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Papers by Wilaiporn Insuwan

Trends in Sciences

This study investigated the formulation of a product prototype RMUTI-SKC by using the legal part ... more This study investigated the formulation of a product prototype RMUTI-SKC by using the legal part of cannabis plants (leaves). Five product prototypes were composed of cannabis (leaves), stevia and mint. The herbs were added to improve the taste, smell and physical appearance properties of the product prototype which was yellowish-green. The analysis of the chemical properties found no heavy metals (Cd, As, and Pb) were observed (based on THAI community product standard, and demonstrated a moderate antioxidant activity, total phenolic content and total flavonoid content. The GC-MS profile of crude extracts analysis revealed the phytochemicals based on pharmacological action of essential oil, flavoring substance, anti-inflammatory, antioxidant, anti-bacterial, antidiabetic, antifungal and anti-cancer, respectively. The experimental results showed the pharmacological properties of the product prototype which can be a guideline for applying cannabis plants to other products. Further st...

Journal of Porous Materials, 2014

ABSTRACT This article investigated adsorption of acridine hydrochloride (Ac) and acriflavine hydr... more ABSTRACT This article investigated adsorption of acridine hydrochloride (Ac) and acriflavine hydrochloride (AF) on zeolite L (in form of K-LTL and H-LTL) including ice hockey and round shapes in order to explore new antenna materials. The amount of the adsorbed dyes and the interactions between the dyes and zeolite framework were examined by the adsorption isotherms study. The study revealed that the adsorption isotherms are fit very well with the Langmuir model. Moreover, the thermodynamic quantities indicated that the adsorptions of both dyes on zeolite L are spontaneous, endothermic and highly disordered. The maximum loading (Θmax) of AF and Ac on H-LTL and K-LTL with both shapes was in the range of 4.03–6.44 and 0.45–2.80 % by mole, respectively. The higher maximum loading of AF than that of Ac on zeolite L was explained in terms of the stronger interaction of AF with zeolite L than Ac. The morphology of zeolite L was found to have an effect on the adsorption of Ac and AF.

The objective of study was to perform a crude extract from leaves and flowers of Cassia fistula b... more The objective of study was to perform a crude extract from leaves and flowers of Cassia fistula by using two types of solvents; ethanol and hexane in order to reduce chemicals or antibiotics application. After studying phytochemical substances by testing color development and sedimentation in both fresh and dry forms, we found that fresh leaves had higher phytochemical properties than flowers. There were 8 important properties found; alkaloids, flavonoids, coumarin, tannins, flobatannins. terpenoids, steroids, and cardiac glycosides. Fresh leaves were used in protein form identification by using SDS-PAGE technique, the important protein found having molecular weight between 23 to 80 kDa consisting of seven bands; 80, 71, 52, 48, 40, 35, and 23 kDa, respectively. Moreover, their antibacterial activity was studied by using disc diffusion method and dilution method and found that the crude extract had inhibitory activity on Gram positive bacteria, Staphylococus aureus, and gram negati...

This study reports on removal of phenol on various solid adsorbents namely, activated carbon (AC)... more This study reports on removal of phenol on various solid adsorbents namely, activated carbon (AC), zeolite LTL in 2 forms, K-LTL and H-LTL. This work was performed by batch adsorption techniques. The effect of adsorbent, pH and temperature were determined to find out the optimal condition. The amounts of adsorbed phenol were interpreted using Langmuir and Freundlich. K-LTL zeolite was found to be most effective for the removal of phenol than H-LTL and AC. The optimum conditions for maximum adsorption were K-LTL being used as an adsorbent, at pH 4. Moreover, it was found that the adsorption was enhanced with increasing temperature. This results showed that the K-LTL zeolite exhibit a better an adsorbent for the removal of phenol from aqueous solutions. Furthermore, thermodynamic parameters including Gibbs free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) were also determined. The results indicating the adsorption is spontaneous and endothermic process. Keywords: Zeolite LTL, Activ...

