Wagner Vendrame - Academia.edu (original) (raw)

Papers by Wagner Vendrame

Research paper thumbnail of In Vitro Growth Responses of Ornamental Bananas (Musa sp.) as Affected by Light Sources

Horticulturae, 2022

Light-emitting diodes (LEDs) have become very popular for the production of horticultural crops. ... more Light-emitting diodes (LEDs) have become very popular for the production of horticultural crops. LEDs represent an alternative lighting source to regular fluorescent (FL) bulbs, increasing the quality of plants and minimizing production costs. LEDs also provide selective light intensity and quality, suitable for commercial micropropagation. The objective of this study was to evaluate the growth and development of in vitro ornamental bananas under different light sources. Two ornamental banana varieties were selected for this study: Musa ‘Little Prince’ and Musa ‘Truly Tiny’. Light quality and intensity of three different light sources were evaluated: LED-1 (116 μmol m−2 s−1), LED-2 (90 μmol m−2 s−1), and FL (100 μmol m−2 s−1). Length and biomass of plantlets were greater under LED-1 compared to FL but not significantly different from LED-2. The fresh and dry weight of shoots and roots, number of leaves, and number and length of roots were not significantly different between treatmen...

Research paper thumbnail of Manejo da cultura

Já atuou como professora de Biologia em cursos preparatórios para Concursos. Possui experiência e... more Já atuou como professora de Biologia em cursos preparatórios para Concursos. Possui experiência e publicações nas áreas de produção e manejo pós-colheita de flores e paisagismo em revistas de grande impacto nacional e internacional.

Research paper thumbnail of Orchid Pollination Biology 1

The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution author... more The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations.

Research paper thumbnail of Universidade Federal de Viçosa

How to cite Complete issue More information about this article Journal's homepage in redalyc... more How to cite Complete issue More information about this article Journal's homepage in redalyc.org Scientific Information System

Research paper thumbnail of Innovation in Propagation and Cultivation of Ornamental Plants

Horticulturae

Ornamental plants are constantly being improved by new technologies and cultivation systems to pr... more Ornamental plants are constantly being improved by new technologies and cultivation systems to provide new, high-quality plant material for one of the most demanding markets in the horticulture sector [...]

Research paper thumbnail of ORCHID CRYOPRESERVATION Criopreservação de orquideas

Orchids are lush and highly valuable plants due to their diversity and the beauty of their flower... more Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low maintenance costs that provide greater storage time and that enable good phytosanitary conditions for the plant material for commercial use. Cryopreservation involves the conservation of biological materials at ultra-low temperatures, gener...

Research paper thumbnail of Techniques and Applications of In Vitro Orchid Seed Germination

In nature orchid seeds germinate only following infection by mycorrhizal fungi that provide the d... more In nature orchid seeds germinate only following infection by mycorrhizal fungi that provide the developing embryo with water, carbohydrates, minerals, and vitamins. Orchid seeds were first germinated at the base of wild-collected potted orchids, but germination was unreliable and seedling mortality rates were high. In vitro germination techniques, which were developed in the early 1900s, have resulted in more reliable germination and propagation of many orchid taxa. The earliest in vitro orchid seed germination techniques utilized mycorrhizal fungi found in nature to stimulate germination and seedling development. In 1922 Lewis Knudson germinated orchid seeds in vitro by sowing seeds on sterile nutrient medium amended with sucrose. This technique is known as asymbiotic seed germination since no fungal mycobiont is used to promote germination. For both symbiotic and asymbiotic orchid seed germination to be effective, many conditions must be addressed such as photoperiod, temperature,...

