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Papers by Wilber Quispe Mendoza

vrinvestigacion.unmsm.edu.pe

Hoy en día nadie puede desconocer que la ciencia y la aplicación del conocimiento científico, est... more Hoy en día nadie puede desconocer que la ciencia y la aplicación del conocimiento científico, esto es la tecnología, tienen juntas un papel importante que cumplir en la vida de la sociedad. Recientes estudios avizoran el inicio de una nueva era tecnológica, de manera que si no nos preparamos corremos el riesgo de quedar marginados de los procesos de cambio en el actual desarrollo.

Infection, Genetics and …, Jan 1, 2005

Multi-locus enzyme electrophoresis is the current gold standard for the genetic characterisation ... more Multi-locus enzyme electrophoresis is the current gold standard for the genetic characterisation of Leishmania. However, this method is time-consuming and, more importantly, cannot be directly applied to parasites present in host tissue. PCR-based methods represent an ideal alternative but, to date, a multi-locus analysis has not been applied to the same sample. This has now been achieved with a sample of 55 neotropical isolates (Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, L. (V.) lainsoni and L. (L.) amazonensis), using five different genes as targets, four of which encoded major Leishmania antigens (gp63, Hsp70, H2B and Cpb). Our multi-locus approach strongly supports the current taxonomy and demonstrates a highly robust method of distinguishing different strains. Within L. (V.) braziliensis, we did not encounter so far specific genetic differences between parasites isolated from cutaneous and mucosal lesions. Interestingly, results provided by each of the different antigen-genes in the species considered, were different, suggesting different selective pressures. Our work emphasises the need for a multi-disciplinary approach to study the clinical pleomorphism of leishmaniasis. #

Cell host & …, Jan 1, 2009

Plasmodium development within Anopheles mosquitoes is a vulnerable step in the parasite transmiss... more Plasmodium development within Anopheles mosquitoes is a vulnerable step in the parasite transmission cycle, and targeting this step represents a promising strategy for malaria control. The thioester-containing complement-like protein TEP1 and two leucine-rich repeat (LRR) proteins, LRIM1 and APL1, have been identified as major mosquito factors that regulate parasite loads. Here, we show that LRIM1 and APL1 are required for binding of TEP1 to parasites. RNAi silencing of the LRR-encoding genes results in deposition of TEP1 on Anopheles tissues, thereby depleting TEP1 from circulation in the hemolymph and impeding its binding to Plasmodium. LRIM1 and APL1 not only stabilize circulating TEP1, they also stabilize each other prior to their interaction with TEP1. Our results indicate that three major antiparasitic factors in mosquitoes jointly function as a complement-like system in parasite killing, and they reveal a role for LRR proteins as complement control factors.

Page 1. Fecha de inicio: sábado 12-diciembre-2009 Tema de Exposición N° Apellidos y Nombres Indic... more Page 1. Fecha de inicio: sábado 12-diciembre-2009 Tema de Exposición N° Apellidos y Nombres Indicaciones 6. Organización funcional Implementación de datapaths simples ... N° de transistores Pentium IV: Cedar Mill, M y D 48 PILLPE HUAMÁN, Henry Miguel Voltaje core ...

Revista de …, Jan 1, 2012

Rol del óxido nítrico en la hipertrofia arteriolar pulmonar y ventricular cardiaca derecha en pol... more Rol del óxido nítrico en la hipertrofia arteriolar pulmonar y ventricular cardiaca derecha en pollos a nivel del mar y expuestos a hipoxia de la altura/The role of nitric oxide in pulmonary arteriolar and left heart ventricle hypertrophy in chickens at sea level and exposed to high altitude hypoxia

vrinvestigacion.unmsm.edu.pe

Hoy en día nadie puede desconocer que la ciencia y la aplicación del conocimiento científico, est... more Hoy en día nadie puede desconocer que la ciencia y la aplicación del conocimiento científico, esto es la tecnología, tienen juntas un papel importante que cumplir en la vida de la sociedad. Recientes estudios avizoran el inicio de una nueva era tecnológica, de manera que si no nos preparamos corremos el riesgo de quedar marginados de los procesos de cambio en el actual desarrollo.

Infection, Genetics and …, Jan 1, 2005

Multi-locus enzyme electrophoresis is the current gold standard for the genetic characterisation ... more Multi-locus enzyme electrophoresis is the current gold standard for the genetic characterisation of Leishmania. However, this method is time-consuming and, more importantly, cannot be directly applied to parasites present in host tissue. PCR-based methods represent an ideal alternative but, to date, a multi-locus analysis has not been applied to the same sample. This has now been achieved with a sample of 55 neotropical isolates (Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, L. (V.) lainsoni and L. (L.) amazonensis), using five different genes as targets, four of which encoded major Leishmania antigens (gp63, Hsp70, H2B and Cpb). Our multi-locus approach strongly supports the current taxonomy and demonstrates a highly robust method of distinguishing different strains. Within L. (V.) braziliensis, we did not encounter so far specific genetic differences between parasites isolated from cutaneous and mucosal lesions. Interestingly, results provided by each of the different antigen-genes in the species considered, were different, suggesting different selective pressures. Our work emphasises the need for a multi-disciplinary approach to study the clinical pleomorphism of leishmaniasis. #

Cell host & …, Jan 1, 2009

Plasmodium development within Anopheles mosquitoes is a vulnerable step in the parasite transmiss... more Plasmodium development within Anopheles mosquitoes is a vulnerable step in the parasite transmission cycle, and targeting this step represents a promising strategy for malaria control. The thioester-containing complement-like protein TEP1 and two leucine-rich repeat (LRR) proteins, LRIM1 and APL1, have been identified as major mosquito factors that regulate parasite loads. Here, we show that LRIM1 and APL1 are required for binding of TEP1 to parasites. RNAi silencing of the LRR-encoding genes results in deposition of TEP1 on Anopheles tissues, thereby depleting TEP1 from circulation in the hemolymph and impeding its binding to Plasmodium. LRIM1 and APL1 not only stabilize circulating TEP1, they also stabilize each other prior to their interaction with TEP1. Our results indicate that three major antiparasitic factors in mosquitoes jointly function as a complement-like system in parasite killing, and they reveal a role for LRR proteins as complement control factors.

Page 1. Fecha de inicio: sábado 12-diciembre-2009 Tema de Exposición N° Apellidos y Nombres Indic... more Page 1. Fecha de inicio: sábado 12-diciembre-2009 Tema de Exposición N° Apellidos y Nombres Indicaciones 6. Organización funcional Implementación de datapaths simples ... N° de transistores Pentium IV: Cedar Mill, M y D 48 PILLPE HUAMÁN, Henry Miguel Voltaje core ...

Revista de …, Jan 1, 2012

Rol del óxido nítrico en la hipertrofia arteriolar pulmonar y ventricular cardiaca derecha en pol... more Rol del óxido nítrico en la hipertrofia arteriolar pulmonar y ventricular cardiaca derecha en pollos a nivel del mar y expuestos a hipoxia de la altura/The role of nitric oxide in pulmonary arteriolar and left heart ventricle hypertrophy in chickens at sea level and exposed to high altitude hypoxia