Renee Wagner - Academia.edu (original) (raw)

Papers by Renee Wagner

Research paper thumbnail of Interaction of two Bacillus thuringiensis δ-endotoxins with the digestive system of Lygus hesperus

Current Microbiology, 2004

Research paper thumbnail of Immunocytochemical localization of testis ecdysiotropin in the pupa of the gypsy moth, Lymantria dispar (L.) (Lepidoptera: Lymantriidae)

Research paper thumbnail of The identification of two myoinhibitory peptides, with sequence similarities to the galanins, isolated from the ventral nerve cord of Manduca sexta

Regulatory Peptides, 1995

Two new myoinhibitory peptides, Mas-MIP I and Mas-MIP II, were identified from the ventral nerve ... more Two new myoinhibitory peptides, Mas-MIP I and Mas-MIP II, were identified from the ventral nerve cord of the adult tobacco hornworm, Manduca sexta. Sequences obtained by a combination of automated Edman degradation and electrospray mass spectrometry were, respectively, AWQDLNSAW and GWQDLNSAW. The native peptides were found to co-elute with synthetic C-terminal amides on a reverse phase HPLC system. When applied to isolated ilea (anterior hindgut) of adult M. sexta, both peptides were found to significantly reduce the rate of peristalsis, or abolish peristalsis entirely, at concentrations of 1 x 10(-9) M. Both peptides share sequence similarities with Lom-MIP, a previously identified myoinhibitory peptide from Locusta migratoria, and with the N-terminal portion of vertebrate peptides in the galanin family.

Research paper thumbnail of Nutritional Composition of Potato Foliage

Journal of Agricultural and Food Chemistry, 1995

Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliag... more Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliage John M. Domek, William W. Cantelo," Renee M. Wagner, Betty W. Li, and Nancy J. Miller-Ihli Building 470, BARC-East, 10300 ...

Research paper thumbnail of In vitro metabolism of an insect neuropeptide by neural membrane preparations from Lymantria dispar

Research paper thumbnail of Immunocytochemical localization of testis ecdysiotropin in the pupa of the gypsy moth,Lymantria dispar (L.) (Lepidoptera: Lymantriidae)

Journal of Molecular Neuroscience, 1997

Research paper thumbnail of Analysis of peptides containing oxidized methionine and/or tryptophan by fast atom bombardment mass spectrometry

Biological Mass Spectrometry, 1987

Research paper thumbnail of The isolation and identification of three diuretic kinins from the abdominal ventral nerve cord of adult Helicoverpa zea

Journal of Insect Physiology, 1995

Research paper thumbnail of Characterization of digestive proteolytic activity in Lygus hesperus Knight (Hemiptera: Miridae)

Journal of Insect Physiology, 2006

The tarnished plant bug, Lygus hesperus Knight, is a pest that causes considerable economic losse... more The tarnished plant bug, Lygus hesperus Knight, is a pest that causes considerable economic losses to vegetables, cotton, canola, and alfalfa. Detailed knowledge of its digestive physiology will provide new opportunities for a sustainable pest management approach to control this insect. Little is known about the different protease class contributions to the overall digestion of a specific protein. To this end, the proteolytic activities in female adult L. hesperus salivary gland and midgut homogenates were quantified over a range of pH's and time points, and the contribution of different classes of proteases to the degradation of FITC-casein was determined. In the salivary gland, serine proteases were the predominant class responsible for caseinolytic activity, with the rate of activity increasing with increasing pH. In contrast, both aspartic and serine proteases contributed to caseinolytic activity in the midgut. Aspartic protease activity predominated at pH 5.0 and occurred immediately after incubation, whereas serine protease activity predominated at pH 7.5 after a 9h delay and was resistant to aprotinin. The salivary serine proteases were distinctly different from midgut serine proteases, based on the tissue-specific differential susceptibility to aprotinin and differing pH optima. Collectively, the caseinolytic activities complement one another, expanding the location and pH range over which digestion can occur.

