Yacouba Nébié - Academia.edu (original) (raw)

Papers by Yacouba Nébié

The Journal of infectious diseases, Jan 18, 2017

The international impact, rapid widespread transmission, and reporting delays during the 2014 Ebo... more The international impact, rapid widespread transmission, and reporting delays during the 2014 Ebola virus disease (EVD) outbreak in West Africa highlighted the need for a global, centralized database to inform outbreak response. The World Health Organization and Emerging and Dangerous Pathogens Laboratory Network addressed this need by supporting the development of a global laboratory database. Specimens were collected in the affected countries from patients and dead bodies meeting the case definitions for EVD. Test results were entered in nationally standardized spreadsheets and consolidated onto a central server. From March 2014 through August 2016, 256,343 specimens tested for EVD were captured in the database. Thirty-one specimen types were collected and a variety of diagnostic tests were performed. Regular analysis of data described functionality of laboratory and response systems, positivity rates, and specimen geographic distribution. With data standardization and end user bu...

African Journal of Laboratory Medicine, 2017

Background: Laboratory-enhanced surveillance is critical for rapidly detecting the potential re-e... more Background: Laboratory-enhanced surveillance is critical for rapidly detecting the potential re-emergence of Ebola virus disease. Rapid diagnostic tests (RDT) for Ebola antigens could expand diagnostic capacity for Ebola virus disease.Objectives: The Guinean National Coordination for Ebola Response conducted a pilot implementation to determine the feasibility of broad screening of patients and corpses with the OraQuick® Ebola RDT.Methods: The implementation team developed protocols and trained healthcare workers to screen patients and corpses in Forécariah prefecture, Guinea, from 15 October to 30 November 2015. Data collected included number of consultations, number of fevers reported or measured, number of tests performed for patients or corpses and results of confirmatory RT-PCR testing. Data on malaria RDT results were collected for comparison. Feedback from Ebola RDT users was collected informally during supervision visits and forums.Results: There were 3738 consultations at th...

Hematology & Transfusion International Journal, 2017

The Journal of Infectious Diseases, 2003

Resume Objectifs : Dans la plupart des pays en developpement, le cytomegalovirus (CMV), le virus... more Resume Objectifs : Dans la plupart des pays en developpement, le cytomegalovirus (CMV), le virus d’Epstein Barr (EBV) et l’Herpes virus 6 (HHV-6) ne sont pas diagnostiques chez les donneurs de sang. Le but de cette etude etait de determiner les prevalences de ces virus, chez les donneurs de sang de la ville de Ouagadougou, Burkina Faso. Materiels et methodes : L’etude a concerne 198 donneurs de sang du centre regional de transfusion sanguine de la ville de Ouagadougou. La PCR multiplexe en temps reel a ete utilisee pour diagnostiquer les trois virus. Les analyses statistiques ont ete faites avec les logiciels Epi info version 6 et SPSS version 17. Les valeurs de P ≤ 0,05 ont ete considerees comme significatives. Resultats : Sur un total de 198 echantillons testes, 18 (9,1%) etaient positifs a au moins un des trois virus, 10 (5,1%) etaient positifs au EBV, 10 (5,1%) positifs au CMV et 12 (6,1%) positifs au HHV-6. Les infections virales etaient plus eleves chez les femmes que chez l...

Haemophilia, 2021

There is a lack of joint recommendations by healthcare professionals (HCP) and patient organizati... more There is a lack of joint recommendations by healthcare professionals (HCP) and patient organizations when a partnership between high and low‐income countries in the field of haemophilia is planned.

Open Journal of Hematology, 2016

Pan African Medical Journal, 2016

Emerging Infectious Diseases, 2016

Blood transfusion = Trasfusione del sangue, Jan 12, 2012

BACKGROUND: Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Fas... more BACKGROUND: Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. MATERIALS AND METHODS: Serum samples were screened for antibodies to HCV using an enzyme-linked immunosorbent assay (ARCHITECT-i1000SR-ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). RESULTS: Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% c...

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Médecine tropicale : revue du Corps de santé colonial, 2011

The aim of this pilot study was to investigate the use of viral genome diagnosis of HIV-1 infecti... more The aim of this pilot study was to investigate the use of viral genome diagnosis of HIV-1 infection in blood donors in the regional blood transfusion center in Ouagadougou, Burkina Faso. This prospective study was carried out from August to December 2009 at the regional blood transfusion center in Ouagadougou (RBTC-O). Detection of HIV-1 was performed by RT-PCR on pooled plasma and individual samples from blood donors. Samples were selected based on reactivity with fourth generation ELISA. ELISA assays on 20 plasma pools demonstrated 10 negative samples, 8 positive and 2 undeterminable. All positive and negative ELISA tests were confirmed by RT-PCR. Findings of RT-PCR on individual samples confirmed those obtained on pooled plasma samples. For the two undeterminable pools, RT-PCR identified one as negative and the other as positive. Individual RT-PCR testing of donations contained in positive and negative pooled plasma samples confirmed negative or positive findings. Because of the ...

Journal of Viral Hepatitis, 2014

High seroprevalence of hepatitis B virus and hepatit is C virus among human immunodeficie ncy vir... more High seroprevalence of hepatitis B virus and hepatit is C virus among human immunodeficie ncy virus carriers in blood donors of Burkina Faso: a need for their screening before HARRT therapy

BACKGROUND: Following World Health Organization recommendations that a quality control (QC) syste... more BACKGROUND: Following World Health Organization recommendations that a quality control (QC) system be implemented in African blood centers, a pilot study of the performance of human immunodeficiency virus anti-body (anti-HIV), hepatitis B surface antigen (HBsAg), and hepatitis C virus antibody (anti-HCV) testing by several Sub-Saharan African blood centers was initiated. STUDY DESIGN AND METHODS: A reference labora-tory sent a panel of 25 samples to six African blood center laboratories. The panel included eight negative samples; four anti-HIV-1–, one anti-HIV-2–, four anti-HCV–, and five HBsAg-positive samples; and three samples consisting of mixtures of two sera to mimic coinfections. Sensitivity, specificity, and overall quality (correct positive or negative status) scores were calculated. RESULTS: From the 21 sets of results obtained (seven for each virus), eight were from rapid tests (two for HIV, three for HBV, and three for HCV) and 13 were from enzyme immunoassays (EIAs; all...