Wieslawa Bylka - Academia.edu (original) (raw)
Papers by Wieslawa Bylka
Medical Science Monitor, 2012
Background: To date, Kaposi sarcoma has not been mentioned among the adverse effects of triptolid... more Background: To date, Kaposi sarcoma has not been mentioned among the adverse effects of triptolide/tripdiolide, ethyl acetate extracts or polyglycosides of the Chinese herbal remedy Tripterygium wilfordii Hook F.
702-711The ethyl ether extract of A. vulgaris inhibited in vitro microsomal lipid peroxidation (I... more 702-711The ethyl ether extract of A. vulgaris inhibited in vitro microsomal lipid peroxidation (IC50 58.8 µg/ml) and showed moderate ability to scavenge superoxide radicals and to chelate iron ions. The extract (100 mg/kg body weight, po) decreased uninduced and enzymatic microsomal lipid peroxidation in the liver of male rats pretreated with CCl4 (1 ml/kg body weight) by 27 and 40%, respectively. Activity of antioxidant and related enzymes (catalase and glucose-6-phosphate dehydrogenase) inhibited by CCl4 was significantly restored after administration of the extract. The extract itself significantly enhanced superoxide dismutase activity. There was no effect of the extract on hepatic glutathione level and cytochrome P450 content, both were decreased by CCl4. Neither CCl4 nor the tested extract affected activities of NADPH-cytochrome P450 reductase and two monooxygenases, aniline hydroxylase and aminopyrine n-demethylase. It can be concluded that the protective effect of the A. vul...
Postępy Fitoterapii, May 25, 2020
Postępy Fitoterapii, 2020
Licorice has been used in medicine from ancient times. Licorice preparations are applied in infec... more Licorice has been used in medicine from ancient times. Licorice preparations are applied in infectious diseases of the respiratory tract, peptic and duodenal ulcers, as well as in skin diseases and cosmetology. Sweet taste of root has appointed licorice role of corrigens. Licorice root contains active compounds with different activities: saponins, flavonoids, coumarins and essential oil. In vitro and in vivo studies have shown that extracts of licorice and its active compounds have different properties, such as expectorant, antitussive, antibacterial, anti-inflammatory and hepatoprotective.
Postępy Fitoterapii, May 25, 2020
Postępy Fitoterapii, May 25, 2020
Postępy Fitoterapii, 2019
<jats:p>Introduction. Diabetes is characterized by chronic hyperglycaemia, resulting from a... more <jats:p>Introduction. Diabetes is characterized by chronic hyperglycaemia, resulting from a defect in insulin secretion or abnormal functioning of this hormone. The consequence of the lack of insulin is the disturbed metabolism of carbohydrates, proteins and fats. Chronic hyperglycemia causes damage and dysfunction of many organs. One of the ways to support the treatment of diabetes is the use of plant preparations. Aim. The aim of the study was to examine and compare the biological (α-glucosidase and antioxidative) activity and the content of polyphenols and flavonoids in selected plants: Anserinae herba, Alchemillae herba and Fragariae folium, included in herbal tea blends used in diabetes. Material and methods. The content of polyphenols was determined by applying the Folin-Ciocalteu reagent and the flavonoid content by the AlCl3 method. The antioxidant properties were tested using the reagent method DPPH and CUPRAC, while the inhibitory activity of α-glucosidase by measuring the absorbance of p-nitrophenol from PNPG released by hydrolysis of sugars by the enzyme. Results. High total polyphenols content in the tested plant extracts was found, amounting to 121.30; 98.30; 71.66 mg gallic acid equivalent/g for Alchemillae herba, Fragariae folium and Anserinae herba, respectively, and high flavonoid content (17.56, 12.25, 15.48 mg quercetin equivalent/g, respectively). Antioxidant activity for extracts with a concentration of 0.312 mg/ml was from 46.22 to 79.71% (in the analysis with DPPH radical), while the absorbance value of these tests determined by the CUPRAC method was A = 0.49-1.01. The effect was weaker than vitamin C. The results indicated a strong inhibitory ability of α-glucosidase, which was > 98% for all tested extracts at the concentration of 0.1 mg/ml, and was many times higher than the standard acarbose analyzed at the above concentration, and comparable with the acrabose activity at a concentration of 10 mg/ml. Conclusions. The inhibitory α-glucosidase and antioxidant activity indicates the existence of this mechanism included in analyzed plant extracts, as ingredients herbal tea blends of used in diabetes.</jats:p>
Postępy Fitoterapii, 2020
Essential oils are mixtures of numerous chemical compounds, especially terpenes also phenylpropan... more Essential oils are mixtures of numerous chemical compounds, especially terpenes also phenylpropane derivatives. Essential oils components are characterized by different biological activity. The paper presents the most recent literature data concerning the activity of the compounds, which are common components oils: linalool, pulegone, santalol, spathulenol, α-terpineol and β-jonone.