Research paper thumbnail of Nutritional Composition of Potato Foliage

Journal of Agricultural and Food Chemistry, 1995

Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliag... more Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliage John M. Domek, William W. Cantelo," Renee M. Wagner, Betty W. Li, and Nancy J. Miller-Ihli Building 470, BARC-East, 10300 ...

Research paper thumbnail of Action cascade of an insect gonadotropin, testis ecdysiotropin, in male Lepidoptera

Invertebrate Reproduction & Development, 1996

Research paper thumbnail of Partial Morphological and Functional Characterization of the Corpus Allatum - Corpus Cardiacum Complex from the Two-Spotted Stinkbug, Perillus bioculatus (Hemiptera: Pentatomidae)

In Vitro Cellular and Developmental Biology--Animal, 2005

Research paper thumbnail of Interaction of Two Bacillus thuringiensis d-Endotoxins with the Digestive System of Lygus hesperus

Current Microbiology, 2004

Research paper thumbnail of Growth and development of the knapweed root weevil, Cyphocleonus achates, on a meridic larval diet

Research paper thumbnail of Isolation and identification of a new diuretic peptide from the tobacco hornworm, Manduca sexta

Biochemical and Biophysical Research Communications, 1991

Research paper thumbnail of Isolation and primary structure of a peptide from the corpora cardiaca of Heliothis zea with adipokinetic activity

Biochemical and Biophysical Research Communications, 1986

An adipokinetic hormone was isolated from the corpora cardiaca of the corn ear worm moth, Helioth... more An adipokinetic hormone was isolated from the corpora cardiaca of the corn ear worm moth, Heliothis zea, and purified by reversed phase high performance liquid chromatography. The primary structure, pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly-NH2, was determined by automated gas-phase Edman degradation of the peptide deblocked with pyroglutamic aminopeptidase, and by fast atom bombardment mass spectrometry. The hormone was synthesized and the natural and synthetic material had identical chromatographic, spectroscopic, and biological properties. The peptide was found to have lipid mobilizing activity in H. zea adults.

Research paper thumbnail of Action of Taurine, 3-aminopropanesulfonic acid, and GABA on the hindgut and heart of the cockroach,Leucophaea maderae

Archives of Insect Biochemistry and Physiology, 1991

Taurine, glycine, glutamate, and gamma-aminobutyric acid (GABA) were all present in concentration... more Taurine, glycine, glutamate, and gamma-aminobutyric acid (GABA) were all present in concentrations of greater than 1% of the total free amino acid content in the brain, thoracic, and abdominal ganglia of Leucophaea maderae. Hemolymph, subesophageal ganglia, and hindgut had substantial amounts of glutamate and glycine, but less than 0.3% taurine or GABA. Taurine, 3-aminopropanesulfonic acid (3-APS), cysteine-sulfinic acid (CSA), and GABA each had myotropic activity on the isolated cockroach hindgut, with 3-APS having the most consistent effect (ED50 = 0.63 mM), while taurine and CSA activities were similar to that of GABA on the hindgut. Both taurine and 3-APS had anti-arrhythmic effects on semi-isolated heart preparations of L. maderae, while GABA was inhibitory and induced arrhythmia. Bicuculline was antagonistic to the effects of GABA, taurine, and 3-APS on the hindgut, and induced arrhythmia in heart preparations; this arrhythmia was reversible by taurine, but not by GABA or 3-APS.