Research paper thumbnail of Preliminary Studies on In Vitro Propagation of Camu-Camu ( Myrciaria dubia ), an Important Medicinal Plant

Camu-camu ( Myrciaria dubia ) belongs to the Myrtaceae family and is a small tree, found on the m... more Camu-camu ( Myrciaria dubia ) belongs to the Myrtaceae family and is a small tree, found on the margins of rivers and lakes, which is native to the Amazon region. This species has great nutritional value, mainly due to the high levels of potassium and ascorbic acid (vitamin C), with values ranging from 1000–6000 mg/100 g in its pulp. In spite of its economic importance, little is known about the aspects of propagation of camu-camu, particularly in vitro multiplica­tion, which could significantly increase yields. Therefore, the objective of this study was to develop a feasible system for in vitro multiplication of camu-camu using somatic embryogenesis. Different concentrations of a cytokinin and an auxin in two different media were evaluated. Embryogenic callus was induced from leaf and stem explants on either Murashige and Skoog (MS) or Woody Plant Media (WPM) medium, supplemented with various concentra­tions of 2,4-dichorophenoxy acetic acid (2,4-D) (0, 1.0, 2.0, and 4.0 mg·L -1 ) ...

Research paper thumbnail of Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Crop Breeding and Applied Biotechnology

Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to ... more Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1 , 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.

Research paper thumbnail of Effects of plant growth regulator applications on malting barley in western Canada

Canadian Journal of Plant Science

Malting barley is important in western Canada, yet many malting cultivars do not meet malt qualit... more Malting barley is important in western Canada, yet many malting cultivars do not meet malt quality standards, in part due to lodging. Lodging can decrease barley yield and quality thereby reducing the acceptability for malting. In other countries, plant growth regulator (PGR) applications are used to mitigate lodging. Chlormequat chloride (chlormequat), trinexapac-ethyl (trinexapac), and ethephon were tested at five locations over 3 yr in western Canada for their ability to limit lodging, as well as their effects on yield, agronomic traits, and pre-malt quality characteristics. PGR applications occurred between Zadoks growth stage (GS) 30–33 for chlormequat and trinexapac and GS 37–49 for ethephon. Seeding rates of 200, 300, and 400 seeds m−2 of CDC Copeland barley were used to increase the likelihood of lodging. Increased seeding rate decreased tillers per plant, height, days to maturity, kernel protein, and kernel weight. Ethephon increased the number of tillers per plant and decr...

Research paper thumbnail of Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

Agronomy Science and Biotechnology

The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to h... more The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to human influence. Two of them, Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k., are endemic of the Brazilian Caatinga. Cryopreservation can provide a means for long-term preservation of endangered plant material. The aim of this study was to evaluate the efficiency of cryoprotectants for the cryopreservation of seeds from these two ­species. The treatments consisted of immersing seeds in different cryoprotectant solutions and vitrification solutions before storage in liquid nitrogen (NL) at -196 °C, as follows: T1- Control: no cryoprotectants; T2- PVS2 (10 min), T3- PVS2 + phloroglucinol 1% (10 min), T4- PVS2 (10 min) + Supercool 1%; T5- PVS2 + phloroglucinol 1% (10 min) + Supercool 1%. Seed germination was evaluated after cryopreservation. The experimental design was completely randomized with five treatments and five replicates per treatment. Seed moist...

Research paper thumbnail of Embriogênese somática em >//i L

Research paper thumbnail of Interaction of Light Intensity and Controlledrelease Fertilization Rate on Growth and Flowering of Two New Guinea Impatiens Cultivars

HortTechnology

New guinea impatiens (Impatiens hawkeri) (NGI) `Pure Beauty Rose' (PBR) and `Paradise Orchid&... more New guinea impatiens (Impatiens hawkeri) (NGI) `Pure Beauty Rose' (PBR) and `Paradise Orchid' (PO) were grown in full sun, 55% shade, or 73% shade and fertilized with a controlled-release fertilizer (CRF) [Nutricote Total 13-13-13 (13N-5.7P-10.8K), type 100] incorporated at rates of 2, 4, 6, 8, 12, 16, 20, 24, 28 and 32 lb/yard3 of growing media (1.2, 2.4, 3.6, 4.7, 7.1, 9.5, 11.9, 14.2, 16.6, and 19.0 kg·m-3). Plant quality rating, shoot dry weight, and flower number were measured at harvest and substrate samples were collected to analyze final substrate pH and electrical conductivity (EC). For both cultivars, light intensity and fertilization rate interactions were different for shoot dry weight and flower number, but there was no difference in plant quality rating between the light levels. Quality ratings of both PBR and PO plants increased as CRF rate increased to 12 to 16 lb/yard3 above these levels quality was not improved. Shoot dry weight of PBR plants grown in full ...