Postępy Fitoterapii, 2020
Introduction. Licorice root is used in folk medicine to treat cough, in addition to gastrointesti... more Introduction. Licorice root is used in folk medicine to treat cough, in addition to gastrointestinal inflammation and as a hepatoprotective and anti-cancer, antiallergic agent, locally for eczema, psoriasis, inflammation and skin pigmentation. The main active compounds are triterpene saponins and numerous flavonoids of various structures. Aim. The purpose of our research was to analyze the components of volatile oil obtained from commercial licorice root. Material and methods. The oil was obtained by hydrodistillation in a Clevenger apparatus and its composition was analyzed by GC-MS. Results. Among 47 compounds identified, mainly (47.42%), terpenes were present, in the dominant amount anethole (15.44%) and carvone (15.16%), in a smaller amount were present, among others: limonene, carvacrol, linalool, p-cymene, menthol, mentone, p-cymenol, palmitic acid, α-terpineol, geranyl tyglate. Conclusions. The identified compounds may be co-responsible for the biological activity of licorice...
Postępy Fitoterapii, 2020
Licorice has been used in medicine for ancient times. Licorice root contains active compounds wit... more Licorice has been used in medicine for ancient times. Licorice root contains active compounds with different activities: saponins, flavonoids, coumarins and essential oil. It shows diverse biological properties. This article presents current achievements with clinical trials and creates new possibilities to use licorice in therapy. It has been proved effectiveness of licorice preparations in infectious diseases of respiratory tract, aphthous stomatitis, in dermatoses and also in skin care. The conducted researches on licorice preparations, especially DGL, present on the pharmaceutical market, which are recommended in gastrointestinal ulceration, have not proved their anti-ulcerative effect. The results of some clinical trials may, however, raise doubts due to the small groups and sometimes the lack of randomization or standardization of the studied preparation. Due to its sweet taste, it is used as a corrigens.
Phytochemistry Letters, 2019
The aim of this study was to evaluate the utility of a hydroalcoholic extract of Hippocastani flo... more The aim of this study was to evaluate the utility of a hydroalcoholic extract of Hippocastani flos (HF) in wound healing, especially in regards to cell viability, proliferation, reactive oxygen species (ROS) generation and proinflammatory cytokine production using an endothelial cell line [human umbilical vein endothelial cell (HUVEC) line EAhy926]. Antibacterial and anti-hyaluronidase activity was also evaluated. The phytochemical profile was determined using an the UPLC-MS. The UPLC-MS analysis confirmed the presence of nine flavonoids, mainly kaempferol and quercetin derivatives. Specifically, the HF extract contained a high content of polyphenols (88.84 ± 4.13 mg chlorogenic acid equivalents/g dw), including flavonoids (15.68 ± 0.04 mg/quercetin equivalents/g dw). Antioxidant properties were demonstrated using FRAP assays (IC 0.5 = 159.82 μg/mL). The HF extract exhibited a level of anti-hyaluronidase activity (IC 50 = 0.86 mg/mL) similar to the positive control kaempferol (IC 50 = 0.78 mg/mL). The antimicrobial properties of HF were shown against Staphylococcus aureus (MIC, MBC = 12.5 mg/mL). After exposing the endothelial cells to HF extract in vitro, it did not observed any change in the cell viability, proliferation, wound healing or proinflammatory cytokine production, but there was a dose dependent-reduction in ROS generation. In conclusion, HF extract exhibits anti-hyaluronidase activity, antimicrobial properties, and reduces ROS production. These observations may partly justify the use of HF in supporting wound healing.
