Y. Yin - Academia.edu (original) (raw)

Papers by Y. Yin

Springer Handbook of Geographic Information, 2011

Vaccine, Jan 28, 2014

Dendritic cells (DCs), professional antigen presenting cells, have demonstrated effective in cont... more Dendritic cells (DCs), professional antigen presenting cells, have demonstrated effective in controlling the initial of innate immune, while CpG could improve the performance of immune system. To explore the mechanism of CpG enhancing the immune response, we compared different stimulated mouse DCs with systemic approach microarrays. Analysis revealed 1840 differentially expressed genes in H9N2 stimulated group, more than 1728 altered genes in inactive H9N2 group. Investigation also proved that CpG/inactive H9N2 co-stimulation changed 2140 genes, more than that in H9N2 group, strongly demonstrated that CpG improved the performance of inactive H9N2 vaccination. Pathways analysis founded that DCs response rapid to shift in their maturation state, which involved Toll-like receptor (TLR) pathway significantly. Microarrays results were also verified by qRT-PCR with 14 elected representative genes. Further analysis proved that co-stimulatory molecules (CD40, CD80, CD86 and MHC-II), regulat...

Surface and Coatings Technology, 1996

Plasma-assisted chemical vapour deposition (PACVD) has many advantages for the deposition of thin... more Plasma-assisted chemical vapour deposition (PACVD) has many advantages for the deposition of thin films. The use of ions for the energetic bombardment of the film during growth is an important technique for producing dense structures. This paper discusses recent progress in the understanding of the effects of energetic bombardment on the stress levels and structure of films produced by plasma deposition processes. Diagnostic equipment, such as the in situ ellipsometer, energy selecting mass spectrometer and residual gas analyser, enables the PACVD process to be closely monitored so that conditions at the growth surface can be accurately controlled. New plasma sources, such as the helicon plasma source, give increased ion fluxes. The cathodic arc source is also an intense source of highly ionized plasma which can be used for PACVD. The deposition of rugate optical structures based on SiOxNy can be carried out using a PACVD process in which the refractive index profile is created by the computer control of gas flows. The helicon plasma source is useful for the deposition of SiO2 films and has been adapted for use in an ion plating process for the deposition of cubic phase BN. The deposition of amorphous hydrogenated carbon films by PACVD of acetylene in a cathodic arc has been shown to be possible and demonstrates the effect of using a highly ionized plasma for PACVD.

Journal of Medicinal Chemistry, 2013

RhoA and its downstream effector ROCK mediate stress fiber formation and cell contraction through... more RhoA and its downstream effector ROCK mediate stress fiber formation and cell contraction through their effects on the phosphorylation of myosin light chain (MLC). Inhibition of the RhoA/ROCK pathway has proven to be a promising strategy for several indications such as cardiovascular disease, glaucoma, and inflammatory disease. In 2010, our group reported urea-based ROCK inhibitors as potential antiglaucoma agents. These compounds showed potent IC 50 values in enzymatic and cell-based assays and significant intraocular pressure (IOP)-lowering effects in rats (∼7 mmHg). To develop more advanced ROCK inhibitors targeting various potential applications (such as myocardial infarction, erectile dysfunction, multiple sclerosis, etc.) in addition to glaucoma, a thorough SAR for this urea-based scaffold was studied. The detailed optimization process, counter-screening, and in vitro and in vivo DMPK studies are discussed. Potent and selective ROCK inhibitors with various in vivo pharmacokinetic properties were discovered. . A previously disclosed urea-based ROCK inhibitor (1) and its key interactions in the ATP site of In addition to hinge binding to residues E170 and M172, the other three interactions also contributed to the high potency of this urea-based ROCK inhibitor. Article pubs.acs.org/jmc a Reagents and conditions: (a) 1-(Isocyanomethyl)-3-methoxybenzene, DCM; (b) boronic acid pinacol ester, Ph(PPh 3 ) 4 ,K 2 CO 3 , dioxane/H 2 O (4:1), 95°C. Scheme 3. Synthesis of Inhibitors 12a−n a a Reagents and conditions: (a) (i) R 3 -NH 2 , MeOH, r.t; (ii) NaBH 4 ,0°C ; (b) 1-bromo-4-isocyanatobenzene, DCM; (c) boronic acid pinacol ester, Ph(PPh 3 ) 4 ,K 2 CO 3 , dioxane/H 2 O (4:1), 95°C.

