Y. Henin - Academia.edu (original) (raw)
Papers by Y. Henin
ChemInform, 1997
Preparation and anti-HIV Activity of N-3-Substituted Thymidine Nucleoside Analogues. -Twenty-two ... more Preparation and anti-HIV Activity of N-3-Substituted Thymidine Nucleoside Analogues. -Twenty-two new compounds, e.g. (I), are evaluated for anti-HIV activity. None of them displays significant inhibition of HIV reverse transcriptase, indicating phosphorylation as necessary prerequisite for activity. -(ADAMS, D. R.; PEREZ, C.; MAILLARD, M.; FLORENT, J.-C.; EVERS, M.; HENIN, Y.; LITVAK, S.; LITVAK, L.; MONNERET, C.; GRIERSON, D. S.; J. Med. Chem. 40 (1997) 10, 1550-1558; Inst. Chim. Subst. Nat., CNRS, F-91198 Gif-sur-Yvette, Fr.; EN)
Médecine et Maladies Infectieuses, 1993
La Revue de médecine interne / fondée ... par la Société nationale francaise de médecine interne, 2008
The human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome induce account ... more The human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome induce account for over 40 million deaths in the past 20 years. Given that the currently available treatments to prevent HIV transmission and disease are not effective in eradicating the virus, vaccination likely represents the only efficacious adapted response to the global impact of this infection. This paper reviews the challenges encountered in the development of an HIV vaccine as well as the different vaccine approaches and main HIV vaccine candidates evaluated in clinical trials. In spite the tremendous progress in HIV research, the major challenges that are encountered in the development of an HIV vaccine remain of scientific order and include viral specificities, absence of correlates of immune protection and limitations of existing animal models. Over 30 vaccine candidates have been evaluated in clinical trials. These vaccine approaches include the use of recombinant envelope proteins, DNA vac...
Research in virology
Tetracycline analogs were evaluated for anti-HIV activity in CEM cells; minocycline and doxycycli... more Tetracycline analogs were evaluated for anti-HIV activity in CEM cells; minocycline and doxycycline were the most active of these in inhibiting the virus-induced cytopathic effect between 7 and 14 days post-infection. The active concentrations (0.3-1.5 micrograms/ml) were devoid of toxicity in uninfected cultures. Virus production, however, was not inhibited, indicating a dissociation between protection against cell death and suppression of virus growth. These protected cells could be maintained in culture for 6-7 weeks, even in the absence of the compounds. After that period, virus production ceased and cells could then be cultivated for several months without loss of viability or reappearance of virus production. As HIV stocks produced in the presence of tetracycline analogs were unable to induce cell death, we suggest that the cytopathogenicity of HIV may be due in some cases to the presence of tetracycline-sensitive contaminating microorganisms.
Journal of medicinal chemistry, 1991
Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-ma... more Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-mannopyranoside) and a hexadecyl chain were prepared from glucose 6-phosphate and D-mannose precursors. The 31P NMR study of the mannosyl phosphotriester series in the presence of large unilamellar vesicles demonstrated either an interaction with the external lipid layer or a transmembrane transport into the intravesicular interface. The antiviral activity, measured by the inhibition of cytopathogenicity on different infected cells and of reverse transcriptase activity in the supernatant of cultures, appeared to be comparable to that of AZT, in the micromolar range.
ChemInform, 1993
ABSTRACT The syntheses of two O-alkyl-5′,5′-dinucleoside phosphotriesters 2a and 2b as combined p... more ABSTRACT The syntheses of two O-alkyl-5′,5′-dinucleoside phosphotriesters 2a and 2b as combined prodrugs of the antiviral drug AZT (1) and the antibiotic agent cordycepin (3) are described. 2a,b were obtained as a 1:1 diastereomeric mixture. The absolute configuration of the isolated diastereomers was determined by NOE NMR experiments and correlated with the migration characteristics on silica gel as well as the 31P-NMR chemical shift. The conformational features of 2bR and 2bS were determined in deuteriated dodecylphosphocholine micelles in aqueous solution using 2D-NOESY spectra and shown to be dependent on the configuration at phosphorus. Additionally, all new compounds were tested for their antiviral activities in HIV-1-infected CEM C113 and H9 cell systems. Although all compounds were able to significantly inhibit the HIV-1-induced cytopathogenic effect, only the phosphodiester 12 gave a selectivity index (SIRT = 2000) comparable to the reference compound AZT (1) (SIRT = 3000).
