Vladimir Sergeev - Academia.edu (original) (raw)
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Birla Institute of Technology and Sciences, Pilani
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Papers by Vladimir Sergeev
RSC Advances, 2019
Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is ... more Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases.
Biochemistry. Biokhimii͡a, 2000
Site-directed mutagenesis of the ecoRII gene has been used to search for the active site of the E... more Site-directed mutagenesis of the ecoRII gene has been used to search for the active site of the EcoRII restriction endonuclease. Plasmids with point mutations in ecoRII gene resulting in substitutions of amino acid residues in the Asp110-Glu112 region of the EcoRII endonuclease (Asp110 --> Lys, Asn, Thr, Val, or Ile; Pro111 --> Arg, His, Ala, or Leu; Glu112 --> Lys, Gln, or Asp) have been constructed. When expressed in E. coli, all these plasmids displayed EcoRII endonuclease activity. We also constructed a plasmid containing a mutant ecoRII gene with deletion of the sequence coding the Gln109-Pro111 region of the protein. This mutant protein had no EcoRII endonuclease activity. The data suggest that Asp110, Pro111, and Glu112 residues do not participate in the formation of the EcoRII active site. However, this region seems to be relevant for the formation of the tertiary structure of the EcoRII endonuclease.
Nucleic Acids Research, 1991
Chemical ligation of oligonucleotides in doublestranded helices has been considered in its struct... more Chemical ligation of oligonucleotides in doublestranded helices has been considered in its structuralkinetic aspect. A study was made of (i) two series of DNA duplexes with various arrangements of reacting groups in the ligation junction induced by mispairing or by alteration of furanose structure (the replacement of dT unit with rU, all, IU, xU, dxT ones) and of (ii) eight synthetic water-soluble carbodiimides with different substituents at N1 and N3 atoms. We assumed that some information on the local structure of modified sites in the duplex can be obtained from kinetic parameters of oligonucleotide coupling reaction. The ratio of kinetic constants k 3 /(k 2 + k 3) for productive and nonproductive decomposition of the activated phosphomonoester derivative apparently reflects the reaction site structure: for a given duplex this parameter is virtually independent of the condensing agent composition. Based on the analysis of the chemical ligation kinetics a suggestion has been made about the conformation of some modified units in the double helix.
RSC Advances, 2016
The present study relates to the development of cationic polymers that are of great interest due ... more The present study relates to the development of cationic polymers that are of great interest due to their enormous potential for biomedical applications, especially as non-viral vectors for gene therapy, active components in DNA sensing devices, etc.
RSC Advances, 2019
Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is ... more Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases.
Biochemistry. Biokhimii͡a, 2000
Site-directed mutagenesis of the ecoRII gene has been used to search for the active site of the E... more Site-directed mutagenesis of the ecoRII gene has been used to search for the active site of the EcoRII restriction endonuclease. Plasmids with point mutations in ecoRII gene resulting in substitutions of amino acid residues in the Asp110-Glu112 region of the EcoRII endonuclease (Asp110 --> Lys, Asn, Thr, Val, or Ile; Pro111 --> Arg, His, Ala, or Leu; Glu112 --> Lys, Gln, or Asp) have been constructed. When expressed in E. coli, all these plasmids displayed EcoRII endonuclease activity. We also constructed a plasmid containing a mutant ecoRII gene with deletion of the sequence coding the Gln109-Pro111 region of the protein. This mutant protein had no EcoRII endonuclease activity. The data suggest that Asp110, Pro111, and Glu112 residues do not participate in the formation of the EcoRII active site. However, this region seems to be relevant for the formation of the tertiary structure of the EcoRII endonuclease.
Nucleic Acids Research, 1991
Chemical ligation of oligonucleotides in doublestranded helices has been considered in its struct... more Chemical ligation of oligonucleotides in doublestranded helices has been considered in its structuralkinetic aspect. A study was made of (i) two series of DNA duplexes with various arrangements of reacting groups in the ligation junction induced by mispairing or by alteration of furanose structure (the replacement of dT unit with rU, all, IU, xU, dxT ones) and of (ii) eight synthetic water-soluble carbodiimides with different substituents at N1 and N3 atoms. We assumed that some information on the local structure of modified sites in the duplex can be obtained from kinetic parameters of oligonucleotide coupling reaction. The ratio of kinetic constants k 3 /(k 2 + k 3) for productive and nonproductive decomposition of the activated phosphomonoester derivative apparently reflects the reaction site structure: for a given duplex this parameter is virtually independent of the condensing agent composition. Based on the analysis of the chemical ligation kinetics a suggestion has been made about the conformation of some modified units in the double helix.
RSC Advances, 2016
The present study relates to the development of cationic polymers that are of great interest due ... more The present study relates to the development of cationic polymers that are of great interest due to their enormous potential for biomedical applications, especially as non-viral vectors for gene therapy, active components in DNA sensing devices, etc.