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Papers by Wayan Karta

Jurnal Abdimas Ilmiah Citra Bakti, Nov 21, 2022

Students need to have entrepreneurial skills, so that apart from having the ability in the field ... more Students need to have entrepreneurial skills, so that apart from having the ability in the field of science, they also have the ability to be competitive in entrepreneurship. So far, salak processed products at Agro Abian Salak have problems regarding product marketing. This service aims to enhance the entrepreneurial spirit of Poltekkes Kemenkes Denpasar alumni and students through product creation, product production, and product marketing of Sibetan salak items. Students are formed in groups and trained from the production process to marketing. The method in this activity uses the Participatory Action Learning System (PALS) approach. In the process of activities carried out online and offline. The target audience in this program is to involve 20 prospective entrepreneurs. According to the program's findings, tenants are already motivated and knowledgeable about entrepreneurship, as evidenced by their dedication to operating a business and their labeling, marketing, and sales efforts. This program has given tenants start-up funding for their businesses, enabling them to grow into young entrepreneurs. Participants have had the opportunity to create product labeling and packaging, as well as advertise it on social media, at booths, and in online shops. The three phases of the coaching process that the participants underwent were entrepreneurial awareness, capacity building and mentorship, and institutionalization and cooperation. To support MSME actors' business growth and advancement, Agro Abian Salak has received assistance in the process of promoting and selling its products.

HAYATI Journal of Biosciences

Cardiovascular disease (CVD) is the leading cause of global mortality and disability. Hyperlipide... more Cardiovascular disease (CVD) is the leading cause of global mortality and disability. Hyperlipidemia is a major risk factor for CVD that can be controlled through medical therapy, appropriate nutrition, and lifestyle. This study aimed to identify the cang salak tea diet’s effect on the ApoC3 gene expression in a hyperlipidemia rat. 18 male Wistar rats were divided equally into three groups. A high-fat-diet-induced two groups of rats, and one group was the control. Once hyperlipidemia had been achieved, one of the two groups was treated with the cang salak tea, and one group was given a standard diet for four weeks. Authenticated rat and liver tissue were collected as a source of RNA isolation. Isolated RNA was used as a reaction template for the relative quantitation qPCR using β-actin as the housekeeping gene. The ApoC3 gene was specifically amplified with a Tm value of 82.73°C, Cq 17-19, and produced a sigmoid curve. The relative expression level of the ApoC3 gene in hyperlipidemi...

JURNAL SKALA HUSADA : THE JOURNAL OF HEALTH

ABSTRACTMotorcycle drivers have a high risk of exposure from motor vehicle exhaust such as sulfur... more ABSTRACTMotorcycle drivers have a high risk of exposure from motor vehicle exhaust such as sulfur dioxide (SO2), carbon monoxide (CO) and nitrogen dioxide (NO2). Exhaust from vehicle exhaust can affect the concentration of hemoglobin especially carbon monoxide can increase the concentration of hemoglobin. In addition to motor vehicle fumes, hemoglobin levels are also affected by age, sex, smoking habits, pregnancy, malnutrition (iron, folate, vitamin B12, vitamin A), acute and chronic inflammation, parasitic infections and congenital diseases. Purposed: describe the hemoglobin of Gojek drivers at Office West Teuku Umar, Denpasar. Method: used descriptive by flow cytometry method used automatic analyzer CELL-DYN Ruby.Total respondents were 30 persons taken by accidental sampling meeting the inclusion criteria. In this study. Result: used descriptive by flow cytometry method used automatic analyzer CELL-DYN Ruby.Total respondents were 30 persons taken by accidental sampling meeting th...

Meditory : The Journal of Medical Laboratory

Background: Diets that contain lots of antioxidants will reduce the risk caused by degenerative d... more Background: Diets that contain lots of antioxidants will reduce the risk caused by degenerative diseases. Based on this, many people develop food or health products that contain antioxidants. Jempiring leaves (Gardenia jasminoides Ellis) and Secang wood (Caesalpinia sappan L.) contain bioactive and antioxidant compositions that are beneficial to health, so they are developed into herbal products.Purpose: To analyze phytochemical reserves, antioxidant capacity and conduct organoleptic tests for Ring Cang tea, describing the benefits of ring rings based on laboratory test results and literature studies on jempiring leaves and secang wood.Method: This research is a descriptive study by conducting phytochemical testing laboratory tests and DPPH antioxidant methods and conducting organoleptic tests with 15 panelists.Results: Ring tea can contain flavonoids, tannins, terpenoids, alkaloids and polyphenols. Ring Cang tea has an antioxidant capacity of 291.43 mg / L and IC50 1.53 μg / mL whi...

JST (Jurnal Sains dan Teknologi)

The bark of Sibetan snakefruit (Salacca zalacca) and sappan wood (Caesalpinia sappan L.) are comb... more The bark of Sibetan snakefruit (Salacca zalacca) and sappan wood (Caesalpinia sappan L.) are combined into the product of Cang Salak Tea (CST). This tea is expected to be useful for diseases associated with dyslipidemia. The purpose of this study was to examine the secondary metabolites in CST and to determine the effect of CST treatment on the lipid profile of total cholesterol (TC), triglyceride (TG), HDL, and LDL level. Wistar rats were divided into three groups: control group (K), hyperlipidemic rats group given CST (P1), and hyperlipidemic rat group without CST treatment. Group P1 was given CST as much as 1.2 mL per day for 4 weeks. CST contains alkaloid, flavanoid, tannin, phenol, and saponine. Flavonoid level about 81.026 ppm; tannin 525.706 ppm, IC50 177.689 ppm, and phenol 622.7844 ppm. There were significant differences in the lipid profile of K and P1 with P2. CST treatment to hyperlipidemic rats can reduce total cholesterol and normalize level of TG, HDL, and LDL. The ac...