Alnus glutinosa and A. incana (Betulaceae), are a small to medium size tree, native to the Northe... more Alnus glutinosa and A. incana (Betulaceae), are a small to medium size tree, native to the Northern Hemisphere. The leaves of this species are used in the Republic of Belarus as a source of antioxidants. The aim of this work was investigation of antioxidant activities and total phenolics content in various extracts obtained using water, mixture water with ethanol (from 9:1 to 2:8), and ethanol from A. glutinosa and A. incana leaves. Phenolics content was determined by method with Folin-Ciocalteu reagent and calculated on ellagic acid. The antioxidant activities were measured utilising 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical scavenging test. The highest phenolics concentration was observed in the extracts prepared by extraction with the mixture of water:ethanol from 7:3 to 3:7 and ranged from 17.82% to 18.96% for A. glutinosa and from 10.82% to 12.55% for A. incana. This extracts exhibited the highest free radical scavenging activity ranging from 49.21% to 49.42% and from 41.28% to 41.67% for A. glutinosa and A. incana respectively, comparable to the activity of quercetin. Therefore the mixture of water:ethanol from 7:3 to 3:7 should be used for preparing extracts from this species for medicinal purposes. Results also indicated the existence of a high correlation between antioxidant activity and total phenolics content.
Acta Physiologiae Plantarum, 2009
The gas chromatography-mass spectrometry (GC-MS) analysis of essential oil obtained by hydrodisti... more The gas chromatography-mass spectrometry (GC-MS) analysis of essential oil obtained by hydrodistillation from the flower of Taraxacum officinale L. revealed the presence of 25 compounds with 1,3-dimethylbenzene, 1,2-dimethylbenzene, 1-ethyl-3-methylbenzene, heneicosane and tricosane as the main components.
Acta Physiologiae Plantarum, 2001
Two flavonoid sulphates, i.e. quercetin 3-O-sulphate-7-O-aarabinopyranoside and kaempferol 3-O-su... more Two flavonoid sulphates, i.e. quercetin 3-O-sulphate-7-O-aarabinopyranoside and kaempferol 3-O-sulphate-7-O-a-arabinopyranoside, were isolated from leaves ofAtriplex hortensis L. The structures of these compounds were established by UV, 1H and 13C NMR, 2D NMR and MS spectra. The compounds were isolated for the first time from plant material.
Acta Chromatographica, 2008
Densitometric HPTLC and HPLC have been used for quantification of p-coumaric and protocatechuic a... more Densitometric HPTLC and HPLC have been used for quantification of p-coumaric and protocatechuic acids in an ethereal fraction from a methanolic extract of Aquilegia vulgaris L. HPLC analysis was performed on an RP-18 column with methanolwater-formic acid 25:75:0.5 (v/v) as mobile phase. Thin layer chromatography was performed on Si60 F 254 HPTLC plates with mixtures of heptane, dichloromethane, diisopropyl ether, formic acid, and water as mobile phases. Satisfactory separation of the phenolic acids was achieved by use of the multiple gradient development technique. The quantities of p-coumaric and protocatechuic acids determined by HPLC were 0.374 and 2.283 mg g −1 dry plant material, respectively; HPTLC results were somewhat higher-0.396 and 2.584 mg g −1 , respectively. The precision of both methods, expressed as relative standard deviation, was satisfactory. The methods are useful for quality control of Aquilegia vulgaris extracts.
Acta Biologica Cracoviensia Series Botanica, 2010
Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the an... more Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the antioxidant activity of three flavonoids with different polarity: 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside) and I 3' II 8 biapigenine (amentoflavone), with nonpolar all-trans β-carotene used as standard carotenoid molecule. The ABTS [2,2'azino-bis(3-ethylbenzthiazoline-6-sulphonic acid] cation radical decolorization assay was modified as follows: (1) measurements extended up to 8 days after preparation, (2) method adapted for flavonoids with different polarity and β-carotene, (3) concentrations in the 0.01-10 μM range of both trolox and antioxidants in order to use the same experimental conditions for both this technique and EPR measurement. K Ke ey y w wo or rd ds s: : 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside), I 3' II 8 biapigenine (amentoflavone), all-trans β-carotene, ABTS cation radical, TEAC, EPR technique, free radicals, oxidation.
