Weibo Song - Academia.edu (original) (raw)

Papers by Weibo Song

Research paper thumbnail of Morphological studies on two marine colepid ciliates from Qingdao, China, Nolandia orientalis spec. nov. and Pinacocoleps similis (Kahl, 1933) comb. nov. (Ciliophora, Colepidae)

European Journal of Protistology, Nov 1, 2010

Recent studies have revealed that the "lower" marine ciliates are far more diverse than previousl... more Recent studies have revealed that the "lower" marine ciliates are far more diverse than previously suspected. During a survey on the ciliate fauna in coastal waters of Qingdao, northern China, we have isolated about 30 new or poorly known taxa. In the present study two colepid species are investigated, Nolandia orientalis spec. nov. and Pinacocoleps similis (Kahl, 1933) comb. nov. (basionym: Coleps similis Kahl, 1933). Their morphology and infraciliature are documented based on living observations and silver impregnations. The new species Nolandia orientalis differs from the type species N. nolandi mainly in the structure of tier plates. The structure of the tier plates was also the basis for transferring Coleps similis Kahl, 1933 to the genus Pinacocoleps and for three further new combinations:

Research paper thumbnail of Taxonomic descriptions of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec. nov. and Tiarina fusa (Claparède & Lachmann, 1858) Bergh, 1881 (Ciliophora, Prorodontida)

International Journal of Systematic and Evolutionary Microbiology, Mar 1, 2012

The morphology of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec.... more The morphology of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec. nov. and Tiarina fusa (Claparè de & Lachmann, 1858) Bergh, 1881, isolated from Chinese coastal waters, was investigated. N. sinica spec. nov. may be separated from its congeners by the structure of its armour plates, each of which may have up to five reniform windows. A. caoi spec. nov. is characterized by its large body with broad anterior end and by having 10-12 long, sharp posterior spines. New data and an improved diagnosis are supplied for Tiarina fusa (Claparè de & Lachmann, 1858) Bergh, 1881, which has a spindle-shaped body, about 16 ciliary rows and a single adoral organelle. Sequence similarities with other available colepid species were determined. METHODS Nolandia sinica spec. nov. was collected in May 2007 and Apocoleps caoi spec. nov. in June 2007, from the intertidal regions of sandy beaches near Jiaozhou Bay, Qingdao (36u 069 N 120u 299 E), China. The upper 15 cm layer of sand was collected together with seawater from the site. Raw cultures were maintained for one or two weeks at room temperature (Wang et al., 2008a, b). Tiarina fusa (Claparède & Lachmann, 1858) Bergh, 1881 was collected in October 2007 using 20 mm plankton nets from coastal waters of Daya Bay, near Shenzhen (22u 369 30 N 114u 329 460 E), China.

Research paper thumbnail of Two novel marine Frontonia species, Frontonia mengi spec. nov. and Frontonia magna spec. nov. (Protozoa; Ciliophora), with notes on their phylogeny based on small-subunit rRNA gene sequence data

International Journal of Systematic and Evolutionary Microbiology, Jun 1, 2011

The morphology and infraciliature of two novel marine ciliates, Frontonia mengi spec. nov. and Fr... more The morphology and infraciliature of two novel marine ciliates, Frontonia mengi spec. nov. and Frontonia magna spec. nov., isolated from coastal waters in northern and southern China, respectively, were investigated using living observation and silver impregnation methods. Frontonia mengi spec. nov. is characterized by its slender body shape, with a length-to-width ratio of about 5 : 1, about 52 somatic kineties and the structure of its buccal apparatus. Frontonia magna spec. nov. can be recognized by the combination of huge body size, about 200 somatic kineties, five or six vestibular kineties and four-rowed peniculi 1-3. Phylogenetic trees based on small-subunit rRNA gene sequences were constructed by means of Bayesian inference and maximum-parsimony. Results showed that F. mengi and F. magna are sister to each other within the 'core' clade of Frontonia that also includes Frontonia lynni and Frontonia tchibisovae and that the genus Frontonia may be polyphyletic, because one species, Frontonia didieri, always groups with Paramecium and Apofrontonia.

