Wen-Wei Tseng - Academia.edu (original) (raw)

Papers by Wen-Wei Tseng

Biophysical journal, Feb 1, 2024

2022 IEEE 22nd International Conference on Bioinformatics and Bioengineering (BIBE)

Frontiers in Physics

Mitochondria are dynamic organelles that integrate bioenergetics, biosynthesis, and signaling in ... more Mitochondria are dynamic organelles that integrate bioenergetics, biosynthesis, and signaling in cells and regulate redox homeostasis, apoptotic pathways, and cell proliferation and differentiation. Depending on the environmental conditions, the mitochondrial morphology dynamically changes to match the energy demands. The mitochondrial dynamics is related to the initiation, migration, and invasion of diverse human cancers and thus affects cancer metastasis, metabolism, drug resistance, and cancer stem cell survival. We reviewed the current image-based analytical tools and machine-learning techniques for phenotyping mitochondrial morphology in different cancer cell lines from confocal microscopy images. We listed and applied pipelines and packages available in ImageJ/Fiji, CellProfiler, MATLAB, Java, and Python for the analysis of fluorescently labeled mitochondria in microscopy images and compared their performance, usability and applications. Furthermore, we discussed the potential...

Cells

Oxidative phosphorylation (OXPHOS) is an oxygen-dependent process that consumes catabolized nutri... more Oxidative phosphorylation (OXPHOS) is an oxygen-dependent process that consumes catabolized nutrients to produce adenosine triphosphate (ATP) to drive energy-dependent biological processes such as excitation-contraction coupling in cardiomyocytes. In addition to in vivo and in vitro experiments, in silico models are valuable for investigating the underlying mechanisms of OXPHOS and predicting its consequences in both physiological and pathological conditions. Here, we compare several prominent kinetic models of OXPHOS in cardiomyocytes. We examine how their mathematical expressions were derived, how their parameters were obtained, the conditions of their experimental counterparts, and the predictions they generated. We aim to explore the general landscape of energy production mechanisms in cardiomyocytes for future in silico models.

SSRN Electronic Journal, 2021

?lang=en [resumo] As duas primeiras décadas do século XXI consistem em um período fértil para ref... more ?lang=en [resumo] As duas primeiras décadas do século XXI consistem em um período fértil para refletirmos sobre a diversidade de representações e estéticas corporais no contexto da comunicação midiática de marcas plus size, sejam elas globais ou locais. Abordamos, neste artigo, os entrelaçamentos da moda com a biopolítica, com o objetivo de problematizar como marcas de moda plus size, ao visibilizarem o corpo gordo, evidenciam a constituição de biopolíticas bastante afeitas às lógicas de mercado-o que denominamos biopolíticas do consumo. Para tanto, analisamos a comunicação de duas marcas de moda plus size, Savage X Fenty e Rainha Nagô, a fim de identificar características das biopolíticas do consumo contemporâneas em contextos marcários distintos. Para fundamentar teoricamente nossas reflexões, mobilizamos autores que se dedicam aos estudos de Consumo, Comunicação, Moda e Biopolítica. Quanto aos resultados alcançados, as biopolíticas do consumo manifestam-se na moda plus size entre o controle e o empoderamento. [palavras-chave] Moda plus size. Biopolíticas do consumo. Corpo gordo.

Cellular bioenergetics and mitochondrial dynamics are crucial for the secretion of insulin by pan... more Cellular bioenergetics and mitochondrial dynamics are crucial for the secretion of insulin by pancreatic beta cells in response to elevated blood glucose concentrations. To obtain better insights into the interactions between energy production and mitochondrial fission/fusion dynamics, we combine live-cell mitochondria imaging with biophysical-based modeling and network analysis to elucidate the principle regulating mitochondrial morphology to match metabolic demand in pancreatic beta cells. A minimalistic differential equation-based model for beta cells was constructed to include glycolysis, oxidative phosphorylation, simple calcium dynamics, and graph-based fission/fusion dynamics controlled by ATP synthase flux and proton leak flux. The model revealed that mitochondrial fission occurs in response to hyperglycemia, starvation, ATP synthase inhibition, uncoupling, and diabetic condition, in which the rate of proton leak exceeds the rate of mitochondrial ATP synthesis. Under these m...

Nature Communications

Despite the clear association between myocardial injury, heart failure and depressed myocardial e... more Despite the clear association between myocardial injury, heart failure and depressed myocardial energetics, little is known about upstream signals responsible for remodeling myocardial metabolism after pathological stress. Here, we report increased mitochondrial calmodulin kinase II (CaMKII) activation and left ventricular dilation in mice one week after myocardial infarction (MI) surgery. By contrast, mice with genetic mitochondrial CaMKII inhibition are protected from left ventricular dilation and dysfunction after MI. Mice with myocardial and mitochondrial CaMKII overexpression (mtCaMKII) have severe dilated cardiomyopathy and decreased ATP that causes elevated cytoplasmic resting (diastolic) Ca2+ concentration and reduced mechanical performance. We map a metabolic pathway that rescues disease phenotypes in mtCaMKII mice, providing insights into physiological and pathological metabolic consequences of CaMKII signaling in mitochondria. Our findings suggest myocardial dilation, a d...