Nucleosides, Nucleotides and Nucleic Acids, 2000
The synthesis of 3'-O2-(azaheterocycle)-thymidines is presented from 1-thia-3-aza- 1,3-bu... more The synthesis of 3'-O2-(azaheterocycle)-thymidines is presented from 1-thia-3-aza- 1,3-butadiene precursors (N-thioacylamidines). A variety of heterocycles is accessible using the dienic, the electrophilic or the nucleophilic reactivity of these thia-azabutadiene systems. 3'-O2-(azaheterocycle)-thymidine analogues are regarded as potential substrates to interfere with the DNA-polymerization process.
The Journal of Organic Chemistry, 1996
Graziosi, C.; Demarest, J. F.; Cohen, O. J.; Vaccarezza, M.; Gantt, K.; Muro-Cacho, C.; Fauci, A.... more Graziosi, C.; Demarest, J. F.; Cohen, O. J.; Vaccarezza, M.; Gantt, K.; Muro-Cacho, C.; Fauci, A. S. Immunol. Rev. 1994,140, 105. Emilie, D.; Fior, R.; Llorente, L.; Marfaing-Kora, A.; Peuchmaur, M.; Devergne, O.; Jarrousse, B.; Wudenes, J.; Boue, F.; Galanaud, P. Immunol. Rev. 1994, 140, 5. Wei, X.; Ghosh, S. K.; Taylor, M. E.; Johnson, V. A.; Emini, E. A.; Deutsch, P.; Lifson, J. D.; Bonhoeffer, S.; Nowak, M. A.; Han, B. H.; Saag, M. S.; Shaw, G. M. Nature 1995, 373, 117. Ho, D. D.; Neumann, A. U.; Perleson, A. S.; Chen, W.; Leonars, J. M.; Markowitz, M. Nature 1995, 373, 123. (3) Huynh Dinh, T. Curr. Opin. Invest. Drugs 1993, 2, 905. (4) Furman, P. A.; Fyfe, J. A.; St. Clair, M. H.; Weinhold, K.; Rideout, J. L.; Freeman, G.A.; Lehrman, S. N.; Bolognesi, D. P.; Broder, S.; Mitsuya, H.; Barry, D. W. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 8333. (5) Izuta, S.; Saneyoshi, M.; Sakurai, T.; Suzuki, M.; Kojima, K.; Yosida, S. Biochem. Biophys. Res. Commun. 1991, 179, 776. (6) Li, T.; Krasne, S. J.; Persson, B.; Kaback, R.; Dietrich, F. J. Org. Chem. 1993, 58, 380. (7) Bonnaffé, D.; Dupraz, B.; Ughetto-Monfrin, J.; Namane, A.; Huynh Dinh, T. Tetrahedron Lett. 1995, 36, 531. (8) Hydrolysis of acetyl phosphate into acetyl and phosphate requires 3-6 kcal less than that of ATP to ADP (see: Biol. Chem.; Mahler, H. R., Cordes, E. H., Eds.; Harper Int.: New York, 1963; p 201).
Journal of Medicinal Chemistry, 1991
Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-ma... more Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-mannopyranoside) and a hexadecyl chain were prepared from glucose 6-phosphate and D-mannose precursors. The 31P NMR study of the mannosyl phosphotriester series in the presence of large unilamellar vesicles demonstrated either an interaction with the external lipid layer or a transmembrane transport into the intravesicular interface. The antiviral activity, measured by the inhibition of cytopathogenicity on different infected cells and of reverse transcriptase activity in the supernatant of cultures, appeared to be comparable to that of AZT, in the micromolar range.
British journal of cancer, 1979
The cytolysis of 3H-proline-labelled tumour cells growing in monolayer by syngeneic immune lympho... more The cytolysis of 3H-proline-labelled tumour cells growing in monolayer by syngeneic immune lymphocytes has been studied in the murine sarcoma virus (MSV) system. Results show that the proline assay (PA) is a convenient way to reveal the activity of cytolytic T lymphocytes against FMR-like antigens. Using the same effector and target cells, the classical chromium-release test (CRT) fails to reveal any cytolytic activity, and the visual microcytotoxicity assay as well as several derived isotopic methods are known to reveal mainly non-specific reactions due to non-T effector cells. The PA, therefore, appears to be a useful method for testing an antitumour reaction against tumour cells in monolayer. The results are, however, different from those obtained in the CRT using the same effector cells but lymphoma cells in suspension as targets, the major discrepancies being the following: (a) the PA does not provide truly quantitative data, due to the very high lymphoid: effector cell ratios ...
