Waseem Ali - Academia.edu (original) (raw)

Papers by Waseem Ali

Journal of social research development, Dec 25, 2022

Sustainable public-private partnership project performance is great challenge for project managem... more Sustainable public-private partnership project performance is great challenge for project management domain. This study aims to investigate relationship between critical success factors for public-private partnership & sustainable public-private partnership project performance. CSF theory has been used to evaluate relationship. Quantitative approach has been used to determine the link between the study variables. Cross-sectional data with a primary method for data collection is used. An adapted questionnaire is used to get the data. The correlation analysis between study variables provides empirical evidence about the significant relationship between CSFs and sustainable PPP project performance. The results therefore provide significant information in deciding about the desired relationship and reaching the conclusion. The study results can be used by researchers and policymakers for the sustainable PPP projects performance and based upon the relationship strength CSFs can be accorded preferences.

International Journal of Molecular Sciences

Our previous studies have confirmed that cadmium (Cd) exposure causes hepatotoxicity; it also ind... more Our previous studies have confirmed that cadmium (Cd) exposure causes hepatotoxicity; it also induces autophagy and blocks the autophagy flux. Therefore, we hypothesized that Cd hepatotoxicity could be alleviated through nutritional intervention. Taurine (Tau) has various biological functions such as acting as an antioxidant, acting as an anti-inflammatory, and stabilizing cell membranes. In order to explore the protective effect and internal mechanism of Tau on Cd-induced hepatotoxicity, normal rat liver cell line BRL3A cells were treated with Cd alone or in combination with Tau to detect cell injury and autophagy-related indexes in this study. We found that Tau can alleviate Cd-induced cell-proliferation decline and morphological changes in the cell. In addition, Tau activates autophagy and alleviates the blockage of Cd-induced autophagy flux. In this process, lysosome acidification and degradation were enhanced, and autophagosomes were further fused with lysosomes. Then, we found...

Background The widespread use of plastic products and the imperfection of plastic recycling syste... more Background The widespread use of plastic products and the imperfection of plastic recycling systems have led to a continuous increase in microplastics (PS) in the environment. Microplastics have an adsorption effect and can act as carriers for other pollutants in the environment. Cadmium (Cd) is a heavy metal that interacts with microplastics. However, the potential toxicity of co-exposure of cadmium and microplastics to the body is not clear. This study focuses on the effects of co-exposure to cadmium and microplastics on liver fibrosis and its mechanism. Results In this study investigated, Cd+PS exposure increased superoxide anion production and promoted extracellular ATP release compared with exposure to Cd or PS alone. Cd+PS increased inflammatory cell infiltration, activated the P2X7-NLRP3 signaling pathway, and promoted inflammatory factor release. Cd+PS aggravated Cd- or PS-induced liver fibrosis and induced liver inflammation. In AML12/HSC-T6 cell in vitropoisoning model, ex...

الجامعة الإسلامية - غزة, 2016

Additional file 4–39: All the RF and SVM models generated in present study for prediction of Ser,... more Additional file 4–39: All the RF and SVM models generated in present study for prediction of Ser, Thr and Tyr phosphorylation sites.

Additional file 2: The different types of features obtained after two-step, mRMR and SU, feature ... more Additional file 2: The different types of features obtained after two-step, mRMR and SU, feature selection approach for pS/pT/pY sites prediction.

Additional file 3: Confusion matrix for all the RF and SVM models generated in present study for ... more Additional file 3: Confusion matrix for all the RF and SVM models generated in present study for prediction of Ser, Thr and Tyr phosphorylation sites.

Additional file 5. Provide the performance measures for the RF and SVM models for each cardiovasc... more Additional file 5. Provide the performance measures for the RF and SVM models for each cardiovascular ADR using eighty features biological, chemical, phenotypic features and their two and three level combinations on non-redundant testing dataset.

Additional file 2. Provide the ten-fold cross-validation performance measures for the RF and SVM ... more Additional file 2. Provide the ten-fold cross-validation performance measures for the RF and SVM models for each cardiovascular ADR using biological, chemical, phenotypic features and their two and three level combinations.

