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Papers by Wissem Aidi Wannes
Journal of exploratory research in pharmacology, Mar 11, 2022
Background and objectives: Rosemary (Rosmarinus officinalis L.) extract is widely used in food, c... more Background and objectives: Rosemary (Rosmarinus officinalis L.) extract is widely used in food, cosmetic and pharmaceutical industries. This study centered on the optimization of ethanolic extraction of rosemary leaves and evaluated the bioactivity of the extract. Methods: The optimized rosemary extract was analyzed using high-performance liquid chromatography. The antioxidant activity of the rosemary extract was measured using 2,2′-Azinobis-(3-ethylBenzoThiazoline-6-Sulfonic) acid, 2,2-DiPhenyl-1-Picryl-Hydrazyl-hydrate, and ferric reducing antioxidant power assays. The antibacterial activity of the rosemary extract was tested using the disk diffusion method. Toxicity was tested using mice. Nitrite production by RAW 264.7 cells was used to determine the anti-inflammatory activity of the rosemary extract. The construction and statistical analysis of the experimental design was done using NemrodW (LPRAI, version 2000) software. Results: We found that ethanol extraction of rosemary leaves depended on optimizing heating temperature and time. The maximum yields of carnosic and rosmarinic acids were obtained after optimizing these parameters according to the modelling software. The rosemary leaf extract had high antioxidant, anti-inflammatory, and antibacterial properties with low acute toxicity. Conclusions: Ethanol extraction of rosemary leaves could potentially be used in several industrial applications.
Le myrte (Myrtus communis L.), arbrisseau perenne spontane de la famille des Myrtacees, est tres ... more Le myrte (Myrtus communis L.), arbrisseau perenne spontane de la famille des Myrtacees, est tres connu par ses nombreuses vertus medicinales, nutritionnelles et ses nombreuses utilisations culinaires ce qui intensifie sa demande par les industries agroalimentaire et pharmaceutique. L’existence de deux varietes de myrte (Myrtus communis italica L. et Myrtus communis baetica L.) dans la flore de la Tunisie nous a incite a realiser ce travail de recherche afin d'evaluer biochimiquement la richesse des differents organes de ces deux varietes en composes bioactifs tels que les huiles essentielles, les acides gras et les polyphenols et de determiner leur activite antioxydante.
Journal of Food Biochemistry, 2010
Journal of Complementary and Integrative Medicine
Cancer is a major public health problem in the world. The use of the medicinal plants in cancer p... more Cancer is a major public health problem in the world. The use of the medicinal plants in cancer prevention and management is frequent in Africa, especially in Tunisia, and it is transmitted from generation to generation within cultures. Many previous studies showed that a wide range of Tunisian medicinal plants exerted cytotoxic and anticancer activity. A comprehensive review was conducted to collect information from scientific journal articles, including indigenous knowledge researches, about Tunisian medicinal plants used for the prevention and management of cancer. The aim of this review article is to provide the reader with information concerning the importance of Tunisian medicinal plants in the prevention and management of cancer and to open the door for the health professionals and scientists working in the field of pharmacology and therapeutics to produce new drug formulations to treat different types of cancer.
The effect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms o... more The effect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of their essential oils, phenolics and antioxidant activities. The optimum yield of essential oil was found in shade drying (2.62%). Fourteen volatile compounds were determined in all samples with variation of the main component proportions depending on drying methods. Thus, trans-anethole (84.21%) and estragole (3.82%) proportions significantly increased in shade drying. The highest total phenol and flavonoid contents of aniseeds were recorded in shade drying (42.70 mg of GAE/g and 53.55 mg of QE/g, respectively) while the lowest contents in oven drying at 60 °C (31.15 mg of GAE/g and 46.20 mg of QE/g, respectively). In all drying methods, naringin (41.04–43.76%), chloroginic acid (23.13–27.19%) and rosmarinic acid (12.26–15.95%) were the predominant phenol compounds. Although shade drying increased the antioxidant activity, aniseed extracts exhibited higher radical scavenging (IC50 = 10...
The intention of this study was to compare the different parts of Tunisian Ferula communis via th... more The intention of this study was to compare the different parts of Tunisian Ferula communis via their fatty acids, essential oils and phenolic compounds. Results showed that the lipid fraction of fruits and leaves was characterized by the predominance of oleic acid. Erucic and linoleic acids were the most abundant in stems while linoleic and palmitic acids in flowers. F. communis essential oils were defined by four chemotypes, namely isoshyobunone/6-tert-butyl-4-methylcoumarin in stems; α-eudesmol/caryophyllene oxide in leaves; caryophyllene/myrcene in flowers and α-gurjunene/hexadecanoic acid in fruits. So, oxygenated sesquiterpenes and sesquiterpene hydrocarbons represented the major classes of stem essential oil. Monoterpene hydrocarbons were the predominant classes of leaves and flowers. Fruit essential oil was predominated by sesquiterpene hydrocarbons. Resorcinol and ferulic acid were the main phenolic compounds in flowers but chlorogenic and ferulic acides in leaves. Stems wer...