Biomedika, Mar 25, 2021

Blondo merupakan limbah produksi virgin coconut oil (VCO) dapat menjadi sumber untuk mengisolasi ... more Blondo merupakan limbah produksi virgin coconut oil (VCO) dapat menjadi sumber untuk mengisolasi bakteri asam laktat. Bakteri asam laktat mampu menghasilkan zat antimikroba yang dapat dikembangkan untuk mengobati kasus infeksi oleh bakteri Staphylococcus aureus. Bakteri Staphylococcus aureus dapat menyebabkan infeksi mulai dari keracunan makanan, infeksi kulit hingga infeksi sistemik. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antimikroba bakteri asam laktat dari blondo terhadap bakteri Staphylococcus aureus. Penelitian ini merupakan penelitian deskriptif cross-sectional. Sampel blondo diambil dari kelompok wanita tani di Tabanan yang berjumlah 16 sampel dengan pengambilan sampel secara simple random sampling. Bakteri diisolasi menggunakan media selektif yaitu agar de Man Rogosa and Sharpe (MRS) dan identifikasi dengan uji pewarnaan gram, uji katalase dan uji produksi gas dari glukosa. Uji aktivitas antimikroba bakteri asam laktat dilakukan dengan menggunakan metode difusi sumuran. Hasil penelitian ini menunjukkan bahwa di dalam sampel blondo terdapat bakteri asam laktat dari genus Lactobacillus dan Streptococcus. Bakteri asam laktat yang diisolasi dari blondo VCO mampu menghambat Staphylococcus aureus dengan terbentuknya zona hambat berupa zona bening dengan rata-rata diameter zona hambat 11,02 mm. Zona hambat yang terbentuk termasuk dalam kategori sedang dan kuat, dengan zona hambat tertinggi yang dihasilkan yaitu 20,8 mm dan terendah yaitu 9,2 mm. Bakteri asam laktat dari blondo memiliki aktivitas antimikroba terhadap bakteri Staphylococcus aureus.

Jurnal Penelitian Pascapanen Pertanian, 2021

The dangers of resistance to Propionibacterium acnes and the high cost of treatment prompted the ... more The dangers of resistance to Propionibacterium acnes and the high cost of treatment prompted the discovery of medicinal sources from natural substances that could act as antibacterials. One of them is using post-harvest coconut products in the form of virgin coconut oil (VCO) with extract of intaran leaves (Azadirachta indica). The purpose of this study was to determine the phytochemical characteristics, inhibition zone and their categorization and to study the difference or effect of VCO supplemented with extracts of intaran leaves (Azadirachta indica) with various concentrations on the growth of Propionibacterium acnes. This study used a posttestonly control group design. The VCO mixed with extracts of intaran leaves with various concentrations of 5%, 10%, 15%, 20% and 25%. The antibacterial test was carried out using the Kirby-Bauer disc diffusion method. Data analysis used the Kruskall Wallis and Mann Whitney test. The VCO mixture supplemented with extracts of intaran leaves sho...

JST (Jurnal Sains dan Teknologi), 2021

Fatty acid content from virgin coconut oil (VCO) is a medium-chain triglyceride (MCT) group. MCT ... more Fatty acid content from virgin coconut oil (VCO) is a medium-chain triglyceride (MCT) group. MCT is stable at very low and high temperatures, and the color does not turn black due to the addition of heat so that it can be developed into beneficial cooking oil for health. Therefore, a study was conducted on the content of fatty acids in VCO after being heated at temperatures of 150, 200, and 250°C for 60 minutes. Analysis of fatty acid content in control VCO(T0), VCO with heating temperatures of 150oC(T1), 200oC(T2), and 250oC(T3) was performed with GCMS QP-2010 Ultra.The results showed that there were differences in levels and types of fatty acids in VCO by treating T0, T1, T2, and T3. At these three temperatures still produce medium-chain saturated fatty acids and trans fatty acids are not produced, so that VCO can be utilized as cooking oil that has better stability and benefits for health.

Meditory : The Journal of Medical Laboratory, 2021

Poh Lembongan mango is a typical mango from the island of Nusa Penida. During the harvest season ... more Poh Lembongan mango is a typical mango from the island of Nusa Penida. During the harvest season the fruit is abundant and not used. The use of this mango fruit needs to be done research, so that it can be used as a souvenir product by processing it into jam. The purpose of this study was to analyze the nutritional content of the mango poh lembongan jam, and to test the organoleptic to determine the acceptability of the product as a souvenir.The research method used is descriptive, namely by laboratory tests and organoleptic tests. Laboratory tests were carried out by analyzing moisture content, ash content, fat content, protein content, carbohydrate content, and vitamin C. Organoleptic tests were carried out on the assessment of the aroma, taste, viscosity and acceptability parameters of 15 panelists.The results showed that the content of this jam was 19.649% water content; ash content 0.165%; fat content 0.573%; protein content of 0.907%, carbohydrate content of 78.705%, and vitam...