Research paper thumbnail of Taxonomy and phylogeny of three heterotrich ciliates (Protozoa, Ciliophora), with description of a new<i>B</i><i>lepharisma</i>species

Zoological Journal of the Linnean Society, May 20, 2016

The morphology and phylogeny of three heterotrich ciliates, Anigsteinia clarissima (Anigstein, 19... more The morphology and phylogeny of three heterotrich ciliates, Anigsteinia clarissima (Anigstein, 1912) Isquith, 1968, Blepharisma penardi sp. nov., and Blepharisma undulans Stein, 1867, were investigated based on living morphology, infraciliature, and small subunit (SSU) rDNA sequence data. The new species B. penardi sp. nov. is recognized by the following combination of characters: size about 150-180 × 45-55 μm in vivo, cell colour variable from colourless to pale pink to dark brownish; peristome extending to middle of body; 36-63 adoral membranelles; 24-34 somatic kineties; single macronucleus; cortical granules tiny and colourless; freshwater habitat. Anigsteinia clarissima and B. undulans are both reported from China for the first time and are redescribed based on a combination of previous descriptions and new data from the Chinese populations. Phylogenetic analyses based on SSU rDNA sequence data show that B. penardi sp. nov. and B. undulans are both located within a clade comprising only congeners, thus supporting the monophyly of the genus Blepharisma. Anigsteinia clarissima clusters with its only congener forming a clade that is sister to the Spirostomum assemblage. Both the morphological and the molecular data support the placement of Anigsteinia in the family Spirostomidae.

Research paper thumbnail of Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., with improved definition of Pseudoplatynematum (Ciliophora, Oligohymenophora)

European Journal of Protistology, Aug 1, 2010

Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., i... more Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., isolated from coastal waters of Qingdao, northern China, were investigated using live observation and silver impregnations. Sathrophilus planus can be recognized by its elongate and conspicuously flattened body, ca. 16 somatic kineties, single postoral kinety, extremely elongated first kinety row of membranelle 1, and its marine habitat. Among these features, the structure of membranelle 1 is the most characteristic and enables this species to be easily distinguished from its congeners. Pseudoplatynematum dengi is characterized by the following features: cell surface conspicuously strengthened and notched; prominent spines both at anterior and posterior ends of cell; membranelles 1 and 2 three-rowed, membranelle 3 single-rowed; paroral membrane composed of two parts, anterior end of upper part extending to about level of membranelle 2; ca. 20 somatic kineties, one postoral kinety; single prolonged caudal cilium; contractile vacuole caudally positioned. The diagnosis of the genus Pseudoplatynematum was renewed to include some new characteristics revealed by silver impregnation methods.

Research paper thumbnail of 形態学的および分子分析に基づく2つの繊毛虫属(繊毛虫類,Heterotrichea)に対する新たなGruberia種の記述による新たな寄与【JST・京大機械翻訳】

Research paper thumbnail of Taxonomic studies on a new marine ciliate, Apocoleps magnus gen. nov., spec. nov. (Ciliophora, Colepidae), isolated from Qingdao, China

Journal of Ocean University of China, Oct 25, 2009

ABSTRACT

Research paper thumbnail of Morphology and infraciliature of the oligotrich ciliate Strombidium rapulum (Yagiu, 1933) Kahl, 1934 (Protozoa, Ciliophora, Oligotrichida) from the intestine of sea urchin Hemicentrotus pulcherrimus Agassiz

Zootaxa, Jan 24, 2006

The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Y... more The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Yagiu, 1933) Kahl, 1934, isolated from the intestine of the sea urchin Hemicentrotus pulcherrimus Agassiz, was investigated based on observations of specimens from living cells and fixed protargol impregnated cells. An improved diagnosis is given based on previous and current studies: large sized marine Strombidium in vivo 50-130 × 25-65 µm; dorsoventrally slightly flattened; outline ovate and turnip-shaped, tapering posteriorly and terminating in an elongate tail; apical protrusion about 5 µm long in live cells; one elongated macronucleus; girdle kinety equatorial composed of about 57 monokinetids; ventral kinety located subcaudally on right margin, composed of about 5 dikinetids; on average 16 anterior and 29 ventral membranelles.