Opuntia spp.: Chemistry, Bioactivity and Industrial Applications
Journal of Medicinal Plants Studies
Journal of Essential Oil Bearing Plants
Brazilian Journal of Pharmaceutical Sciences
The present study investigates the effect of cinnamon (Cinnamomum zeylanicumon) powder supplement... more The present study investigates the effect of cinnamon (Cinnamomum zeylanicumon) powder supplementation on glucose levels, lipid profiles, and oxidative stress parameters in alloxan-induced diabetic rats. Diabetes was induced in adult male Wistar rats via a single subcutaneous alloxan injection (15 mg/kg). Cinnamon powder was mixed with the standard feed of the rats in an amount of 5% for 28 consecutive days. Serum concentrations of total cholesterol (TC) and triglycerides (TG) were assayed at the end of the experimental period in all investigated groups. Anti-oxidative enzymes such as glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) were sought in the serum and pancreas. Alloxan caused the fasting blood sugar level to increase. The administration of cinnamon blocked the increase of blood glucose. There was also a significant difference in the TG and TC levels between control and treated diabetic rats. In diabetic rats, cinnamon treatment restored the activities of SOD, CAT and GPx. These findings suggested that cinnamon has an anti-hyperglycemic effect, improves lipid profiles, and protect against damage induced by oxidative stress in the diabetic state.
Brazilian Journal of Pharmaceutical Sciences
The purpose of the present investigation is to assess, for the first time, the antidiabetic, anti... more The purpose of the present investigation is to assess, for the first time, the antidiabetic, antihyperlipidemic and antioxidant activities of Lycium europaeum extract in alloxan-induced diabetic rats. Diabetes was induced in adult male Wistar rats via a single subcutaneous alloxan injection (120 mg/kg). Lycium europaeum aqueous extract was orally administered at a dose of 20 mg/kg for 28 consecutive days. Serum concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG) were assayed at the end of the experimental period in all investigated groups. Antioxidant enzymes such as glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) were sought in the serum and pancreas. Lycium europaeum extract significantly increased HDL-C and reduced blood glucose, TC, LDL-C and TG as compared to the alloxan-control group. Lycium europaeum extract was also efficient in reducing oxidative stress in diabetic rats by increasing SOD, CAT and GPx activities both in the pancreas and the plasma of the animals. Moreover, Lycium europaeum extract contained considerable levels of polyphenols and flavonoids. It also exhibited an important antioxidant capacity and a remarkable ability to quench DPPH radicals and reduce irons. The obtained results highlight potentially relevant health beneficial effects of Lycium europaeum extract, reversing hyperglycemic, hyperlipidemic and oxidative stress effects in rats with alloxan-induced diabetes. Therefore, it may be considered as a promising alternative or complementary agent to diabetes treatment.
Journal of Food Measurement and Characterization
The efect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of... more The efect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of their essential oils, phenolics and antioxidant activities. The optimum yield of essential oil was found in shade drying (2.62%). Fourteen volatile compounds were determined in all samples with variation of the main component proportions depending on drying methods. Thus, trans-anethole (84.21%) and estragole (3.82%) proportions signiicantly increased in shade drying. The highest total phenol and lavonoid contents of aniseeds were recorded in shade drying (42.70 mg of GAE/g and 53.55 mg of QE/g, respectively) while the lowest contents in oven drying at 60 °C (31.15 mg of GAE/g and 46.20 mg of QE/g, respectively). In all drying methods, naringin (41.04-43.76%), chloroginic acid (23.13-27.19%) and rosmarinic acid (12.26-15.95%) were the predominant phenol compounds. Although shade drying increased the antioxidant activity, aniseed extracts exhibited higher radical scavenging (IC 50 = 10.15 µg/mL), reducing power (EC 50 = 187.24 µg/mL) and chelating (IC 50 = 6.85 mg/mL) capacities than essential oils (IC 50 = 114.87 µg/mL, EC 50 = 548.05 µg/mL and IC 50 = 58.65 mg/mL, respectively). In conclusion, Shade drying method was found to enhance essential oils, phenols and antioxidant activities in aniseeds.