Medical Science Monitor, 2012
Background: To date, Kaposi sarcoma has not been mentioned among the adverse effects of triptolid... more Background: To date, Kaposi sarcoma has not been mentioned among the adverse effects of triptolide/tripdiolide, ethyl acetate extracts or polyglycosides of the Chinese herbal remedy Tripterygium wilfordii Hook F.
702-711The ethyl ether extract of A. vulgaris inhibited in vitro microsomal lipid peroxidation (I... more 702-711The ethyl ether extract of A. vulgaris inhibited in vitro microsomal lipid peroxidation (IC50 58.8 µg/ml) and showed moderate ability to scavenge superoxide radicals and to chelate iron ions. The extract (100 mg/kg body weight, po) decreased uninduced and enzymatic microsomal lipid peroxidation in the liver of male rats pretreated with CCl4 (1 ml/kg body weight) by 27 and 40%, respectively. Activity of antioxidant and related enzymes (catalase and glucose-6-phosphate dehydrogenase) inhibited by CCl4 was significantly restored after administration of the extract. The extract itself significantly enhanced superoxide dismutase activity. There was no effect of the extract on hepatic glutathione level and cytochrome P450 content, both were decreased by CCl4. Neither CCl4 nor the tested extract affected activities of NADPH-cytochrome P450 reductase and two monooxygenases, aniline hydroxylase and aminopyrine n-demethylase. It can be concluded that the protective effect of the A. vul...
Postępy Fitoterapii, May 25, 2020
Postępy Fitoterapii, 2020
Licorice has been used in medicine from ancient times. Licorice preparations are applied in infec... more Licorice has been used in medicine from ancient times. Licorice preparations are applied in infectious diseases of the respiratory tract, peptic and duodenal ulcers, as well as in skin diseases and cosmetology. Sweet taste of root has appointed licorice role of corrigens. Licorice root contains active compounds with different activities: saponins, flavonoids, coumarins and essential oil. In vitro and in vivo studies have shown that extracts of licorice and its active compounds have different properties, such as expectorant, antitussive, antibacterial, anti-inflammatory and hepatoprotective.
Postępy Fitoterapii, May 25, 2020
Postępy Fitoterapii, May 25, 2020
Postępy Fitoterapii, 2019
<jats:p>Introduction. Diabetes is characterized by chronic hyperglycaemia, resulting from a... more <jats:p>Introduction. Diabetes is characterized by chronic hyperglycaemia, resulting from a defect in insulin secretion or abnormal functioning of this hormone. The consequence of the lack of insulin is the disturbed metabolism of carbohydrates, proteins and fats. Chronic hyperglycemia causes damage and dysfunction of many organs. One of the ways to support the treatment of diabetes is the use of plant preparations. Aim. The aim of the study was to examine and compare the biological (α-glucosidase and antioxidative) activity and the content of polyphenols and flavonoids in selected plants: Anserinae herba, Alchemillae herba and Fragariae folium, included in herbal tea blends used in diabetes. Material and methods. The content of polyphenols was determined by applying the Folin-Ciocalteu reagent and the flavonoid content by the AlCl3 method. The antioxidant properties were tested using the reagent method DPPH and CUPRAC, while the inhibitory activity of α-glucosidase by measuring the absorbance of p-nitrophenol from PNPG released by hydrolysis of sugars by the enzyme. Results. High total polyphenols content in the tested plant extracts was found, amounting to 121.30; 98.30; 71.66 mg gallic acid equivalent/g for Alchemillae herba, Fragariae folium and Anserinae herba, respectively, and high flavonoid content (17.56, 12.25, 15.48 mg quercetin equivalent/g, respectively). Antioxidant activity for extracts with a concentration of 0.312 mg/ml was from 46.22 to 79.71% (in the analysis with DPPH radical), while the absorbance value of these tests determined by the CUPRAC method was A = 0.49-1.01. The effect was weaker than vitamin C. The results indicated a strong inhibitory ability of α-glucosidase, which was > 98% for all tested extracts at the concentration of 0.1 mg/ml, and was many times higher than the standard acarbose analyzed at the above concentration, and comparable with the acrabose activity at a concentration of 10 mg/ml. Conclusions. The inhibitory α-glucosidase and antioxidant activity indicates the existence of this mechanism included in analyzed plant extracts, as ingredients herbal tea blends of used in diabetes.</jats:p>
Postępy Fitoterapii, 2020
Essential oils are mixtures of numerous chemical compounds, especially terpenes also phenylpropan... more Essential oils are mixtures of numerous chemical compounds, especially terpenes also phenylpropane derivatives. Essential oils components are characterized by different biological activity. The paper presents the most recent literature data concerning the activity of the compounds, which are common components oils: linalool, pulegone, santalol, spathulenol, α-terpineol and β-jonone.