Research paper thumbnail of Effect of BAM-FX® in Developing a Management Program to Control Major Insect Pests of Tomato: Sweetpotato Whitefly (Hemiptera: Aleyrodidae), Thrips (Thysanoptera: Thripidae), and their Transmitted Viruses

Florida Entomologist

BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access t... more BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Research paper thumbnail of Silicon Fertilization Affects Growth of Hybrid Phalaenopsis Orchid Liners

HortTechnology

Experiments were conducted during two different time periods to determine if hybrid phalaenopsis ... more Experiments were conducted during two different time periods to determine if hybrid phalaenopsis orchid (Phalaenopsis spp.) liners accumulate silicon (Si) and if this element can affect liner growth. A total of 800 liners were evaluated and Si fertilization was performed by applying potassium silicate (KSiO3) as a drench with three treatments (0.5%, 1.0%, and 2.0% v/v) and a control (water, no Si fertilization). The application of KSiO3 affected overall growth of phalaenopsis orchid liners, where Si content of the plant ranged from 0.5% to 1.7%. Overall, Si applied at 0.5% and 1.0% increased fresh weight and dry weight (DW) and at 1.0% Si significantly increased DW of root, shoot, and whole plant over the control. Increases in DW ranged from 27% up to 118%. Results from the second experiment were similar. Other plant parameters evaluated such as leaf number and size, root number, and length were unaffected by Si application. Although leaves of phalaenopsis orchid liners treated with...

Research paper thumbnail of Greenhouse Production of Jatropha, a Potential Biofuel Crop

HortTechnology

The objective of this study was to evaluate greenhouse techniques for the production of jatropha ... more The objective of this study was to evaluate greenhouse techniques for the production of jatropha (Jatropha curcas). Jatropha seedlings were transplanted into 1-gal containers filled with bark mix, coir, or peat-based substrate and fertilized with 0, 4.1, 5.9, or 8.3 oz/ft3 of a 15N–4.05P–9.96K controlled-release fertilizer (CRF). Plants were watered every 2, 3, or 4 days for 80 days in the greenhouse. Jatropha plants grown in peat-based substrate had greater stem diameter and shoot dry weight (SDW) than plants grown in bark mix. For each growing substrate, plants fertilized with 8.3 and 5.9 oz/ft3 of CRF had greater SDW than plants fertilized with 4.1 and 0 oz/ft3 of CRF. Similarly, for all three substrates, plants irrigated every 2 or 3 days had greater SDW than plants irrigated every 4 days. Although jatropha has been classified as a low–nutrient and water requiring plant, the results of this study suggest that increased inputs of fertilizer and water produce larger plants. Furthe...

Research paper thumbnail of Advances in Coconut palm propagation

Revista Brasileira de Fruticultura

Coconut palm (Cocos nucifera L.) is one of the most important tropical species used by man, known... more Coconut palm (Cocos nucifera L.) is one of the most important tropical species used by man, known as “tree of life”. In recent decades, with the expansion of coconut growing areas, limitations such as the occurrence of pests, uniformity of crops, adaptation to different ecosystems and others have affected the production and longevity of coconut trees. This review describes the major advances in propagation techniques of coconut palm, conventionally, by seeds and through plant tissue culture techniques from the mid-twentieth century onwards, as well as phytosanitary aspects that should be considered to mitigate the spread of pests and diseases.

Research paper thumbnail of Historical Development of Composting Technology during the 20th Century

HortTechnology

Although composting has been practiced for thousands of years, it was not until the 20th century ... more Although composting has been practiced for thousands of years, it was not until the 20th century that controlled scientific studies were published illustrating the benefits of compost use in crop production. These studies helped to spur increased interest in composting and compost use, and gave way to the development of commercial composting facilities that supply finished compost products to horticultural producers. Increasing composting activity and compost use encouraged the formation in the late 20th century of trade organizations, such as the U.S. Composting Council and similar organizations in other countries, that support research and applications work to determine ways to improve quality control of commercial compost products.

