Wilfrida Decraemer - Academia.edu (original) (raw)

Papers by Wilfrida Decraemer

Fundamental and applied nematology, 1992

-SEM studies of the unusual species Criconema paradoxiger (Orton Williams, 1982) Raski & Luc, 198... more -SEM studies of the unusual species Criconema paradoxiger (Orton Williams, 1982) Raski & Luc, 1985 revealed new aspects of the cuticular structure in juveniles. A new species Ogma paracivellae n. sp. is described; it differs from 0. civellae (Steiner, 1949) Raski & Luc, 1987 by its larger number of body rings (R = 62-70 vs 40-55) and, in juveniles, by the presence of a higher number of scales per annulus (twelve to eighteen vs eight). Résumé-Crïconema paradoxiger, Ogma civellae et O. paracivellae sp. n. provenant de Papouasie-Nouvelle-Guinée (Nemata : Tylenchida)-Une étude au MEB d'une espèce inusuelle, Criconema paradoxiger (Orton Williams, 1982) Raski & Luc, 1985, a révélé de nouveaux détails sur la cuticule des juvéniles. Une nouvelle espéce, Ogma paracivellae n. sp., est décrite. Elle différe de 0. civellae (Steiner, 1949) Raski & Luc, 1987 par le plus grand nombre d'anneaux du corps (R = 62-70 vs 40-55) et, chez les juvéniles, par un nombre plus élevé d'écailles sur chaque anneau (douze à dix-huit contre huit).

Gent Academia Press eBooks, 2009

CABI eBooks, 2013

This chapter presents the classification, biochemical and molecular diagnostics and management of... more This chapter presents the classification, biochemical and molecular diagnostics and management of ectoparasitic nematodes, including those belonging to Chromadoria and Enoplea (Longidoridae and Trichodoridae). Ectoparasitic tylenchs sensu stricto can be grouped according to their parasitic strategies: (i) the migratory ectoparasites, which stay vermiform throughout their life cycle and feed for short periods along the root system (e.g. Trichodorus, Belonolaimus, Dolichodorus); and (ii) the sedentary ectoparasites, which are those species that may feed for several days on the same cell, either a cortical or an epidermal cell. The Longidoridae and Trichodoridae are migratory root ectoparasites. They are responsible for substantial direct damage to a wide variety of plants, but their major pest status is as virus vectors, despite the rather low number of vector species in both families.

Fundamental and applied nematology, 1996

CBM - Cahiers de Biologie Marine, 2000

Three new species of the genus Leptepsilonema and one new species of Polkepsilonema are described... more Three new species of the genus Leptepsilonema and one new species of Polkepsilonema are described. Leptepsilonema antonioi sp. nov. is mainly characterized by 111-115 overlapping annules with a large vacuolar ornamentation, a large body size and in male, by number and arrangement of copulatory thorns; L. dauvini sp. nov. has 149-154 annules, ornamented with numerous fine vacuoles and fine outer ridges, and males possess a field of fine copulatory thorns at level of posterior ambulatory region and five minute thorns precloacally; L.. horridum sp. nov. is characterized by 130-131 annules, ornamented with large vacuoles and spines, and males with copulatory thorns arranged in four subventral rows, the inner rows with five or six well developed thorns followed at some distance by one to two thorns, and long spicules (67-72 µm). Polkepsilonema guirali sp. nov. is characterized by the number and location of copulatory thorns, the number of subcephalic setae, and the feature of the dorsal ...

Nematodes from phytotelmata of Nepenthes hamiguitanensis and N. peltata in Mt. Hamiguitan, Philip... more Nematodes from phytotelmata of Nepenthes hamiguitanensis and N. peltata in Mt. Hamiguitan, Philippines included three new species of the genera: Molgolaimus Ditlevsen 1921, Dominicactinolaimus Jairajpuri and Ahmad 1992, Tripylella (Bütschli, 1873) Brzeski & WiniszewskaŚlipińska, 1993; two known: Tylocephalus auriculatus (Bütschli, 1873) Anderson, 1966, Pelodera strongyloides (Schneider, 1860) Schneider 1866; and three uncertain species of the genera: Paractinolaimus Meyl 1957, Plectus Bastian 1865, and Anaplectus De Coninck & Schuurmans Stekhoven 1933. Measurements and illustrations are provided. Molgolaimus sp. nov. is characterized by the absence of pre-cloacal supplements, shape of the spicule with lamina widened distally, conical tail with swollen tip and without digitate prolongation, and sexual dimorphism in the shape of the cardia (elongated in male and more round in females). Moreover, a comprehensive key for the genus Molgolaimus is presented. Dominicactinolaimus sp. nov. i...