Research paper thumbnail of Structure-function analysis ofLymantria testis ecdysiotropin: A search for the active core

Archives of Insect Biochemistry and Physiology, 1998

Research paper thumbnail of Naturally occurring analogs of Lymantria testis ecdysiotropin, a gonadotropin isolated from brains of Lymantria dispar pupae1

Archives of Insect Biochemistry and Physiology, 1997

Research paper thumbnail of Testis ecdysiotropin, an insect gonadotropin that induces synthesis of ecdysteroid

Archives of Insect Biochemistry and Physiology, 2001

Testes of lepidoptera synthesized ecdysteroid in a somewhat different temporal pattern than the p... more Testes of lepidoptera synthesized ecdysteroid in a somewhat different temporal pattern than the prothoracic glands that release ecdysteroid to the hemolymph. Brain extracts from Heliothis virescens and Lymantria dispar induced testes to synthesize ecdysteroid, but did not affect prothoracic glands. The testis ecdysiotropin (LTE) was isolated from L. dispar pupal brains by a series of high-pressure chromatography steps. Its sequence was Ile-Ser-Asp-Phe-Asp-Glu-Tyr-Glu-Pro-Leu-Asn-Asp-Ala-Asp-Asn-Asn-Glu-Val-Leu-Asp-Phe-OH, of molecular mass 2,473 Daltons. The predominant signaling pathway for LTE was via G(i) protein, IP3, diacylglycerol and PKC; a modulating pathway, apparently mediated by an angiotensin II-like peptide, was controlled via G(s) protein, cAMP, and PKA. Testis ecdysteroid caused isolated testis sheaths to also synthesize a growth factor that induced development of the male genital tract. The growth factor appeared to be a glycoprotein similar to vertebrate alpha-1-glycoprotein. A polyclonal antibody to LTE indicated LTE-like peptide in L. dispar brain medial neurosecretory cells, the suboesophageal, and other ganglia, and also in its target organ, the testis sheath. LTE immunoreactivity was also seen in testis sheaths of Rhodnius prolixus. LTE-like immunoactivity was also detected in developing optic lobes, antennae, frontal ganglia, and elongating spermatids of developing L. dispar pupae. This may indicate that LTE has a role in development as well as stimulation of testis ecdysteroid synthesis. Published 2001 Wiley-Liss, Inc.

Research paper thumbnail of Interaction of two Bacillus thuringiensis δ-endotoxins with the digestive system of Lygus hesperus

Current Microbiology, 2004

Research paper thumbnail of Immunocytochemical localization of testis ecdysiotropin in the pupa of the gypsy moth, Lymantria dispar (L.) (Lepidoptera: Lymantriidae)

Research paper thumbnail of The identification of two myoinhibitory peptides, with sequence similarities to the galanins, isolated from the ventral nerve cord of Manduca sexta

Regulatory Peptides, 1995

Two new myoinhibitory peptides, Mas-MIP I and Mas-MIP II, were identified from the ventral nerve ... more Two new myoinhibitory peptides, Mas-MIP I and Mas-MIP II, were identified from the ventral nerve cord of the adult tobacco hornworm, Manduca sexta. Sequences obtained by a combination of automated Edman degradation and electrospray mass spectrometry were, respectively, AWQDLNSAW and GWQDLNSAW. The native peptides were found to co-elute with synthetic C-terminal amides on a reverse phase HPLC system. When applied to isolated ilea (anterior hindgut) of adult M. sexta, both peptides were found to significantly reduce the rate of peristalsis, or abolish peristalsis entirely, at concentrations of 1 x 10(-9) M. Both peptides share sequence similarities with Lom-MIP, a previously identified myoinhibitory peptide from Locusta migratoria, and with the N-terminal portion of vertebrate peptides in the galanin family.

Research paper thumbnail of Nutritional Composition of Potato Foliage

Journal of Agricultural and Food Chemistry, 1995

Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliag... more Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliage John M. Domek, William W. Cantelo," Renee M. Wagner, Betty W. Li, and Nancy J. Miller-Ihli Building 470, BARC-East, 10300 ...