Additional file 1. Provides the list of the top 50 features ranked by mRMR.

The present experiment was carried out at New Developmental farm, of the University of Agricultur... more The present experiment was carried out at New Developmental farm, of the University of Agriculture, Peshawar to study the effect of different rates of nitrogen, phosphorus fertilizers and irrigation on total N, NH4-N and NO3-N at various soil depths in maize crop (Zea mays L). The experimental design was randomize complete block design with split plot arrangement.Irrigation with two levels (Irrigation at the same day, Irrigation after 5 days) was kept in main plot whereas different fertilization treatments N (50, 100, 150 kg ha-1) and P (0, 60, 90 kg ha-1) were arranged in subplot.The results showed that the treatments combinations (N @ 150 kg ha-1 and P @ 90 kg ha-1) N3P3, (irrigation at the same day and N @ 150 kg ha-1) I1N3, and (irrigation at the same day and P @ 90 kg ha-1) I1P3 yielded maximum grain yield and biological yield. It was observed that among soil chemical properties total mineral nitrogen N, NH4-N and NO3-N concentration at upper soil surface, sub soil surface and at the depth (0-15cm) maximum values were recorded highest at treatments combinations N3P3(N @ 150 kg ha-1 and P @ 90 kg ha-1), I1N3(irrigation at the same day and N @ 150 kg ha-1) and I1P3(irrigation at the same day and P @ 90 kg ha-1) while the maximum losses were also recorded in the same treatment combinations. It was apparent from the present study that distribution of urea in the rooting zone has the potential to enhance N use efficiency and minimize N losses via ammonia volatilization. Moreover irrigation after application of Urea and Single superphosphate (SSP) is recommended which can cause these fertilizers to diffuse from upper surface to sub surface and can be easily uptake by plants consequently maximum yield components can be obtained.

Journal of Translational Medicine, 2021

Background Post-translational modification (PTM) is a biological process that alters proteins and... more Background Post-translational modification (PTM) is a biological process that alters proteins and is therefore involved in the regulation of various cellular activities and pathogenesis. Protein phosphorylation is an essential process and one of the most-studied PTMs: it occurs when a phosphate group is added to serine (Ser, S), threonine (Thr, T), or tyrosine (Tyr, Y) residue. Dysregulation of protein phosphorylation can lead to various diseases—most commonly neurological disorders, Alzheimer’s disease, and Parkinson’s disease—thus necessitating the prediction of S/T/Y residues that can be phosphorylated in an uncharacterized amino acid sequence. Despite a surplus of sequencing data, current experimental methods of PTM prediction are time-consuming, costly, and error-prone, so a number of computational methods have been proposed to replace them. However, phosphorylation prediction remains limited, owing to substrate specificity, performance, and the diversity of its features. Metho...

Cells

Cadmium is a heavy toxic metal with unknown biological functions in the human body. Over time, ca... more Cadmium is a heavy toxic metal with unknown biological functions in the human body. Over time, cadmium accretion in the different visceral organs (liver, lungs, kidney, and testis) is said to impair the function of these organs, which is associated with a relatively long biological half-life and a very low rate of excretion. Recently studies have revealed that the testes are highly sensitive to cadmium. In this review, we discussed the adverse effect of cadmium on the development and biological functions of the testis. The Sertoli cells (SCs), seminiferous tubules, and Blood Testis Barrier are severely structurally damaged by cadmium, which results in sperm loss. The development and function of Leydig cells are hindered by cadmium, which also induces Leydig cell tumors. The testis’s vascular system is severely disturbed by cadmium. Cadmium also perturbs the function of somatic cells and germ cells through epigenetic regulation, giving rise to infertile or sub-fertile males. In addit...