Thorp and Covich's Freshwater Invertebrates, 2019

Crop Protection, 2021

Abstract We investigated the use of entomopathogenic nematodes to biologically control Bactrocera... more Abstract We investigated the use of entomopathogenic nematodes to biologically control Bactrocera dorsalis in mango orchards. One isolate of Steinernema kandii (Thui) and two of Heterorhabditis taysearae (Hessa1 and Korobororou F4) were studied for their invasion time and virulence to third instar larvae of B. dorsalis in laboratory and semi field tests, respectively. In addition, the persistence of the same nematode isolates in soil under field conditions was tested. Results showed that all three nematode isolates could penetrate insect larvae after 2 h of exposure time. Furthermore, under semi field conditions, insect mortality was significantly different among EPN application times. The three nematode isolates were highly pathogenic to B. dorsalis with H. taysearae Hessa1 being the most virulent (70.84% ± 10.46 [SEM] mortality) when EPNs were applied three days before insect introduction in the experimental pots. Moreover, Steinernema kandii persisted in soil up to 32 weeks after nematode application whereas both H. taysearae isolates persisted 30 weeks post application in the mango orchard. In general, four weeks upon nematode application, the density of infective juveniles decreased considerably and remained variable the following sampling dates.

Nematology, 2015

Molecular data on several species of trichodorids occurring in Iran are provided. Live specimens ... more Molecular data on several species of trichodorids occurring in Iran are provided. Live specimens of two species, Trichodorus arasbaranensis and T. gilanensis, were collected from their type localities. One population of T. orientalis was collected from Arasbaran forests in Eastern Azarbayjan province. The species Paratrichodorus porosus, occurring in Gilan province, and one European isolate of P. pachydermus were also included in phylogenetic analysis. An Iranian population of T. minzi, representing only the second population found of this rare species, was recovered from soil samples collected from grasslands of north-western Iran and is in morphological congruence with the original description of the species. Besides morphological study, molecular phylogenetic study was performed on newly reported species using partial 28S rDNA data, and the phylogenetic relations of all sequenced species as inferred from Bayesian analysis are discussed. According to the phylogenetic tree, two new...

Biological Control, 2018

Bactrocera dorsalis fruit fly is the economically most significant tephritid pest species on Mang... more Bactrocera dorsalis fruit fly is the economically most significant tephritid pest species on Mango, Mangifera indica L., in Benin, and entomopathogenic nematodes (EPNs) represent good candidates for its control in the soil. In this study, the susceptibility of larvae and pupae of B. dorsalis to 12 EPN isolates originating from Benin was investigated. The effect of nematode concentrations (20, 50, 100, 200 and 300 Infective Juveniles (IJs)/ B. dorsalis larva) and of different substrate moisture content (10, 15, 20, 25 and 30% v/w) on B. dorsalis mortality at the larval stage was studied. Also, the reproduction potential inside B. dorsalis larvae was assessed. Our results revealed that the susceptibility of B. dorsalis larvae was significantly different among the 12 tested nematode isolates with H. taysearae isolate Azohoue2 causing the greatest insect mortality (96.09±1.44%). The lowest insect mortality (7.03±4.43%) was recorded with Steinernema sp. strain Bembereke. Significant differences in insect mortality were recorded when EPNs were applied at varying IJs concentrations. A concentration of 100 nematodes of either H. taysearae Azohoue2 or H. taysearae Hessa1 per B. dorsalis larva was enough to kill at least 90% of B. dorsalis larvae. Larvae were less susceptible to nematodes at higher moisture content (25% and 30%). In addition, pupae were less susceptible to nematodes than larvae. Furthermore, the tested nematode isolates were able to reproduce inside B. dorsalis third instar larva or pupa with the Heterorhabditis isolates giving the greatest multiplication rate (59577.2 IJs ± 14307.41).

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Nematology, 2019

Summary The stubby root nematodes are world-wide distributed polyphagous root ectoparasites and c... more Summary The stubby root nematodes are world-wide distributed polyphagous root ectoparasites and can cause damage to a wide range of crops and natural vegetation. In this study, 22 valid and putative species of stubby root nematodes were identified in 37 samples collected in Malaysia, Mexico, Russia, and the USA (California, Florida, Minnesota and Nevada). The analysis of 58 new sequences of the D2-D3 expansion fragments of 28S rRNA gene revealed the following species: Nanidorus minor from California, Florida and Mexico, N. renifer from Florida, two unidentified Nanidorus species from California and Malaysia, respectively, Paratrichodorus allius from Florida and Minnesota, P. pachydermus and Paratrichodorus sp. from Russia, Trichodorus californicus, T. intermedius, and T. obscurus from California, T. obtusus from Florida, eight unidentified Trichodorus species from California, one unidentified Trichodorus species from Nevada, and two unidentified species of stubby root nematodes, one...

