Wajad Nazeer - Academia.edu (original) (raw)

Papers by Wajad Nazeer

Pure and Applied Biology (PAB), Nov 7, 2020

Sustainable Agriculture in the Era of the OMICs Revolution

Pakistan Journal of Biochemistry and Biotechnology

Eight transgenic (Cry1Ac -endotoxin) genotypes and one non-GMO cotton (Gossypium hirsutum L.) var... more Eight transgenic (Cry1Ac -endotoxin) genotypes and one non-GMO cotton (Gossypium hirsutum L.) variety were used in the investigation. Cry1Ac protein was expressed at various extents in several strains. In a greenhouse environment, sixty days after planting, the cry 1Ac gene's expression peaked. At 60 DAP, the variety Bt.CIM-598 (1.871 g/g) expressed the most toxin of any genotype. Throughout the cropping season, larvae of the Helicoverpa armigera were gathered from every section of the nation that produces cotton. H. armigera larvae that were captured in the field were raised on artificial feeds. There was a negative relationship between the DAP and the amount of Cry1Ac protein (-0.332). The greatest amount of death was seen when first-instar larvae consumed the leaves of 60 DAP plants. It was found that transgenic variants had a considerable effect on the percentage mortality of H. armigera. MNH-886 larvae showed the highest mortality percentage when fed on leaves that were har...

Bu calisma, iyi kaliteli ve yuksek verimli pamuk cesitlerinin degerlendirmesi amaciyla yurutulmus... more Bu calisma, iyi kaliteli ve yuksek verimli pamuk cesitlerinin degerlendirmesi amaciyla yurutulmustur. Bu amacla, pamuk cesitlerindeki verim ve kalite ile ilgili farkli bitki karakterleri icin kombinasyon yetenegi etkileri hesaplanmistir. Mevcut calisma islah programlari icin farkli cesitlerin potansiyelini ortaya koymustur. FH-945, MNH-93, MNH-129, CIM-496, CIM-446 ve NIAB-78 gibi 6 ebeveyn koza agirligi ve lif mukavemeti haric bitkilerin genel ve ozel kombine yetenekleri icin farkli karakterler gostermistir. CIM-496 bitki basina koza sayisi, pamuk cigidi verimi, tiftik orani, lif uzunlugu ve lif inceligi yonunden en iyi genel kombinasyonu vermis, bunu MNH-129 hatti takip etmistir. CIM-496xNIAB-78 hibridi bitki basina koza sayisi yonunden, CIM-496xNIAB-78 hibridi pamuk cigidi verimi, MNH-129xCIM-446 hibridi en iyi tiftik orani, CIM-496xCIM-446 hibridi ise en iyi lif uzunlugu spesifik kombine yetenegini vermistir. Bazi hibritlerde ebeveynlerden birisi iyi genel kombine yetenegi goste...

European Journal of Biotechnology and Bioscience, 2019

Bangladesh Journal of Botany, Mar 31, 2022

The morpho-physiological selection and linkage between traits have been investigated. There were ... more The morpho-physiological selection and linkage between traits have been investigated. There were 20 cotton genotypes grown using RCBD with two replications at experimental area. ANOVA showed significant variation for all the traits. The GCV and PCV values of heritability and genetic advance were higher for the traits like bolls per plant, sympodial branches per plant and plant height, respectively. These traits were more under control of the genetic factors and additive gene action. There was high significant correlation between boll weight and seed cotton yield; transpiration rate and stomatal conductance. There would be yield breakthrough on considering the higher number of bolls per plant, high boll weight per plant and optimum net photosynthesis per plant as the basis of selection.