Indian Journal of Public Health Research & Development, 2018

Price of salacca at harvest time in Sibetan village, Karangasem is very low, consequently researc... more Price of salacca at harvest time in Sibetan village, Karangasem is very low, consequently researcher and farmers process it into vinegar. On the other hand natural compound is necessary to be used as anti-diabetic and anticancer. This study is aimed at analyzing active content of salacca vinegar, to know its potential in healthcare such as for anti-diabetic and anti-cancer. This is a descriptive research by analyzing the active content in laboratory. The result of this research shows that salacca vinegar of Sibetan village contain high levels of acid as vinegar with total of 6.68%; total of phenols is 27.47 mg / 100ml GAE, total of tannins is 71.69 mg / 100ml TAE, flavonoid content is 26.51 mg / 100mi QE, antioxidant capacity is 70.21 mg / L GAEAC, IC 50 is 103.09 mg / ml, vitamin C is 4.6547 mg / 100g. The content of chemicals such as acetic acid, tannins, antioxidants, and flavonoids are potential as anti-diabetic and anticancer, because it serves in lowering blood sugar levels, free radical scavenger, helps in curing damaged pancreatic beta cells, keep the cells from oxidation and protect cells from cancer through five mechanisms. For further research, research in vitro and in vivo of salacca vinegar as an anti-diabetic and anticancer is needed.

International Journal of Natural Science and Engineering, 2018

Nitrite is one of important food additive that used as a preservative and curing agent for the me... more Nitrite is one of important food additive that used as a preservative and curing agent for the meat product. Since the adverse effect of nitrites to the human health has been reported, the government regulated maximum allowable nitrite content in meat product is 125 mg/kg. Hence, continuous monitoring of the existence of nitrite, especially in the meat products are important to conducted. Quantitative analysis of nitrite in the meat product, especially sausage has been described. The spectrophotometric measurement of the nitrite contents was conducted by using Griess method. The absorbance of both standard solution and sample carried after 30 minutes at the maximum wavelength 520 nm. A linier calibration curve was obtained in the range of 0.1-0.5 ppm, with the R2 value is 0.997. Subsequently, the method was applied to determine the nitrit contents in sausage samples. The spectrophotometric determination showed there were 6 samples have nitrite contents more than maximum allowable ni...

ABSTRAK Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak etanol akar tanaman... more ABSTRAK Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak etanol akar tanaman bama (Plumbago zeylanica) berbagai konsentrasi terhadap pertumbuhan jamur dermatofit Trichophyton mentagrophytes. Penelitian adalah eksperimen murni (true experiment) dengan posttest only control group design. Ekstraksi akar tanaman bama dilakukan dengan cara maserasi menggunakan pelarut etanol absolut. Untuk menguji aktivitasnya dilakukan uji daya hambat dengan metode difusi dan penentuan konsentrasi hambatan minimum (KHM) dengan metode dilusi. Analisis dilakukan secara statisik menggunakan uji one way ANOVA dengan signifikansi p = 0,05. Ekstrak akar tanaman bama (P. zeylanica) berbagai konsentrasi mampu menghambat pertumbuhan jamur dermatofit T. mentagrophytes. Berdasarkan sensitivitasnya, konsentrasi pada 1,5%; 2,5%; 5%, dan 10% dalam kategori sensitif sama dengan kontrol positif ketokonazol 2%. Uji One Way Anova menunjukkan p lebih kecil dari 0,05 yang berarti terdapat perbedaan zon...

At harvest time snake fruit “salak” becomes very cheap price, so do not give a good impact for fa... more At harvest time snake fruit “salak” becomes very cheap price, so do not give a good impact for farmers. To overcome this, the farmers have to process fruits into products such as dodol, sweets, crisps, sweets, and extract. This processing produces seeds untapped. This study aims to cultivate beans into coffee salacca and analyze its contents are potentially as food and health products such as antioxidants, caffeine, and proximate analysis. This research is a descriptive study that examines the making of coffee salacca and its contents. The process of making and sampling was conducted in Abian Salak Farmers Group, Sibetan village, Karangasem. Sample preparation done at the Department of Health Analyst Poltekkes Denpasar. The testing process sample analysis carried out in the Laboratory Services Unit of the Faculty of Agricultural Technology Udayana University. Data were analyzed descriptively by presenting tables and narrative of the results obtained to the potential of the product....

JST (Jurnal Sains dan Teknologi), 2017

Prevalensi kandidiasis meningkat seiring dengan meningkatnya populasi penderita imunokompromais. ... more Prevalensi kandidiasis meningkat seiring dengan meningkatnya populasi penderita imunokompromais. Resistensi C. albicans terhadap berbagai agen anti fungi juga dilaporkan mengalami peningkatan. Pengembangan obat yang berbasis bahan alam dan dapat bertindak sebagai anti fungi terhadap C. albicans perlu terus dilakukan. Penelitian ini bertujuan untuk mengetahui daya hambat VCO terhadap pertumbuhan C. albicans yang diisolasi dari swab vagina. Isolasi dilakukan dengan metode kultur, identifikasi mikroskopis, dan uji biokimia. Uji daya hambat VCO terhadap C. albicans isolat vagina dilakukan dengan metode difusi cakram. Jamur C. albicans berhasil diisolasi dari swab vagina dengan ciri-ciri koloni yeast, membentuk blastospora, pseudohifa, klamidopora, dan germ tube. Hasil uji difusi cakram menunjukkan VCO mampu menghambat pertumbuhan jamur C. albicans. VCO pada konsentrasi 90 % memiliki daya hambat tertinggi, dengan nilai zona hambat minimum 24,0 mm, lebih besar dibandingkan VCO pada ...