Acetylcholinesterase (AChE) is classified as a specific biomarker for herbicide exposure in aquat... more Acetylcholinesterase (AChE) is classified as a specific biomarker for herbicide exposure in aquatic organisms. In this study, we investigated an AChE induction in the brain of juvenile and adult hybrid catfish after exposoure to glyphosate in sub-lethal concentration for quantifying the highest yield of AChE. Moreover, we separated and purified AChE by using a one-step column chromatography technique, which could be an alternative method. Based on the results, the appropriate glyphosate concentration for induction of AChE in juvenile catfish hybrids was 0.2 ml/ L while that in adult was 0.75 ml/L. And, hydroxyapatite was applied as a media for separation and 0.2 M phosphate buffer was applied for protein elution. After comparing the suitability of hybrid catfish for extracting AChE, it was found that adult hybrid catfish was more suitable than the juvenile. The molecular weight of separated AChE was 71 kDa when studied by using SDS-PAGE and Western blot techniques. For our results,...

Zeolite LTL (K_LTL and H_LTL) was used as adsorbent for the adsorption of herbicide paraquat from... more Zeolite LTL (K_LTL and H_LTL) was used as adsorbent for the adsorption of herbicide paraquat from an aqueous solution. The characterization of zeolite LTL has been carried out through various techniques: X-ray diffraction, X-ray fluorescence spectroscopy, Fourier transform infrared spectroscopy and surface analysis. The adsorption occurred through cation exchange and the results showed that the adsorption capacity of paraquat onto K_LTL (161.71 mg/g) is higher than H_LTL (25.67 mg/g). These results can be explained by the amount of Al in the structure of zeolite and affinities of negative sites in zeolite and extra-framework cations (K+ and H+). The equilibrium data was best adjusted using the Langmuir model which has high correlation coefficient values (0.99).

Two textile dye (indigo carmine and acid blue 25) were removed using activated carbon (AC) as sol... more Two textile dye (indigo carmine and acid blue 25) were removed using activated carbon (AC) as solid adsorbent. Effects of various parameters such as adsorbent dosage, contact time, kinetics and thermodynamic properties were investigated by batch adsorption technique. The results shown that the adsorption kinetics of textile dye was determined by the pseudo-second order model and adsorption isotherms fitted very well with Langmuir model. In addition, thermodynamic properties indicated that the adsorption of both textile dye on AC were endothermic and spontaneous process. The maximum adsorption capacities (qm) of indigo carmine and acid blue 25 onto AC was 89.29-104.17 mg/g and 1,428.00-2,000.00 mg/g, respectively. This results could be explained by stronger interaction between acid blue 25 and AC. This work indicated that AC can be used as an alternative adsorbent for removal of textile dye especially acid blue 25, due to the low cost and high efficiency of adsorption capacity. Keywo...

Journal of Porous Materials

We report a novel potential light-harvesting antenna material constructed with three dyes loaded ... more We report a novel potential light-harvesting antenna material constructed with three dyes loaded on two types of zeolite LTL (SiGe_LTL and Si_LTL). Fluorescence resonance energy transfer (FRET) occurs from acridine hydrochloride (Ac) to Thionine acetate (Th) via Acriflavine hydrochloride (AF), which acts as an antenna to receive and transfer energy from Ac to Th. We compared multistep FRET systems based on dye-loaded SiGe_LTL paired with an Si_LTL-based system. Our results show that in both cases, FRET efficiency increases with Th loading and decreases with increasing AF loading. Moreover, the zeolite LTL microenvironment causes a red shift in the fluorescence spectra of the three SiGe_LTL-loaded-dyes compared to those of the Si_LTL-based dyes.

Microporous and Mesoporous Materials

Microporous and Mesoporous Materials, Oct 1, 2014

Combined experimental-theoretical method is an effective tool to study dye–zeolite system.The con... more Combined experimental-theoretical method is an effective tool to study dye–zeolite system.The confinement of LTL zeolite affects isomerization of trans- to cis- azobenzene.Our model can accurately predict the optical properties of t-azobenzene in zeolite.The t-azobenzene adsorbed in H-LTL channel is protonated by Brønsted acid site of H-LTL.The t-azobenzene can be converted back to the cis-form with exposure to UV-light.The spectroscopic methods and molecular modeling were employed to study the photophysical properties of t-azobenzene in solution and in the channel of zeolite LTL. The effects of the interactions between the dye molecules and zeolite framework on the electronic states were examined experimentally by diffuse reflectance UV–visible absorption and fluorescence emission. The changes observed in the spectra were dependent on the forms of zeolite LTL (H-LTL and K-LTL). In addition the structural properties of the dye in the different environments were also intensively inve...

