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Papers by WAHIDIN TEGUH SASONGKO

Research Square (Research Square), Apr 1, 2024

Highlight 1. A sugarcane-based diet enhances sheep productivity, particularly in terms of daily w... more Highlight 1. A sugarcane-based diet enhances sheep productivity, particularly in terms of daily weight gain and feed conversion. 2. Sheep fed on a sugarcane-based diet exhibit carcass characteristics with lower carcass weight and dressing percentage. 3. The protein and lactose concentration in sheep milk decreases due to a sugarcane-based diet.

Veterinary world/Veterinary World, Feb 1, 2024

Background and Aim: Irradiated chitosan can be used as a matrix for slow-release urea (SRU) produ... more Background and Aim: Irradiated chitosan can be used as a matrix for slow-release urea (SRU) production. This study aimed to (1) determine the optimal formulation of irradiated chitosan matrix for controlling nitrogen release and (2) evaluate the characteristics of SRU in vitro fermentation based on irradiated chitosan as a feed supplement. Materials and Methods: In the first phase of the investigation, four chitosan-based SRU formulations with varying amounts of acrylamide (3 and 5 g) and gamma irradiation (5 and 10 kGy) were evaluated. Scanning electron microscopy, Fourier transform mid-infrared spectroscopy, and ammonia release characteristics were used to observe morphological, functional group, and ammonia release characteristics. In the second phase of research, the most effective SRU formulation was utilized as a supplement to ruminant rations based on rice straw, sorghum straw, and alfalfa. Gas production, rumen fermentation characteristics, and methane gas production were observed in vitro. Results: On the basis of surface image analysis, the four SRU formulas generate a similar appearance. Compared with untreated urea, the SRU3 formula reduced the percentage of ammonia emission by 12.85%-27.64% after 24 h of incubation (p = 0.05), as determined by the first phase study. SRU3 became the basis for the second testing phase. The addition of SRU3 did not affect the optimal gas production in vitro. SRU3 treatment produced less gas than Optigen® treatment (p = 0.05). With regard to rumen fermentation and digestibility, Optigen® yielded better results than SRU3 (p = 0.05). However, the treatment with SRU3 resulted in reduced methane production compared to that in the control (p = 0.05). Conclusion: Irradiated chitosan as an SRU matrix may control the release of ammonia in the rumen medium. The SRU3 formulation is the most effective. The addition of SRU to rice straw-based rations reduces methane production without affecting in vitro digestibility.

AIP conference proceedings, 2024

Penerbit BRIN eBooks, Dec 28, 2023

BIO web of conferences, Dec 31, 2022

Penelitian ini bertujuan untuk mengetahui pengaruh UMMB dan 3 pakan suplemen lainnya terhadap kua... more Penelitian ini bertujuan untuk mengetahui pengaruh UMMB dan 3 pakan suplemen lainnya terhadap kualitas cairan rumen domba. Rancangan yang digunakan adalah rancangan Bujur Sangkar Latin 4 x 4, dengan 4 macam perlakuan pakan suplemen. Parameter yang diamati : pH, amonia, daya cerna terhadap bahan kering (BK) dan bahan organik (BO), serta biomasa sel dengan P32. Data penelitian menunjukkan bahwa pengaruh perlakuan tidak berpengaruh nyata terhadap pH cairan rumen, daya cerna terhadap BK dan BO, produksi gas, sintesis mikrobia maupun efisiensi pembentukan mikrobia. Perlakuan memberikan perbedaan yang nyata terhadap kadar amonia cairan rumen. Amonia cairan rumen tertinggi dicapai pada suplemen pakan formula baru yaitu 31.39 mg/100 ml diikuti UMMB, Nutrifeed dan Nutrifeed + UMMB berturut-turut 28,58; 27,95 dan 41mg/100ml. Dari penelitian ini dapat disimpulkan bahwa suplemen pakan formula baru mempunyai peluang untuk dikembangkan.

Telah dilakukan pengujian di lapang proteksi protein dengan menggunakan tanin yang berasal dari d... more Telah dilakukan pengujian di lapang proteksi protein dengan menggunakan tanin yang berasal dari daun nangka. Ternak yang digunakan berjum]ah 20 ekor sapi Peranakan Ongole (PO) umur rata-rata 2 tahun dan rerata bobot badan awal 212 ± 18 kg. Perlakuan pakan yang digunakan adalah Pl : kontrol (rumput lapangan); P2 : kontrol positif: rumput lapangan + kedelai; P3: rumput lapangan + kedelai + tanin 0,7%; P4 rumput lapangan + kedelai + tanin 1,4%. Masing-masing perlakuan terdiri dari 8 ekor sapi sebagai ulangan. Perubahan yang diamati adalah Pertambahan Bobot Badan Harian (PBBH). Perlakuan yang paling efisien yaitu dengan pemberian rumput lapangan + kedelai + tanin 0,7% dapat meningkatkan PBBH sebesar 0,86 kg/ekor/hari lebih tinggi dibandingkan dengan kontrol positif sebesar 94,19%.