Research paper thumbnail of Field Performance and Molecular Evaluations of Pecan Trees Regenerated from Somatic Embryogenic Cultures

Journal of the American Society for Horticultural Science

Field evaluations were conducted of pecan [Carya illinoinensis (Wangenh.) C. Koch] trees regenera... more Field evaluations were conducted of pecan [Carya illinoinensis (Wangenh.) C. Koch] trees regenerated via somatic embryogenesis to assess if the trees maintained clonal fidelity and exhibited true-to-type characteristics. Phenotypic and molecular comparisons were made of trees from two different tissue culture lines after 4 years in the field. Factors evaluated included shoot growth, leaf morphology, and susceptibility to fungal scab [Cladosporium caryigenum (Ellis & Langl.) Gottwald] and southern pecan leaf phylloxera (Phylloxera russellae Stoetzel). Genetic fidelity was examined using amplified fragment length polymorphism (AFLP) analysis. Statistically significant differences were observed between the culture lines in phenotypic leaf characteristics (i.e., specific leaf weight and leaf length-to-width ratio), number of shoots per 1-year-old branch, and in the frequency of scab lesions on leaves. No between-line differences were observed in trunk caliper, average and total shoot gr...

Research paper thumbnail of A Phenotypic and Molecular Evaluation of Field-grown Trees Derived from Somatic Embryogenic Cultures

HortScience

Somatic embryogenic protocols have been developed for pecan, Carya illinoinensis (Wangenh.) C. Ko... more Somatic embryogenic protocols have been developed for pecan, Carya illinoinensis (Wangenh.) C. Koch, in which high multiplication rates and efficient plant regeneration can be obtained. Regenerated plants were transferred into the field in 1995. Phenotypic and genetic comparisons were made of trees from two tissue culture lines to assess if plants derived from somatic embryogenic cultures maintain clonal fidelity and exhibit true-to-type characteristics. Genetic changes were examined using AFLP (Amplified Fragment Length Polymorphism) analyses. Shoot growth, leaf morphology, and the occurrence of scab (Cladosporium caryigenum) lesions and Phylloxera galls were evaluated. AFLP could readily detect differences between the tissue culture lines, which exhibited a higher number of polymorphic loci relative to comparisons within culture lines. Phenotypic characteristics differed between lines, but were generally consistent in trees within a culture line.

Research paper thumbnail of In Vitro Growth Responses of Ornamental Bananas (Musa sp.) as Affected by Light Sources

Horticulturae, 2022

Light-emitting diodes (LEDs) have become very popular for the production of horticultural crops. ... more Light-emitting diodes (LEDs) have become very popular for the production of horticultural crops. LEDs represent an alternative lighting source to regular fluorescent (FL) bulbs, increasing the quality of plants and minimizing production costs. LEDs also provide selective light intensity and quality, suitable for commercial micropropagation. The objective of this study was to evaluate the growth and development of in vitro ornamental bananas under different light sources. Two ornamental banana varieties were selected for this study: Musa ‘Little Prince’ and Musa ‘Truly Tiny’. Light quality and intensity of three different light sources were evaluated: LED-1 (116 μmol m−2 s−1), LED-2 (90 μmol m−2 s−1), and FL (100 μmol m−2 s−1). Length and biomass of plantlets were greater under LED-1 compared to FL but not significantly different from LED-2. The fresh and dry weight of shoots and roots, number of leaves, and number and length of roots were not significantly different between treatmen...

Research paper thumbnail of Manejo da cultura

Já atuou como professora de Biologia em cursos preparatórios para Concursos. Possui experiência e... more Já atuou como professora de Biologia em cursos preparatórios para Concursos. Possui experiência e publicações nas áreas de produção e manejo pós-colheita de flores e paisagismo em revistas de grande impacto nacional e internacional.

Research paper thumbnail of Orchid Pollination Biology 1

The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution author... more The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations.

Research paper thumbnail of Universidade Federal de Viçosa

How to cite Complete issue More information about this article Journal's homepage in redalyc... more How to cite Complete issue More information about this article Journal's homepage in redalyc.org Scientific Information System

Research paper thumbnail of Innovation in Propagation and Cultivation of Ornamental Plants

Horticulturae

Ornamental plants are constantly being improved by new technologies and cultivation systems to pr... more Ornamental plants are constantly being improved by new technologies and cultivation systems to provide new, high-quality plant material for one of the most demanding markets in the horticulture sector [...]