Journal of Medical Case Reports

Background Non-Hodgkin lymphoma is the fourth most common malignancy in children, and it is not c... more Background Non-Hodgkin lymphoma is the fourth most common malignancy in children, and it is not considered to be a hereditary disorder. However, it could affect members from the same family. Case presentation We are presenting two cases of Caucasian female siblings who were diagnosed with mediastinal lymphoblastic lymphoma in the same year. The two young females were presented to the emergency department with respiratory symptoms. After doing radiological investigations and biopsies, they were diagnosed with lymphoblastic lymphoma. The elder sister died before confirming the diagnosis, and the other is on chemotherapy now, with good treatment outcomes. Conclusions This case emphasizes the crucial role of precursor genetics in lymphoblastic lymphomas and suggests a strong relation between these genetics and age at symptom presentation. This is the first report of non-Hodgkin lymphoma in a pair of siblings in the pediatric population.

Scientific Reports

Tuberculosis (TB) is a leading cause of death worldwide and its impact has intensified due to the... more Tuberculosis (TB) is a leading cause of death worldwide and its impact has intensified due to the emergence of multi drug-resistant (MDR) and extensively drug-resistant (XDR) tB strains. protein phosphorylation plays a vital role in the virulence of Mycobacterium tuberculosis (M.tb) mediated by protein kinases. protein tyrosine phosphatase A (MptpA) undergoes phosphorylation by a unique tyrosine-specific kinase, protein tyrosine kinase A (PtkA), identified in the M.tb genome. ptkA phosphorylates PtpA on the tyrosine residues at positions 128 and 129, thereby increasing PtpA activity and promoting pathogenicity of MptpA. In the present study, we performed an extensive investigation of the conformational behavior of the intrinsically disordered domain (iDD) of ptkA using replica exchange molecular dynamics simulations. Long-term molecular dynamics (MD) simulations were performed to elucidate the role of iDD on the catalytic activity of kinase core domain (KcD) of ptkA. This was followed by identification of the probable inhibitors of PtkA using drug repurposing to block the PtpA-PtkA interaction. The inhibitory role of IDD on KCD has already been established; however, various analyses conducted in the present study showed that iDD ptkA had a greater inhibitory effect on the catalytic activity of KcD ptkA in the presence of the drugs esculin and inosine pranobex. the binding of drugs to PtkA resulted in formation of stable complexes, indicating that these two drugs are potentially useful as inhibitors of M.tb. Mycobacterium tuberculosis (M.tb) is an infectious agent that causes tuberculosis (TB) which is an airborne disease that affects almost one third of the world population. The Global Tuberculosis Report (2018), published by World Health Organization, estimated 1.3 million deaths and around 10 million new cases due to TB 1. The currently available treatments for TB include first-line drugs (isoniazid, rifampicin, ethambutol, and pyrazinamide), second-line injectable drugs (amikacin, kanamycin, and capreomycin), and fluoroquinolones in combination with the second-line drugs. Drug-resistant TB has emerged due to factors that include improper treatment; poor quality, limited supply and cost of drugs; person-to-person transmission of resistant bacteria; and poor compliance. Drug resistance is now a major obstacle to effective global TB management and prevention 2. In 2017, 30% of the 6.7 million new or TB relapse cases were reported to have resistance to rifampicin (the first-line drug of treatment) worldwide. Thus, the need of the hour is to develop novel, effective, and safer treatment strategies for the treatment of multidrug resistant (MDR) and extensively drug resistant (XDR) TB. However, the process of drug development, which includes a series of steps starting from pre-clinical studies to clinical trials, is time, cost, and labor extensive. This has resulted in a lack of new molecules that could be further developed and approved by the FDA for successful incorporation into anti-TB treatment strategies. Drug repurposing therefore emerges as a novel approach for drug development, as it merely involves discovering new targets for pre-existing drugs. Repurposing can be viewed as a highly effective, time-saving, and low risk drug development strategy, since the drug to be repurposed has already passed all the clinical trials 1. M.tb spreads via aerosols from infected individuals with active pulmonary disease. Transmission occurs when a person inhales droplet nuclei containing M.tb and the droplets reach the alveoli of the lungs. The bacteria are