JST (Jurnal Sains dan Teknologi), 2020

Diare masih menjadi masalah global dengan derajat kesakitan dan kematian yang tinggi di berbagai ... more Diare masih menjadi masalah global dengan derajat kesakitan dan kematian yang tinggi di berbagai negara. Diare dapat menyerang wisatawan yang mengunjungi objek wisata di kawasan negara berkembang, termasuk Indonesia. Salah satu penyebab diare wisatawan adalah bakteri Escherichia coli pathogen yang memiliki gen penyandi protein transport yang berperan dalam patogenesis diare. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen penyandi protein transport sebagai kandidat vaksin subunit terhadap E. coli penyebab diare wisatawan.. Pengambilan sampel feses dilakukan di Ubud Care Clinic pada wisatawan yang melakukan pemeriksaan diare. Sampel dikultur pada media Eosin Metilen Blue, koloni tersangka kemudian diuji biokimia untuk mengkonfirmasi jenis bakteri yang ditemukan. Isolat positif E.coli selanjutnya disubkultur pada media Nutriet Broth, kemudian DNA E.coli diisolasi dengan GeneJET Genomic DNA Purification Kit. DNA hasil isolasi selanjutnya digunakan sebagai templat...

Meditory : The Journal of Medical Laboratory, 2019

This study aims to treat salacca peel and secang wood waste into herbal tea and analyze the phyt... more This study aims to treat salacca peel and secang wood waste into herbal tea and analyze the phytochemical content and antioxidant capacity and organoleptic test of the products made. Salak skin waste samples were taken at the salak center in Sibetan Village and secang wood obtained in Tenganan Village, Karangasem. Antioxidant capacity testing was carried out in the Laboratory Service Unit of the Faculty of Agricultural Technology at UNUD, and phytochemical tests at the Health Polytechnic Department of Denpasar. Organoleptic tests were carried out on 20 panelists. Tea is made by mixing secang wood powder which has been mashed with salak skin powder using 3 variations, namely VR1 (1.5 gram: 0.5 gram), VR2 (1gram: 1 gram), VR3 (0.5 gram: 1.5 gram) ), then soaked in hot water and analyzed.The results showed that Cang Salak Tea with variations of VR1, VR2, and VR3 has active phytochemical content of flavonoids, tannins, alkaloids, terpenoids, and phenols. These compounds have the potent...

Prosiding Seminar Nasional Mipa, Dec 15, 2013

Abstrak KWT BaliCocos merupakan kelompok wanita tani di Desa Tengkudak yang memiliki potensi laha... more Abstrak KWT BaliCocos merupakan kelompok wanita tani di Desa Tengkudak yang memiliki potensi lahan kelapa yang luas. Namun, tingkat perekonomiannya masih belum mengalami peningkatan dari hasil kelapanya. Hal ini karena kelapa yang dihasilkan belum diolah menjadi produk bernilai ekonomis, sehingga perlu dilakukan penelitian dan pembinaan produksi VCO. Penelitian ini bertujuan untuk menganalisis hasil produksi VCO kelompok wanita tani dan melakukan pembinaan dalam mengembangkan diversifikasi produknya, sehingga memiliki daya saing dalam pemasaran. Penelitian dilakukan melalui dua tahap yaitu (1) produksi dan analisis VCO, (2) pengembangan diversifikasi produk dan strategi pemasaran. Teknik pembuatan VCO dilakukan dengan cara basah. Hasil penelitian menunjukkan VCO yang dihasilkan memiliki bau khas kelapa segar, tidak tengik, rasa normal khas minyak kelapa, tidak berwarna, mengandung asam kaproat 0,287%; asam kaprilat 6,115%; asam kaprat 5,832%; asam laurat 48,73%; asam miristat 18,505%; asam palmitat 8,754%; asam stearat 2,606%; asam oleat 10,476%; asam linoleat 0,060%, asam linolenat 0,062%, serta tidak mengandung cemaran mikroba. Hasil ini menunjukkan kesesuaian dengan SNI 7381:2008 sehingga sangat berpotensi dikembangkan produksi dan pemasarannya. Pengembangan produk yang telah dilakukan yaitu produksi VCO original dan aromaterapi. Pemasaran dilakukan melalui toko, apotik, spa, pameran, reseller, dan jejaring media sosial. Produk VCO sangat bermanfaat untuk pangan, kesehatan/pengobatan, dan kosmetik sehingga memiliki potensi pasar yang sangat tinggi.

JST (Jurnal Sains dan Teknologi), 2017

Cara mengusir lalat daging pada sarana upacara yadnya di Bali belum efektif, sehingga diperlukan ... more Cara mengusir lalat daging pada sarana upacara yadnya di Bali belum efektif, sehingga diperlukan cara alternatif menggunakan daun mimba (Azadirachta indica). Tujuan penelitian ini untuk mendeskripsikan efektivitas larvasida daun mimba terhadap larva lalat Sarchopaga pada daging yang digunakan sebagai sarana upakara di Bali. Kelompok perlakuan ini yaitu penggunaan daun mimba 25% sebagai larvasida, kelompok kontrol positif yaitu penggunaan minuman bersoda, dan kelompok kontrol negatif yaitu tidak mendapat perlakuan. Hasil uji Kruskal Wallis diperoleh hasil nilai probabilitas p (0,000) < α (0,05) yang menunjukkan adanya perbedaan jumlah kematian larva pada perlakuan rebusan daun mimba 25 %, kontrol negatif dan kontrol positif. Perlakuan daun mimba konsentrasi 25% memberikan jumlah kematian larva yang lebih tinggi dibandingkan terhadap kontrol positif dengan perbedaan persentase sebesar 60%. Daun mimba lebih efektif sebagai larvasida jika dibandingkan dengan minuman bersoda. Pereb...