Biophysical journal, Feb 1, 2024

2022 IEEE 22nd International Conference on Bioinformatics and Bioengineering (BIBE)

Frontiers in Physics

Mitochondria are dynamic organelles that integrate bioenergetics, biosynthesis, and signaling in ... more Mitochondria are dynamic organelles that integrate bioenergetics, biosynthesis, and signaling in cells and regulate redox homeostasis, apoptotic pathways, and cell proliferation and differentiation. Depending on the environmental conditions, the mitochondrial morphology dynamically changes to match the energy demands. The mitochondrial dynamics is related to the initiation, migration, and invasion of diverse human cancers and thus affects cancer metastasis, metabolism, drug resistance, and cancer stem cell survival. We reviewed the current image-based analytical tools and machine-learning techniques for phenotyping mitochondrial morphology in different cancer cell lines from confocal microscopy images. We listed and applied pipelines and packages available in ImageJ/Fiji, CellProfiler, MATLAB, Java, and Python for the analysis of fluorescently labeled mitochondria in microscopy images and compared their performance, usability and applications. Furthermore, we discussed the potential...

Cells

Oxidative phosphorylation (OXPHOS) is an oxygen-dependent process that consumes catabolized nutri... more Oxidative phosphorylation (OXPHOS) is an oxygen-dependent process that consumes catabolized nutrients to produce adenosine triphosphate (ATP) to drive energy-dependent biological processes such as excitation-contraction coupling in cardiomyocytes. In addition to in vivo and in vitro experiments, in silico models are valuable for investigating the underlying mechanisms of OXPHOS and predicting its consequences in both physiological and pathological conditions. Here, we compare several prominent kinetic models of OXPHOS in cardiomyocytes. We examine how their mathematical expressions were derived, how their parameters were obtained, the conditions of their experimental counterparts, and the predictions they generated. We aim to explore the general landscape of energy production mechanisms in cardiomyocytes for future in silico models.

SSRN Electronic Journal, 2021

?lang=en [resumo] As duas primeiras décadas do século XXI consistem em um período fértil para ref... more ?lang=en [resumo] As duas primeiras décadas do século XXI consistem em um período fértil para refletirmos sobre a diversidade de representações e estéticas corporais no contexto da comunicação midiática de marcas plus size, sejam elas globais ou locais. Abordamos, neste artigo, os entrelaçamentos da moda com a biopolítica, com o objetivo de problematizar como marcas de moda plus size, ao visibilizarem o corpo gordo, evidenciam a constituição de biopolíticas bastante afeitas às lógicas de mercado-o que denominamos biopolíticas do consumo. Para tanto, analisamos a comunicação de duas marcas de moda plus size, Savage X Fenty e Rainha Nagô, a fim de identificar características das biopolíticas do consumo contemporâneas em contextos marcários distintos. Para fundamentar teoricamente nossas reflexões, mobilizamos autores que se dedicam aos estudos de Consumo, Comunicação, Moda e Biopolítica. Quanto aos resultados alcançados, as biopolíticas do consumo manifestam-se na moda plus size entre o controle e o empoderamento. [palavras-chave] Moda plus size. Biopolíticas do consumo. Corpo gordo.

Cellular bioenergetics and mitochondrial dynamics are crucial for the secretion of insulin by pan... more Cellular bioenergetics and mitochondrial dynamics are crucial for the secretion of insulin by pancreatic beta cells in response to elevated blood glucose concentrations. To obtain better insights into the interactions between energy production and mitochondrial fission/fusion dynamics, we combine live-cell mitochondria imaging with biophysical-based modeling and network analysis to elucidate the principle regulating mitochondrial morphology to match metabolic demand in pancreatic beta cells. A minimalistic differential equation-based model for beta cells was constructed to include glycolysis, oxidative phosphorylation, simple calcium dynamics, and graph-based fission/fusion dynamics controlled by ATP synthase flux and proton leak flux. The model revealed that mitochondrial fission occurs in response to hyperglycemia, starvation, ATP synthase inhibition, uncoupling, and diabetic condition, in which the rate of proton leak exceeds the rate of mitochondrial ATP synthesis. Under these m...

Nature Communications

Despite the clear association between myocardial injury, heart failure and depressed myocardial e... more Despite the clear association between myocardial injury, heart failure and depressed myocardial energetics, little is known about upstream signals responsible for remodeling myocardial metabolism after pathological stress. Here, we report increased mitochondrial calmodulin kinase II (CaMKII) activation and left ventricular dilation in mice one week after myocardial infarction (MI) surgery. By contrast, mice with genetic mitochondrial CaMKII inhibition are protected from left ventricular dilation and dysfunction after MI. Mice with myocardial and mitochondrial CaMKII overexpression (mtCaMKII) have severe dilated cardiomyopathy and decreased ATP that causes elevated cytoplasmic resting (diastolic) Ca2+ concentration and reduced mechanical performance. We map a metabolic pathway that rescues disease phenotypes in mtCaMKII mice, providing insights into physiological and pathological metabolic consequences of CaMKII signaling in mitochondria. Our findings suggest myocardial dilation, a d...