Industrial Crops and Products
Food and Bioprocess Technology
Journal of Food Biochemistry
Journal of Essential Oil Research
Food Science and Biotechnology
Journal of Food Biochemistry
Journal of exploratory research in pharmacology, Mar 11, 2022
Background and objectives: Rosemary (Rosmarinus officinalis L.) extract is widely used in food, c... more Background and objectives: Rosemary (Rosmarinus officinalis L.) extract is widely used in food, cosmetic and pharmaceutical industries. This study centered on the optimization of ethanolic extraction of rosemary leaves and evaluated the bioactivity of the extract. Methods: The optimized rosemary extract was analyzed using high-performance liquid chromatography. The antioxidant activity of the rosemary extract was measured using 2,2′-Azinobis-(3-ethylBenzoThiazoline-6-Sulfonic) acid, 2,2-DiPhenyl-1-Picryl-Hydrazyl-hydrate, and ferric reducing antioxidant power assays. The antibacterial activity of the rosemary extract was tested using the disk diffusion method. Toxicity was tested using mice. Nitrite production by RAW 264.7 cells was used to determine the anti-inflammatory activity of the rosemary extract. The construction and statistical analysis of the experimental design was done using NemrodW (LPRAI, version 2000) software. Results: We found that ethanol extraction of rosemary leaves depended on optimizing heating temperature and time. The maximum yields of carnosic and rosmarinic acids were obtained after optimizing these parameters according to the modelling software. The rosemary leaf extract had high antioxidant, anti-inflammatory, and antibacterial properties with low acute toxicity. Conclusions: Ethanol extraction of rosemary leaves could potentially be used in several industrial applications.
Le myrte (Myrtus communis L.), arbrisseau perenne spontane de la famille des Myrtacees, est tres ... more Le myrte (Myrtus communis L.), arbrisseau perenne spontane de la famille des Myrtacees, est tres connu par ses nombreuses vertus medicinales, nutritionnelles et ses nombreuses utilisations culinaires ce qui intensifie sa demande par les industries agroalimentaire et pharmaceutique. L’existence de deux varietes de myrte (Myrtus communis italica L. et Myrtus communis baetica L.) dans la flore de la Tunisie nous a incite a realiser ce travail de recherche afin d'evaluer biochimiquement la richesse des differents organes de ces deux varietes en composes bioactifs tels que les huiles essentielles, les acides gras et les polyphenols et de determiner leur activite antioxydante.
Journal of Food Biochemistry, 2010
Journal of Complementary and Integrative Medicine
Cancer is a major public health problem in the world. The use of the medicinal plants in cancer p... more Cancer is a major public health problem in the world. The use of the medicinal plants in cancer prevention and management is frequent in Africa, especially in Tunisia, and it is transmitted from generation to generation within cultures. Many previous studies showed that a wide range of Tunisian medicinal plants exerted cytotoxic and anticancer activity. A comprehensive review was conducted to collect information from scientific journal articles, including indigenous knowledge researches, about Tunisian medicinal plants used for the prevention and management of cancer. The aim of this review article is to provide the reader with information concerning the importance of Tunisian medicinal plants in the prevention and management of cancer and to open the door for the health professionals and scientists working in the field of pharmacology and therapeutics to produce new drug formulations to treat different types of cancer.
The effect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms o... more The effect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of their essential oils, phenolics and antioxidant activities. The optimum yield of essential oil was found in shade drying (2.62%). Fourteen volatile compounds were determined in all samples with variation of the main component proportions depending on drying methods. Thus, trans-anethole (84.21%) and estragole (3.82%) proportions significantly increased in shade drying. The highest total phenol and flavonoid contents of aniseeds were recorded in shade drying (42.70 mg of GAE/g and 53.55 mg of QE/g, respectively) while the lowest contents in oven drying at 60 °C (31.15 mg of GAE/g and 46.20 mg of QE/g, respectively). In all drying methods, naringin (41.04–43.76%), chloroginic acid (23.13–27.19%) and rosmarinic acid (12.26–15.95%) were the predominant phenol compounds. Although shade drying increased the antioxidant activity, aniseed extracts exhibited higher radical scavenging (IC50 = 10...
The intention of this study was to compare the different parts of Tunisian Ferula communis via th... more The intention of this study was to compare the different parts of Tunisian Ferula communis via their fatty acids, essential oils and phenolic compounds. Results showed that the lipid fraction of fruits and leaves was characterized by the predominance of oleic acid. Erucic and linoleic acids were the most abundant in stems while linoleic and palmitic acids in flowers. F. communis essential oils were defined by four chemotypes, namely isoshyobunone/6-tert-butyl-4-methylcoumarin in stems; α-eudesmol/caryophyllene oxide in leaves; caryophyllene/myrcene in flowers and α-gurjunene/hexadecanoic acid in fruits. So, oxygenated sesquiterpenes and sesquiterpene hydrocarbons represented the major classes of stem essential oil. Monoterpene hydrocarbons were the predominant classes of leaves and flowers. Fruit essential oil was predominated by sesquiterpene hydrocarbons. Resorcinol and ferulic acid were the main phenolic compounds in flowers but chlorogenic and ferulic acides in leaves. Stems wer...