Journal of Crystal Growth, 2011

InGaN/GaN quantum wells (QWs) with symmetrical ultra thin (about 0.5nm) low temperature GaN (LT-G... more InGaN/GaN quantum wells (QWs) with symmetrical ultra thin (about 0.5nm) low temperature GaN (LT-GaN) layers bounding each InGaN layer were grown by metal-organic vapor phase epitaxy (MOVPE). From the high resolution X-ray diffraction (HR-XRD) measurement, it showed improved well-barrier interface abruptness compared to the reference MQWs without the LT-GaN layers. In addition, the V-defect density and surface roughness were reduced, especially with the depth of V-defect as low as 0.7nm. Based on the temperature dependence photoluminescence (TDPL) experiments, the internal quantum efficiency (IQE) was increased from 21.2% to 30.1% by inserting the LT-GaN layers. The carrier lifetime obtained from room temperature time resolved photoluminescence (TRPL) measurement was 7.95ns, which was longer than 5.34ns for reference MQWs. These results indicated that these additional symmetrical thin LT-GaN layers enhanced the mobility of indium and gallium atoms as well as suppressed the indium desorption for growth high quality InGaN layers and in turn improved its structural and luminescence properties.

International Journal of Radiation Oncology*Biology*Physics, 2004

Clinical Radiology, 2010

To evaluate image quality and radiation dose for 128-detector prospective electrocardiogram (ECG)... more To evaluate image quality and radiation dose for 128-detector prospective electrocardiogram (ECG)-gated computed tomography coronary angiography (CTCA) compared with a low-dose retrospective ECG-gated imaging protocol. Thirty-one and 47 patients suspected of having coronary artery disease were enrolled into groups examined using prospective and low-dose retrospective ECG-gated CT protocols respectively. All examinations were performed on a 128-detector CT system (Definition AS, Siemens Healthcare, Forchheim, Germany). Prospective CTCA was performed using following parameters: tube voltage 100 kV; tube current 205 mAs; centre of acquisition window 70% of the RR interval. The tube current for low-dose retrospective ECG-gated CTCA was full dose during 40-70% of the RR interval and partial dose for the rest of RR interval. The pitch varied between 0.2 and 0.5 depending on heart rate and patient size. Image quality of coronary arteries was evaluated using a four-point grading scale. The signal-to-noise ratios (SNRs) of enhanced arteries and myocardium were also measured, corresponding contrast-to-noise ratios (CNRs) were calculated, and the radiation doses received were recorded. There was a significant difference in the image quality scores between the retrospective and prospective gating protocols (Chi-square=15.331, p=0.009). There was no significant difference between the SNRs of the contrasted artery and myocardium in these two groups, but the CNRs were increased in the prospective group. The mean radiation dose of prospective gating group was 2.71+/-0.67 mSv (range, 1.67-3.59 mSv), which was significantly lower than that of the retrospective group (p<0.001). Prospective CT angiography can achieve lower radiation dose than that of low-dose retrospective CT angiography, with preserved image quality.

Clinical & Experimental Immunology, 2009

Invasive pneumococcal diseases incur significant mortality, morbidity and economic costs. The mos... more Invasive pneumococcal diseases incur significant mortality, morbidity and economic costs. The most effective strategy currently available to reduce the burden of these diseases is vaccination. In this study, we evaluated the protective efficacy of immunizing mice with caseinolytic protease (ClpP) protein antigen whose gene sequences were shown to be highly conserved in different strains of Streptococcus pneumoniae in an invasive-disease model (intraperitoneal infection model), and protection against invasive challenge with 12 different serotypes of S. pneumoniae was assessed in two murine strains. Our findings demonstrated that active immunization with ClpP and passive immunization with antibodies specific for ClpP could elicit serotypeindependent protection effectively against invasive pneumococcal infection. Therefore, to our knowledge, this study is the first report that immunization with single pneumococcal ClpP protein antigen could protect against such broad-range pneumococal strains, which thus supports the development of ClpP as a human penumococcal vaccine.

Eukaryotic cell, Jan 24, 2015

The global regulatory veA gene governs development and secondary metabolism in numerous fungal sp... more The global regulatory veA gene governs development and secondary metabolism in numerous fungal species, including Aspergillus flavus. This is especially relevant since A. flavus infects crops of agricultural importance worldwide, contaminating them with potent mycotoxins. The most well-known are aflatoxins, cytotoxic and carcinogenic polyketide compounds. The production of aflatoxins, and the expression of genes implicated in the production of these mycotoxins, are veA-dependent. The genes responsible for the synthesis of aflatoxins are clustered, a signature common for genes involved in fungal secondary metabolism. Studies of the A. flavus genome revealed many gene clusters possibly connected to the synthesis of secondary metabolites. Many of these metabolites are still unknown, or the association between a known metabolite with a particular gene cluster has not yet been established. In the present transcriptome study we show that veA is necessary for the expression of a large numb...