Research paper thumbnail of ORCHID CRYOPRESERVATION Criopreservação de orquideas

Orchids are lush and highly valuable plants due to their diversity and the beauty of their flower... more Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low maintenance costs that provide greater storage time and that enable good phytosanitary conditions for the plant material for commercial use. Cryopreservation involves the conservation of biological materials at ultra-low temperatures, gener...

Research paper thumbnail of Techniques and Applications of In Vitro Orchid Seed Germination

In nature orchid seeds germinate only following infection by mycorrhizal fungi that provide the d... more In nature orchid seeds germinate only following infection by mycorrhizal fungi that provide the developing embryo with water, carbohydrates, minerals, and vitamins. Orchid seeds were first germinated at the base of wild-collected potted orchids, but germination was unreliable and seedling mortality rates were high. In vitro germination techniques, which were developed in the early 1900s, have resulted in more reliable germination and propagation of many orchid taxa. The earliest in vitro orchid seed germination techniques utilized mycorrhizal fungi found in nature to stimulate germination and seedling development. In 1922 Lewis Knudson germinated orchid seeds in vitro by sowing seeds on sterile nutrient medium amended with sucrose. This technique is known as asymbiotic seed germination since no fungal mycobiont is used to promote germination. For both symbiotic and asymbiotic orchid seed germination to be effective, many conditions must be addressed such as photoperiod, temperature,...

Research paper thumbnail of Preliminary Studies on In Vitro Propagation of Camu-Camu ( Myrciaria dubia ), an Important Medicinal Plant

Camu-camu ( Myrciaria dubia ) belongs to the Myrtaceae family and is a small tree, found on the m... more Camu-camu ( Myrciaria dubia ) belongs to the Myrtaceae family and is a small tree, found on the margins of rivers and lakes, which is native to the Amazon region. This species has great nutritional value, mainly due to the high levels of potassium and ascorbic acid (vitamin C), with values ranging from 1000–6000 mg/100 g in its pulp. In spite of its economic importance, little is known about the aspects of propagation of camu-camu, particularly in vitro multiplica­tion, which could significantly increase yields. Therefore, the objective of this study was to develop a feasible system for in vitro multiplication of camu-camu using somatic embryogenesis. Different concentrations of a cytokinin and an auxin in two different media were evaluated. Embryogenic callus was induced from leaf and stem explants on either Murashige and Skoog (MS) or Woody Plant Media (WPM) medium, supplemented with various concentra­tions of 2,4-dichorophenoxy acetic acid (2,4-D) (0, 1.0, 2.0, and 4.0 mg·L -1 ) ...

Research paper thumbnail of Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Crop Breeding and Applied Biotechnology

Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to ... more Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1 , 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.

Research paper thumbnail of Effects of plant growth regulator applications on malting barley in western Canada

Canadian Journal of Plant Science

Malting barley is important in western Canada, yet many malting cultivars do not meet malt qualit... more Malting barley is important in western Canada, yet many malting cultivars do not meet malt quality standards, in part due to lodging. Lodging can decrease barley yield and quality thereby reducing the acceptability for malting. In other countries, plant growth regulator (PGR) applications are used to mitigate lodging. Chlormequat chloride (chlormequat), trinexapac-ethyl (trinexapac), and ethephon were tested at five locations over 3 yr in western Canada for their ability to limit lodging, as well as their effects on yield, agronomic traits, and pre-malt quality characteristics. PGR applications occurred between Zadoks growth stage (GS) 30–33 for chlormequat and trinexapac and GS 37–49 for ethephon. Seeding rates of 200, 300, and 400 seeds m−2 of CDC Copeland barley were used to increase the likelihood of lodging. Increased seeding rate decreased tillers per plant, height, days to maturity, kernel protein, and kernel weight. Ethephon increased the number of tillers per plant and decr...