Jurnal Abdimas Ilmiah Citra Bakti, Nov 21, 2022

Students need to have entrepreneurial skills, so that apart from having the ability in the field ... more Students need to have entrepreneurial skills, so that apart from having the ability in the field of science, they also have the ability to be competitive in entrepreneurship. So far, salak processed products at Agro Abian Salak have problems regarding product marketing. This service aims to enhance the entrepreneurial spirit of Poltekkes Kemenkes Denpasar alumni and students through product creation, product production, and product marketing of Sibetan salak items. Students are formed in groups and trained from the production process to marketing. The method in this activity uses the Participatory Action Learning System (PALS) approach. In the process of activities carried out online and offline. The target audience in this program is to involve 20 prospective entrepreneurs. According to the program's findings, tenants are already motivated and knowledgeable about entrepreneurship, as evidenced by their dedication to operating a business and their labeling, marketing, and sales efforts. This program has given tenants start-up funding for their businesses, enabling them to grow into young entrepreneurs. Participants have had the opportunity to create product labeling and packaging, as well as advertise it on social media, at booths, and in online shops. The three phases of the coaching process that the participants underwent were entrepreneurial awareness, capacity building and mentorship, and institutionalization and cooperation. To support MSME actors' business growth and advancement, Agro Abian Salak has received assistance in the process of promoting and selling its products.

HAYATI Journal of Biosciences

Cardiovascular disease (CVD) is the leading cause of global mortality and disability. Hyperlipide... more Cardiovascular disease (CVD) is the leading cause of global mortality and disability. Hyperlipidemia is a major risk factor for CVD that can be controlled through medical therapy, appropriate nutrition, and lifestyle. This study aimed to identify the cang salak tea diet’s effect on the ApoC3 gene expression in a hyperlipidemia rat. 18 male Wistar rats were divided equally into three groups. A high-fat-diet-induced two groups of rats, and one group was the control. Once hyperlipidemia had been achieved, one of the two groups was treated with the cang salak tea, and one group was given a standard diet for four weeks. Authenticated rat and liver tissue were collected as a source of RNA isolation. Isolated RNA was used as a reaction template for the relative quantitation qPCR using β-actin as the housekeeping gene. The ApoC3 gene was specifically amplified with a Tm value of 82.73°C, Cq 17-19, and produced a sigmoid curve. The relative expression level of the ApoC3 gene in hyperlipidemi...

JURNAL SKALA HUSADA : THE JOURNAL OF HEALTH

ABSTRACTMotorcycle drivers have a high risk of exposure from motor vehicle exhaust such as sulfur... more ABSTRACTMotorcycle drivers have a high risk of exposure from motor vehicle exhaust such as sulfur dioxide (SO2), carbon monoxide (CO) and nitrogen dioxide (NO2). Exhaust from vehicle exhaust can affect the concentration of hemoglobin especially carbon monoxide can increase the concentration of hemoglobin. In addition to motor vehicle fumes, hemoglobin levels are also affected by age, sex, smoking habits, pregnancy, malnutrition (iron, folate, vitamin B12, vitamin A), acute and chronic inflammation, parasitic infections and congenital diseases. Purposed: describe the hemoglobin of Gojek drivers at Office West Teuku Umar, Denpasar. Method: used descriptive by flow cytometry method used automatic analyzer CELL-DYN Ruby.Total respondents were 30 persons taken by accidental sampling meeting the inclusion criteria. In this study. Result: used descriptive by flow cytometry method used automatic analyzer CELL-DYN Ruby.Total respondents were 30 persons taken by accidental sampling meeting th...

Meditory : The Journal of Medical Laboratory

Background: Diets that contain lots of antioxidants will reduce the risk caused by degenerative d... more Background: Diets that contain lots of antioxidants will reduce the risk caused by degenerative diseases. Based on this, many people develop food or health products that contain antioxidants. Jempiring leaves (Gardenia jasminoides Ellis) and Secang wood (Caesalpinia sappan L.) contain bioactive and antioxidant compositions that are beneficial to health, so they are developed into herbal products.Purpose: To analyze phytochemical reserves, antioxidant capacity and conduct organoleptic tests for Ring Cang tea, describing the benefits of ring rings based on laboratory test results and literature studies on jempiring leaves and secang wood.Method: This research is a descriptive study by conducting phytochemical testing laboratory tests and DPPH antioxidant methods and conducting organoleptic tests with 15 panelists.Results: Ring tea can contain flavonoids, tannins, terpenoids, alkaloids and polyphenols. Ring Cang tea has an antioxidant capacity of 291.43 mg / L and IC50 1.53 μg / mL whi...

JST (Jurnal Sains dan Teknologi)

The bark of Sibetan snakefruit (Salacca zalacca) and sappan wood (Caesalpinia sappan L.) are comb... more The bark of Sibetan snakefruit (Salacca zalacca) and sappan wood (Caesalpinia sappan L.) are combined into the product of Cang Salak Tea (CST). This tea is expected to be useful for diseases associated with dyslipidemia. The purpose of this study was to examine the secondary metabolites in CST and to determine the effect of CST treatment on the lipid profile of total cholesterol (TC), triglyceride (TG), HDL, and LDL level. Wistar rats were divided into three groups: control group (K), hyperlipidemic rats group given CST (P1), and hyperlipidemic rat group without CST treatment. Group P1 was given CST as much as 1.2 mL per day for 4 weeks. CST contains alkaloid, flavanoid, tannin, phenol, and saponine. Flavonoid level about 81.026 ppm; tannin 525.706 ppm, IC50 177.689 ppm, and phenol 622.7844 ppm. There were significant differences in the lipid profile of K and P1 with P2. CST treatment to hyperlipidemic rats can reduce total cholesterol and normalize level of TG, HDL, and LDL. The ac...