Research in virology
Human monocyte-derived macrophages (MDM) were infected with the viral strain HIV1/Ba-L and with t... more Human monocyte-derived macrophages (MDM) were infected with the viral strain HIV1/Ba-L and with the clinical isolates HIV1/DAS and HIV1/PAR. Kinetics of tumour necrosis factor alpha (TNF alpha) and interleukin-6 (IL6) production were investigated for 28 days after infection. At the early stages of infection we observed significant TNF alpha and IL6 secretion 2 to 10 h after infection, whatever the viral strain we used. During the late events of MDM infection, TNF alpha and IL6 were detected over 16 to 21 days following HIV1 infection, at the time of high viral replication. Pretreatment of MDM with a TNF alpha synthesis inhibitor, RP 55778, 4 h prior to HIV infection induced a modified cytokine pattern during the first ten hours of infection: TNF alpha production was totally inhibited despite comparable amounts of IL6. At the late phases of the cell culture, a decrease in magnitude of both viral and cytokine production as well as a delay in the appearance of reverse transcriptase act...
Journal of immunology (Baltimore, Md. : 1950), 1983
L1-16, a DR-specific, nonpolymorphic monoclonal antibody (MoAb) recognizing the beta-chain of the... more L1-16, a DR-specific, nonpolymorphic monoclonal antibody (MoAb) recognizing the beta-chain of the molecule, blocked virus-specific as well as allogeneic-proliferative T cell responses, whereas another nonpolymorphic anti-DR MoAb blocked proliferative responses to allogeneic cells only. IL 2-supernatants reversed the blocking induced by L1-16. On the other hand class I-specific MoAb B1-23-2 (HLA-A-B-C-specific) and M 18 (beta 2 microglobulin-specific) and their F(ab')2 fragments blocked equally virus-specific or allogeneic proliferative responses. IL 2-containing supernatants did not reverse this phenomenon. Neither anti-class I nor anti-class II MoAb inhibited the IL 2-induced proliferation of IL 2-dependent established T cell lines. MoAb acted neither at the antigen-presenting cell level nor by a toxic effect nor by inducing suppressor cells. These results suggest that a) different epitopes of the DR molecule can be involved during responses to influenza viruses and allogeneic ...
Immunogenetics, 1986
We studied the polymorphisms of HLA-DR and HLA-DQ products from HLA-DRw13 haplotypes by analyzing... more We studied the polymorphisms of HLA-DR and HLA-DQ products from HLA-DRw13 haplotypes by analyzing the restriction of influenza A-specific cloned T cells from an HLA-DRw13,DQw1,Dw19 homozygous individual. The results show that some functional epitopes, which can be borne by either HLA-DR or HLA-DQ molecules, are strictly correlated with the HLA-Dw19 subtype of HLA-DRw13. This clearly indicates that both HLA-DR and HLA-DQ products contribute to the HLA-Dw19 subdivision of HLA-DRw13. At least two different restricting epitopes are borne by DR products: one is correlated with the HLA-DRw13 serologically defined specificity, which includes Dw19 and Dw18 haplotypes; the other is correlated with the only HLA-Dw19 subtype of HLA-DRw13. Restricting epitopes borne by DQ molecules have been found on Dw19 cells only. DQ-restricted clones were unable to react with DQw1 APC of any other haplotypes tested, including DR1, DR2-long, DR2-short, and DRw14, demonstrating a high degree of functional pol...
AIDS research and human retroviruses, 1991
Page 1. AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 7, Number 1, 1991 Mary Ann Liebert, Inc., Pub... more Page 1. AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 7, Number 1, 1991 Mary Ann Liebert, Inc., Publishers Short Communication Is There a Neutralization Epitope in the Second Conserved Domain of HIV-1 Envelope Protein? ...