Research paper thumbnail of Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

Agronomy Science and Biotechnology

The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to h... more The Brazilian Caatinga houses a number of cacti species, which are considered endangered due to human influence. Two of them, Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k., are endemic of the Brazilian Caatinga. Cryopreservation can provide a means for long-term preservation of endangered plant material. The aim of this study was to evaluate the efficiency of cryoprotectants for the cryopreservation of seeds from these two ­species. The treatments consisted of immersing seeds in different cryoprotectant solutions and vitrification solutions before storage in liquid nitrogen (NL) at -196 °C, as follows: T1- Control: no cryoprotectants; T2- PVS2 (10 min), T3- PVS2 + phloroglucinol 1% (10 min), T4- PVS2 (10 min) + Supercool 1%; T5- PVS2 + phloroglucinol 1% (10 min) + Supercool 1%. Seed germination was evaluated after cryopreservation. The experimental design was completely randomized with five treatments and five replicates per treatment. Seed moist...

Research paper thumbnail of Embriogênese somática em >//i L

Research paper thumbnail of Interaction of Light Intensity and Controlledrelease Fertilization Rate on Growth and Flowering of Two New Guinea Impatiens Cultivars

HortTechnology

New guinea impatiens (Impatiens hawkeri) (NGI) `Pure Beauty Rose' (PBR) and `Paradise Orchid&... more New guinea impatiens (Impatiens hawkeri) (NGI) `Pure Beauty Rose' (PBR) and `Paradise Orchid' (PO) were grown in full sun, 55% shade, or 73% shade and fertilized with a controlled-release fertilizer (CRF) [Nutricote Total 13-13-13 (13N-5.7P-10.8K), type 100] incorporated at rates of 2, 4, 6, 8, 12, 16, 20, 24, 28 and 32 lb/yard3 of growing media (1.2, 2.4, 3.6, 4.7, 7.1, 9.5, 11.9, 14.2, 16.6, and 19.0 kg·m-3). Plant quality rating, shoot dry weight, and flower number were measured at harvest and substrate samples were collected to analyze final substrate pH and electrical conductivity (EC). For both cultivars, light intensity and fertilization rate interactions were different for shoot dry weight and flower number, but there was no difference in plant quality rating between the light levels. Quality ratings of both PBR and PO plants increased as CRF rate increased to 12 to 16 lb/yard3 above these levels quality was not improved. Shoot dry weight of PBR plants grown in full ...

Research paper thumbnail of Effect of BAM-FX® in Developing a Management Program to Control Major Insect Pests of Tomato: Sweetpotato Whitefly (Hemiptera: Aleyrodidae), Thrips (Thysanoptera: Thripidae), and their Transmitted Viruses

Florida Entomologist

BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access t... more BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Research paper thumbnail of Silicon Fertilization Affects Growth of Hybrid Phalaenopsis Orchid Liners

HortTechnology

Experiments were conducted during two different time periods to determine if hybrid phalaenopsis ... more Experiments were conducted during two different time periods to determine if hybrid phalaenopsis orchid (Phalaenopsis spp.) liners accumulate silicon (Si) and if this element can affect liner growth. A total of 800 liners were evaluated and Si fertilization was performed by applying potassium silicate (KSiO3) as a drench with three treatments (0.5%, 1.0%, and 2.0% v/v) and a control (water, no Si fertilization). The application of KSiO3 affected overall growth of phalaenopsis orchid liners, where Si content of the plant ranged from 0.5% to 1.7%. Overall, Si applied at 0.5% and 1.0% increased fresh weight and dry weight (DW) and at 1.0% Si significantly increased DW of root, shoot, and whole plant over the control. Increases in DW ranged from 27% up to 118%. Results from the second experiment were similar. Other plant parameters evaluated such as leaf number and size, root number, and length were unaffected by Si application. Although leaves of phalaenopsis orchid liners treated with...

Research paper thumbnail of Greenhouse Production of Jatropha, a Potential Biofuel Crop

HortTechnology

The objective of this study was to evaluate greenhouse techniques for the production of jatropha ... more The objective of this study was to evaluate greenhouse techniques for the production of jatropha (Jatropha curcas). Jatropha seedlings were transplanted into 1-gal containers filled with bark mix, coir, or peat-based substrate and fertilized with 0, 4.1, 5.9, or 8.3 oz/ft3 of a 15N–4.05P–9.96K controlled-release fertilizer (CRF). Plants were watered every 2, 3, or 4 days for 80 days in the greenhouse. Jatropha plants grown in peat-based substrate had greater stem diameter and shoot dry weight (SDW) than plants grown in bark mix. For each growing substrate, plants fertilized with 8.3 and 5.9 oz/ft3 of CRF had greater SDW than plants fertilized with 4.1 and 0 oz/ft3 of CRF. Similarly, for all three substrates, plants irrigated every 2 or 3 days had greater SDW than plants irrigated every 4 days. Although jatropha has been classified as a low–nutrient and water requiring plant, the results of this study suggest that increased inputs of fertilizer and water produce larger plants. Furthe...

