Werner Voss - Academia.edu (original) (raw)

Papers by Werner Voss

Journal of The Mechanical Behavior of Biomedical Materials, Oct 1, 2021

We report a custom-made technique to synthesize process-convenient skin scaffolds by tuning the m... more We report a custom-made technique to synthesize process-convenient skin scaffolds by tuning the mechanical properties of hydrogels based on a few naturally occurring polysaccharides to match the rheological properties of previously established benchmarks, i.e., the ex vivo native human skins. We studied the mechanical parameters using oscillatory shear rheology. At small strain amplitudes, the intrinsic elastic modulus showed an almost linear dependence in the middle and a changing rate profile at the two ends with concentration of the principal hydrogel component variant, i.e., kappa (κ)-carrageenan. At large strain amplitudes, the hydrogels demonstrated intercycle strain-softening behavior, the onset of which was directly proportional to the κ-carrageenan concentration. We observed a concentration match for the intrinsic elastic modulus of the benchmark within this sigmoidal curve fit. Contextually, we need to explore other potent polymeric hydrogel systems to achieve mechanical affinity in terms of multiple rheological parameters derived from both strain amplitude and angular frequency sweeps. Additionally, we carried out diffusion experiments to study caffeine permeation attributes. The hydrogels show improved barrier features with increasing κ-carrageenan concentration. In terms of the penetration flux and total cumulative amount of permeated caffeine, this enhanced mechanical adherence demonstrates comparable penetration features with the commercial 3D skin model.

Aktuelle Dermatologie, Oct 5, 2015

Consumers have become aware of increasing risk of skin cancer and premature ageing due to unprote... more Consumers have become aware of increasing risk of skin cancer and premature ageing due to unprotected sun exposure; sun protection has been widely advised for many years. Sunscreen is the largest sector of the sun care market and higher SPFs (20+) are increasing in demand. Such products always refer to the “healthy tanned” body ideal. But we should be aware of the variety of effects due to UV-irradiation, which are not always beneficial. UVirradiation in low doses supports the immune system. In increasing doses it influences the immune system by suppressing the antigenic presenting cells in the skin. The Langerhans cells, which lie in the suprabasal layer of the skin, play a central role in recognising foreign antigens and in activating other effector cells. After UV-exposition a loss of function or even Langerhans cell apoptosis can follow. As it causes inflammation of the cornea, degeneration of the retina and cataracts, UV-irradiation can also lead to blindness. The skin diseases...

Data Revues 07533322 Unassign S0753332212000224, Jun 3, 2012

Munster: Dermatest GmbH [available from Dermatest]

Surface roughness is an important criterion for assessing the health status of the skin. Changes ... more Surface roughness is an important criterion for assessing the health status of the skin. Changes in roughness occur, among other things, in the case of congenital keratinization disturbances, environment-and job-related skin irritations (toxical and allergic eczema), ...

Biomedicine & Pharmacotherapy, 2012

Psoriasis and atopic dermatitis (AD) are challenging to treat due to the absence of suitable moni... more Psoriasis and atopic dermatitis (AD) are challenging to treat due to the absence of suitable monitoring procedure and their recurrences. Alteration of skin hydrophilic biomarkers (SHB) and structural elements occur in both disorders and may possess a distinct profile for each clinical condition. To quantify skin cytokines and antioxidants non-invasively in psoriatic and in AD patients and to evaluate skin auto-fluorescence in psoriatic patients. A skin wash sampling technique was utilized to detect the expression of SHB on psoriatic and AD patients and healthy controls. Inflammatory cytokine (TNFα, IL-1α and IL-6) levels, total antioxidant scavenging capacity and uric acid content were estimated. Additionally, measurement of the fluorescent emission spectra of tryptophan moieties, collagen cross-links and elastin cross-links were performed on psoriatic patients and healthy controls. Our findings demonstrate significant alterations of the SHB levels among psoriasis, AD and healthy skin. Differences were also observed between lesional and non-lesional areas in patients with psoriasis and AD. Ultra-structural changes were found in psoriatic patients both in lesional and non-lesional areas. Employing non-invasive measurements of skin wash sampling and skin auto-fluorescence might serve as complementary analysis for improved diagnosis and treatment of psoriasis and AD. Furthermore, they may serve as an additional monitoring tool for various diseases, in which skin dysfunction is involved.