Jurnal ilmiah aplikasi isotop dan radiasi : A Scientific journal for the applications of isotipes and radiation, Dec 16, 2017

Teknik produksi gas digunakan untuk mengetahui perbandingan beberapa jenis galur mutan sorgum yan... more Teknik produksi gas digunakan untuk mengetahui perbandingan beberapa jenis galur mutan sorgum yang dihasilkan oleh BATAN sebagai pakan ruminansia. 8 jenis daun galur mutan sorgum dan rancangan acak lengkap digunakan dalam pengujian ini. Untuk melihat perbandingan antara perlakuan dilakukan uji anova dan uji lanjut BNT bila pengaruh perlakuan signifikan. Sampel ditimbang 375 mg, dimasukkan ke dalam syringe glass 100 ml ditambah 30 ml media campuran cairan rumen dengan buffer bicarbonat dan diinkubasi pada suhu 39 o C selama 24 jam. Variabel yang diukur adalah produksi gas setelah 0, 2, 4, 6, 8,19, 12, 24 dan 48 jam inkubasi, potensi produksi gas, degradabilitas bahan kering (DBK) dan organik (DBO). Hasil yang diperoleh menunjukkan produksi gas, potensi produksi gas dan degradabilitas yang dihasilkan berbeda nyata (P<0,05). Produksi gas tertinggi setelah 24 dan 48 jam adalah perlakuan H dan B yaitu 57,59 dan 71,75 ml/375 mg BK, sementara itu terendah adalah perlakuan I dan G yaitu 50,33 dan 54,92 ml/375 mg BK. Potensi produksi gas yang dihasilkan tertinggi adalah perlakuan B yaitu 85,46 ml/375 mg BK dan terendah adalah perlakuan G yaitu 56,74 ml/375 mg BK, sementara itu persentase produksi gas selama 24 jam tertinggi adalah perlakuan G yaitu 82,45% dan terendah perlakuan B yaitu 62,24%. Degradabilitas bahan kering (DBK) tertinggi setelah 24 dan 48 jam inkubasi adalah perlakuan D dan A yaitu 54,45 dan 67,31%, sementara itu terendah adalah perlakuan G yaitu 50,85 dan 58,93%. Degradabilitas bahan organik (DBO) tertinggi tertinggi setelah 24 dan 48 jam inkubasi adalah perlakuan D dan B yaitu 54,13 dan 69,13%, sementara itu terendah adalah perlakuan G yaitu 50,67 dan 58,93%. Secara umum ada dua jenis galur mutan sorgum hasil iradiasi yaitu mudah terdegradasi sebelum 24 jam dan setelah 24 jam, sehingga bisa digunakan untuk pedoman pemberian pakan untuk ruminansia.

Buletin Fakultas Peternakan Universitas Gadjah Mada, Feb 27, 2017

Advances in Animal and Veterinary Sciences, 2022

Our objective was to investigate the effect of different variety and wilting treatment on silage ... more Our objective was to investigate the effect of different variety and wilting treatment on silage quality and in vitro degradability of whole-plant sorghum. Three sorghum varieties (Numbu, Super 1 and Samurai 1) were ensiled either fresh or wilted and evaluated in a 2 x 3 factorial arrangement. Based on sensory evaluation, colour, smell and sensory index increased after wilting treatment (P < 0.01). Based on chemical quality, pH and NH 3-N values were lower in wilted groups than in unwilting sorghum silage (P < 0.01). Compared with non-wilted materials, higher dry-matter (DM) and organic-matter (OM) content were found in wilted materials (P < 0.01). Wilting did not affect crude protein (CP), ether extract (EE), neutral detergent fibre (NDF), acid detergent fibre (ADF), hemicellulose, cellulose, or non-fibre carbohydrate (NFC) content. Samurai 1 sorghum silage had the lowest NDF and ADF, both in non-wilted and wilted materials (P < 0.05). The interaction of wilting and different variety had a significant impact on NDF (P < 0.05), ADF, OM, and CP (P < 0.01). Wilting treatment had no significant impact on all aspects of in vitro degradability. In contrast, variety difference had a significant impact on in vitro degradability (P < 0.01). Results of the current study indicate that wilting treatment influences the sensory score and chemical quality of sorghum silage. There was no effect on nutrient composition or in vitro digestibility. The effect of different variety on the nutrient value of sorghum silage was more pronounced than the wilting variable.