Postępy Fitoterapii, 2020
Introduction. Licorice root is used in folk medicine to treat cough, in addition to gastrointesti... more Introduction. Licorice root is used in folk medicine to treat cough, in addition to gastrointestinal inflammation and as a hepatoprotective and anti-cancer, antiallergic agent, locally for eczema, psoriasis, inflammation and skin pigmentation. The main active compounds are triterpene saponins and numerous flavonoids of various structures. Aim. The purpose of our research was to analyze the components of volatile oil obtained from commercial licorice root. Material and methods. The oil was obtained by hydrodistillation in a Clevenger apparatus and its composition was analyzed by GC-MS. Results. Among 47 compounds identified, mainly (47.42%), terpenes were present, in the dominant amount anethole (15.44%) and carvone (15.16%), in a smaller amount were present, among others: limonene, carvacrol, linalool, p-cymene, menthol, mentone, p-cymenol, palmitic acid, α-terpineol, geranyl tyglate. Conclusions. The identified compounds may be co-responsible for the biological activity of licorice...
Postępy Fitoterapii, 2020
Licorice has been used in medicine for ancient times. Licorice root contains active compounds wit... more Licorice has been used in medicine for ancient times. Licorice root contains active compounds with different activities: saponins, flavonoids, coumarins and essential oil. It shows diverse biological properties. This article presents current achievements with clinical trials and creates new possibilities to use licorice in therapy. It has been proved effectiveness of licorice preparations in infectious diseases of respiratory tract, aphthous stomatitis, in dermatoses and also in skin care. The conducted researches on licorice preparations, especially DGL, present on the pharmaceutical market, which are recommended in gastrointestinal ulceration, have not proved their anti-ulcerative effect. The results of some clinical trials may, however, raise doubts due to the small groups and sometimes the lack of randomization or standardization of the studied preparation. Due to its sweet taste, it is used as a corrigens.
Phytochemistry Letters, 2019
The aim of this study was to evaluate the utility of a hydroalcoholic extract of Hippocastani flo... more The aim of this study was to evaluate the utility of a hydroalcoholic extract of Hippocastani flos (HF) in wound healing, especially in regards to cell viability, proliferation, reactive oxygen species (ROS) generation and proinflammatory cytokine production using an endothelial cell line [human umbilical vein endothelial cell (HUVEC) line EAhy926]. Antibacterial and anti-hyaluronidase activity was also evaluated. The phytochemical profile was determined using an the UPLC-MS. The UPLC-MS analysis confirmed the presence of nine flavonoids, mainly kaempferol and quercetin derivatives. Specifically, the HF extract contained a high content of polyphenols (88.84 ± 4.13 mg chlorogenic acid equivalents/g dw), including flavonoids (15.68 ± 0.04 mg/quercetin equivalents/g dw). Antioxidant properties were demonstrated using FRAP assays (IC 0.5 = 159.82 μg/mL). The HF extract exhibited a level of anti-hyaluronidase activity (IC 50 = 0.86 mg/mL) similar to the positive control kaempferol (IC 50 = 0.78 mg/mL). The antimicrobial properties of HF were shown against Staphylococcus aureus (MIC, MBC = 12.5 mg/mL). After exposing the endothelial cells to HF extract in vitro, it did not observed any change in the cell viability, proliferation, wound healing or proinflammatory cytokine production, but there was a dose dependent-reduction in ROS generation. In conclusion, HF extract exhibits anti-hyaluronidase activity, antimicrobial properties, and reduces ROS production. These observations may partly justify the use of HF in supporting wound healing.