Research paper thumbnail of In vitro metabolism of an insect neuropeptide by neural membrane preparations from Lymantria dispar

Research paper thumbnail of Immunocytochemical localization of testis ecdysiotropin in the pupa of the gypsy moth,Lymantria dispar (L.) (Lepidoptera: Lymantriidae)

Journal of Molecular Neuroscience, 1997

Research paper thumbnail of Analysis of peptides containing oxidized methionine and/or tryptophan by fast atom bombardment mass spectrometry

Biological Mass Spectrometry, 1987

Research paper thumbnail of The isolation and identification of three diuretic kinins from the abdominal ventral nerve cord of adult Helicoverpa zea

Journal of Insect Physiology, 1995

Research paper thumbnail of Characterization of digestive proteolytic activity in Lygus hesperus Knight (Hemiptera: Miridae)

Journal of Insect Physiology, 2006

The tarnished plant bug, Lygus hesperus Knight, is a pest that causes considerable economic losse... more The tarnished plant bug, Lygus hesperus Knight, is a pest that causes considerable economic losses to vegetables, cotton, canola, and alfalfa. Detailed knowledge of its digestive physiology will provide new opportunities for a sustainable pest management approach to control this insect. Little is known about the different protease class contributions to the overall digestion of a specific protein. To this end, the proteolytic activities in female adult L. hesperus salivary gland and midgut homogenates were quantified over a range of pH's and time points, and the contribution of different classes of proteases to the degradation of FITC-casein was determined. In the salivary gland, serine proteases were the predominant class responsible for caseinolytic activity, with the rate of activity increasing with increasing pH. In contrast, both aspartic and serine proteases contributed to caseinolytic activity in the midgut. Aspartic protease activity predominated at pH 5.0 and occurred immediately after incubation, whereas serine protease activity predominated at pH 7.5 after a 9h delay and was resistant to aprotinin. The salivary serine proteases were distinctly different from midgut serine proteases, based on the tissue-specific differential susceptibility to aprotinin and differing pH optima. Collectively, the caseinolytic activities complement one another, expanding the location and pH range over which digestion can occur.

Research paper thumbnail of Nutritional Composition of Potato Foliage

Journal of Agricultural and Food Chemistry, 1995

Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliag... more Page 1. 1512 J. Agric. Food Chem. 1995, 43, 151 2-1 51 5 Nutritional Composition of Potato Foliage John M. Domek, William W. Cantelo," Renee M. Wagner, Betty W. Li, and Nancy J. Miller-Ihli Building 470, BARC-East, 10300 ...

Research paper thumbnail of Action cascade of an insect gonadotropin, testis ecdysiotropin, in male Lepidoptera

Invertebrate Reproduction & Development, 1996

Research paper thumbnail of Partial Morphological and Functional Characterization of the Corpus Allatum - Corpus Cardiacum Complex from the Two-Spotted Stinkbug, Perillus bioculatus (Hemiptera: Pentatomidae)

In Vitro Cellular and Developmental Biology--Animal, 2005

Research paper thumbnail of Interaction of Two Bacillus thuringiensis d-Endotoxins with the Digestive System of Lygus hesperus

Current Microbiology, 2004

Research paper thumbnail of Growth and development of the knapweed root weevil, Cyphocleonus achates, on a meridic larval diet

Research paper thumbnail of Isolation and identification of a new diuretic peptide from the tobacco hornworm, Manduca sexta

Biochemical and Biophysical Research Communications, 1991

Research paper thumbnail of Isolation and primary structure of a peptide from the corpora cardiaca of Heliothis zea with adipokinetic activity

Biochemical and Biophysical Research Communications, 1986

An adipokinetic hormone was isolated from the corpora cardiaca of the corn ear worm moth, Helioth... more An adipokinetic hormone was isolated from the corpora cardiaca of the corn ear worm moth, Heliothis zea, and purified by reversed phase high performance liquid chromatography. The primary structure, pGlu-Leu-Thr-Phe-Thr-Ser-Ser-Trp-Gly-NH2, was determined by automated gas-phase Edman degradation of the peptide deblocked with pyroglutamic aminopeptidase, and by fast atom bombardment mass spectrometry. The hormone was synthesized and the natural and synthetic material had identical chromatographic, spectroscopic, and biological properties. The peptide was found to have lipid mobilizing activity in H. zea adults.