Advances in Animal and Veterinary Sciences, 2022

This work investigates the effects of different soil pH and different genotypes on the nutrient p... more This work investigates the effects of different soil pH and different genotypes on the nutrient profiles and in vitro digestibility of soybeans. Thirty soybean genotypes were evaluated for nutrient composition, fiber content, and in vitro digestibility after cultivation in pH 4.0 and 5.4 soils. The experiment was arranged factorially in a completely randomized block design. The experimental factors were soybean genotype and soil pH. Results showed significant (p < 0.001) differences in fiber content, non-fiber carbohydrate content, and in vitro dry matter digestibility (IVDMD) parameters among studied genotypes. There were significant differences (p < 0.05) between the two soil pH conditions for ash, organic matter, ether extract, neutral detergent fiber, acid detergent fiber, hemicellulose, and nonfiber carbohydrate. Organic matter, crude protein, ether extract, and non-fiber carbohydrate content in soil with pH 5.4

IOP conference series, Nov 1, 2021

In vitro and in vivo testing for ruminant feed efficiency can be done by utilizing the stable iso... more In vitro and in vivo testing for ruminant feed efficiency can be done by utilizing the stable isotope Nitrogen-15 (15N) as a tracer. Feed can be traced by labeling the forage using isotope 15N. Feed crops are labeled using an isotope 15N-enriched fertilizer. The critical thing to note is to know the content of isotopes 15N in the part of forage feed plants that have been labeled. This research aims to know the effect of urea fertilizer on the percent of atom excess 15N on corn. Corn are labeled using urea enriched with isotopes 15N in the form of urea fertilizer (10% excess atom 15N) with different doses (0-200% recommended urea dose). As a control used corn plants given urea fertilizer is not labeled 15N. The results showed that corn forage feed was successfully labeled and correlated with the dose of fertilizer. The range of atom excess 15N was 4.28 – 6.99% in corn forage. Biomass production showed no significant difference between the dose of fertilization and control, but neither protein content. Based on data, the corn forage can be used for further testing.

Journal of animal health and production, 2021

This study investigated the effect of harvesting time on concentration and biological activity of... more This study investigated the effect of harvesting time on concentration and biological activity of tannin in Andrographis paniculata (A. paniculata) leaves and determined the in vitro rumen fermentation and digestibility of diet supplemented with A. paniculata leaves to evaluate its role as herbal supplement in ruminant's feed. A. paniculata leaves were harvested at two time points i.e., before and after flowering phase and the samples were dried to analyze concentration and biological activity of tannin. Rice straw was used as basal diet to carry out in vitro digestibility trial. Four treatments were used including B consisting basal diet only, BBF and BAF each consisting basal diet supplemented with 1% A. paniculata leaves before and after flowering phases, respectively, and BBAF supplemented with 0.5% A. paniculata leaves before and 0.5% after flowering phase. Each treatment was carried out in five replications. Results revealed that contents (mg/g) for total tannin (20.40±1.06 vs 19.33±1.19; P<0.05) and condensed tannin (8.44±0.17 vs 5.47±0.30; P<0.01) were greater in leaves harvested after flowering compared with before flowering phase, however, no significant difference (P > 0.05) on biological activity of tannins was observed between the two flowering phases. The in vitro gas production was not influenced (P > 0.05) by the supplementation of A. paniculata leaves before and after flowering phase. A. paniculata supplementation also did not influence (P > 0.05) in vitro organic matter digestibility (IVOMD) and total volatile fatty acids (TVFA) values. It was concluded that the supplementation of A. paniculata leaves before and after flowering phase in the diet does not affect the digestibility.

Advances in Animal and Veterinary Sciences, 2020

Five rice straw cultivars in Indonesia (Atomita 1, Bestari, Inpari Sidenuk, Situ Gintung and Cihe... more Five rice straw cultivars in Indonesia (Atomita 1, Bestari, Inpari Sidenuk, Situ Gintung and Ciherang) were investigated for nutrient and in vitro digestibility as ruminants roughage. Except Ciherang, all cultivars were mutant rice variety. This study aimed to: 1) assess the influence of variety on the nutrient and fiber variation of rice straw; 2) predict the nutrient value of rice straw using fiber content; and 3) evaluate the in vitro digestibility of five rice straw cultivars in Indonesia. Except for acid detergent lignin (ADL) (P= 0.09), a significant difference (P<0.05) were observed for all nutrient and fiber contents between all varieties. Acid detergent fiber (ADF) and cellulose content in mutant varieties were significantly higher than Ciherang variety. Based on fiber content, the range in relative feed value (RFV) varied by 60.99-68.89. However, all rice straw varieties are included in reject forage class. There were significant differences at 48 and 72 h in vitro gas production (P<0.05) between all varieties. Highly significant differences (P<0.001) were observed for optimum (a+b) and rate gas production (c) traits. The in vitro organic matter digestibility (IVOMD) varied from 30.30-35.87%. Those results could explain differences in nutritional quality and digestibility of rice straw according to cultivars. Ciherang variety had a good prospect for ruminant roughage due to the highest nutrient value and digestibility.