Research paper thumbnail of New contributions to the phylogeny of the ciliate class Heterotrichea (Protista, Ciliophora): analyses at family-genus level and new evolutionary hypotheses

Science China-life Sciences, Oct 13, 2020

Heterotrichous ciliates play an important role in aquatic ecosystem energy flow processes and man... more Heterotrichous ciliates play an important role in aquatic ecosystem energy flow processes and many are model organisms for research in cytology, regenerative biology, and toxicology. In the present study, we combine both morphological and molecular data to infer phylogenetic relationships at family-genus level and propose new evolutionary hypotheses for the class Heterotrichea. The main results include: (1) 96 new ribosomal DNA sequences from 36 populations, representing eight families and 13 genera, including three poorly annotated genera, Folliculinopsis, Ampullofolliculina and Linostomella; (2) the earliest-branching families are Spirostomidae in single-gene trees and Peritromidae in the concatenated tree, but the family Peritromidae probably represents the basal lineage based on its possession of many "primitive" morphological characters; (3) some findings in molecular trees are not supported by morphological evidence, such as the family Blepharismidae is one of the most recent branches and the relationship between Fabreidae and Folliculinidae is very close; (4) the systematic positions of Condylostomatidae, Climacostomidae, and Gruberiidae remain uncertain based either on morphological or molecular data; and (5) the monophyly of each genus included in the present study is supported by the molecular phylogenetic trees, except for Blepharisma in the SSU rDNA tree and Folliculina in the ITS1-5.8S-ITS2 tree.

Research paper thumbnail of Redescriptions of Two Poorly Known Marine Suctorian Ciliates, Ephelota truncata Fraipont, 1878 and Ephelota mammillata Dons, 1918 (Protozoa, Ciliophora, Suctoria), from Qingdao, China

Acta Protozoologica, 2008

Summary. Two poorly known marine suctorian ciliates, Ephelota truncata Fraipont, 1878 and Ephelot... more Summary. Two poorly known marine suctorian ciliates, Ephelota truncata Fraipont, 1878 and Ephelota mammillata Dons, 1918, collected from the coastal waters off Qingdao, China, were investigated using both live observations and protargol impregation methods. Improved ...

Research paper thumbnail of A new contribution to the taxonomy and phylogeny of the ciliate genus Spirostomum (Alveolata, Ciliophora, Heterotrichea), with comprehensive descriptions of two species from wetlands in China

Water Biology and Security, 2022

Research paper thumbnail of Anteholosticha scutellum ( Cohn , 1866 ) Berger , 2003 , marine ciliates ( Ciliophora , Hypotrichida ) from tropical waters in southern China , with notes on their small-subunit rRNA gene sequences

Few studies using modern methods have been carried out on ciliated protozoa in tropical marine wa... more Few studies using modern methods have been carried out on ciliated protozoa in tropical marine waters. In the present work, two hypotrichs, Parabirojimia multinucleata spec. nov. and Anteholosticha scutellum (Cohn, 1866) Berger, 2003, collected from Daya Bay in southern China, were investigated morphologically. P. multinucleata is distinguished by the following combination of characters: slender body, without a snout-like protrusion in the frontal field, and about 50 macronuclear nodules. The poorly known A. scutellum has never been investigated using modern methods; hence, a redescription is needed. During the present study, observations of specimens in vivo and following protargol impregnation revealed new information concerning structures such as the cortical granules and the infraciliature. A redescription and improved diagnosis are supplied based on the China population. The small-subunit (SSU) rRNA gene was sequenced for both organisms and comparisons with those of similar con...

Research paper thumbnail of New contribution to the morphological taxonomy of three marine cyrtophorid ciliates from the Yellow Sea, China (Ciliophora: Cyrtophorida)

Acta Protozoologica, 2011

The morphology and infraciliature of three marine cyrtophorid ciliates, Dysteria lanceolata Clapa... more The morphology and infraciliature of three marine cyrtophorid ciliates, Dysteria lanceolata Claparede and Lachmann, 1859, Lynchella nordica Jankowski, 1968 and Chlamydonyx paucidentatus Deroux, 1976, collected from the coastal waters of Qingdao, China, were investigated using live observations and the protargol impregnation method. D. lanceolata was oval in body outline, about 65 x 45 mu m in vivo, with a subcaudally positioned podite, six to seven right kineties, two or three frontoventral kineties, and two ventral contractile vacuoles. The improved diagnosis for L. nordica and a key to all known Lynchella species are supplied. The rediscovery of C. paucidentatus enables us to accept its taxonomic identification (e.g., separation from a morphologically similar species Trochilioides recta). The genus Trochilioides Deroux, nov. gen., which was a nomen nudum according to ICZN (1999), is re-established.

