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Wendy Batten

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Research paper thumbnail of Isolation of Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes that lyse Reed-Sternberg cells: implications for immune-mediated therapy of EBV+ Hodgkin's disease

Blood, Mar 15, 1997

Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the mal... more Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the malignant Reedthe dominant responses to EBV, we examined if CTL clones Sternberg (R-S) cells. R-S cells express only a limited set of specific for these proteins could be isolated despite the preslatent EBV proteins, but only LMP1 and LMP2 can potentially ence of weak or nondetectable responses in polyclonal T-cell elicit a CD8 " cytotoxic T-lymphocyte (CTL) response. We lines. LMP-specific clones were generated from individuals have evaluated if either of these proteins could be used as either by cloning from the polyclonal EBV-reactive T-cell targets for specific adoptive T-cell therapy for EBV-positive lines or by direct stimulation of peripheral blood mononu-(EBV " ) HD. The success of this strategy requires that R-S clear cells (PBMC) with cells expressing LMP1 or LMP2 as cells are susceptible to lysis by CD8 " CTL, and that CTL spethe only EBV protein. Our ability to isolate CTL specific for cific for LMP1 and LMP2 can be detected and potentially LMP proteins from individuals with HD and the sensitivity amplified in HD patients. Antigen presentation and CTL senof R-S cells for CTL-mediated lysis suggest that the pursuit sitivity was evaluated with an in vitro maintained, phenotypof specific adoptive immunotherapy represents a viable ically representative R-S cell line, HDLM-2. The R-S cells were strategy for the subset of HD patients with EBV " tumors. able to process and present viral proteins, and to be effi-᭧ 1997 by The American Society of Hematology. ciently lysed by specific CTL in a Class I-restricted manner.

Research paper thumbnail of Isolation of Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes that lyse Reed-Sternberg cells: implications for immune-mediated therapy of EBV+ Hodgkin's disease

Blood, Mar 15, 1997

Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the mal... more Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the malignant Reedthe dominant responses to EBV, we examined if CTL clones Sternberg (R-S) cells. R-S cells express only a limited set of specific for these proteins could be isolated despite the preslatent EBV proteins, but only LMP1 and LMP2 can potentially ence of weak or nondetectable responses in polyclonal T-cell elicit a CD8 " cytotoxic T-lymphocyte (CTL) response. We lines. LMP-specific clones were generated from individuals have evaluated if either of these proteins could be used as either by cloning from the polyclonal EBV-reactive T-cell targets for specific adoptive T-cell therapy for EBV-positive lines or by direct stimulation of peripheral blood mononu-(EBV " ) HD. The success of this strategy requires that R-S clear cells (PBMC) with cells expressing LMP1 or LMP2 as cells are susceptible to lysis by CD8 " CTL, and that CTL spethe only EBV protein. Our ability to isolate CTL specific for cific for LMP1 and LMP2 can be detected and potentially LMP proteins from individuals with HD and the sensitivity amplified in HD patients. Antigen presentation and CTL senof R-S cells for CTL-mediated lysis suggest that the pursuit sitivity was evaluated with an in vitro maintained, phenotypof specific adoptive immunotherapy represents a viable ically representative R-S cell line, HDLM-2. The R-S cells were strategy for the subset of HD patients with EBV " tumors. able to process and present viral proteins, and to be effi-᭧ 1997 by The American Society of Hematology. ciently lysed by specific CTL in a Class I-restricted manner.

Research paper thumbnail of The gp130-stimulating designer cytokine hyper-IL-6 promotes the expansion of human hematopoietic progenitor cells capable to differentiate into functional dendritic cells

Experimental Hematology, 2000

Objective . Hyper-IL-6, a fusion protein of interleukin-6 and its specific receptor, together wit... more Objective . Hyper-IL-6, a fusion protein of interleukin-6 and its specific receptor, together with stem cell factor leads to the proliferation of primitive hematopoietic progenitor cells. Based on these findings, the current study examined whether hyper-IL-6 promotes the growth of precursor cells that can be further differentiated into dendritic cells in the presence of additional cytokines.

Research paper thumbnail of Dendritic Cells Lose Ability to Present Protein Antigen after Stimulating Antigen-Specific T Cell Responses, despite Upregulation of MHC Class II Expression

Immunobiology, 2000

Immature dendritic cells (DC) take up, process and present protein antigens; mature DC are specia... more Immature dendritic cells (DC) take up, process and present protein antigens; mature DC are specialized for stimulating primary T cell responses with increased expression of MHC class II and co-stimulatory molecules, but are incapable of processing and presenting soluble protein.