IOP conference series, Jun 1, 2019

Livestock, as part of agriculture sector, has contribute to greenhouse gas (GHG) emission especia... more Livestock, as part of agriculture sector, has contribute to greenhouse gas (GHG) emission especially on methane (CH4) compounds. Methane emission represent energy losses in form of gas reflecting low feed efficiency. Rice straw is a source commonly used as animal roughage. The quality of rice straw could be improved by fermentation treatment. This study investigated effects of fermentation using Phanerochaete chrysosporium and Trichoderma viride inoculant on in vitro gas and methane emission from rice straw. The rice straw was fermented in three weeks. This experiment consisted of four treatments and three replications being: (1) C (control, fermented rice straw with no inoculant), (2) TV (fermented rice straw using T. viride inoculant), (3) PC (fermented rice straw using P. chrysosporium inoculant), (4) TVPC (fermented rice straw using T. viride and P. chrysosporium inoculant). This research was arranged into a completely randomized block design. Results showed that TVPC treatment produced the lowest lignin content (p<0.05). TVPC treatment also produced the highest glucose compound by 1.70 mg/g (p<0.05). Meanwhile, there was no difference between all treatments in in vitro total gas and optimum gas (a+b) production. However, adding T. viride combination with P. chrysosporium in fermented process could decrease methane gas production (P<0.01). Results demonstrate that adding T. viride and P. chrysosporium inoculant in fermented process could increase the efficiency of rice straw as ruminant's feed. This was represent in the low methane production and could reduce a part of GHG emission from enteric fermentation.

Livestock and Animal Research, Nov 26, 2020

Penelitian ini bertujuan untuk mengevaluasi pengaruh iradiasi gamma terhadap kandungan nutrien, f... more Penelitian ini bertujuan untuk mengevaluasi pengaruh iradiasi gamma terhadap kandungan nutrien, fenol dan aktivitas biologis tanin daun nangka (Artocarpus heterophyllus). Aktivitas biologis yang dimaksud adalah kemampuan tanin dalam mengikat protein yang terkandung dalam substrat pakan. Metode: Perlakuan penelitian adalah: DN (daun nangka tanpa iradiasi gamma); DN 5 (daun nangka yang diiradiasi gamma pada dosis 5 kGy); DN 7,5 (daun nangka yang diiradiasi gamma pada dosis 7,5 kGy) dan DN 10 (daun nangka yang diiradiasi gamma pada dosis 10 kGy). Variabel yang diamati adalah profil nutrien, fraksi serat, anti nutrien dan aktivitas biologis tanin. Pada pengujian aktivitas biologis tanin, keempat perlakuan ditambahkan polietilen glikol (PEG) sebagai agen inaktivasi tanin. Rancangan Acak Lengkap (RAL) dengan lima ulangan digunakan dalam penelitian ini. Hasil: Hasil penelitian menunjukkan bahwa iradiasi gamma tidak memberikan pengaruh yang nyata terhadap kandungan bahan organik (BO), protein kasar (PK), acid detergent fiber (ADF), total phenol dan total tanin pada daun nangka. Iradiasi gamma justru menurunkan aktivitas biologis tanin yang direpresentasikan oleh produksi gas kumulatif in vitro. Walaupun demikian, dosis iradiasi gamma 5; 7,5 dan 10 kGy mampu menurunkan kandungan lemak kasar (LK) daun nangka berturut-turut sebesar 57,82; 83,64 dan 97,09% dari kandungan awal. Kesimpulan: Kesimpulan penelitian ini adalah Aktivitas biologis tanin pada daun nangka menurun setelah diiradiasi gamma pada dosis > 5 kGy. Dosis iradiasi gamma 7,5 kGy mampu meningkatkan produksi gas komulatif dan kecernaan bahan organik in vitro daun nangka.

Advances in Animal and Veterinary Sciences, 2021

The comparison was made between eighteen mutant and four non-mutant rice straw variety in Indones... more The comparison was made between eighteen mutant and four non-mutant rice straw variety in Indonesia in terms of chemical composition, nutrient value and in vitro digestibility. The second objective was to determine the correlation between in vitro digestibility parameters and fibrous component in twenty-two Indonesian rice straw varieties. Rice straw samples were collected in triplicate from three replicate plots. The effect of variety on rice straw quality, nutrient values and in vitro digestibility was analyzed using one-way analysis of variance (ANOVA). Results demonstrated that the rice straw varieties differed (P<0.