Research paper thumbnail of Phylogeny of a new ciliate family Clampidae fam. nov. (Protista: Ciliophora), with notes on morphology and morphogenesis

Zoological Journal of the Linnean Society, 2021

Hypotrichs comprise a highly differentiated and diversified group of ciliates with an evolutionar... more Hypotrichs comprise a highly differentiated and diversified group of ciliates with an evolutionary history characterized by an exceptional number of convergences and reversions. A new hypotrich ciliate from a freshwater fishpond is investigated based on morphological, morphogenetic and molecular data. The phylogenetic analyses reveal that the new species is closely related to Simplicitergida despite the absence of zigzag-patterned midventral cirral pairs, a common feature of simplicitergids. The phylogenetic position of this atypical simplicitergid species is strongly supported by the conserved features of dorsal ciliature and nuclear apparatus, which are probably more informative than cirral patterns for higher-rank classification. The close phylogenetic relationships among those Simplicitergida with varied cirral patterns could be explained by evolutionary convergences and reversions. The new species is recognized mainly by three frontoventral rows, several short midventral rows, ...

Research paper thumbnail of New discoveries of the genus Thuricola Kent, 1881 (Ciliophora, Peritrichia, Vaginicolidae), with descriptions of three poorly known species from China

Acta Protozoologica, 2018

Research paper thumbnail of Morphology and infraciliature of the oligotrich ciliate Strombidium rapulum (Yagiu, 1933) Kahl, 1934 (Protozoa, Ciliophora, Oligotrichida) from the intestine of sea urchin Hemicentrotus pulcherrimus Agassiz

Zootaxa, 2006

The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Y... more The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Yagiu, 1933) Kahl, 1934, isolated from the intestine of the sea urchin Hemicentrotus pulcherrimus Agassiz, was investigated based on observations of specimens from living cells and fixed protargol impregnated cells. An improved diagnosis is given based on previous and current studies: large sized marine Strombidium in vivo 50–130 × 25–65 µm; dorsoventrally slightly flattened; outline ovate and turnip-shaped, tapering posteriorly and terminating in an elongate tail; apical protrusion about 5 µm long in live cells; one elongated macronucleus; girdle kinety equatorial composed of about 57 monokinetids; ventral kinety located subcaudally on right margin, composed of about 5 dikinetids; on average 16 anterior and 29 ventral membranelles.

Research paper thumbnail of Zootaxa

Oligotrichida) from the intestine of sea urchin

Research paper thumbnail of Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis

Zoologica Scripta, 2011

... n. was collected from the estuary of the Pearl River (113°41¢E; 22°38¢N) south of Guangzhou, ... more ... n. was collected from the estuary of the Pearl River (113°41¢E; 22°38¢N) south of Guangzhou, China ... amplified with the eukaryotic universal SSU rRNA primers EukA (5¢- AACCTGGTTGATCCTGCCAGT-3¢) and EukB (5¢-TGATCCTTCTGCAGGTTCACCTAC-3¢) ( ...

Research paper thumbnail of Taxonomic studies on a new marine ciliate, Apocoleps magnus gen. nov., spec. nov. (Ciliophora, Colepidae), isolated from Qingdao, China

Journal of Ocean University of China, 2009

ABSTRACT

Research paper thumbnail of A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species

Journal of Natural History, 2008

Twenty-eight littoral ciliates including one new genus and seven new species were collected from ... more Twenty-eight littoral ciliates including one new genus and seven new species were collected from King George Island, Antarctica. Among them, 21 species are morphologically and taxonomically described in this paper: Bickella antarctica nov. gen., nov. spec.,