Biomedika, Mar 25, 2021

Blondo merupakan limbah produksi virgin coconut oil (VCO) dapat menjadi sumber untuk mengisolasi ... more Blondo merupakan limbah produksi virgin coconut oil (VCO) dapat menjadi sumber untuk mengisolasi bakteri asam laktat. Bakteri asam laktat mampu menghasilkan zat antimikroba yang dapat dikembangkan untuk mengobati kasus infeksi oleh bakteri Staphylococcus aureus. Bakteri Staphylococcus aureus dapat menyebabkan infeksi mulai dari keracunan makanan, infeksi kulit hingga infeksi sistemik. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antimikroba bakteri asam laktat dari blondo terhadap bakteri Staphylococcus aureus. Penelitian ini merupakan penelitian deskriptif cross-sectional. Sampel blondo diambil dari kelompok wanita tani di Tabanan yang berjumlah 16 sampel dengan pengambilan sampel secara simple random sampling. Bakteri diisolasi menggunakan media selektif yaitu agar de Man Rogosa and Sharpe (MRS) dan identifikasi dengan uji pewarnaan gram, uji katalase dan uji produksi gas dari glukosa. Uji aktivitas antimikroba bakteri asam laktat dilakukan dengan menggunakan metode difusi sumuran. Hasil penelitian ini menunjukkan bahwa di dalam sampel blondo terdapat bakteri asam laktat dari genus Lactobacillus dan Streptococcus. Bakteri asam laktat yang diisolasi dari blondo VCO mampu menghambat Staphylococcus aureus dengan terbentuknya zona hambat berupa zona bening dengan rata-rata diameter zona hambat 11,02 mm. Zona hambat yang terbentuk termasuk dalam kategori sedang dan kuat, dengan zona hambat tertinggi yang dihasilkan yaitu 20,8 mm dan terendah yaitu 9,2 mm. Bakteri asam laktat dari blondo memiliki aktivitas antimikroba terhadap bakteri Staphylococcus aureus.

Jurnal Penelitian Pascapanen Pertanian, 2021

The dangers of resistance to Propionibacterium acnes and the high cost of treatment prompted the ... more The dangers of resistance to Propionibacterium acnes and the high cost of treatment prompted the discovery of medicinal sources from natural substances that could act as antibacterials. One of them is using post-harvest coconut products in the form of virgin coconut oil (VCO) with extract of intaran leaves (Azadirachta indica). The purpose of this study was to determine the phytochemical characteristics, inhibition zone and their categorization and to study the difference or effect of VCO supplemented with extracts of intaran leaves (Azadirachta indica) with various concentrations on the growth of Propionibacterium acnes. This study used a posttestonly control group design. The VCO mixed with extracts of intaran leaves with various concentrations of 5%, 10%, 15%, 20% and 25%. The antibacterial test was carried out using the Kirby-Bauer disc diffusion method. Data analysis used the Kruskall Wallis and Mann Whitney test. The VCO mixture supplemented with extracts of intaran leaves sho...

JST (Jurnal Sains dan Teknologi), 2021

Fatty acid content from virgin coconut oil (VCO) is a medium-chain triglyceride (MCT) group. MCT ... more Fatty acid content from virgin coconut oil (VCO) is a medium-chain triglyceride (MCT) group. MCT is stable at very low and high temperatures, and the color does not turn black due to the addition of heat so that it can be developed into beneficial cooking oil for health. Therefore, a study was conducted on the content of fatty acids in VCO after being heated at temperatures of 150, 200, and 250°C for 60 minutes. Analysis of fatty acid content in control VCO(T0), VCO with heating temperatures of 150oC(T1), 200oC(T2), and 250oC(T3) was performed with GCMS QP-2010 Ultra.The results showed that there were differences in levels and types of fatty acids in VCO by treating T0, T1, T2, and T3. At these three temperatures still produce medium-chain saturated fatty acids and trans fatty acids are not produced, so that VCO can be utilized as cooking oil that has better stability and benefits for health.

Meditory : The Journal of Medical Laboratory, 2021

Poh Lembongan mango is a typical mango from the island of Nusa Penida. During the harvest season ... more Poh Lembongan mango is a typical mango from the island of Nusa Penida. During the harvest season the fruit is abundant and not used. The use of this mango fruit needs to be done research, so that it can be used as a souvenir product by processing it into jam. The purpose of this study was to analyze the nutritional content of the mango poh lembongan jam, and to test the organoleptic to determine the acceptability of the product as a souvenir.The research method used is descriptive, namely by laboratory tests and organoleptic tests. Laboratory tests were carried out by analyzing moisture content, ash content, fat content, protein content, carbohydrate content, and vitamin C. Organoleptic tests were carried out on the assessment of the aroma, taste, viscosity and acceptability parameters of 15 panelists.The results showed that the content of this jam was 19.649% water content; ash content 0.165%; fat content 0.573%; protein content of 0.907%, carbohydrate content of 78.705%, and vitam...