Microporous and Mesoporous Materials, 2014

Engineering Journal, 2012

Zeolite L is a crystalline aluminosilicate compound and a typical chemical composition of K9Al9Si... more Zeolite L is a crystalline aluminosilicate compound and a typical chemical composition of K9Al9Si27O72•nH2O (n = 0-36). The structure and chemical properties, as well as their sizes and morphologies of zeolite L has led to various applications in different fields. The aim of this study is to investigate the effects of chemical compositions of the starting gel on the synthesis, size and morphology of zeolite L crystals. Zeolite L had been synthesized hydrothermally at 180 ˚C for 2 days, from gels with the molar compositions of 2.62-3.78 K2O: 0.8-1.4 Al2O3: 8-12 SiO2: 80-200 H2O. The variation of chemical compositions led to the differences in morphologies and crystal sizes. Their morphologies varied from ice hockey to cylindrical shapes and their crystal sizes varying from 1.50-7.53 µm. With an increase in H2O and SiO2, the crystal size was also increased but decreased with an increase in K2O. In varying Al2O3, there was no effect on their shapes which were still cylindrical but with different crystal sizes. Moreover, the adsorption of ethylene on zeolite L samples depended significantly on crystal shapes and sizes.

This research work directly focuses on a new feasible light harvesting antenna material construct... more This research work directly focuses on a new feasible light harvesting antenna material constructed with Acridine hydrochloride (Ac)/Acriflavine hydrochloride (AF), as donor/acceptor for energy transfer, loaded on a round shape zeolite LTL (K-LTL and H-LTL). The energy transfer was monitored by absorption and fluorescence spectra while the calculated Forster distance (R DA ) and Quenching efficiency (%Q) of Ac/AF on K-LTL and H-LTL varied between 22.0 A to 19.6 A and 71.4% to 65.5%, respectively. Also, it was found that the microenvironment of a solid host such as K-LTL and H-LTL has significantly influenced the fluorescence spectra of Ac/AF on H-LTL approximately 50 nm longer than that on K-LTL.

Trends in Sciences

This study investigated the formulation of a product prototype RMUTI-SKC by using the legal part ... more This study investigated the formulation of a product prototype RMUTI-SKC by using the legal part of cannabis plants (leaves). Five product prototypes were composed of cannabis (leaves), stevia and mint. The herbs were added to improve the taste, smell and physical appearance properties of the product prototype which was yellowish-green. The analysis of the chemical properties found no heavy metals (Cd, As, and Pb) were observed (based on THAI community product standard, and demonstrated a moderate antioxidant activity, total phenolic content and total flavonoid content. The GC-MS profile of crude extracts analysis revealed the phytochemicals based on pharmacological action of essential oil, flavoring substance, anti-inflammatory, antioxidant, anti-bacterial, antidiabetic, antifungal and anti-cancer, respectively. The experimental results showed the pharmacological properties of the product prototype which can be a guideline for applying cannabis plants to other products. Further st...

Journal of Porous Materials, 2014

ABSTRACT This article investigated adsorption of acridine hydrochloride (Ac) and acriflavine hydr... more ABSTRACT This article investigated adsorption of acridine hydrochloride (Ac) and acriflavine hydrochloride (AF) on zeolite L (in form of K-LTL and H-LTL) including ice hockey and round shapes in order to explore new antenna materials. The amount of the adsorbed dyes and the interactions between the dyes and zeolite framework were examined by the adsorption isotherms study. The study revealed that the adsorption isotherms are fit very well with the Langmuir model. Moreover, the thermodynamic quantities indicated that the adsorptions of both dyes on zeolite L are spontaneous, endothermic and highly disordered. The maximum loading (Θmax) of AF and Ac on H-LTL and K-LTL with both shapes was in the range of 4.03–6.44 and 0.45–2.80 % by mole, respectively. The higher maximum loading of AF than that of Ac on zeolite L was explained in terms of the stronger interaction of AF with zeolite L than Ac. The morphology of zeolite L was found to have an effect on the adsorption of Ac and AF.