Alnus glutinosa and A. incana (Betulaceae), are a small to medium size tree, native to the Northe... more Alnus glutinosa and A. incana (Betulaceae), are a small to medium size tree, native to the Northern Hemisphere. The leaves of this species are used in the Republic of Belarus as a source of antioxidants. The aim of this work was investigation of antioxidant activities and total phenolics content in various extracts obtained using water, mixture water with ethanol (from 9:1 to 2:8), and ethanol from A. glutinosa and A. incana leaves. Phenolics content was determined by method with Folin-Ciocalteu reagent and calculated on ellagic acid. The antioxidant activities were measured utilising 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical scavenging test. The highest phenolics concentration was observed in the extracts prepared by extraction with the mixture of water:ethanol from 7:3 to 3:7 and ranged from 17.82% to 18.96% for A. glutinosa and from 10.82% to 12.55% for A. incana. This extracts exhibited the highest free radical scavenging activity ranging from 49.21% to 49.42% and from 41.28% to 41.67% for A. glutinosa and A. incana respectively, comparable to the activity of quercetin. Therefore the mixture of water:ethanol from 7:3 to 3:7 should be used for preparing extracts from this species for medicinal purposes. Results also indicated the existence of a high correlation between antioxidant activity and total phenolics content.
Acta Physiologiae Plantarum, 2009
The gas chromatography-mass spectrometry (GC-MS) analysis of essential oil obtained by hydrodisti... more The gas chromatography-mass spectrometry (GC-MS) analysis of essential oil obtained by hydrodistillation from the flower of Taraxacum officinale L. revealed the presence of 25 compounds with 1,3-dimethylbenzene, 1,2-dimethylbenzene, 1-ethyl-3-methylbenzene, heneicosane and tricosane as the main components.
Acta Physiologiae Plantarum, 2001
Two flavonoid sulphates, i.e. quercetin 3-O-sulphate-7-O-aarabinopyranoside and kaempferol 3-O-su... more Two flavonoid sulphates, i.e. quercetin 3-O-sulphate-7-O-aarabinopyranoside and kaempferol 3-O-sulphate-7-O-a-arabinopyranoside, were isolated from leaves ofAtriplex hortensis L. The structures of these compounds were established by UV, 1H and 13C NMR, 2D NMR and MS spectra. The compounds were isolated for the first time from plant material.
Acta Chromatographica, 2008
Densitometric HPTLC and HPLC have been used for quantification of p-coumaric and protocatechuic a... more Densitometric HPTLC and HPLC have been used for quantification of p-coumaric and protocatechuic acids in an ethereal fraction from a methanolic extract of Aquilegia vulgaris L. HPLC analysis was performed on an RP-18 column with methanolwater-formic acid 25:75:0.5 (v/v) as mobile phase. Thin layer chromatography was performed on Si60 F 254 HPTLC plates with mixtures of heptane, dichloromethane, diisopropyl ether, formic acid, and water as mobile phases. Satisfactory separation of the phenolic acids was achieved by use of the multiple gradient development technique. The quantities of p-coumaric and protocatechuic acids determined by HPLC were 0.374 and 2.283 mg g −1 dry plant material, respectively; HPTLC results were somewhat higher-0.396 and 2.584 mg g −1 , respectively. The precision of both methods, expressed as relative standard deviation, was satisfactory. The methods are useful for quality control of Aquilegia vulgaris extracts.
Acta Biologica Cracoviensia Series Botanica, 2010
Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the an... more Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the antioxidant activity of three flavonoids with different polarity: 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside) and I 3' II 8 biapigenine (amentoflavone), with nonpolar all-trans β-carotene used as standard carotenoid molecule. The ABTS [2,2'azino-bis(3-ethylbenzthiazoline-6-sulphonic acid] cation radical decolorization assay was modified as follows: (1) measurements extended up to 8 days after preparation, (2) method adapted for flavonoids with different polarity and β-carotene, (3) concentrations in the 0.01-10 μM range of both trolox and antioxidants in order to use the same experimental conditions for both this technique and EPR measurement. K Ke ey y w wo or rd ds s: : 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside), I 3' II 8 biapigenine (amentoflavone), all-trans β-carotene, ABTS cation radical, TEAC, EPR technique, free radicals, oxidation.