Research paper thumbnail of Isolation of Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes that lyse Reed-Sternberg cells: implications for immune-mediated therapy of EBV+ Hodgkin's disease

Blood, Mar 15, 1997

Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the mal... more Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the malignant Reedthe dominant responses to EBV, we examined if CTL clones Sternberg (R-S) cells. R-S cells express only a limited set of specific for these proteins could be isolated despite the preslatent EBV proteins, but only LMP1 and LMP2 can potentially ence of weak or nondetectable responses in polyclonal T-cell elicit a CD8 " cytotoxic T-lymphocyte (CTL) response. We lines. LMP-specific clones were generated from individuals have evaluated if either of these proteins could be used as either by cloning from the polyclonal EBV-reactive T-cell targets for specific adoptive T-cell therapy for EBV-positive lines or by direct stimulation of peripheral blood mononu-(EBV " ) HD. The success of this strategy requires that R-S clear cells (PBMC) with cells expressing LMP1 or LMP2 as cells are susceptible to lysis by CD8 " CTL, and that CTL spethe only EBV protein. Our ability to isolate CTL specific for cific for LMP1 and LMP2 can be detected and potentially LMP proteins from individuals with HD and the sensitivity amplified in HD patients. Antigen presentation and CTL senof R-S cells for CTL-mediated lysis suggest that the pursuit sitivity was evaluated with an in vitro maintained, phenotypof specific adoptive immunotherapy represents a viable ically representative R-S cell line, HDLM-2. The R-S cells were strategy for the subset of HD patients with EBV " tumors. able to process and present viral proteins, and to be effi-᭧ 1997 by The American Society of Hematology. ciently lysed by specific CTL in a Class I-restricted manner.

Research paper thumbnail of Isolation of Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes that lyse Reed-Sternberg cells: implications for immune-mediated therapy of EBV+ Hodgkin's disease

Blood, Mar 15, 1997

Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the mal... more Since CTL responses to LMP1 and LMP2 do not represent Epstein-Barr virus (EBV) genomes in the malignant Reedthe dominant responses to EBV, we examined if CTL clones Sternberg (R-S) cells. R-S cells express only a limited set of specific for these proteins could be isolated despite the preslatent EBV proteins, but only LMP1 and LMP2 can potentially ence of weak or nondetectable responses in polyclonal T-cell elicit a CD8 " cytotoxic T-lymphocyte (CTL) response. We lines. LMP-specific clones were generated from individuals have evaluated if either of these proteins could be used as either by cloning from the polyclonal EBV-reactive T-cell targets for specific adoptive T-cell therapy for EBV-positive lines or by direct stimulation of peripheral blood mononu-(EBV " ) HD. The success of this strategy requires that R-S clear cells (PBMC) with cells expressing LMP1 or LMP2 as cells are susceptible to lysis by CD8 " CTL, and that CTL spethe only EBV protein. Our ability to isolate CTL specific for cific for LMP1 and LMP2 can be detected and potentially LMP proteins from individuals with HD and the sensitivity amplified in HD patients. Antigen presentation and CTL senof R-S cells for CTL-mediated lysis suggest that the pursuit sitivity was evaluated with an in vitro maintained, phenotypof specific adoptive immunotherapy represents a viable ically representative R-S cell line, HDLM-2. The R-S cells were strategy for the subset of HD patients with EBV " tumors. able to process and present viral proteins, and to be effi-᭧ 1997 by The American Society of Hematology. ciently lysed by specific CTL in a Class I-restricted manner.

Research paper thumbnail of The gp130-stimulating designer cytokine hyper-IL-6 promotes the expansion of human hematopoietic progenitor cells capable to differentiate into functional dendritic cells

Experimental Hematology, 2000

Objective . Hyper-IL-6, a fusion protein of interleukin-6 and its specific receptor, together wit... more Objective . Hyper-IL-6, a fusion protein of interleukin-6 and its specific receptor, together with stem cell factor leads to the proliferation of primitive hematopoietic progenitor cells. Based on these findings, the current study examined whether hyper-IL-6 promotes the growth of precursor cells that can be further differentiated into dendritic cells in the presence of additional cytokines.

Research paper thumbnail of Dendritic Cells Lose Ability to Present Protein Antigen after Stimulating Antigen-Specific T Cell Responses, despite Upregulation of MHC Class II Expression

Immunobiology, 2000

Immature dendritic cells (DC) take up, process and present protein antigens; mature DC are specia... more Immature dendritic cells (DC) take up, process and present protein antigens; mature DC are specialized for stimulating primary T cell responses with increased expression of MHC class II and co-stimulatory molecules, but are incapable of processing and presenting soluble protein.

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