The objective of study was to perform a crude extract from leaves and flowers of Cassia fistula b... more The objective of study was to perform a crude extract from leaves and flowers of Cassia fistula by using two types of solvents; ethanol and hexane in order to reduce chemicals or antibiotics application. After studying phytochemical substances by testing color development and sedimentation in both fresh and dry forms, we found that fresh leaves had higher phytochemical properties than flowers. There were 8 important properties found; alkaloids, flavonoids, coumarin, tannins, flobatannins. terpenoids, steroids, and cardiac glycosides. Fresh leaves were used in protein form identification by using SDS-PAGE technique, the important protein found having molecular weight between 23 to 80 kDa consisting of seven bands; 80, 71, 52, 48, 40, 35, and 23 kDa, respectively. Moreover, their antibacterial activity was studied by using disc diffusion method and dilution method and found that the crude extract had inhibitory activity on Gram positive bacteria, Staphylococus aureus, and gram negati...

This study reports on removal of phenol on various solid adsorbents namely, activated carbon (AC)... more This study reports on removal of phenol on various solid adsorbents namely, activated carbon (AC), zeolite LTL in 2 forms, K-LTL and H-LTL. This work was performed by batch adsorption techniques. The effect of adsorbent, pH and temperature were determined to find out the optimal condition. The amounts of adsorbed phenol were interpreted using Langmuir and Freundlich. K-LTL zeolite was found to be most effective for the removal of phenol than H-LTL and AC. The optimum conditions for maximum adsorption were K-LTL being used as an adsorbent, at pH 4. Moreover, it was found that the adsorption was enhanced with increasing temperature. This results showed that the K-LTL zeolite exhibit a better an adsorbent for the removal of phenol from aqueous solutions. Furthermore, thermodynamic parameters including Gibbs free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) were also determined. The results indicating the adsorption is spontaneous and endothermic process. Keywords: Zeolite LTL, Activ...

Acetylcholinesterase (AChE) is classified as a specific biomarker for herbicide exposure in aquat... more Acetylcholinesterase (AChE) is classified as a specific biomarker for herbicide exposure in aquatic organisms. In this study, we investigated an AChE induction in the brain of juvenile and adult hybrid catfish after exposoure to glyphosate in sub-lethal concentration for quantifying the highest yield of AChE. Moreover, we separated and purified AChE by using a one-step column chromatography technique, which could be an alternative method. Based on the results, the appropriate glyphosate concentration for induction of AChE in juvenile catfish hybrids was 0.2 ml/ L while that in adult was 0.75 ml/L. And, hydroxyapatite was applied as a media for separation and 0.2 M phosphate buffer was applied for protein elution. After comparing the suitability of hybrid catfish for extracting AChE, it was found that adult hybrid catfish was more suitable than the juvenile. The molecular weight of separated AChE was 71 kDa when studied by using SDS-PAGE and Western blot techniques. For our results,...

Zeolite LTL (K_LTL and H_LTL) was used as adsorbent for the adsorption of herbicide paraquat from... more Zeolite LTL (K_LTL and H_LTL) was used as adsorbent for the adsorption of herbicide paraquat from an aqueous solution. The characterization of zeolite LTL has been carried out through various techniques: X-ray diffraction, X-ray fluorescence spectroscopy, Fourier transform infrared spectroscopy and surface analysis. The adsorption occurred through cation exchange and the results showed that the adsorption capacity of paraquat onto K_LTL (161.71 mg/g) is higher than H_LTL (25.67 mg/g). These results can be explained by the amount of Al in the structure of zeolite and affinities of negative sites in zeolite and extra-framework cations (K+ and H+). The equilibrium data was best adjusted using the Langmuir model which has high correlation coefficient values (0.99).

Two textile dye (indigo carmine and acid blue 25) were removed using activated carbon (AC) as sol... more Two textile dye (indigo carmine and acid blue 25) were removed using activated carbon (AC) as solid adsorbent. Effects of various parameters such as adsorbent dosage, contact time, kinetics and thermodynamic properties were investigated by batch adsorption technique. The results shown that the adsorption kinetics of textile dye was determined by the pseudo-second order model and adsorption isotherms fitted very well with Langmuir model. In addition, thermodynamic properties indicated that the adsorption of both textile dye on AC were endothermic and spontaneous process. The maximum adsorption capacities (qm) of indigo carmine and acid blue 25 onto AC was 89.29-104.17 mg/g and 1,428.00-2,000.00 mg/g, respectively. This results could be explained by stronger interaction between acid blue 25 and AC. This work indicated that AC can be used as an alternative adsorbent for removal of textile dye especially acid blue 25, due to the low cost and high efficiency of adsorption capacity. Keywo...