Indian Journal of Public Health Research & Development, 2018

Price of salacca at harvest time in Sibetan village, Karangasem is very low, consequently researc... more Price of salacca at harvest time in Sibetan village, Karangasem is very low, consequently researcher and farmers process it into vinegar. On the other hand natural compound is necessary to be used as anti-diabetic and anticancer. This study is aimed at analyzing active content of salacca vinegar, to know its potential in healthcare such as for anti-diabetic and anti-cancer. This is a descriptive research by analyzing the active content in laboratory. The result of this research shows that salacca vinegar of Sibetan village contain high levels of acid as vinegar with total of 6.68%; total of phenols is 27.47 mg / 100ml GAE, total of tannins is 71.69 mg / 100ml TAE, flavonoid content is 26.51 mg / 100mi QE, antioxidant capacity is 70.21 mg / L GAEAC, IC 50 is 103.09 mg / ml, vitamin C is 4.6547 mg / 100g. The content of chemicals such as acetic acid, tannins, antioxidants, and flavonoids are potential as anti-diabetic and anticancer, because it serves in lowering blood sugar levels, free radical scavenger, helps in curing damaged pancreatic beta cells, keep the cells from oxidation and protect cells from cancer through five mechanisms. For further research, research in vitro and in vivo of salacca vinegar as an anti-diabetic and anticancer is needed.

International Journal of Natural Science and Engineering, 2018

Nitrite is one of important food additive that used as a preservative and curing agent for the me... more Nitrite is one of important food additive that used as a preservative and curing agent for the meat product. Since the adverse effect of nitrites to the human health has been reported, the government regulated maximum allowable nitrite content in meat product is 125 mg/kg. Hence, continuous monitoring of the existence of nitrite, especially in the meat products are important to conducted. Quantitative analysis of nitrite in the meat product, especially sausage has been described. The spectrophotometric measurement of the nitrite contents was conducted by using Griess method. The absorbance of both standard solution and sample carried after 30 minutes at the maximum wavelength 520 nm. A linier calibration curve was obtained in the range of 0.1-0.5 ppm, with the R2 value is 0.997. Subsequently, the method was applied to determine the nitrit contents in sausage samples. The spectrophotometric determination showed there were 6 samples have nitrite contents more than maximum allowable ni...

ABSTRAK Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak etanol akar tanaman... more ABSTRAK Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak etanol akar tanaman bama (Plumbago zeylanica) berbagai konsentrasi terhadap pertumbuhan jamur dermatofit Trichophyton mentagrophytes. Penelitian adalah eksperimen murni (true experiment) dengan posttest only control group design. Ekstraksi akar tanaman bama dilakukan dengan cara maserasi menggunakan pelarut etanol absolut. Untuk menguji aktivitasnya dilakukan uji daya hambat dengan metode difusi dan penentuan konsentrasi hambatan minimum (KHM) dengan metode dilusi. Analisis dilakukan secara statisik menggunakan uji one way ANOVA dengan signifikansi p = 0,05. Ekstrak akar tanaman bama (P. zeylanica) berbagai konsentrasi mampu menghambat pertumbuhan jamur dermatofit T. mentagrophytes. Berdasarkan sensitivitasnya, konsentrasi pada 1,5%; 2,5%; 5%, dan 10% dalam kategori sensitif sama dengan kontrol positif ketokonazol 2%. Uji One Way Anova menunjukkan p lebih kecil dari 0,05 yang berarti terdapat perbedaan zon...

At harvest time snake fruit “salak” becomes very cheap price, so do not give a good impact for fa... more At harvest time snake fruit “salak” becomes very cheap price, so do not give a good impact for farmers. To overcome this, the farmers have to process fruits into products such as dodol, sweets, crisps, sweets, and extract. This processing produces seeds untapped. This study aims to cultivate beans into coffee salacca and analyze its contents are potentially as food and health products such as antioxidants, caffeine, and proximate analysis. This research is a descriptive study that examines the making of coffee salacca and its contents. The process of making and sampling was conducted in Abian Salak Farmers Group, Sibetan village, Karangasem. Sample preparation done at the Department of Health Analyst Poltekkes Denpasar. The testing process sample analysis carried out in the Laboratory Services Unit of the Faculty of Agricultural Technology Udayana University. Data were analyzed descriptively by presenting tables and narrative of the results obtained to the potential of the product....

JST (Jurnal Sains dan Teknologi), 2017

Prevalensi kandidiasis meningkat seiring dengan meningkatnya populasi penderita imunokompromais. ... more Prevalensi kandidiasis meningkat seiring dengan meningkatnya populasi penderita imunokompromais. Resistensi C. albicans terhadap berbagai agen anti fungi juga dilaporkan mengalami peningkatan. Pengembangan obat yang berbasis bahan alam dan dapat bertindak sebagai anti fungi terhadap C. albicans perlu terus dilakukan. Penelitian ini bertujuan untuk mengetahui daya hambat VCO terhadap pertumbuhan C. albicans yang diisolasi dari swab vagina. Isolasi dilakukan dengan metode kultur, identifikasi mikroskopis, dan uji biokimia. Uji daya hambat VCO terhadap C. albicans isolat vagina dilakukan dengan metode difusi cakram. Jamur C. albicans berhasil diisolasi dari swab vagina dengan ciri-ciri koloni yeast, membentuk blastospora, pseudohifa, klamidopora, dan germ tube. Hasil uji difusi cakram menunjukkan VCO mampu menghambat pertumbuhan jamur C. albicans. VCO pada konsentrasi 90 % memiliki daya hambat tertinggi, dengan nilai zona hambat minimum 24,0 mm, lebih besar dibandingkan VCO pada ...