Research Square (Research Square), Apr 1, 2024

Highlight 1. A sugarcane-based diet enhances sheep productivity, particularly in terms of daily w... more Highlight 1. A sugarcane-based diet enhances sheep productivity, particularly in terms of daily weight gain and feed conversion. 2. Sheep fed on a sugarcane-based diet exhibit carcass characteristics with lower carcass weight and dressing percentage. 3. The protein and lactose concentration in sheep milk decreases due to a sugarcane-based diet.

Veterinary world/Veterinary World, Feb 1, 2024

Background and Aim: Irradiated chitosan can be used as a matrix for slow-release urea (SRU) produ... more Background and Aim: Irradiated chitosan can be used as a matrix for slow-release urea (SRU) production. This study aimed to (1) determine the optimal formulation of irradiated chitosan matrix for controlling nitrogen release and (2) evaluate the characteristics of SRU in vitro fermentation based on irradiated chitosan as a feed supplement. Materials and Methods: In the first phase of the investigation, four chitosan-based SRU formulations with varying amounts of acrylamide (3 and 5 g) and gamma irradiation (5 and 10 kGy) were evaluated. Scanning electron microscopy, Fourier transform mid-infrared spectroscopy, and ammonia release characteristics were used to observe morphological, functional group, and ammonia release characteristics. In the second phase of research, the most effective SRU formulation was utilized as a supplement to ruminant rations based on rice straw, sorghum straw, and alfalfa. Gas production, rumen fermentation characteristics, and methane gas production were observed in vitro. Results: On the basis of surface image analysis, the four SRU formulas generate a similar appearance. Compared with untreated urea, the SRU3 formula reduced the percentage of ammonia emission by 12.85%-27.64% after 24 h of incubation (p = 0.05), as determined by the first phase study. SRU3 became the basis for the second testing phase. The addition of SRU3 did not affect the optimal gas production in vitro. SRU3 treatment produced less gas than Optigen® treatment (p = 0.05). With regard to rumen fermentation and digestibility, Optigen® yielded better results than SRU3 (p = 0.05). However, the treatment with SRU3 resulted in reduced methane production compared to that in the control (p = 0.05). Conclusion: Irradiated chitosan as an SRU matrix may control the release of ammonia in the rumen medium. The SRU3 formulation is the most effective. The addition of SRU to rice straw-based rations reduces methane production without affecting in vitro digestibility.

AIP conference proceedings, 2024

Penerbit BRIN eBooks, Dec 28, 2023

BIO web of conferences, Dec 31, 2022

Penelitian ini bertujuan untuk mengetahui pengaruh UMMB dan 3 pakan suplemen lainnya terhadap kua... more Penelitian ini bertujuan untuk mengetahui pengaruh UMMB dan 3 pakan suplemen lainnya terhadap kualitas cairan rumen domba. Rancangan yang digunakan adalah rancangan Bujur Sangkar Latin 4 x 4, dengan 4 macam perlakuan pakan suplemen. Parameter yang diamati : pH, amonia, daya cerna terhadap bahan kering (BK) dan bahan organik (BO), serta biomasa sel dengan P32. Data penelitian menunjukkan bahwa pengaruh perlakuan tidak berpengaruh nyata terhadap pH cairan rumen, daya cerna terhadap BK dan BO, produksi gas, sintesis mikrobia maupun efisiensi pembentukan mikrobia. Perlakuan memberikan perbedaan yang nyata terhadap kadar amonia cairan rumen. Amonia cairan rumen tertinggi dicapai pada suplemen pakan formula baru yaitu 31.39 mg/100 ml diikuti UMMB, Nutrifeed dan Nutrifeed + UMMB berturut-turut 28,58; 27,95 dan 41mg/100ml. Dari penelitian ini dapat disimpulkan bahwa suplemen pakan formula baru mempunyai peluang untuk dikembangkan.

Telah dilakukan pengujian di lapang proteksi protein dengan menggunakan tanin yang berasal dari d... more Telah dilakukan pengujian di lapang proteksi protein dengan menggunakan tanin yang berasal dari daun nangka. Ternak yang digunakan berjum]ah 20 ekor sapi Peranakan Ongole (PO) umur rata-rata 2 tahun dan rerata bobot badan awal 212 ± 18 kg. Perlakuan pakan yang digunakan adalah Pl : kontrol (rumput lapangan); P2 : kontrol positif: rumput lapangan + kedelai; P3: rumput lapangan + kedelai + tanin 0,7%; P4 rumput lapangan + kedelai + tanin 1,4%. Masing-masing perlakuan terdiri dari 8 ekor sapi sebagai ulangan. Perubahan yang diamati adalah Pertambahan Bobot Badan Harian (PBBH). Perlakuan yang paling efisien yaitu dengan pemberian rumput lapangan + kedelai + tanin 0,7% dapat meningkatkan PBBH sebesar 0,86 kg/ekor/hari lebih tinggi dibandingkan dengan kontrol positif sebesar 94,19%.