Opuntia spp.: Chemistry, Bioactivity and Industrial Applications
Journal of Medicinal Plants Studies
Journal of Essential Oil Bearing Plants
Brazilian Journal of Pharmaceutical Sciences
The present study investigates the effect of cinnamon (Cinnamomum zeylanicumon) powder supplement... more The present study investigates the effect of cinnamon (Cinnamomum zeylanicumon) powder supplementation on glucose levels, lipid profiles, and oxidative stress parameters in alloxan-induced diabetic rats. Diabetes was induced in adult male Wistar rats via a single subcutaneous alloxan injection (15 mg/kg). Cinnamon powder was mixed with the standard feed of the rats in an amount of 5% for 28 consecutive days. Serum concentrations of total cholesterol (TC) and triglycerides (TG) were assayed at the end of the experimental period in all investigated groups. Anti-oxidative enzymes such as glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) were sought in the serum and pancreas. Alloxan caused the fasting blood sugar level to increase. The administration of cinnamon blocked the increase of blood glucose. There was also a significant difference in the TG and TC levels between control and treated diabetic rats. In diabetic rats, cinnamon treatment restored the activities of SOD, CAT and GPx. These findings suggested that cinnamon has an anti-hyperglycemic effect, improves lipid profiles, and protect against damage induced by oxidative stress in the diabetic state.
Brazilian Journal of Pharmaceutical Sciences
The purpose of the present investigation is to assess, for the first time, the antidiabetic, anti... more The purpose of the present investigation is to assess, for the first time, the antidiabetic, antihyperlipidemic and antioxidant activities of Lycium europaeum extract in alloxan-induced diabetic rats. Diabetes was induced in adult male Wistar rats via a single subcutaneous alloxan injection (120 mg/kg). Lycium europaeum aqueous extract was orally administered at a dose of 20 mg/kg for 28 consecutive days. Serum concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG) were assayed at the end of the experimental period in all investigated groups. Antioxidant enzymes such as glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) were sought in the serum and pancreas. Lycium europaeum extract significantly increased HDL-C and reduced blood glucose, TC, LDL-C and TG as compared to the alloxan-control group. Lycium europaeum extract was also efficient in reducing oxidative stress in diabetic rats by increasing SOD, CAT and GPx activities both in the pancreas and the plasma of the animals. Moreover, Lycium europaeum extract contained considerable levels of polyphenols and flavonoids. It also exhibited an important antioxidant capacity and a remarkable ability to quench DPPH radicals and reduce irons. The obtained results highlight potentially relevant health beneficial effects of Lycium europaeum extract, reversing hyperglycemic, hyperlipidemic and oxidative stress effects in rats with alloxan-induced diabetes. Therefore, it may be considered as a promising alternative or complementary agent to diabetes treatment.
Journal of Food Measurement and Characterization
The efect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of... more The efect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of their essential oils, phenolics and antioxidant activities. The optimum yield of essential oil was found in shade drying (2.62%). Fourteen volatile compounds were determined in all samples with variation of the main component proportions depending on drying methods. Thus, trans-anethole (84.21%) and estragole (3.82%) proportions signiicantly increased in shade drying. The highest total phenol and lavonoid contents of aniseeds were recorded in shade drying (42.70 mg of GAE/g and 53.55 mg of QE/g, respectively) while the lowest contents in oven drying at 60 °C (31.15 mg of GAE/g and 46.20 mg of QE/g, respectively). In all drying methods, naringin (41.04-43.76%), chloroginic acid (23.13-27.19%) and rosmarinic acid (12.26-15.95%) were the predominant phenol compounds. Although shade drying increased the antioxidant activity, aniseed extracts exhibited higher radical scavenging (IC 50 = 10.15 µg/mL), reducing power (EC 50 = 187.24 µg/mL) and chelating (IC 50 = 6.85 mg/mL) capacities than essential oils (IC 50 = 114.87 µg/mL, EC 50 = 548.05 µg/mL and IC 50 = 58.65 mg/mL, respectively). In conclusion, Shade drying method was found to enhance essential oils, phenols and antioxidant activities in aniseeds.
Industrial Crops and Products
Food and Bioprocess Technology
Journal of Food Biochemistry
Journal of Essential Oil Research
Food Science and Biotechnology
Journal of Food Biochemistry