Research paper thumbnail of Morphological studies on two marine colepid ciliates from Qingdao, China, Nolandia orientalis spec. nov. and Pinacocoleps similis (Kahl, 1933) comb. nov. (Ciliophora, Colepidae)

European Journal of Protistology, Nov 1, 2010

Recent studies have revealed that the "lower" marine ciliates are far more diverse than previousl... more Recent studies have revealed that the "lower" marine ciliates are far more diverse than previously suspected. During a survey on the ciliate fauna in coastal waters of Qingdao, northern China, we have isolated about 30 new or poorly known taxa. In the present study two colepid species are investigated, Nolandia orientalis spec. nov. and Pinacocoleps similis (Kahl, 1933) comb. nov. (basionym: Coleps similis Kahl, 1933). Their morphology and infraciliature are documented based on living observations and silver impregnations. The new species Nolandia orientalis differs from the type species N. nolandi mainly in the structure of tier plates. The structure of the tier plates was also the basis for transferring Coleps similis Kahl, 1933 to the genus Pinacocoleps and for three further new combinations:

Research paper thumbnail of Taxonomic descriptions of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec. nov. and Tiarina fusa (Claparède & Lachmann, 1858) Bergh, 1881 (Ciliophora, Prorodontida)

International Journal of Systematic and Evolutionary Microbiology, Mar 1, 2012

The morphology of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec.... more The morphology of three marine colepid ciliates, Nolandia sinica spec. nov., Apocoleps caoi spec. nov. and Tiarina fusa (Claparè de & Lachmann, 1858) Bergh, 1881, isolated from Chinese coastal waters, was investigated. N. sinica spec. nov. may be separated from its congeners by the structure of its armour plates, each of which may have up to five reniform windows. A. caoi spec. nov. is characterized by its large body with broad anterior end and by having 10-12 long, sharp posterior spines. New data and an improved diagnosis are supplied for Tiarina fusa (Claparè de & Lachmann, 1858) Bergh, 1881, which has a spindle-shaped body, about 16 ciliary rows and a single adoral organelle. Sequence similarities with other available colepid species were determined. METHODS Nolandia sinica spec. nov. was collected in May 2007 and Apocoleps caoi spec. nov. in June 2007, from the intertidal regions of sandy beaches near Jiaozhou Bay, Qingdao (36u 069 N 120u 299 E), China. The upper 15 cm layer of sand was collected together with seawater from the site. Raw cultures were maintained for one or two weeks at room temperature (Wang et al., 2008a, b). Tiarina fusa (Claparède & Lachmann, 1858) Bergh, 1881 was collected in October 2007 using 20 mm plankton nets from coastal waters of Daya Bay, near Shenzhen (22u 369 30 N 114u 329 460 E), China.

Research paper thumbnail of Two novel marine Frontonia species, Frontonia mengi spec. nov. and Frontonia magna spec. nov. (Protozoa; Ciliophora), with notes on their phylogeny based on small-subunit rRNA gene sequence data

International Journal of Systematic and Evolutionary Microbiology, Jun 1, 2011

The morphology and infraciliature of two novel marine ciliates, Frontonia mengi spec. nov. and Fr... more The morphology and infraciliature of two novel marine ciliates, Frontonia mengi spec. nov. and Frontonia magna spec. nov., isolated from coastal waters in northern and southern China, respectively, were investigated using living observation and silver impregnation methods. Frontonia mengi spec. nov. is characterized by its slender body shape, with a length-to-width ratio of about 5 : 1, about 52 somatic kineties and the structure of its buccal apparatus. Frontonia magna spec. nov. can be recognized by the combination of huge body size, about 200 somatic kineties, five or six vestibular kineties and four-rowed peniculi 1-3. Phylogenetic trees based on small-subunit rRNA gene sequences were constructed by means of Bayesian inference and maximum-parsimony. Results showed that F. mengi and F. magna are sister to each other within the 'core' clade of Frontonia that also includes Frontonia lynni and Frontonia tchibisovae and that the genus Frontonia may be polyphyletic, because one species, Frontonia didieri, always groups with Paramecium and Apofrontonia.