Journal of virology, 1992
Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1)... more Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1) strain LAI or RF have been expressed in antigenic site 1 of the capsid of the Sabin strain of poliovirus type 1. A number of the resulting chimeras were viable. Viable variants bearing mutations within the insertion site spontaneously arose from several nonviable chimeras. In general, these mutations result in a decrease in positive charge in the substituted antigenic site 1. Two of the chimeras were genetically stable and have been further characterized. Both chimeras were neutralized by various HIV-1 neutralizing antibodies. In rabbits, both chimeras produced high levels of antibodies which react with HIV-1 gp120/160 in immunoprecipitation and enzyme-linked immunosorbent assays. One of the chimeras (HIV-1LAI) produced a significant but weak HIV-1 neutralizing response.
Journal of immunogenetics, 1977
Cytolytic T lymphocytes (CTL) from murine sarcoma virus (MSV) or Friend leukaemia virus (FLV) ino... more Cytolytic T lymphocytes (CTL) from murine sarcoma virus (MSV) or Friend leukaemia virus (FLV) inoculated mice lyse syngeneic much more efficiently than allogeneic FMRGi+ lymphoma cells. By comparing the cytolysis of various H-2 different 51Cr lymphomas by CTL from several inbred and congenic lines differing at H-2, and by competition experiments using unlabelled cells, one can demonstrate that this phenomenon is due to an H-2 barrier. H-2b/H-2d hybrid-anti-MSV-CTL immunized by H-2b, H-2d or H-2b/H-2d tumours lyse only FMRGi+ lymphomas of the same H-2, and their activity for a given target is inhibited only by H-2-identical competitive cells. H-2 antigens are therefore directly involved in the interaction between tumour cells and immune CTL which probably react with an 'H-2 modified' antigen of the tumour cells surface. The use of CTL from intra-H-2 recombinant lines shows that H-2D and probably H-2K molecules are involved, but vary according to the tumour cells. A possible r...
Journal of Biological Chemistry, 2000
The MAPK pathway is required for T-cell activation; however, its role in modulating T-cell functi... more The MAPK pathway is required for T-cell activation; however, its role in modulating T-cell function following human immunodeficiency virus type 1 (HIV-1) infection is poorly understood. In this report, we investigated whether Grb3-3, an isoform of the Grb2 (growth ...
Revue Française de Transfusion et d'Hémobiologie, 1990
ABSTRACT
ChemInform, 1997
Preparation and anti-HIV Activity of N-3-Substituted Thymidine Nucleoside Analogues. -Twenty-two ... more Preparation and anti-HIV Activity of N-3-Substituted Thymidine Nucleoside Analogues. -Twenty-two new compounds, e.g. (I), are evaluated for anti-HIV activity. None of them displays significant inhibition of HIV reverse transcriptase, indicating phosphorylation as necessary prerequisite for activity. -(ADAMS, D. R.; PEREZ, C.; MAILLARD, M.; FLORENT, J.-C.; EVERS, M.; HENIN, Y.; LITVAK, S.; LITVAK, L.; MONNERET, C.; GRIERSON, D. S.; J. Med. Chem. 40 (1997) 10, 1550-1558; Inst. Chim. Subst. Nat., CNRS, F-91198 Gif-sur-Yvette, Fr.; EN)
Médecine et Maladies Infectieuses, 1993
La Revue de médecine interne / fondée ... par la Société nationale francaise de médecine interne, 2008
The human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome induce account ... more The human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome induce account for over 40 million deaths in the past 20 years. Given that the currently available treatments to prevent HIV transmission and disease are not effective in eradicating the virus, vaccination likely represents the only efficacious adapted response to the global impact of this infection. This paper reviews the challenges encountered in the development of an HIV vaccine as well as the different vaccine approaches and main HIV vaccine candidates evaluated in clinical trials. In spite the tremendous progress in HIV research, the major challenges that are encountered in the development of an HIV vaccine remain of scientific order and include viral specificities, absence of correlates of immune protection and limitations of existing animal models. Over 30 vaccine candidates have been evaluated in clinical trials. These vaccine approaches include the use of recombinant envelope proteins, DNA vac...