Jurnal ilmiah aplikasi isotop dan radiasi : A Scientific journal for the applications of isotipes and radiation, Dec 16, 2017

Teknik produksi gas digunakan untuk mengetahui perbandingan beberapa jenis galur mutan sorgum yan... more Teknik produksi gas digunakan untuk mengetahui perbandingan beberapa jenis galur mutan sorgum yang dihasilkan oleh BATAN sebagai pakan ruminansia. 8 jenis daun galur mutan sorgum dan rancangan acak lengkap digunakan dalam pengujian ini. Untuk melihat perbandingan antara perlakuan dilakukan uji anova dan uji lanjut BNT bila pengaruh perlakuan signifikan. Sampel ditimbang 375 mg, dimasukkan ke dalam syringe glass 100 ml ditambah 30 ml media campuran cairan rumen dengan buffer bicarbonat dan diinkubasi pada suhu 39 o C selama 24 jam. Variabel yang diukur adalah produksi gas setelah 0, 2, 4, 6, 8,19, 12, 24 dan 48 jam inkubasi, potensi produksi gas, degradabilitas bahan kering (DBK) dan organik (DBO). Hasil yang diperoleh menunjukkan produksi gas, potensi produksi gas dan degradabilitas yang dihasilkan berbeda nyata (P<0,05). Produksi gas tertinggi setelah 24 dan 48 jam adalah perlakuan H dan B yaitu 57,59 dan 71,75 ml/375 mg BK, sementara itu terendah adalah perlakuan I dan G yaitu 50,33 dan 54,92 ml/375 mg BK. Potensi produksi gas yang dihasilkan tertinggi adalah perlakuan B yaitu 85,46 ml/375 mg BK dan terendah adalah perlakuan G yaitu 56,74 ml/375 mg BK, sementara itu persentase produksi gas selama 24 jam tertinggi adalah perlakuan G yaitu 82,45% dan terendah perlakuan B yaitu 62,24%. Degradabilitas bahan kering (DBK) tertinggi setelah 24 dan 48 jam inkubasi adalah perlakuan D dan A yaitu 54,45 dan 67,31%, sementara itu terendah adalah perlakuan G yaitu 50,85 dan 58,93%. Degradabilitas bahan organik (DBO) tertinggi tertinggi setelah 24 dan 48 jam inkubasi adalah perlakuan D dan B yaitu 54,13 dan 69,13%, sementara itu terendah adalah perlakuan G yaitu 50,67 dan 58,93%. Secara umum ada dua jenis galur mutan sorgum hasil iradiasi yaitu mudah terdegradasi sebelum 24 jam dan setelah 24 jam, sehingga bisa digunakan untuk pedoman pemberian pakan untuk ruminansia.

Buletin Fakultas Peternakan Universitas Gadjah Mada, Feb 27, 2017

Advances in Animal and Veterinary Sciences, 2022

Our objective was to investigate the effect of different variety and wilting treatment on silage ... more Our objective was to investigate the effect of different variety and wilting treatment on silage quality and in vitro degradability of whole-plant sorghum. Three sorghum varieties (Numbu, Super 1 and Samurai 1) were ensiled either fresh or wilted and evaluated in a 2 x 3 factorial arrangement. Based on sensory evaluation, colour, smell and sensory index increased after wilting treatment (P < 0.01). Based on chemical quality, pH and NH 3-N values were lower in wilted groups than in unwilting sorghum silage (P < 0.01). Compared with non-wilted materials, higher dry-matter (DM) and organic-matter (OM) content were found in wilted materials (P < 0.01). Wilting did not affect crude protein (CP), ether extract (EE), neutral detergent fibre (NDF), acid detergent fibre (ADF), hemicellulose, cellulose, or non-fibre carbohydrate (NFC) content. Samurai 1 sorghum silage had the lowest NDF and ADF, both in non-wilted and wilted materials (P < 0.05). The interaction of wilting and different variety had a significant impact on NDF (P < 0.05), ADF, OM, and CP (P < 0.01). Wilting treatment had no significant impact on all aspects of in vitro degradability. In contrast, variety difference had a significant impact on in vitro degradability (P < 0.01). Results of the current study indicate that wilting treatment influences the sensory score and chemical quality of sorghum silage. There was no effect on nutrient composition or in vitro digestibility. The effect of different variety on the nutrient value of sorghum silage was more pronounced than the wilting variable.