Heliyon, 2021

Five different galenics were analyzed and compared concerning tissue breathability and gas exchan... more Five different galenics were analyzed and compared concerning tissue breathability and gas exchange with the environment after an application period of 6 h on pig ear skin. Aim was to find the most suitable galenics for efficient moist treatment for everyday injuries (abrasions, lacerations and cuts) without influencing the transepidermal water loss. Methods: A quantity of 0.1 g of the different test preparations was applied once topically to an area of 2 cm 2. The analysis of the breathability was performed by TEWL (transepidermal water loss) measurements in the first hour after product application. The moisture retention effect was assessed by corneometry in the first 5 h after product application. Results: The hydrogel preparations showed a higher breathability in contrast to a semi-occlusive ointment and petrolatum. The same applies to the moisture penetration of the skin. Here, all hydrogel formulations showed the highest tissue hydration. After 3 h an additional increase in moisture was observed for the areas treated with Tyrosur® CareExpert Wound Gel and the ointment. Conclusion: In contrast to petrolatum and the semi-occlusive ointment, treatment with the hydrogels led to a preservation of the breathability and good moistening of the tissue, which is due to the galenics of the gels consisting of water, carbomer and propylene glycol. The increase in moisture after 3 h in areas treated with Tyrosur® CareExpert Wound Gel and the semi-occlusive ointment indicates a sustained moisturizing effect mediated by dexpanthenol.

Clinical, Cosmetic and Investigational Dermatology, 2013

Journal of Cosmetics, Dermatological Sciences and Applications

Background: Progressing androgenetic alopecia (AGA), in both sexes, can result in severe distress... more Background: Progressing androgenetic alopecia (AGA), in both sexes, can result in severe distress. Treatments with the capacity to slow down the progression of AGA, or even to bring it to a halt, and at the same time don't come with side effects are consequently highly sought for. Therefore this study investigates the effect of an over-the-counter nutritional supplement and a similarly formulated topical hair lotion on the progression of AGA. Methods: Seventy-nine healthy study participants of both sexes, who were diagnosed with AGA were divided into 4 study groups. The subjects of the first group were treated with the nutritional supplement, the subjects of the second group with the topical hair lotion, the subjects of the third group with both products, and the subjects of the fourth group served as a no-treatment control. At the beginning and at the end of this nine-month study, the participants were evaluated for their hair loss status. They also answered a questionnaire for self-assessment. A part of the subjects from each study group were further analysed by phototrichography, in order to measure the number of anagen and telogen hairs. Results: It turned out that the supplement, the lotion as well as the treatment with both products not only lead to a reduction in hair loss but also to an increased anagen to telogen hair ratio, whereas no such effects could be measured for the control group. Conclusion: The results show that a systemic delivery via a nutritional supplement, as well as a follicular delivery via a topically applied lotion, both resulted in a reduced hair loss rate as well as in an increased anagen to telogen hair ratio. This demonstrates that the tested formulation is effectively slowing down the progression of AGA.

Nutrients

The purpose of this randomized, placebo-controlled, blind study was to investigate the effects of... more The purpose of this randomized, placebo-controlled, blind study was to investigate the effects of the drinkable nutraceutical ELASTEN® (QUIRIS Healthcare, Gütersloh, Germany) on skin aging and skin health. Drinking ampoules provides a blend of 2.5 g of collagen peptides, acerola fruit extract, vitamin C, zinc, biotin, and a native vitamin E complex. This controlled interventional trial was performed on 72 healthy women aged 35 years or older. They received either the food supplement (n = 36) or a placebo (n = 36) for twelve weeks. A skin assessment was carried out and based on objective validated methods, including corneometry (skin hydration), cutometry (elasticity), the use of silicon skin replicas with optical 3D phase-shift rapid in-vivo measurements (PRIMOS) (roughness), and skin sonography (density). The verum group was followed for an additional four weeks (without intake of the test product) to evaluate the sustainability of the changes induced by the intake of the test prod...

Journal of Evidence-Based Complementary & Alternative Medicine

Intake of oral supplements with the aim of a cutaneous antiaging effect are increasingly common. ... more Intake of oral supplements with the aim of a cutaneous antiaging effect are increasingly common. Hyaluronic acid (HA) is a promising candidate, as it is the key factor for preserving tissue hydration. In our practice study, we evaluated the effect of an oral HA preparation diluted in a cascade-fermented organic whole food concentrate supplemented with biotin, vitamin C, copper, and zinc (Regulatpro Hyaluron) on skin moisture content, elasticity, skin roughness, and wrinkle depths. Twenty female subjects with healthy skin in the age group of 45 to 60 years took the product once daily for 40 days. Different skin parameters were objectively assessed before the first intake, after 20 and after 40 days. Intake of the HA solution led to a significant increase in skin elasticity, skin hydration, and to a significant decrease in skin roughness and wrinkle depths. The supplement was well tolerated; no side effects were noted throughout the study.