Research in virology
Tetracycline analogs were evaluated for anti-HIV activity in CEM cells; minocycline and doxycycli... more Tetracycline analogs were evaluated for anti-HIV activity in CEM cells; minocycline and doxycycline were the most active of these in inhibiting the virus-induced cytopathic effect between 7 and 14 days post-infection. The active concentrations (0.3-1.5 micrograms/ml) were devoid of toxicity in uninfected cultures. Virus production, however, was not inhibited, indicating a dissociation between protection against cell death and suppression of virus growth. These protected cells could be maintained in culture for 6-7 weeks, even in the absence of the compounds. After that period, virus production ceased and cells could then be cultivated for several months without loss of viability or reappearance of virus production. As HIV stocks produced in the presence of tetracycline analogs were unable to induce cell death, we suggest that the cytopathogenicity of HIV may be due in some cases to the presence of tetracycline-sensitive contaminating microorganisms.
Journal of medicinal chemistry, 1991
Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-ma... more Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-mannopyranoside) and a hexadecyl chain were prepared from glucose 6-phosphate and D-mannose precursors. The 31P NMR study of the mannosyl phosphotriester series in the presence of large unilamellar vesicles demonstrated either an interaction with the external lipid layer or a transmembrane transport into the intravesicular interface. The antiviral activity, measured by the inhibition of cytopathogenicity on different infected cells and of reverse transcriptase activity in the supernatant of cultures, appeared to be comparable to that of AZT, in the micromolar range.
ChemInform, 1993
ABSTRACT The syntheses of two O-alkyl-5′,5′-dinucleoside phosphotriesters 2a and 2b as combined p... more ABSTRACT The syntheses of two O-alkyl-5′,5′-dinucleoside phosphotriesters 2a and 2b as combined prodrugs of the antiviral drug AZT (1) and the antibiotic agent cordycepin (3) are described. 2a,b were obtained as a 1:1 diastereomeric mixture. The absolute configuration of the isolated diastereomers was determined by NOE NMR experiments and correlated with the migration characteristics on silica gel as well as the 31P-NMR chemical shift. The conformational features of 2bR and 2bS were determined in deuteriated dodecylphosphocholine micelles in aqueous solution using 2D-NOESY spectra and shown to be dependent on the configuration at phosphorus. Additionally, all new compounds were tested for their antiviral activities in HIV-1-infected CEM C113 and H9 cell systems. Although all compounds were able to significantly inhibit the HIV-1-induced cytopathogenic effect, only the phosphodiester 12 gave a selectivity index (SIRT = 2000) comparable to the reference compound AZT (1) (SIRT = 3000).
Nucleosides, Nucleotides and Nucleic Acids, 2000
The synthesis of 3'-O2-(azaheterocycle)-thymidines is presented from 1-thia-3-aza- 1,3-bu... more The synthesis of 3'-O2-(azaheterocycle)-thymidines is presented from 1-thia-3-aza- 1,3-butadiene precursors (N-thioacylamidines). A variety of heterocycles is accessible using the dienic, the electrophilic or the nucleophilic reactivity of these thia-azabutadiene systems. 3'-O2-(azaheterocycle)-thymidine analogues are regarded as potential substrates to interfere with the DNA-polymerization process.
The Journal of Organic Chemistry, 1996
Graziosi, C.; Demarest, J. F.; Cohen, O. J.; Vaccarezza, M.; Gantt, K.; Muro-Cacho, C.; Fauci, A.... more Graziosi, C.; Demarest, J. F.; Cohen, O. J.; Vaccarezza, M.; Gantt, K.; Muro-Cacho, C.; Fauci, A. S. Immunol. Rev. 1994,140, 105. Emilie, D.; Fior, R.; Llorente, L.; Marfaing-Kora, A.; Peuchmaur, M.; Devergne, O.; Jarrousse, B.; Wudenes, J.; Boue, F.; Galanaud, P. Immunol. Rev. 1994, 140, 5. Wei, X.; Ghosh, S. K.; Taylor, M. E.; Johnson, V. A.; Emini, E. A.; Deutsch, P.; Lifson, J. D.; Bonhoeffer, S.; Nowak, M. A.; Han, B. H.; Saag, M. S.; Shaw, G. M. Nature 1995, 373, 117. Ho, D. D.; Neumann, A. U.; Perleson, A. S.; Chen, W.; Leonars, J. M.; Markowitz, M. Nature 1995, 373, 123. (3) Huynh Dinh, T. Curr. Opin. Invest. Drugs 1993, 2, 905. (4) Furman, P. A.; Fyfe, J. A.; St. Clair, M. H.; Weinhold, K.; Rideout, J. L.; Freeman, G.A.; Lehrman, S. N.; Bolognesi, D. P.; Broder, S.; Mitsuya, H.; Barry, D. W. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 8333. (5) Izuta, S.; Saneyoshi, M.; Sakurai, T.; Suzuki, M.; Kojima, K.; Yosida, S. Biochem. Biophys. Res. Commun. 1991, 179, 776. (6) Li, T.; Krasne, S. J.; Persson, B.; Kaback, R.; Dietrich, F. J. Org. Chem. 1993, 58, 380. (7) Bonnaffé, D.; Dupraz, B.; Ughetto-Monfrin, J.; Namane, A.; Huynh Dinh, T. Tetrahedron Lett. 1995, 36, 531. (8) Hydrolysis of acetyl phosphate into acetyl and phosphate requires 3-6 kcal less than that of ATP to ADP (see: Biol. Chem.; Mahler, H. R., Cordes, E. H., Eds.; Harper Int.: New York, 1963; p 201).