Research paper thumbnail of Taxonomy and phylogeny of three heterotrich ciliates (Protozoa, Ciliophora), with description of a new<i>B</i><i>lepharisma</i>species

Zoological Journal of the Linnean Society, May 20, 2016

The morphology and phylogeny of three heterotrich ciliates, Anigsteinia clarissima (Anigstein, 19... more The morphology and phylogeny of three heterotrich ciliates, Anigsteinia clarissima (Anigstein, 1912) Isquith, 1968, Blepharisma penardi sp. nov., and Blepharisma undulans Stein, 1867, were investigated based on living morphology, infraciliature, and small subunit (SSU) rDNA sequence data. The new species B. penardi sp. nov. is recognized by the following combination of characters: size about 150-180 × 45-55 μm in vivo, cell colour variable from colourless to pale pink to dark brownish; peristome extending to middle of body; 36-63 adoral membranelles; 24-34 somatic kineties; single macronucleus; cortical granules tiny and colourless; freshwater habitat. Anigsteinia clarissima and B. undulans are both reported from China for the first time and are redescribed based on a combination of previous descriptions and new data from the Chinese populations. Phylogenetic analyses based on SSU rDNA sequence data show that B. penardi sp. nov. and B. undulans are both located within a clade comprising only congeners, thus supporting the monophyly of the genus Blepharisma. Anigsteinia clarissima clusters with its only congener forming a clade that is sister to the Spirostomum assemblage. Both the morphological and the molecular data support the placement of Anigsteinia in the family Spirostomidae.

Research paper thumbnail of Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., with improved definition of Pseudoplatynematum (Ciliophora, Oligohymenophora)

European Journal of Protistology, Aug 1, 2010

Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., i... more Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., isolated from coastal waters of Qingdao, northern China, were investigated using live observation and silver impregnations. Sathrophilus planus can be recognized by its elongate and conspicuously flattened body, ca. 16 somatic kineties, single postoral kinety, extremely elongated first kinety row of membranelle 1, and its marine habitat. Among these features, the structure of membranelle 1 is the most characteristic and enables this species to be easily distinguished from its congeners. Pseudoplatynematum dengi is characterized by the following features: cell surface conspicuously strengthened and notched; prominent spines both at anterior and posterior ends of cell; membranelles 1 and 2 three-rowed, membranelle 3 single-rowed; paroral membrane composed of two parts, anterior end of upper part extending to about level of membranelle 2; ca. 20 somatic kineties, one postoral kinety; single prolonged caudal cilium; contractile vacuole caudally positioned. The diagnosis of the genus Pseudoplatynematum was renewed to include some new characteristics revealed by silver impregnation methods.

Research paper thumbnail of 形態学的および分子分析に基づく2つの繊毛虫属(繊毛虫類,Heterotrichea)に対する新たなGruberia種の記述による新たな寄与【JST・京大機械翻訳】

Research paper thumbnail of Taxonomic studies on a new marine ciliate, Apocoleps magnus gen. nov., spec. nov. (Ciliophora, Colepidae), isolated from Qingdao, China

Journal of Ocean University of China, Oct 25, 2009

ABSTRACT

Research paper thumbnail of Morphology and infraciliature of the oligotrich ciliate Strombidium rapulum (Yagiu, 1933) Kahl, 1934 (Protozoa, Ciliophora, Oligotrichida) from the intestine of sea urchin Hemicentrotus pulcherrimus Agassiz

Zootaxa, Jan 24, 2006

The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Y... more The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Yagiu, 1933) Kahl, 1934, isolated from the intestine of the sea urchin Hemicentrotus pulcherrimus Agassiz, was investigated based on observations of specimens from living cells and fixed protargol impregnated cells. An improved diagnosis is given based on previous and current studies: large sized marine Strombidium in vivo 50-130 × 25-65 µm; dorsoventrally slightly flattened; outline ovate and turnip-shaped, tapering posteriorly and terminating in an elongate tail; apical protrusion about 5 µm long in live cells; one elongated macronucleus; girdle kinety equatorial composed of about 57 monokinetids; ventral kinety located subcaudally on right margin, composed of about 5 dikinetids; on average 16 anterior and 29 ventral membranelles.