Marine Biodiversity, 2017

A new genus of the family Desmodoridae, Cornurella gen. n. is described from a Campos Basin sea s... more A new genus of the family Desmodoridae, Cornurella gen. n. is described from a Campos Basin sea site, Rio de Janeiro, Brazil. It differs from the other genera of the family by the clearly annulated body cuticle, ornamented with eight longitudinal rows of spines, a strong cephalic capsule with thorn-like ornamentation; amphideal fovea closed loopshaped in both sexes but slightly smaller in female and located on a cuticular plate; precloacal region in male with setae and papillae intermingled; spicules long filiform and curved and gubernaculum without dorsal apophyses. The new genus was considered most closely related to Desmodorella because both genera share the strong cuticular annulations of the body wall and ornamentation with longitudinal rows of spines, but differs in the number of rows and the well-defined cephalic capsule ornamented with a dorsolateral pair and a ventrolateral pair of horn-like protrusions at its base. Other similarities between both genera are the sexual dimorphism in amphideal fovea and the presence of one dorsal and two ventro-sublateral teeth.

A study on the biodiversity of entomopathogenic nematodes was conducted during 2010 and 2011 in S... more A study on the biodiversity of entomopathogenic nematodes was conducted during 2010 and 2011 in South Benin. Soil samples from eight sites production of annual and perennial crops were analysed. We obtained 13.21 % of positive soil samples out of 280. We here report on the identification of six of these isolates. Molecular, morphometrical and morphological observations classified the isolates within the genus Heterorhabditis ; one isolate was conspecific with H. indica and two other isolates with H. sonorensis. More information is needed for effective identification of the remaining three isolates. Phylogenic analysis based on sequences of ITS regions of rDNA grouped our isolates with H. sonorensis and H. taysearae with bootstrap support values of 94 and 99 % in Maximum Parsimony and Neighbour Joining trees, respectively. Morphological characters of the infective juveniles and males did not correspond to those of H. taysearae, but were close to H. sonorensis. In contrast, the female...

Nematology, 2010

Molecular taxonomy is one of the most promising yet challenging fields of biology. Molecular mark... more Molecular taxonomy is one of the most promising yet challenging fields of biology. Molecular markers such as nuclear and mitochondrial genes are being used in a variety of studies surveying marine nematode taxa. Sequences from more than 600 species have been deposited to date in online databases. These barcode sequences are assigned to 150 nominal species from 104 genera. There are 41 species assigned to Enoplea and 109 species to Chromadorea. Morphology-based surveys are greatly limited by processing speed, while barcoding approaches for nematodes are hampered by difficulties in matching sequence data with morphology-based taxonomy. DNA barcoding is a promising approach because some genes contain variable regions that are useful to discriminate species boundaries, discover cryptic species, quantify biodiversity and analyse phylogeny. We advocate a combination of several approaches in studies of molecular taxonomy, DNA barcoding and conventional taxonomy as a necessary step to enhan...

Os Trichodoridae são nematóides ectoparasitas de plantas, polífagos, que ocorrem com uma ampla di... more Os Trichodoridae são nematóides ectoparasitas de plantas, polífagos, que ocorrem com uma ampla distribuição mundial. Apesar da família ser pequena em número de gêneros e espécies quando comparada com outros taxons de nematóides parasitas das plantas, o grupo não é menos importante. Os Trichodoridae incluem não só espécies que podem causar danos diretos em numerosas plantas, como também, várias espécies que atuam como vetores naturais de tobravírus às plantas. Estes nematóides vetores de vírus têm recebido maior atenção por parte dos especialistas nas últimas décadas. A família Trichodoridae consiste em apenas cinco gêneros: Trichodorus, Paratrichodorus, Monotrichodorus, Allotrichodorus e Ecuadorus. As espécies vetoras de vírus têm sido registradas apenas nos gêneros didélficos, Trichodorus e Paratrichodorus, os quais têm uma ampla distribuição mundial. Os três gêneros monodélficos têm sido referidos, até hoje, apenas nas regiões neotropicais da América Central e do Sul. Na família Trichodoridae as espécies podem ser endêmicas, tal como a maioria das espécies da África do Sul, enquanto outras, como Paratrichodorus minor, se encontram amplamente distribuídas. Atualmente, no Brasil são conhecidas apenas 13 espécies (P.

Nematologia …, 2006

... MATERIAL AND METHODS The salt-affected area of Cojocna (46º44'N, 23º50'E) is situat... more ... MATERIAL AND METHODS The salt-affected area of Cojocna (46º44'N, 23º50'E) is situated at 335 m altitude, 25 km south-east from Cluj-Napoca, in a region of deforested hills, typical of the Transylvanian Plain (Rusu et al., 1967). ... prostrata . + . . . Spergularia marina + . . . . ...