Christopher Wilkinson - Academia.edu (original) (raw)
Uploads
Papers by Christopher Wilkinson
Cell Biology International, 1995
We studied the influence of substrata topography on the behaviour of murine P388D1 macrophage cel... more We studied the influence of substrata topography on the behaviour of murine P388D1 macrophage cell line. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves of varying depth and width. Cell spread area, elongation, orientation and F-actin content were measured on plain substratum and 6 sets of gratings. The speed and persistence of cell movement were also studied.We found that patterned substrata substantially activated cell spreading and elongation and significantly increased the persistence and speed of cell movement, shallow grooves being more effective than deep ones. The contact of cells with micropatterned substrata significantly increased the F-actin content in cells. The sensitivity of LPS (lipopolisaccharide) stimulated and unstimulated macrophages to topographical cues was also compared.
The physical and molecular biological bases of the reactions of cells to features of the topograp... more The physical and molecular biological bases of the reactions of cells to features of the topography of the substratum or environment on which and in which cells live, both in culture and in the embryo, are discussed. The fact that most, if not all, cells react to micrometric and nanometric topography is stressed. Some cell types will react to steps as shallow as 11 nm. Methods of fabricating such topographies in a variety of materials are outlined. Types of topography and the reactions of cells to these are described. It is emphasized that different cell types are sensitive to fairly specific ranges of size of topography. Reactions to topography include cell orientation, changes in cell motility, cell adhesion and cell shape. The term 'contact guidance' has been used in this field, but the term 'topographic reaction' is more appropriate, since it covers the wide range of reactions that are reported. In addition, the reactions involve activation of tyrosine kinases, cytoskeletal condensation and further downstream activation and inactivation of gene expression. The reactions to topography are probably due to stretch reactions of the cells to the substratum and not to chemical details of the substratum. The reasons for this are that a given cell type reacts in much the same way to the same topography made with different materials and that, when both chemical patterns and topographic ones are offered to cells, topography tends to have a greater effect than chemical patterns.
Cell Biology International, 1995
We studied the influence of substrata topography on the behaviour of murine P388D1 macrophage cel... more We studied the influence of substrata topography on the behaviour of murine P388D1 macrophage cell line. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves of varying depth and width. Cell spread area, elongation, orientation and F-actin content were measured on plain substratum and 6 sets of gratings. The speed and persistence of cell movement were also studied.We found that patterned substrata substantially activated cell spreading and elongation and significantly increased the persistence and speed of cell movement, shallow grooves being more effective than deep ones. The contact of cells with micropatterned substrata significantly increased the F-actin content in cells. The sensitivity of LPS (lipopolisaccharide) stimulated and unstimulated macrophages to topographical cues was also compared.
The physical and molecular biological bases of the reactions of cells to features of the topograp... more The physical and molecular biological bases of the reactions of cells to features of the topography of the substratum or environment on which and in which cells live, both in culture and in the embryo, are discussed. The fact that most, if not all, cells react to micrometric and nanometric topography is stressed. Some cell types will react to steps as shallow as 11 nm. Methods of fabricating such topographies in a variety of materials are outlined. Types of topography and the reactions of cells to these are described. It is emphasized that different cell types are sensitive to fairly specific ranges of size of topography. Reactions to topography include cell orientation, changes in cell motility, cell adhesion and cell shape. The term 'contact guidance' has been used in this field, but the term 'topographic reaction' is more appropriate, since it covers the wide range of reactions that are reported. In addition, the reactions involve activation of tyrosine kinases, cytoskeletal condensation and further downstream activation and inactivation of gene expression. The reactions to topography are probably due to stretch reactions of the cells to the substratum and not to chemical details of the substratum. The reasons for this are that a given cell type reacts in much the same way to the same topography made with different materials and that, when both chemical patterns and topographic ones are offered to cells, topography tends to have a greater effect than chemical patterns.