Research paper thumbnail of New contributions to the phylogeny of the ciliate class Heterotrichea (Protista, Ciliophora): analyses at family-genus level and new evolutionary hypotheses

Science China-life Sciences, Oct 13, 2020

Heterotrichous ciliates play an important role in aquatic ecosystem energy flow processes and man... more Heterotrichous ciliates play an important role in aquatic ecosystem energy flow processes and many are model organisms for research in cytology, regenerative biology, and toxicology. In the present study, we combine both morphological and molecular data to infer phylogenetic relationships at family-genus level and propose new evolutionary hypotheses for the class Heterotrichea. The main results include: (1) 96 new ribosomal DNA sequences from 36 populations, representing eight families and 13 genera, including three poorly annotated genera, Folliculinopsis, Ampullofolliculina and Linostomella; (2) the earliest-branching families are Spirostomidae in single-gene trees and Peritromidae in the concatenated tree, but the family Peritromidae probably represents the basal lineage based on its possession of many "primitive" morphological characters; (3) some findings in molecular trees are not supported by morphological evidence, such as the family Blepharismidae is one of the most recent branches and the relationship between Fabreidae and Folliculinidae is very close; (4) the systematic positions of Condylostomatidae, Climacostomidae, and Gruberiidae remain uncertain based either on morphological or molecular data; and (5) the monophyly of each genus included in the present study is supported by the molecular phylogenetic trees, except for Blepharisma in the SSU rDNA tree and Folliculina in the ITS1-5.8S-ITS2 tree.

Research paper thumbnail of Redescriptions of Two Poorly Known Marine Suctorian Ciliates, Ephelota truncata Fraipont, 1878 and Ephelota mammillata Dons, 1918 (Protozoa, Ciliophora, Suctoria), from Qingdao, China

Acta Protozoologica, 2008

Summary. Two poorly known marine suctorian ciliates, Ephelota truncata Fraipont, 1878 and Ephelot... more Summary. Two poorly known marine suctorian ciliates, Ephelota truncata Fraipont, 1878 and Ephelota mammillata Dons, 1918, collected from the coastal waters off Qingdao, China, were investigated using both live observations and protargol impregation methods. Improved ...

Research paper thumbnail of A new contribution to the taxonomy and phylogeny of the ciliate genus Spirostomum (Alveolata, Ciliophora, Heterotrichea), with comprehensive descriptions of two species from wetlands in China

Water Biology and Security, 2022

Research paper thumbnail of Anteholosticha scutellum ( Cohn , 1866 ) Berger , 2003 , marine ciliates ( Ciliophora , Hypotrichida ) from tropical waters in southern China , with notes on their small-subunit rRNA gene sequences

Few studies using modern methods have been carried out on ciliated protozoa in tropical marine wa... more Few studies using modern methods have been carried out on ciliated protozoa in tropical marine waters. In the present work, two hypotrichs, Parabirojimia multinucleata spec. nov. and Anteholosticha scutellum (Cohn, 1866) Berger, 2003, collected from Daya Bay in southern China, were investigated morphologically. P. multinucleata is distinguished by the following combination of characters: slender body, without a snout-like protrusion in the frontal field, and about 50 macronuclear nodules. The poorly known A. scutellum has never been investigated using modern methods; hence, a redescription is needed. During the present study, observations of specimens in vivo and following protargol impregnation revealed new information concerning structures such as the cortical granules and the infraciliature. A redescription and improved diagnosis are supplied based on the China population. The small-subunit (SSU) rRNA gene was sequenced for both organisms and comparisons with those of similar con...

Research paper thumbnail of New contribution to the morphological taxonomy of three marine cyrtophorid ciliates from the Yellow Sea, China (Ciliophora: Cyrtophorida)

Acta Protozoologica, 2011

The morphology and infraciliature of three marine cyrtophorid ciliates, Dysteria lanceolata Clapa... more The morphology and infraciliature of three marine cyrtophorid ciliates, Dysteria lanceolata Claparede and Lachmann, 1859, Lynchella nordica Jankowski, 1968 and Chlamydonyx paucidentatus Deroux, 1976, collected from the coastal waters of Qingdao, China, were investigated using live observations and the protargol impregnation method. D. lanceolata was oval in body outline, about 65 x 45 mu m in vivo, with a subcaudally positioned podite, six to seven right kineties, two or three frontoventral kineties, and two ventral contractile vacuoles. The improved diagnosis for L. nordica and a key to all known Lynchella species are supplied. The rediscovery of C. paucidentatus enables us to accept its taxonomic identification (e.g., separation from a morphologically similar species Trochilioides recta). The genus Trochilioides Deroux, nov. gen., which was a nomen nudum according to ICZN (1999), is re-established.