Research paper thumbnail of Action of Taurine, 3-aminopropanesulfonic acid, and GABA on the hindgut and heart of the cockroach,Leucophaea maderae

Archives of Insect Biochemistry and Physiology, 1991

Taurine, glycine, glutamate, and gamma-aminobutyric acid (GABA) were all present in concentration... more Taurine, glycine, glutamate, and gamma-aminobutyric acid (GABA) were all present in concentrations of greater than 1% of the total free amino acid content in the brain, thoracic, and abdominal ganglia of Leucophaea maderae. Hemolymph, subesophageal ganglia, and hindgut had substantial amounts of glutamate and glycine, but less than 0.3% taurine or GABA. Taurine, 3-aminopropanesulfonic acid (3-APS), cysteine-sulfinic acid (CSA), and GABA each had myotropic activity on the isolated cockroach hindgut, with 3-APS having the most consistent effect (ED50 = 0.63 mM), while taurine and CSA activities were similar to that of GABA on the hindgut. Both taurine and 3-APS had anti-arrhythmic effects on semi-isolated heart preparations of L. maderae, while GABA was inhibitory and induced arrhythmia. Bicuculline was antagonistic to the effects of GABA, taurine, and 3-APS on the hindgut, and induced arrhythmia in heart preparations; this arrhythmia was reversible by taurine, but not by GABA or 3-APS.

Research paper thumbnail of Structure-function analysis ofLymantria testis ecdysiotropin: A search for the active core

Archives of Insect Biochemistry and Physiology, 1998

Research paper thumbnail of Naturally occurring analogs of Lymantria testis ecdysiotropin, a gonadotropin isolated from brains of Lymantria dispar pupae1

Archives of Insect Biochemistry and Physiology, 1997

Research paper thumbnail of Testis ecdysiotropin, an insect gonadotropin that induces synthesis of ecdysteroid

Archives of Insect Biochemistry and Physiology, 2001

Testes of lepidoptera synthesized ecdysteroid in a somewhat different temporal pattern than the p... more Testes of lepidoptera synthesized ecdysteroid in a somewhat different temporal pattern than the prothoracic glands that release ecdysteroid to the hemolymph. Brain extracts from Heliothis virescens and Lymantria dispar induced testes to synthesize ecdysteroid, but did not affect prothoracic glands. The testis ecdysiotropin (LTE) was isolated from L. dispar pupal brains by a series of high-pressure chromatography steps. Its sequence was Ile-Ser-Asp-Phe-Asp-Glu-Tyr-Glu-Pro-Leu-Asn-Asp-Ala-Asp-Asn-Asn-Glu-Val-Leu-Asp-Phe-OH, of molecular mass 2,473 Daltons. The predominant signaling pathway for LTE was via G(i) protein, IP3, diacylglycerol and PKC; a modulating pathway, apparently mediated by an angiotensin II-like peptide, was controlled via G(s) protein, cAMP, and PKA. Testis ecdysteroid caused isolated testis sheaths to also synthesize a growth factor that induced development of the male genital tract. The growth factor appeared to be a glycoprotein similar to vertebrate alpha-1-glycoprotein. A polyclonal antibody to LTE indicated LTE-like peptide in L. dispar brain medial neurosecretory cells, the suboesophageal, and other ganglia, and also in its target organ, the testis sheath. LTE immunoreactivity was also seen in testis sheaths of Rhodnius prolixus. LTE-like immunoactivity was also detected in developing optic lobes, antennae, frontal ganglia, and elongating spermatids of developing L. dispar pupae. This may indicate that LTE has a role in development as well as stimulation of testis ecdysteroid synthesis. Published 2001 Wiley-Liss, Inc.