JST (Jurnal Sains dan Teknologi), 2020

Diare masih menjadi masalah global dengan derajat kesakitan dan kematian yang tinggi di berbagai ... more Diare masih menjadi masalah global dengan derajat kesakitan dan kematian yang tinggi di berbagai negara. Diare dapat menyerang wisatawan yang mengunjungi objek wisata di kawasan negara berkembang, termasuk Indonesia. Salah satu penyebab diare wisatawan adalah bakteri Escherichia coli pathogen yang memiliki gen penyandi protein transport yang berperan dalam patogenesis diare. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen penyandi protein transport sebagai kandidat vaksin subunit terhadap E. coli penyebab diare wisatawan.. Pengambilan sampel feses dilakukan di Ubud Care Clinic pada wisatawan yang melakukan pemeriksaan diare. Sampel dikultur pada media Eosin Metilen Blue, koloni tersangka kemudian diuji biokimia untuk mengkonfirmasi jenis bakteri yang ditemukan. Isolat positif E.coli selanjutnya disubkultur pada media Nutriet Broth, kemudian DNA E.coli diisolasi dengan GeneJET Genomic DNA Purification Kit. DNA hasil isolasi selanjutnya digunakan sebagai templat...

Meditory : The Journal of Medical Laboratory, 2019

This study aims to treat salacca peel and secang wood waste into herbal tea and analyze the phyt... more This study aims to treat salacca peel and secang wood waste into herbal tea and analyze the phytochemical content and antioxidant capacity and organoleptic test of the products made. Salak skin waste samples were taken at the salak center in Sibetan Village and secang wood obtained in Tenganan Village, Karangasem. Antioxidant capacity testing was carried out in the Laboratory Service Unit of the Faculty of Agricultural Technology at UNUD, and phytochemical tests at the Health Polytechnic Department of Denpasar. Organoleptic tests were carried out on 20 panelists. Tea is made by mixing secang wood powder which has been mashed with salak skin powder using 3 variations, namely VR1 (1.5 gram: 0.5 gram), VR2 (1gram: 1 gram), VR3 (0.5 gram: 1.5 gram) ), then soaked in hot water and analyzed.The results showed that Cang Salak Tea with variations of VR1, VR2, and VR3 has active phytochemical content of flavonoids, tannins, alkaloids, terpenoids, and phenols. These compounds have the potent...

Prosiding Seminar Nasional Mipa, Dec 15, 2013

Abstrak KWT BaliCocos merupakan kelompok wanita tani di Desa Tengkudak yang memiliki potensi laha... more Abstrak KWT BaliCocos merupakan kelompok wanita tani di Desa Tengkudak yang memiliki potensi lahan kelapa yang luas. Namun, tingkat perekonomiannya masih belum mengalami peningkatan dari hasil kelapanya. Hal ini karena kelapa yang dihasilkan belum diolah menjadi produk bernilai ekonomis, sehingga perlu dilakukan penelitian dan pembinaan produksi VCO. Penelitian ini bertujuan untuk menganalisis hasil produksi VCO kelompok wanita tani dan melakukan pembinaan dalam mengembangkan diversifikasi produknya, sehingga memiliki daya saing dalam pemasaran. Penelitian dilakukan melalui dua tahap yaitu (1) produksi dan analisis VCO, (2) pengembangan diversifikasi produk dan strategi pemasaran. Teknik pembuatan VCO dilakukan dengan cara basah. Hasil penelitian menunjukkan VCO yang dihasilkan memiliki bau khas kelapa segar, tidak tengik, rasa normal khas minyak kelapa, tidak berwarna, mengandung asam kaproat 0,287%; asam kaprilat 6,115%; asam kaprat 5,832%; asam laurat 48,73%; asam miristat 18,505%; asam palmitat 8,754%; asam stearat 2,606%; asam oleat 10,476%; asam linoleat 0,060%, asam linolenat 0,062%, serta tidak mengandung cemaran mikroba. Hasil ini menunjukkan kesesuaian dengan SNI 7381:2008 sehingga sangat berpotensi dikembangkan produksi dan pemasarannya. Pengembangan produk yang telah dilakukan yaitu produksi VCO original dan aromaterapi. Pemasaran dilakukan melalui toko, apotik, spa, pameran, reseller, dan jejaring media sosial. Produk VCO sangat bermanfaat untuk pangan, kesehatan/pengobatan, dan kosmetik sehingga memiliki potensi pasar yang sangat tinggi.

JST (Jurnal Sains dan Teknologi), 2017

Cara mengusir lalat daging pada sarana upacara yadnya di Bali belum efektif, sehingga diperlukan ... more Cara mengusir lalat daging pada sarana upacara yadnya di Bali belum efektif, sehingga diperlukan cara alternatif menggunakan daun mimba (Azadirachta indica). Tujuan penelitian ini untuk mendeskripsikan efektivitas larvasida daun mimba terhadap larva lalat Sarchopaga pada daging yang digunakan sebagai sarana upakara di Bali. Kelompok perlakuan ini yaitu penggunaan daun mimba 25% sebagai larvasida, kelompok kontrol positif yaitu penggunaan minuman bersoda, dan kelompok kontrol negatif yaitu tidak mendapat perlakuan. Hasil uji Kruskal Wallis diperoleh hasil nilai probabilitas p (0,000) < α (0,05) yang menunjukkan adanya perbedaan jumlah kematian larva pada perlakuan rebusan daun mimba 25 %, kontrol negatif dan kontrol positif. Perlakuan daun mimba konsentrasi 25% memberikan jumlah kematian larva yang lebih tinggi dibandingkan terhadap kontrol positif dengan perbedaan persentase sebesar 60%. Daun mimba lebih efektif sebagai larvasida jika dibandingkan dengan minuman bersoda. Pereb...