Gossypium arboreum, a cultivated cotton species (2n = 26, AA) native to Asia, possesses invaluabl... more Gossypium arboreum, a cultivated cotton species (2n = 26, AA) native to Asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. However, it is quite diffi-cult to transfer favorable traits into Upland cotton through conventional methods due to the cross-incompatibility ofG. hirsutum (2n = 52, AADD) andG. arboreum. Here, we improved an embryo rescue technique to overcome the cross-incompatibility between these two pa-rents for transferring favorable genes fromG. arboreum intoG. hirsutum. Our results indi-cate that MSB2K supplemented with 0.5 mgl-1 kinetin and 250 mg-1 casein hydrolysate is an efficient initial medium for rescuing early (3 d after pollination) hybrid embryos. Eight pu-tative hybrids were successfully obtained, which were further verified and characterized by cytology, molecular markers and morphological analysis. The putative hybrids were subse-que...

Polymer journal, 2021

Institute of Chemistry of Macromolecular Chemistry NAS of Ukraine, 48, Kharkivske shose, Kyiv, 02... more Institute of Chemistry of Macromolecular Chemistry NAS of Ukraine, 48, Kharkivske shose, Kyiv, 02160, Ukraine Developed polymer composite material with Cefazolin based on polyurethane with isocyanurate fragments (PU), synthesized on the basis of polyoxypropylene glycol (POPG 1000), TDI (2,4;2,6-toluene diisocyanate) and 2,4,6-triisocyanate(trishexamethylene) isocyanate (ICC, Tolonate ™ HDT-LV, MM 1200) at a ratio of NCO: OH = 1: 1 in the medium N,N’-dimethylacetamide (DMAA) and Cefazolin (CFZ). CFZ is immobilized on isocyanurate-containing polyurethane by introducing a solution of CFZ with DMA into the reaction the amount of 5 wt. %. The synthesized polymeric material (PU-CFZ) represents a transparent film of yellow color with physical and mechanical characteristics: σ = 0,15 MPa; ε = 63.40%. According to IR spectroscopy, Cefazolin is immobilized on the polymer matrix by physically due to hydrogen interactions. The influence of biological medium 199 (BM 199) on the structure and pro...

Agronomy, 2022

Cotton is an important agro-industrial crop across the globe. Improving the fiber quality and yie... more Cotton is an important agro-industrial crop across the globe. Improving the fiber quality and yield potential of cotton are major commercial targets for cotton breeders. The cotton lint yield is computed by multiplying three fundamental yield constituents: average boll weight, boll number per unit ground area, and lint percentage. The cotton species Gossypium arboreum exhibits a wide range of desirable traits, which are absent in the congener Gossypium hirsutum. Four parental lines of G. hirsutum and G. arboreum, with significant differences in boll-related traits, were used to develop the following four F2 populations: Mei Zhongmian × Chimu Heizi (MC), Mei Zhongmian × L-02292-3 (ML), Dixie king × Suyuan 04-44 (DS), and Dixie king × Pamuk (DP), in order to study complex traits, such as boll weight (BW) (g), lint percentage (LP) (%), boll upper width (BUW), boll medium width (BMW), boll lower width (BLU), and boll length (BL) (mm). In segregation populations, extensive phenotypic dif...

Journal of Animal and Plant Sciences, 2016

Combining ability and heterosis for within-boll yield components and fibre quality traits were ca... more Combining ability and heterosis for within-boll yield components and fibre quality traits were carried out using line × tester mating system in upland cotton at the University of Agriculture, Faisalabad, Pakistan.Variance due to specific combining ability was greater than general combining ability for all the traits depicting the importance of non-additive genetic effects.The line BH-89 was found to be good general combiner for lint mass per boll, seed volume per 100 seeds, surface area per seed and lint percentage. NIAB-999 was good general combiner for fibre fineness. The third line which was actually the F1 of the first two lines performed better regardingseed number per boll, seed mass per bolland fibre strength.Among testers, NIAB-78 exhibited higher GCA effects for seed number per boll, lint mass per boll and fibre fineness while CIM-1100 proved to be good general combiner for seed volume per 100-seeds, surface area per seed and lint percentage. NIAB-999 × VH-144 exhibited the...

