropriate management plan of RHS.
Narasimhamurthy Konappa1‡, Udayashankar C Arakere1‡, Soumya Krishnamurthy2, Kusuma Chathrapalya G... more Narasimhamurthy Konappa1‡, Udayashankar C Arakere1‡, Soumya Krishnamurthy2, Kusuma Chathrapalya Gangadharaiah3, Vinod Gubbiveeranna3, Nagaraju Shivaiah3, Srinivas Chowdappa4 & Niranjana Siddapura Ramachandrappa*1 1Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India 2Field Marshal K.M. Cariappa College, A Constituent College of Mangalore University, Madikeri 571 201, Karnataka, India 3Department of Studies and Research in Biochemistry, Tumkur University, Tumkur 572 103, Karnataka, India 4Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bangalore 560 056, Karnataka, India ‡Contributed equally *Email: niranjanasr@rediff.com
Plant Science Today
Manilkara zapota (L.) P. Royen (Sapotaceae), is widely used in traditional medicine for various a... more Manilkara zapota (L.) P. Royen (Sapotaceae), is widely used in traditional medicine for various ailments like, diarrhea, pulmonary diseases, piles, ulcers and to treat wounds. The present study evaluates the role of M. zapota latex in hemostasis. The processed latex named as M. zapota natant latex (MzNL), has proteins at the concentration of 8 mg/ml and showed protein bands in Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The proteolytic activity of MzNL was evaluated using casein in comparison with trypsin. The phenylmethylsulfonyl fluoride (PMSF) inhibited the protease activity indicating the possible presence of serine protease. The effect of temperature, pH and metal ions on proteolytic activity was evaluated. MzNL exhibited fibrinogenolytic activity by hydrolysing A? and B? subunits of fibrinogen. However, ? subunit remained resistant for hydrolysis. MzNL hydrolyzed all the subunits of collagen type I and IV at the concentration of 8 µg and 25 µg in 20 µ...
Inorganic and Nano-Metal Chemistry
International Journal of Botany Studies, 2020
International Journal of Botany Studies, 2021
Gels, 2021
Copper-substituted nickel manganites Ni(1−x)CuxMn2O4 (Ni-TCE-NPs) were produced by co-precipitati... more Copper-substituted nickel manganites Ni(1−x)CuxMn2O4 (Ni-TCE-NPs) were produced by co-precipitation route (sol–gel) at room temperature. Ni(1−x)CuxMn2O4-Bio (NCB) NPs were studied by powder X-ray diffraction technique, scanning electron microscopy and Raman spectroscopy. XRD spectra authenticated the copper-doped nickel manganites’ formation with particle size 23–28 nm. A significant decrease in the lattice parameter confirmed the doping of copper ions into the nickel manganites. Microscopy (SEM) was used to estimate the grain size, shape and uniformity, revealing the non-uniform agglomerated polygon and plate-like microstructure. The NCB-NPs showed anticoagulant activity by enhancing the coagulation time of citrated plasma of human beings. NCB-NPs with x = 0.35 and 0.45 have increased clotting time from control 133 ± 4 s to 401 ± 7 s and 3554 ± 80 s, respectively, and others around 134 s. Additionally NCB-NPs with x = 0.35, 0.45 inhibited the platelet aggregation by 80% and 92%, wh...
Journal of Ethnopharmacology, 2021
ETHNOPHARMACOLOGICAL RELEVANCE In folk medicine, parts of Plumeria alba L. are used for treatment... more ETHNOPHARMACOLOGICAL RELEVANCE In folk medicine, parts of Plumeria alba L. are used for treatment of many diseases, with its latex being used for curing skin diseases and promoting wound healing. AIM OF THE STUDY This study aimed to study the role of P. alba L. latex in hemostasis and platelet aggregation. MATERIALS AND METHODS The latex of P. alba L. was processed to remove waxes and enrich protein content, and the final extract was named Plumeria alba L. natant latex (PaNL). PaNL was analyzed for protease activity against casein. The type of protease in PaNL was identified by using protease inhibitors such as E-64, phenylmethylsulfonyl fluoride, ethylenediaminetetraacetic acid, and pepstatin A. Human fibrinogen, fibrin, and collagen types I and IV were subjected to hydrolysis with different concentrations of PaNL. The thrombin-like activity of PaNL was determined by analyzing its fibrinogen-clotting and procoagulant activities. The role of PaNL in platelet aggregation was also investigated. Its hemorrhagic and edema-inducing activities were evaluated in a mouse model. Phytochemical compounds were identified by gas chromatography - mass spectroscopy. RESULTS The findings of casein/gelatin zymography confirmed that PaNL possesses protease activity. The results of the protease inhibition study indicated the presence of a cysteine-type protease(s) in PaNL. PaNL hydrolyzed the subunits of fibrinogen, fibrin, and collagen types I and IV. Its fibrin-degradation activity indicated that PaNL possesses plasmin-like activity. PaNL induced clotting of citrated human plasma within 3 min of incubation in the absence of CaCl2, indicating the presence of thrombin-like activity, which was further confirmed by the results of the fibrinogen-clotting assay. PaNL induced platelet aggregation in the absence of agonists. There was no hemolytic activity. Mice injected with PaNL did not show edema, or hemorrhagic activity. CONCLUSION PaNL possesses procoagulant, fibrino(geno)lytic, thrombin- and plasmin-like activities and induces platelet aggregation, which could explain its usage for wound treatment in folk medicine.