Journal of Porous Materials

We report a novel potential light-harvesting antenna material constructed with three dyes loaded ... more We report a novel potential light-harvesting antenna material constructed with three dyes loaded on two types of zeolite LTL (SiGe_LTL and Si_LTL). Fluorescence resonance energy transfer (FRET) occurs from acridine hydrochloride (Ac) to Thionine acetate (Th) via Acriflavine hydrochloride (AF), which acts as an antenna to receive and transfer energy from Ac to Th. We compared multistep FRET systems based on dye-loaded SiGe_LTL paired with an Si_LTL-based system. Our results show that in both cases, FRET efficiency increases with Th loading and decreases with increasing AF loading. Moreover, the zeolite LTL microenvironment causes a red shift in the fluorescence spectra of the three SiGe_LTL-loaded-dyes compared to those of the Si_LTL-based dyes.

Microporous and Mesoporous Materials

Microporous and Mesoporous Materials, Oct 1, 2014

Combined experimental-theoretical method is an effective tool to study dye–zeolite system.The con... more Combined experimental-theoretical method is an effective tool to study dye–zeolite system.The confinement of LTL zeolite affects isomerization of trans- to cis- azobenzene.Our model can accurately predict the optical properties of t-azobenzene in zeolite.The t-azobenzene adsorbed in H-LTL channel is protonated by Brønsted acid site of H-LTL.The t-azobenzene can be converted back to the cis-form with exposure to UV-light.The spectroscopic methods and molecular modeling were employed to study the photophysical properties of t-azobenzene in solution and in the channel of zeolite LTL. The effects of the interactions between the dye molecules and zeolite framework on the electronic states were examined experimentally by diffuse reflectance UV–visible absorption and fluorescence emission. The changes observed in the spectra were dependent on the forms of zeolite LTL (H-LTL and K-LTL). In addition the structural properties of the dye in the different environments were also intensively inve...

Microporous and Mesoporous Materials, 2014

Engineering Journal, 2012

Zeolite L is a crystalline aluminosilicate compound and a typical chemical composition of K9Al9Si... more Zeolite L is a crystalline aluminosilicate compound and a typical chemical composition of K9Al9Si27O72•nH2O (n = 0-36). The structure and chemical properties, as well as their sizes and morphologies of zeolite L has led to various applications in different fields. The aim of this study is to investigate the effects of chemical compositions of the starting gel on the synthesis, size and morphology of zeolite L crystals. Zeolite L had been synthesized hydrothermally at 180 ˚C for 2 days, from gels with the molar compositions of 2.62-3.78 K2O: 0.8-1.4 Al2O3: 8-12 SiO2: 80-200 H2O. The variation of chemical compositions led to the differences in morphologies and crystal sizes. Their morphologies varied from ice hockey to cylindrical shapes and their crystal sizes varying from 1.50-7.53 µm. With an increase in H2O and SiO2, the crystal size was also increased but decreased with an increase in K2O. In varying Al2O3, there was no effect on their shapes which were still cylindrical but with different crystal sizes. Moreover, the adsorption of ethylene on zeolite L samples depended significantly on crystal shapes and sizes.

This research work directly focuses on a new feasible light harvesting antenna material construct... more This research work directly focuses on a new feasible light harvesting antenna material constructed with Acridine hydrochloride (Ac)/Acriflavine hydrochloride (AF), as donor/acceptor for energy transfer, loaded on a round shape zeolite LTL (K-LTL and H-LTL). The energy transfer was monitored by absorption and fluorescence spectra while the calculated Forster distance (R DA ) and Quenching efficiency (%Q) of Ac/AF on K-LTL and H-LTL varied between 22.0 A to 19.6 A and 71.4% to 65.5%, respectively. Also, it was found that the microenvironment of a solid host such as K-LTL and H-LTL has significantly influenced the fluorescence spectra of Ac/AF on H-LTL approximately 50 nm longer than that on K-LTL.