T. Wirth - Academia.edu (original) (raw)

Papers by T. Wirth

Journal of Biological Chemistry, 1999

Interleukin-5 (IL-5), expressed primarily by type-2 T helper (Th2) cells, plays an important role... more Interleukin-5 (IL-5), expressed primarily by type-2 T helper (Th2) cells, plays an important role in the development of allergic diseases, such as allergic asthma. Studying the regulation of IL-5 gene expression by Ets transcription factors, we found that Ets1 and Ets2, but not Elf-1, were able to activate the human IL-5 promoter in Jurkat T-cells. This required the presence of either phorbol 12-myristate acetate (PMA) plus ionomycin or PMA plus the viral protein HTLV-I Tax 1. By mutation studies, it could be shown that Ets1 and Ets2 exerted their effects on the IL-5 promoter through a GGAA motif within the Cle0 element. In myeloid Kasumi cells, Ets1 and Ets2 failed to stimulate IL-5 promoter activity, unless the T-cell specific transcription factor GATA3 was added. These results show, for the first time, that Ets1 and Ets2 are able to cooperate with GATA3. Both ionomycin and Tax 1 increased the combined effect of GATA3 with Ets1 and Ets2 in the presence of PMA. The data further demonstrate that, in addition to Ets1, Ets2 is also able to functionally cooperate with Tax 1. The synergism of GATA3 with either Ets1 or Ets2 may play an important role in calcium-or Tax 1-dependent regulation of IL-5 expression in Th2 cells or in HTLV-I transformed adult T-cell leukemia cells, respectively.

Current Opinion in Immunology, 1998

Development, differentiation and cell death in all organisms are mediated by tightly regulated pr... more Development, differentiation and cell death in all organisms are mediated by tightly regulated programs of differential gene expression. Furthermore, changes in gene expression profiles are responsible for tumour formation and tumour progression, as well as for many other human diseases. Thus, the definition of distinct patterns of gene expression is not only essential for understanding complex biological processes but also leads to the identification of novel targets for therapy of various diseases.

Journal of Biological Chemistry, 1998

Nucleic Acids Research, 1991

Proceedings of the …, 2000

NF-κB is regulated by inhibitor proteins (IκBs), which retain NF-κB in the cytoplasm. Signal-indu... more NF-κB is regulated by inhibitor proteins (IκBs), which retain NF-κB in the cytoplasm. Signal-induced phosphorylation by the IκB-kinase complex containing the IκB-kinases 1 and 2 (IKK-1/2 or IKK-α/β) and subsequent degradation of the IκB proteins are prerequisites for NF-κB ...