Research paper thumbnail of Phylogeny of a new ciliate family Clampidae fam. nov. (Protista: Ciliophora), with notes on morphology and morphogenesis

Zoological Journal of the Linnean Society, 2021

Hypotrichs comprise a highly differentiated and diversified group of ciliates with an evolutionar... more Hypotrichs comprise a highly differentiated and diversified group of ciliates with an evolutionary history characterized by an exceptional number of convergences and reversions. A new hypotrich ciliate from a freshwater fishpond is investigated based on morphological, morphogenetic and molecular data. The phylogenetic analyses reveal that the new species is closely related to Simplicitergida despite the absence of zigzag-patterned midventral cirral pairs, a common feature of simplicitergids. The phylogenetic position of this atypical simplicitergid species is strongly supported by the conserved features of dorsal ciliature and nuclear apparatus, which are probably more informative than cirral patterns for higher-rank classification. The close phylogenetic relationships among those Simplicitergida with varied cirral patterns could be explained by evolutionary convergences and reversions. The new species is recognized mainly by three frontoventral rows, several short midventral rows, ...

Research paper thumbnail of New discoveries of the genus Thuricola Kent, 1881 (Ciliophora, Peritrichia, Vaginicolidae), with descriptions of three poorly known species from China

Acta Protozoologica, 2018

Research paper thumbnail of Morphology and infraciliature of the oligotrich ciliate Strombidium rapulum (Yagiu, 1933) Kahl, 1934 (Protozoa, Ciliophora, Oligotrichida) from the intestine of sea urchin Hemicentrotus pulcherrimus Agassiz

Zootaxa, 2006

The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Y... more The morphology and infraciliature of a poorly known endocommensal ciliate, Strombidium rapulum (Yagiu, 1933) Kahl, 1934, isolated from the intestine of the sea urchin Hemicentrotus pulcherrimus Agassiz, was investigated based on observations of specimens from living cells and fixed protargol impregnated cells. An improved diagnosis is given based on previous and current studies: large sized marine Strombidium in vivo 50–130 × 25–65 µm; dorsoventrally slightly flattened; outline ovate and turnip-shaped, tapering posteriorly and terminating in an elongate tail; apical protrusion about 5 µm long in live cells; one elongated macronucleus; girdle kinety equatorial composed of about 57 monokinetids; ventral kinety located subcaudally on right margin, composed of about 5 dikinetids; on average 16 anterior and 29 ventral membranelles.

Research paper thumbnail of Zootaxa

Oligotrichida) from the intestine of sea urchin

Research paper thumbnail of Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis

Zoologica Scripta, 2011

... n. was collected from the estuary of the Pearl River (113°41¢E; 22°38¢N) south of Guangzhou, ... more ... n. was collected from the estuary of the Pearl River (113°41¢E; 22°38¢N) south of Guangzhou, China ... amplified with the eukaryotic universal SSU rRNA primers EukA (5¢- AACCTGGTTGATCCTGCCAGT-3¢) and EukB (5¢-TGATCCTTCTGCAGGTTCACCTAC-3¢) ( ...

Research paper thumbnail of Taxonomic studies on a new marine ciliate, Apocoleps magnus gen. nov., spec. nov. (Ciliophora, Colepidae), isolated from Qingdao, China

Journal of Ocean University of China, 2009

ABSTRACT

Research paper thumbnail of A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species

Journal of Natural History, 2008

Twenty-eight littoral ciliates including one new genus and seven new species were collected from ... more Twenty-eight littoral ciliates including one new genus and seven new species were collected from King George Island, Antarctica. Among them, 21 species are morphologically and taxonomically described in this paper: Bickella antarctica nov. gen., nov. spec.,