Frontiers of Agriculture in China, 2011

... Pakistan Yasir Ahmed KHAN1, Wajad NAZEER (✉)2, Asifa HAMEED2, Jehanzeb FAROOQ3, Muhammad Rafi... more ... Pakistan Yasir Ahmed KHAN1, Wajad NAZEER (✉)2, Asifa HAMEED2, Jehanzeb FAROOQ3, Muhammad Rafiq SHAHID3 1 Sindh Agriculture University Tandojam, Pakistan 2 Cotton Research Station Multan, Pakistan 3 Cotton Research Institute Faisalabad, Pakistan ...

Genetics and Molecular Research, 2012

Abstract: Heading date in rice is one of the most important indices in rice breeding being govern... more Abstract: Heading date in rice is one of the most important indices in rice breeding being governed by multiple genes known as quantitative trait loci (QTLs). This character along with grain yield determines the commercial potential of any rice cultivar. In the last three decades the development of DNA markers have led to the precise analysis of QTLs. Various mapping populations are being used to explore QTLs for yield related parameters including heading date in rice. Like F 2, RILs, NILs, DH, BCs and SSSLs. To date among reported QTLs of heading date, 20 QTLs have been identified and cloned. The purpose of the present review is therefore to consolidate our existing knowledge about the progress that so far have been made in mapping of QTLs for heading date character. In addition to that requirements of QTL mapping and different QTL mapping methods have also been discussed. The information could be useful for further identification of QTLs for heading date that can be successfully i...

One hundred spring wheat accessions were assessed for heat tolerance under plastic sheet tunnel r... more One hundred spring wheat accessions were assessed for heat tolerance under plastic sheet tunnel resulting in seven parents with diverse heat tolerance. Tolerant and susceptible genotypes were graded on the bases of their relative cell injury percentages and relative values for different yield components. The analysis of variance for relative cell injury % revealed highly significant differences among the genotypes with a range from 28 to 98 %. These 7 parents were crossed in a full diallel system to evaluate the inheritance pattern of some spike related yield attributes (spike length, spikelets per spike, spike density, spike weight, grains per spike and grain yield per plant) under different temperature regimes at grain filling stage. Preliminary ANOVA revealed significant genotypic variation (P<0.01) for all the traits studied under both environments. Spike length, spikelets per spike and spike density revealed partial fitness of data for additive dominance model under normal c...

4 Abstract: Heading date in rice is one of the most important indices in rice breeding being gove... more 4 Abstract: Heading date in rice is one of the most important indices in rice breeding being governed by multiple genes known as quantitative trait loci (QTLs). This character along with grain yield determines the commercial potential of any rice cultivar. In the last three decades the development of DNA markers have led to the precise analysis of QTLs. Various mapping populations are being used to explore QTLs for yield related parameters including heading date in rice. Like F , RILs, NILs, DH, BCs and SSSLs. To date among reported QTLs of 2 heading date, 20 QTLs have been identified and cloned. The purpose of the present review is therefore to consolidate our existing knowledge about the progress that so far have been made in mapping of QTLs for heading date character. In addition to that requirements of QTL mapping and different QTL mapping methods have also been discussed. The information could be useful for further identification of QTLs for heading date that can be successfull...

African Journal of Biotechnology, 2011

The ability of plant to sustain itself in limited water conditions is crucial in the world of agr... more The ability of plant to sustain itself in limited water conditions is crucial in the world of agriculture. To breed for drought tolerance in wheat, it is essential to clearly understand drought tolerant mechanisms. Conventional breeding is time consuming and labor intensive being inefficient with low heritability traits like drought tolerance. Recent progress made in the field of genomics enabling us to access genes linked with drought tolerance has enhanced our understanding of this complex phenomenon. The purpose of this review paper was to briefly overview the accomplishments in molecular breeding for drought tolerance in wheat. Thus, by knowing the genetics of drought tolerance and identifying quantitative trait loci (QTLs) linked with DNA markers will help wheat breeders to develop high yielding drought tolerant cultivars. Key words: Triticum aestivum L, drought tolerance, QTLs, marker assisted selection (MAS).