Advances in Animal and Veterinary Sciences, 2022

This work investigates the effects of different soil pH and different genotypes on the nutrient p... more This work investigates the effects of different soil pH and different genotypes on the nutrient profiles and in vitro digestibility of soybeans. Thirty soybean genotypes were evaluated for nutrient composition, fiber content, and in vitro digestibility after cultivation in pH 4.0 and 5.4 soils. The experiment was arranged factorially in a completely randomized block design. The experimental factors were soybean genotype and soil pH. Results showed significant (p < 0.001) differences in fiber content, non-fiber carbohydrate content, and in vitro dry matter digestibility (IVDMD) parameters among studied genotypes. There were significant differences (p < 0.05) between the two soil pH conditions for ash, organic matter, ether extract, neutral detergent fiber, acid detergent fiber, hemicellulose, and nonfiber carbohydrate. Organic matter, crude protein, ether extract, and non-fiber carbohydrate content in soil with pH 5.4

IOP conference series, Nov 1, 2021

In vitro and in vivo testing for ruminant feed efficiency can be done by utilizing the stable iso... more In vitro and in vivo testing for ruminant feed efficiency can be done by utilizing the stable isotope Nitrogen-15 (15N) as a tracer. Feed can be traced by labeling the forage using isotope 15N. Feed crops are labeled using an isotope 15N-enriched fertilizer. The critical thing to note is to know the content of isotopes 15N in the part of forage feed plants that have been labeled. This research aims to know the effect of urea fertilizer on the percent of atom excess 15N on corn. Corn are labeled using urea enriched with isotopes 15N in the form of urea fertilizer (10% excess atom 15N) with different doses (0-200% recommended urea dose). As a control used corn plants given urea fertilizer is not labeled 15N. The results showed that corn forage feed was successfully labeled and correlated with the dose of fertilizer. The range of atom excess 15N was 4.28 – 6.99% in corn forage. Biomass production showed no significant difference between the dose of fertilization and control, but neither protein content. Based on data, the corn forage can be used for further testing.

Journal of animal health and production, 2021

This study investigated the effect of harvesting time on concentration and biological activity of... more This study investigated the effect of harvesting time on concentration and biological activity of tannin in Andrographis paniculata (A. paniculata) leaves and determined the in vitro rumen fermentation and digestibility of diet supplemented with A. paniculata leaves to evaluate its role as herbal supplement in ruminant's feed. A. paniculata leaves were harvested at two time points i.e., before and after flowering phase and the samples were dried to analyze concentration and biological activity of tannin. Rice straw was used as basal diet to carry out in vitro digestibility trial. Four treatments were used including B consisting basal diet only, BBF and BAF each consisting basal diet supplemented with 1% A. paniculata leaves before and after flowering phases, respectively, and BBAF supplemented with 0.5% A. paniculata leaves before and 0.5% after flowering phase. Each treatment was carried out in five replications. Results revealed that contents (mg/g) for total tannin (20.40±1.06 vs 19.33±1.19; P<0.05) and condensed tannin (8.44±0.17 vs 5.47±0.30; P<0.01) were greater in leaves harvested after flowering compared with before flowering phase, however, no significant difference (P > 0.05) on biological activity of tannins was observed between the two flowering phases. The in vitro gas production was not influenced (P > 0.05) by the supplementation of A. paniculata leaves before and after flowering phase. A. paniculata supplementation also did not influence (P > 0.05) in vitro organic matter digestibility (IVOMD) and total volatile fatty acids (TVFA) values. It was concluded that the supplementation of A. paniculata leaves before and after flowering phase in the diet does not affect the digestibility.

Advances in Animal and Veterinary Sciences, 2020

Five rice straw cultivars in Indonesia (Atomita 1, Bestari, Inpari Sidenuk, Situ Gintung and Cihe... more Five rice straw cultivars in Indonesia (Atomita 1, Bestari, Inpari Sidenuk, Situ Gintung and Ciherang) were investigated for nutrient and in vitro digestibility as ruminants roughage. Except Ciherang, all cultivars were mutant rice variety. This study aimed to: 1) assess the influence of variety on the nutrient and fiber variation of rice straw; 2) predict the nutrient value of rice straw using fiber content; and 3) evaluate the in vitro digestibility of five rice straw cultivars in Indonesia. Except for acid detergent lignin (ADL) (P= 0.09), a significant difference (P<0.05) were observed for all nutrient and fiber contents between all varieties. Acid detergent fiber (ADF) and cellulose content in mutant varieties were significantly higher than Ciherang variety. Based on fiber content, the range in relative feed value (RFV) varied by 60.99-68.89. However, all rice straw varieties are included in reject forage class. There were significant differences at 48 and 72 h in vitro gas production (P<0.05) between all varieties. Highly significant differences (P<0.001) were observed for optimum (a+b) and rate gas production (c) traits. The in vitro organic matter digestibility (IVOMD) varied from 30.30-35.87%. Those results could explain differences in nutritional quality and digestibility of rice straw according to cultivars. Ciherang variety had a good prospect for ruminant roughage due to the highest nutrient value and digestibility.