Journal of Medicinal Chemistry, 1991
Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-ma... more Phosphate derivatives of AZT esterified with a carbohydrate (D-glucose, D-mannose, and ethyl D-mannopyranoside) and a hexadecyl chain were prepared from glucose 6-phosphate and D-mannose precursors. The 31P NMR study of the mannosyl phosphotriester series in the presence of large unilamellar vesicles demonstrated either an interaction with the external lipid layer or a transmembrane transport into the intravesicular interface. The antiviral activity, measured by the inhibition of cytopathogenicity on different infected cells and of reverse transcriptase activity in the supernatant of cultures, appeared to be comparable to that of AZT, in the micromolar range.
British journal of cancer, 1979
The cytolysis of 3H-proline-labelled tumour cells growing in monolayer by syngeneic immune lympho... more The cytolysis of 3H-proline-labelled tumour cells growing in monolayer by syngeneic immune lymphocytes has been studied in the murine sarcoma virus (MSV) system. Results show that the proline assay (PA) is a convenient way to reveal the activity of cytolytic T lymphocytes against FMR-like antigens. Using the same effector and target cells, the classical chromium-release test (CRT) fails to reveal any cytolytic activity, and the visual microcytotoxicity assay as well as several derived isotopic methods are known to reveal mainly non-specific reactions due to non-T effector cells. The PA, therefore, appears to be a useful method for testing an antitumour reaction against tumour cells in monolayer. The results are, however, different from those obtained in the CRT using the same effector cells but lymphoma cells in suspension as targets, the major discrepancies being the following: (a) the PA does not provide truly quantitative data, due to the very high lymphoid: effector cell ratios ...
Research in virology
Human monocyte-derived macrophages (MDM) were infected with the viral strain HIV1/Ba-L and with t... more Human monocyte-derived macrophages (MDM) were infected with the viral strain HIV1/Ba-L and with the clinical isolates HIV1/DAS and HIV1/PAR. Kinetics of tumour necrosis factor alpha (TNF alpha) and interleukin-6 (IL6) production were investigated for 28 days after infection. At the early stages of infection we observed significant TNF alpha and IL6 secretion 2 to 10 h after infection, whatever the viral strain we used. During the late events of MDM infection, TNF alpha and IL6 were detected over 16 to 21 days following HIV1 infection, at the time of high viral replication. Pretreatment of MDM with a TNF alpha synthesis inhibitor, RP 55778, 4 h prior to HIV infection induced a modified cytokine pattern during the first ten hours of infection: TNF alpha production was totally inhibited despite comparable amounts of IL6. At the late phases of the cell culture, a decrease in magnitude of both viral and cytokine production as well as a delay in the appearance of reverse transcriptase act...
Journal of immunology (Baltimore, Md. : 1950), 1983
L1-16, a DR-specific, nonpolymorphic monoclonal antibody (MoAb) recognizing the beta-chain of the... more L1-16, a DR-specific, nonpolymorphic monoclonal antibody (MoAb) recognizing the beta-chain of the molecule, blocked virus-specific as well as allogeneic-proliferative T cell responses, whereas another nonpolymorphic anti-DR MoAb blocked proliferative responses to allogeneic cells only. IL 2-supernatants reversed the blocking induced by L1-16. On the other hand class I-specific MoAb B1-23-2 (HLA-A-B-C-specific) and M 18 (beta 2 microglobulin-specific) and their F(ab')2 fragments blocked equally virus-specific or allogeneic proliferative responses. IL 2-containing supernatants did not reverse this phenomenon. Neither anti-class I nor anti-class II MoAb inhibited the IL 2-induced proliferation of IL 2-dependent established T cell lines. MoAb acted neither at the antigen-presenting cell level nor by a toxic effect nor by inducing suppressor cells. These results suggest that a) different epitopes of the DR molecule can be involved during responses to influenza viruses and allogeneic ...