Research paper thumbnail of Advances in Coconut palm propagation

Revista Brasileira de Fruticultura

Coconut palm (Cocos nucifera L.) is one of the most important tropical species used by man, known... more Coconut palm (Cocos nucifera L.) is one of the most important tropical species used by man, known as “tree of life”. In recent decades, with the expansion of coconut growing areas, limitations such as the occurrence of pests, uniformity of crops, adaptation to different ecosystems and others have affected the production and longevity of coconut trees. This review describes the major advances in propagation techniques of coconut palm, conventionally, by seeds and through plant tissue culture techniques from the mid-twentieth century onwards, as well as phytosanitary aspects that should be considered to mitigate the spread of pests and diseases.

Research paper thumbnail of Historical Development of Composting Technology during the 20th Century

HortTechnology

Although composting has been practiced for thousands of years, it was not until the 20th century ... more Although composting has been practiced for thousands of years, it was not until the 20th century that controlled scientific studies were published illustrating the benefits of compost use in crop production. These studies helped to spur increased interest in composting and compost use, and gave way to the development of commercial composting facilities that supply finished compost products to horticultural producers. Increasing composting activity and compost use encouraged the formation in the late 20th century of trade organizations, such as the U.S. Composting Council and similar organizations in other countries, that support research and applications work to determine ways to improve quality control of commercial compost products.

Research paper thumbnail of Field Performance and Molecular Evaluations of Pecan Trees Regenerated from Somatic Embryogenic Cultures

Journal of the American Society for Horticultural Science

Field evaluations were conducted of pecan [Carya illinoinensis (Wangenh.) C. Koch] trees regenera... more Field evaluations were conducted of pecan [Carya illinoinensis (Wangenh.) C. Koch] trees regenerated via somatic embryogenesis to assess if the trees maintained clonal fidelity and exhibited true-to-type characteristics. Phenotypic and molecular comparisons were made of trees from two different tissue culture lines after 4 years in the field. Factors evaluated included shoot growth, leaf morphology, and susceptibility to fungal scab [Cladosporium caryigenum (Ellis & Langl.) Gottwald] and southern pecan leaf phylloxera (Phylloxera russellae Stoetzel). Genetic fidelity was examined using amplified fragment length polymorphism (AFLP) analysis. Statistically significant differences were observed between the culture lines in phenotypic leaf characteristics (i.e., specific leaf weight and leaf length-to-width ratio), number of shoots per 1-year-old branch, and in the frequency of scab lesions on leaves. No between-line differences were observed in trunk caliper, average and total shoot gr...

Research paper thumbnail of A Phenotypic and Molecular Evaluation of Field-grown Trees Derived from Somatic Embryogenic Cultures

HortScience

Somatic embryogenic protocols have been developed for pecan, Carya illinoinensis (Wangenh.) C. Ko... more Somatic embryogenic protocols have been developed for pecan, Carya illinoinensis (Wangenh.) C. Koch, in which high multiplication rates and efficient plant regeneration can be obtained. Regenerated plants were transferred into the field in 1995. Phenotypic and genetic comparisons were made of trees from two tissue culture lines to assess if plants derived from somatic embryogenic cultures maintain clonal fidelity and exhibit true-to-type characteristics. Genetic changes were examined using AFLP (Amplified Fragment Length Polymorphism) analyses. Shoot growth, leaf morphology, and the occurrence of scab (Cladosporium caryigenum) lesions and Phylloxera galls were evaluated. AFLP could readily detect differences between the tissue culture lines, which exhibited a higher number of polymorphic loci relative to comparisons within culture lines. Phenotypic characteristics differed between lines, but were generally consistent in trees within a culture line.