IOP conference series, Jun 1, 2019

Livestock, as part of agriculture sector, has contribute to greenhouse gas (GHG) emission especia... more Livestock, as part of agriculture sector, has contribute to greenhouse gas (GHG) emission especially on methane (CH4) compounds. Methane emission represent energy losses in form of gas reflecting low feed efficiency. Rice straw is a source commonly used as animal roughage. The quality of rice straw could be improved by fermentation treatment. This study investigated effects of fermentation using Phanerochaete chrysosporium and Trichoderma viride inoculant on in vitro gas and methane emission from rice straw. The rice straw was fermented in three weeks. This experiment consisted of four treatments and three replications being: (1) C (control, fermented rice straw with no inoculant), (2) TV (fermented rice straw using T. viride inoculant), (3) PC (fermented rice straw using P. chrysosporium inoculant), (4) TVPC (fermented rice straw using T. viride and P. chrysosporium inoculant). This research was arranged into a completely randomized block design. Results showed that TVPC treatment produced the lowest lignin content (p<0.05). TVPC treatment also produced the highest glucose compound by 1.70 mg/g (p<0.05). Meanwhile, there was no difference between all treatments in in vitro total gas and optimum gas (a+b) production. However, adding T. viride combination with P. chrysosporium in fermented process could decrease methane gas production (P<0.01). Results demonstrate that adding T. viride and P. chrysosporium inoculant in fermented process could increase the efficiency of rice straw as ruminant's feed. This was represent in the low methane production and could reduce a part of GHG emission from enteric fermentation.

Livestock and Animal Research, Nov 26, 2020

Penelitian ini bertujuan untuk mengevaluasi pengaruh iradiasi gamma terhadap kandungan nutrien, f... more Penelitian ini bertujuan untuk mengevaluasi pengaruh iradiasi gamma terhadap kandungan nutrien, fenol dan aktivitas biologis tanin daun nangka (Artocarpus heterophyllus). Aktivitas biologis yang dimaksud adalah kemampuan tanin dalam mengikat protein yang terkandung dalam substrat pakan. Metode: Perlakuan penelitian adalah: DN (daun nangka tanpa iradiasi gamma); DN 5 (daun nangka yang diiradiasi gamma pada dosis 5 kGy); DN 7,5 (daun nangka yang diiradiasi gamma pada dosis 7,5 kGy) dan DN 10 (daun nangka yang diiradiasi gamma pada dosis 10 kGy). Variabel yang diamati adalah profil nutrien, fraksi serat, anti nutrien dan aktivitas biologis tanin. Pada pengujian aktivitas biologis tanin, keempat perlakuan ditambahkan polietilen glikol (PEG) sebagai agen inaktivasi tanin. Rancangan Acak Lengkap (RAL) dengan lima ulangan digunakan dalam penelitian ini. Hasil: Hasil penelitian menunjukkan bahwa iradiasi gamma tidak memberikan pengaruh yang nyata terhadap kandungan bahan organik (BO), protein kasar (PK), acid detergent fiber (ADF), total phenol dan total tanin pada daun nangka. Iradiasi gamma justru menurunkan aktivitas biologis tanin yang direpresentasikan oleh produksi gas kumulatif in vitro. Walaupun demikian, dosis iradiasi gamma 5; 7,5 dan 10 kGy mampu menurunkan kandungan lemak kasar (LK) daun nangka berturut-turut sebesar 57,82; 83,64 dan 97,09% dari kandungan awal. Kesimpulan: Kesimpulan penelitian ini adalah Aktivitas biologis tanin pada daun nangka menurun setelah diiradiasi gamma pada dosis > 5 kGy. Dosis iradiasi gamma 7,5 kGy mampu meningkatkan produksi gas komulatif dan kecernaan bahan organik in vitro daun nangka.

Advances in Animal and Veterinary Sciences, 2021

The comparison was made between eighteen mutant and four non-mutant rice straw variety in Indones... more The comparison was made between eighteen mutant and four non-mutant rice straw variety in Indonesia in terms of chemical composition, nutrient value and in vitro digestibility. The second objective was to determine the correlation between in vitro digestibility parameters and fibrous component in twenty-two Indonesian rice straw varieties. Rice straw samples were collected in triplicate from three replicate plots. The effect of variety on rice straw quality, nutrient values and in vitro digestibility was analyzed using one-way analysis of variance (ANOVA). Results demonstrated that the rice straw varieties differed (P<0.