Notulae Scientia Biologicae

Markers have been used over the years for the classification of plants. Markers are any trait of ... more Markers have been used over the years for the classification of plants. Markers are any trait of an organism that can be identified with confidence and relative easy, and can be followed in a mapping population on another hand markers be defined as heritable entities associated with the economically important trait under the control of polygenes. Morphological markers can be detected with naked eye (naked eye polymorphism) or as difference in physical or chemical properties of the macromolecules. In other words, there are two types of genetic markers viz. morphological markers or naked eye polymorphism and non-morphological markers or molecular markers. Morphological markers include traits such as plant height, disease response, photoperiod, sensitivity, shape or colour of flowers, fruits or seeds etc. Molecular markers include biochemical constituents. Morphological markers have many limitations for being used as markers particularly in fruit crops because of long generation time a...

Journal of Translational Medicine

Background: Predicting adverse drug reactions (ADRs) has become very important owing to the huge ... more Background: Predicting adverse drug reactions (ADRs) has become very important owing to the huge global health burden and failure of drugs. This indicates a need for prior prediction of probable ADRs in preclinical stages which can improve drug failures and reduce the time and cost of development thus providing efficient and safer therapeutic options for patients. Though several approaches have been put forward for in silico ADR prediction, there is still room for improvement. Methods: In the present work, we have used machine learning based approach for cardiovascular (CV) ADRs prediction by integrating different features of drugs, biological (drug transporters, targets and enzymes), chemical (substructure fingerprints) and phenotypic (therapeutic indications and other identified ADRs), and their two and three level combinations. To recognize quality and important features, we used minimum redundancy maximum relevance approach while synthetic minority over-sampling technique balancing method was used to introduce a balance in the training sets. Results: This is a rigorous and comprehensive study which involved the generation of a total of 504 computational models for 36 CV ADRs using two state-of-the-art machine-learning algorithms: random forest and sequential minimization optimization. All the models had an accuracy of around 90% and the biological and chemical features models were more informative as compared to the models generated using chemical features. Conclusions: The results obtained demonstrated that the predictive models generated in the present study were highly accurate, and the phenotypic information of the drugs played the most important role in drug ADRs prediction. Furthermore, the results also showed that using the proposed method, different drugs properties can be combined to build computational predictive models which can effectively predict potential ADRs during early stages of drug development.

Microbial pathogenesis, Jan 26, 2018

It is conceivable that early developing germ cells must across the basal to the luminal region of... more It is conceivable that early developing germ cells must across the basal to the luminal region of seminiferous tubules (STs) during spermatogenesis is associated with extensive restructuring of junctional complex. However, very limited information is documented about these junctional complexes in reptiles. In the present study we have determined the localization of inter-Sertoli cell tight junctions (TJ's), protein CLDN11 and gap junction protein Cx43 during spermatogenesis in the testis. In early spermatogenesis, weak immunoreactivity of CLDN11and focal localization of Cx43 was observed around the Sertoli cell in the luminal region, but completely delaminated from the basal compartment of STs. In late spermatogenesis, strong focal to linear localization of CLDN11and Cx43 was detected at the points of contact between two Sertoli cells and around the early stages of primary spermatocytes in the basal compartment of STs. In late spermatogenesis, localization of CLDN11and Cx43 was ...

Journal of the College of Physicians and Surgeons Pakistan Jcpsp, May 1, 2007

The authors report the case of a previously healthy 10-year-old boy who accidentally ingested a t... more The authors report the case of a previously healthy 10-year-old boy who accidentally ingested a toothpick. He presented to the hospital 7 days after the incident with left lower quadrant abdominal pain and fever; there was evidence of localized peritoneal irritation by exam. He had mild leukocytosis. An abdominal CT scan showed the toothpick in the sigmoid colon with extension through the bowel wall. Laparoscopic exploration was undertaken and an 8-cm toothpick was identified and removed from the perforation site in the sigmoid colon. Via a small suprapubic incision the sigmoid was delivered and repaired. The patient had an uneventful recovery and was discharged home 2 days after the procedure. Laparoscopic exploration can be diagnostic and therapeutic in the management of ingested foreign bodies with suspected intestinal perforation.