Y. Lo - Academia.edu (original) (raw)

Papers by Y. Lo

IEEE Photonics Technology Letters, 2002

Journal of gynecologic oncology, 2011

To compare the outcomes of total laparoscopic to robotic approach for hysterectomy and all indica... more To compare the outcomes of total laparoscopic to robotic approach for hysterectomy and all indicated procedures after controlling for surgeon and other confounding factors. Retrospective chart review of all consecutive cases of total laparoscopic and da Vinci robotic hysterectomies between August 2007 and July 2009 by two gynecologic oncology surgeons. Our primary outcome measure was operative procedure time. Secondary measures included complications, conversion to laparotomy, estimated blood loss and length of hospital stay. A mixed model with a random intercept was applied to control for surgeon and other confounders. Wilcoxon rank-sum, chi-square and Fisher's exact tests were used for the statistical analysis. The 124 patients included in the study consisted of 77 total laparoscopic hysterectomies and 47 robotic hysterectomies. Both groups had similar baseline characteristics, indications for surgery and additional procedures performed. The difference between the mean operati...

Journal of Minimally Invasive Gynecology, 2010

Patients: Twelve women with uterine disease requiring surgical management. Intervention: Transumb... more Patients: Twelve women with uterine disease requiring surgical management. Intervention: Transumbilical single incision total laparoscopic hysterectomy. Measurements and Main Results: Operation time, length of hospital stay and postoperative complications were assessed in this trial. Articulating and rigid instruments, 30 degrees 10 mm telescope, SILS port and advanced bipolar and mechanic energy modalities were used in this technique. In all cases vaginal cuff were sutured laparoscopically. Sutures were tied with extracorporeal technique. The mean age of patients and mean operative time were found 48 AE4 (ranged 42-55) years and 108AE29.4 (ranged 80-180) minutes, respectively. There were no intraoperative complications. All patients were discharged within postoperative 48 hours. The mean length of hospital stay and mean blood loss were found 1.6AE0.49 days and 45AE23.1 ml, respectively. Conclusion: Total laparoscopic hysterectomy performed through transumbilical single incision is technically feasible and safe. Development of advanced flexible instrumentation and visualization platform may facilitate this new operative approach.

Scientific reports, Jan 16, 2018

The coffee-ring effect, ubiquitously present in the drying process of aqueous droplets, impedes t... more The coffee-ring effect, ubiquitously present in the drying process of aqueous droplets, impedes the performance of a myriad of applications involving precipitation of particle suspensions in evaporating liquids on solid surfaces, such as liquid biopsy combinational analysis, microarray fabrication, and ink-jet printing, to name a few. We invented the methodology of laser-induced differential evaporation to remove the coffee-ring effect. Without any additives to the liquid or any morphology modifications of the solid surface the liquid rests on, we have eliminated the coffee-ring effect by engineering the liquid evaporation profile with a CO laser irradiating the apex of the droplets. The method of laser-induced differential evaporation transitions particle deposition patterns from coffee-ring patterns to central-peak patterns, bringing all particles (e.g. fluorescent double strand DNAs) in the droplet to a designated area of 100 μm diameter without leaving any stains outside. The te...

Blood, 2010

Constitutive JAK2 activation in hematopoietic cells by the JAK2V617F mutation recapitulates myelo... more Constitutive JAK2 activation in hematopoietic cells by the JAK2V617F mutation recapitulates myeloproliferative neoplasm (MPN) phenotypes in mice, establishing JAK2 inhibition as a potential therapeutic strategy. Although most polycythemia vera patients carry the JAK2V617F mutation, half of those with essential thrombocythemia or primary myelofibrosis do not, suggesting alternative mechanisms for constitutive JAK-STAT signaling in MPNs. Most patients with primary myelofibrosis have elevated levels of JAK-dependent proinflammatory cytokines (eg, interleukin-6) consistent with our observation of JAK1 hyperactivation. Accordingly, we evaluated the effectiveness of selective JAK1/2 inhibition in experimental models relevant to MPNs and report on the effects of INCB018424, the first potent, selective, oral JAK1/JAK2 inhibitor to enter the clinic. INCB018424 inhibited interleukin-6 signaling (50% inhibitory concentration [IC50] = 281nM), and proliferation of JAK2V617F+ Ba/F3 cells (IC50 = ...

Applied Physics Letters, 2012

BIO-PROTOCOL, 2017

To study the inhomogeneity within a cell population including exosomes properties such as exosome... more To study the inhomogeneity within a cell population including exosomes properties such as exosome secretion rate of cells and surface markers carried by exosomes, we need to quantify and characterize those exosomes secreted by each individual cell. Here we develop a method to collect and analyze exosomes secreted by an array of single cells using antibody-modified glass slides that are position-registered to each single cell. After each collection, antibody-conjugated quantum dots are used to label exosomes to allow counting and analysis of exosome surface proteins. Detailed studies of exosome properties related to cell behaviors such as responses to drugs and stress at single cell resolution can be found in the publication (Chiu et al., 2016).

Applied Physics Letters, 2007

An InGaAs single photon avalanche detector capable of sub-Geiger mode (Photomultiplier-tube-like)... more An InGaAs single photon avalanche detector capable of sub-Geiger mode (Photomultiplier-tube-like) operation is reported. The device achieves a stable gain at around . The gain fluctuation is greatly suppressed through a self-quenching effect, thus an ...

Annals of Biomedical Engineering, 2014

Detection of low abundance biomolecules is challenging for biosensors that rely on surface chemic... more Detection of low abundance biomolecules is challenging for biosensors that rely on surface chemical reactions. For surface reaction based biosensors, it require to take hours or even days for biomolecules of diffusivities in the order of 10(-10-11) m2/s to reach the surface of the sensors by Brownian motion. In addition, often times the repelling Coulomb interactions between the molecules and the probes further defer the binding process, leading to undesirably long detection time for applications such as point-of-care in vitro diagnosis. In this work, we designed an oil encapsulated nanodroplet array microchip utilizing evaporation for pre-concentration of the targets to greatly shorten the reaction time and enhance the detection sensitivity. The evaporation process of the droplets is facilitated by the superhydrophilic surface and resulting nanodroplets are encapsulated by oil drops to form stable reaction chamber. Using this method, desirable droplet volumes, concentrations of target molecules, and reaction conditions (salt concentrations, reaction temperature, etc.) in favour of fast and sensitive detection are obtained. A linear response over 2 orders of magnitude in target concentration was achieved at 10 fM for protein targets and 100 fM for miRNA mimic oligonucleotides.