Immunogenetics, 1986
We studied the polymorphisms of HLA-DR and HLA-DQ products from HLA-DRw13 haplotypes by analyzing... more We studied the polymorphisms of HLA-DR and HLA-DQ products from HLA-DRw13 haplotypes by analyzing the restriction of influenza A-specific cloned T cells from an HLA-DRw13,DQw1,Dw19 homozygous individual. The results show that some functional epitopes, which can be borne by either HLA-DR or HLA-DQ molecules, are strictly correlated with the HLA-Dw19 subtype of HLA-DRw13. This clearly indicates that both HLA-DR and HLA-DQ products contribute to the HLA-Dw19 subdivision of HLA-DRw13. At least two different restricting epitopes are borne by DR products: one is correlated with the HLA-DRw13 serologically defined specificity, which includes Dw19 and Dw18 haplotypes; the other is correlated with the only HLA-Dw19 subtype of HLA-DRw13. Restricting epitopes borne by DQ molecules have been found on Dw19 cells only. DQ-restricted clones were unable to react with DQw1 APC of any other haplotypes tested, including DR1, DR2-long, DR2-short, and DRw14, demonstrating a high degree of functional pol...
AIDS research and human retroviruses, 1991
Page 1. AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 7, Number 1, 1991 Mary Ann Liebert, Inc., Pub... more Page 1. AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 7, Number 1, 1991 Mary Ann Liebert, Inc., Publishers Short Communication Is There a Neutralization Epitope in the Second Conserved Domain of HIV-1 Envelope Protein? ...
Journal of virology, 1992
Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1)... more Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1) strain LAI or RF have been expressed in antigenic site 1 of the capsid of the Sabin strain of poliovirus type 1. A number of the resulting chimeras were viable. Viable variants bearing mutations within the insertion site spontaneously arose from several nonviable chimeras. In general, these mutations result in a decrease in positive charge in the substituted antigenic site 1. Two of the chimeras were genetically stable and have been further characterized. Both chimeras were neutralized by various HIV-1 neutralizing antibodies. In rabbits, both chimeras produced high levels of antibodies which react with HIV-1 gp120/160 in immunoprecipitation and enzyme-linked immunosorbent assays. One of the chimeras (HIV-1LAI) produced a significant but weak HIV-1 neutralizing response.
Journal of immunogenetics, 1977
Cytolytic T lymphocytes (CTL) from murine sarcoma virus (MSV) or Friend leukaemia virus (FLV) ino... more Cytolytic T lymphocytes (CTL) from murine sarcoma virus (MSV) or Friend leukaemia virus (FLV) inoculated mice lyse syngeneic much more efficiently than allogeneic FMRGi+ lymphoma cells. By comparing the cytolysis of various H-2 different 51Cr lymphomas by CTL from several inbred and congenic lines differing at H-2, and by competition experiments using unlabelled cells, one can demonstrate that this phenomenon is due to an H-2 barrier. H-2b/H-2d hybrid-anti-MSV-CTL immunized by H-2b, H-2d or H-2b/H-2d tumours lyse only FMRGi+ lymphomas of the same H-2, and their activity for a given target is inhibited only by H-2-identical competitive cells. H-2 antigens are therefore directly involved in the interaction between tumour cells and immune CTL which probably react with an 'H-2 modified' antigen of the tumour cells surface. The use of CTL from intra-H-2 recombinant lines shows that H-2D and probably H-2K molecules are involved, but vary according to the tumour cells. A possible r...
Journal of Biological Chemistry, 2000
The MAPK pathway is required for T-cell activation; however, its role in modulating T-cell functi... more The MAPK pathway is required for T-cell activation; however, its role in modulating T-cell function following human immunodeficiency virus type 1 (HIV-1) infection is poorly understood. In this report, we investigated whether Grb3-3, an isoform of the Grb2 (growth ...
Revue Française de Transfusion et d'Hémobiologie, 1990
ABSTRACT