Journal of the Mechanical Behavior of Biomedical Materials

Operative Dermatologie, 1979

Pharmazeutische Zeitung, 2005

Dermatological Reports on Cosmetics: Possibilites and Pitfalls Werner Voss, M.D. Ilsabe Bunge, Ph... more Dermatological Reports on Cosmetics: Possibilites and Pitfalls Werner Voss, M.D. Ilsabe Bunge, Ph.D., M.D. Dermatest Medical Research Company www.dermatest.de Introduction The cosmetic industry is booming and so are the claims concerning the degree of consumer compatibility and the effectiveness of cosmetic products. A cosmetic today has to fulfill many claims and demands and is not only responsible for decorative effect. Most important of all, it has to be well-tolerated by the skin. Not only have product demands changed, but the average skin condition has changed as well. Skin conditions have gone to extremes in the general population. There are growing groups with dry skin, sensitive skin and seborrhoic skin that have to be considered when formulating skin care products, for problematic skin conditions must be compatible with cosmetic support. Toxicity and Regulation Traditionally cosmetics have been principally concerned with beautifying and with decoration, rather than with fun...

Journal of Cosmetics, Dermatological Sciences and Applications, 2014

Background: Exposure to solar UV rays has deleterious effects on skin appearance through physiolo... more Background: Exposure to solar UV rays has deleterious effects on skin appearance through physiological and structural alterations that eventually lead to skin photo-damage. Aims: To test the photo-protective effect of a novel preparation, Dead Sea Osmoter Concentrate (DSOC), containing high concentration of Dead Sea water. Methods: Human skin organ culture was used as a model to assess the biological effects of UVB irradiation and the protective effect of topical application of DSOC preparation. Skin pieces were analyzed for mitochondrial activity by Alamar blue (resazurin) assay, for apoptosis by caspase 3 assay, for detoxification by proteasome 20S sub-unit activity and for skin hydration by aquaporin 3 (AQP3) membrane channels expression. Human subjects were tested to evaluate the effect of DSOC Serum, a topical formula for facial skin, on skin hydration by a corneometer. Results: UVB irradiation induced cell apoptosis in the epidermis of skin organ cultures and decreased their proteasome activity and AQP3 expression. Topical application of DSOC significantly attenuated all these effects. In human subjects, an elevation in the intense skin moisture, which was higher relatively to subjects' regular moisturizer, was observed. Moreover, DSOC Serum boosted the hydration effect together with regular moisturizer. Conclusions: The results clearly demonstrate that DSOC preparation can significantly improve the skin capability in coping with UVB rays stress in different cellular functions such as anti-apoptotic properties as well as detoxification and hydration recovery that can attenuate biological effects of skin photo-damage. Topical application of DSOC Serum had contributed to skin appearance by a strong hydration impact, also as a booster.

International journal of cosmetic science, 1997

The skin smoothing effects of three different liquid gels were compared on 20 mature women. Treat... more The skin smoothing effects of three different liquid gels were compared on 20 mature women. Treatment applications were performed twice a day over a period of 4 weeks, and the skin roughness parameter (Rz) of all test participants was determined at the beginning and at the end of the study using a computer-aided laser profilometry, in accordance with DIN 4768 ff. At the end of the application period, the liquid gel with 1% of a Dead Sea mineral solution had an average skin roughness parameter reduction of 40.7%. The liquid gel without mineral additives showed an average reduction in skin roughness of 27.8%. The control gel without anti-wrinkle agents or the additives showed an average reduction of only 10.4%.

Journal of Cosmetic Dermatology, 2012

Background Skin appearance is badly affected when exposed to solar UV rays, which encourage physi... more Background Skin appearance is badly affected when exposed to solar UV rays, which encourage physiological and structural cutaneous alterations that eventually lead to skin photo-damage. Aims To test the capability of two facial preparations, extreme day cream (EXD) and extreme night treatment (EXN), containing a unique complex of Dead Sea water and three Himalayan extracts, to antagonize biological effects induced by photo-damage. Methods Pieces of organ cultures of human skin were used as a model to assess the biological effects of UVB irradiation and the protective effect of topical application of two Extreme preparations. Skin pieces were analyzed for mitochondrial activity by MTT assay, for apoptosis by caspase 3 assay, and for cytokine secretion by solid phase ELISA. Human subjects were tested to evaluate the effect of Extreme preparations on skin wrinkle depth using PRIMOS and skin hydration by a corneometer. Results UVB irradiation induced cell apoptosis in the epidermis of skin organ cultures and increased their pro-inflammatory cytokine, tumor necrosis a (TNFa) secretion. Topical applications of both preparations significantly attenuated all these effects. Furthermore, in human subjects, a reduction in wrinkle depth and an elevation in the intense skin moisture were observed. Conclusions The observations clearly show that EXD and EXN preparations have protective anti-apoptotic and anti-inflammatory properties that can attenuate biological effects of skin photo-damage. Topical application of the preparations improves skin appearance by reducing its wrinkles depth and increasing its moisturizing impact.