Journal of Pharmacognosy and Phytochemistry, 2019
The Jatropha curcas plant has been used since ancient time to treat open wounds, haemorrhage, to ... more The Jatropha curcas plant has been used since ancient time to treat open wounds, haemorrhage, to stop blood loss and to enhance wound healing process in folklore/tribal medicine by the people of tropical, subtropical and semi-arid countries. Discovery of new therapeutic molecule(s) is necessary to treat the thrombotic and other disorders in coagulation. There is a need to evaluate and isolate active molecules with medicinal property. This study is conducted to evaluate and characterize the Jatropha curcas plant latex for its fibrinogen lytic, collagenolytic and its action on blood coagulation cascade to substantiate the use of this plant latex as antithrombotic medicine.
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Tridax procumbens is a plant used majorly in Indian traditional medicine. This is rich in alkaloi... more Tridax procumbens is a plant used majorly in Indian traditional medicine. This is rich in alkaloids, steroids, carotenoids, flavonoids (such as catechins, centaurein and bergenins), fatty acids, phytosterols, tannins and minerals. Concoctions of extracts from T. procumbens leaves, stem, flower, and roots are used to treat patients suffering from diabetes, arthritis, inflammatory reactions and even applied to open wounds. The medicinal value of extracts has been evident by in vitro / in vivo assay of antioxidant, anti-bacterial, anti-inflammatory, anti-microbial, vasorelaxant, anti-leishmanial and mosquitocidal activities. Still, there is derth in the studies on isolation, characterization and evaluation of active principles from the extracts. This current review article gives comprehensive information about the T. procumbens taxonomy, morphology, geographical distribution, phytoconstituents and pharmacological activities.
Pharmacognosy Research, 2019
Background: Tridax procumbens extract (TPE) has been widely used in tribal/folk medicine to stop ... more Background: Tridax procumbens extract (TPE) has been widely used in tribal/folk medicine to stop bleeding and to enhance wound healing process. Based on its traditional medicinal importance, the TPE is evaluated for its possible role in blood coagulation cascade. Objective: This study has been conducted to evaluate the TPE for the presence of protease associated with fibrino (geno) lytic, collagenolytic properties, and its action on blood coagulation cascade and platelet aggregation to substantiate its procoagulant nature. Materials and Methods: The TPE was analyzed in vitro for proteolytic, fibrinogenolytic, and collagenolytic activity. It was also analyzed for its effect on blood coagulation and platelet aggregation. In vivo studies have been conducted for hemorrhagic and edema inducing activity. Results: A non-toxic serine protease having procoagulant property associated with fibrino (geno) lytic, collagenolytic (both type I and type IV), and platelet aggregation inducing was identified and evaluated from TPE. The TPE decreased the clotting time of human plasma as evaluated by recalcification time and partial thromboplastin time by 19.8 and 1.53 folds, respectively. This suggests its procoagulant nature. TPE also enhanced the adenosine diphosphate-/epinephrine-induced platelet aggregation by 1.35 and 1.38 folds, respectively. Conclusion: The TPE serine protease is a non-toxic procoagulant with fibrino (geno) lytic and collagenolytic activities and induces platelet aggregation. Further, isolation and characterization of active molecule in TPE will allow us to exploit the pharmacological potential of TPE on coagulation cascade.
Journal of Cluster Science, 2019
Objective: Investigate and describe the production, collection and disposal of residues of health... more Objective: Investigate and describe the production, collection and disposal of residues of health services (RHS) and characterize the exposure of Oncology staff from Cape Verde Hospital. Method: A descriptive study with quantitative and qualitative methodology, using semi-structured questionnaire, chek list and on-site observations to investigate the situation of the solid health waste handling and its risks. The participants in this study were professionals from a hospital who handle solid chemotherapy waste, in total 50 participants. Results: The data point to the lack of training of professionals exposing them to risks of occupational accidents, inadequate handling of RHS in the oncology outpatient clinic and to serious socio-environmental risks. Conclusion: Professionals, patients, general population and the environment are exposed to risks because of lack of training and an appropriate management plan of RHS.
Narasimhamurthy Konappa1‡, Udayashankar C Arakere1‡, Soumya Krishnamurthy2, Kusuma Chathrapalya G... more Narasimhamurthy Konappa1‡, Udayashankar C Arakere1‡, Soumya Krishnamurthy2, Kusuma Chathrapalya Gangadharaiah3, Vinod Gubbiveeranna3, Nagaraju Shivaiah3, Srinivas Chowdappa4 & Niranjana Siddapura Ramachandrappa*1 1Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India 2Field Marshal K.M. Cariappa College, A Constituent College of Mangalore University, Madikeri 571 201, Karnataka, India 3Department of Studies and Research in Biochemistry, Tumkur University, Tumkur 572 103, Karnataka, India 4Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bangalore 560 056, Karnataka, India ‡Contributed equally *Email: niranjanasr@rediff.com
Plant Science Today
Manilkara zapota (L.) P. Royen (Sapotaceae), is widely used in traditional medicine for various a... more Manilkara zapota (L.) P. Royen (Sapotaceae), is widely used in traditional medicine for various ailments like, diarrhea, pulmonary diseases, piles, ulcers and to treat wounds. The present study evaluates the role of M. zapota latex in hemostasis. The processed latex named as M. zapota natant latex (MzNL), has proteins at the concentration of 8 mg/ml and showed protein bands in Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The proteolytic activity of MzNL was evaluated using casein in comparison with trypsin. The phenylmethylsulfonyl fluoride (PMSF) inhibited the protease activity indicating the possible presence of serine protease. The effect of temperature, pH and metal ions on proteolytic activity was evaluated. MzNL exhibited fibrinogenolytic activity by hydrolysing A? and B? subunits of fibrinogen. However, ? subunit remained resistant for hydrolysis. MzNL hydrolyzed all the subunits of collagen type I and IV at the concentration of 8 µg and 25 µg in 20 µ...