Yang Yu yao - Academia.edu (original) (raw)
Papers by Yang Yu yao
Frontiers in Bioengineering and Biotechnology
The extracellular matrix interacts with cancer cells and is a key factor in the development of ca... more The extracellular matrix interacts with cancer cells and is a key factor in the development of cancer. Traditional two-dimensional models cannot mimic the natural in situ environment of cancer tissues, whereas three-dimensional (3D) models such as spherical culture, bioprinting, and microfluidic approaches can achieve in vitro reproduction of certain structures and components of the tumor microenvironment, including simulation of the hypoxic environment of tumor tissue. However, the lack of a perfusable vascular network is a limitation of most 3D models. Solid tumor growth and metastasis require angiogenesis, and tumor models with microvascular networks have been developed to better understand underlying mechanisms. Tumor-on-a-chip technology combines the advantages of microfluidics and 3D cell culture technology for the simulation of tumor tissue complexity and characteristics. In this review, we summarize progress in constructing tumor-on-a-chip models with efficiently perfused va...
Aging and disease
Ischemic postconditioning (IPostC) is a concept of ischemic stroke treatment, in which several cy... more Ischemic postconditioning (IPostC) is a concept of ischemic stroke treatment, in which several cycles of brief reocclusion after reperfusion are repeated. It is essential to have an accurate understanding of the immune response in IPostC. By using high parametric single-cell mass cytometry, immune cell subsets and characterize their unique functions from ischemic brain and peripheral blood were identified after IPostC. This study enabled us to better understand the immune cell phenotypical and functional characteristics in ischemic brain and peripheral blood at the single-cell and protein levels. Since some cell surface markers can serve as functional markers, reflecting the degree of inflammation, the cell surface marker intensity among different groups was analyzed. The results showed that downregulation of 4E-BP1 and p38 of Microglia and MoDM in the ischemic brain was involved in IPostC-induced protection. In the peripheral blood, downregulation of P38 of CD4 T cell and Treg has also participated in IPostC-induced protection.
Frontiers in Bioengineering and Biotechnology
Mature vasculature is important for the survival of bioengineered tissue constructs, both in vivo... more Mature vasculature is important for the survival of bioengineered tissue constructs, both in vivo and in vitro; however, the fabrication of fully vascularized tissue constructs remains a great challenge in tissue engineering. Indirect three-dimensional (3D) bioprinting refers to a 3D printing technique that can rapidly fabricate scaffolds with controllable internal pores, cavities, and channels through the use of sacrificial molds. It has attracted much attention in recent years owing to its ability to create complex vascular network-like channels through thick tissue constructs while maintaining endothelial cell activity. Biodegradable materials play a crucial role in tissue engineering. Scaffolds made of biodegradable materials act as temporary templates, interact with cells, integrate with native tissues, and affect the results of tissue remodeling. Biodegradable ink selection, especially the choice of scaffold and sacrificial materials in indirect 3D bioprinting, has been the fo...
Frontiers in Bioscience-Landmark
Liquid biopsy has advantages over traditional biopsy, which cannot determine tumor dynamics. As a... more Liquid biopsy has advantages over traditional biopsy, which cannot determine tumor dynamics. As a noninvasive and precise test, liquid biopsy detects biomarkers that carry information on tumor progression and has undergone tremendous development in recent years. Exosome detection is one of the methods of liquid biopsy. Radiotherapy affects the release of exosomes and intercellular communication. Based on the properties, extractability, and detectability of exosomes, key exosomal cargoes after tumor radiotherapy can be used as biomarkers for tumor prognosis. Exosomes after tumor radiotherapy can be used for liquid biopsy. The main applications include (1) predicting radiotherapy efficacy, (2) predicting tumor prognosis, and (3) optimizing the regimen of tumor treatment. This review provides further research directions for liquid biopsy after tumor radiotherapy.
Water
Rapid urbanization influences the landscape pattern of impervious surfaces, and potentially affec... more Rapid urbanization influences the landscape pattern of impervious surfaces, and potentially affects surface water quality. Using ArcGIS and Fragstats, this study analyzed the temporal change of the landscape pattern of impervious surfaces in Shanghai over the past 45 years, and its driving forces and impact on water quality were also analyzed. The results show that both low and high impervious surfaces showed different degrees of expansion, and as a result, the pervious surfaces and water area reduced by 40.1% and 13.8%, respectively. It proves that the fragmentation and diversity of impervious surfaces in Shanghai notably increased in the past decades, and especially the low and high impervious surfaces show substantial changes. The primary driving forces of the landscape pattern change are population density, unit area Gross Domestic Product (GDP), and the percentage of primary industry. The result of Redundancy analysis (RDA) is that the explanatory ability of landscape pattern t...
Frontiers in Oncology, 2021
BackgroundMYCN is an oncogenic transcription factor of the MYC family and plays an important role... more BackgroundMYCN is an oncogenic transcription factor of the MYC family and plays an important role in the formation of tissues and organs during development before birth. Due to the difficulty in drugging MYCN directly, revealing the molecules in MYCN regulatory networks will help to identify effective therapeutic targets.MethodsWe utilized network controllability theory, a recent developed powerful tool, to identify the potential drug target around MYCN based on Protein-Protein interaction network of MYCN. First, we constructed a Protein-Protein interaction network of MYCN based on public databases. Second, network control analysis was applied on network to identify driver genes and indispensable genes of the MYCN regulatory network. Finally, we developed a novel integrated approach to identify potential drug targets for regulating the function of the MYCN regulatory network.ResultsWe constructed an MYCN regulatory network that has 79 genes and 129 interactions. Based on network con...
Water, 2022
Large quantity of rivers have disappeared during rapid urbanization in China’s Yangtze River Delt... more Large quantity of rivers have disappeared during rapid urbanization in China’s Yangtze River Delta, and it is difficult to define the changes in the river network owing to its high dense and complex reticular structure. Shanghai’s Pudong New Area (SPNA) is a typical area of rapid urbanization in the delta. A Comprehensive River-network Indicator System (CRIS), including quantity and area, geomorphologic structure, and landscape connectivity indicators, was established to characterize the changes in the dense reticular river network in SPNA from 1965 to 2010. The percentage of urban area rapidly increased from 22.52% to 59.49% in SPNA, whereas that of water surface (Wp) decreased from 10.57% to 7.23% during the same period. The changes in river network displayed a clear spatial gradient, and the closer the functional area is to the city center, the earlier and faster the changes in the rivers. CRIS obviously changed when the percentage of built-up area increased from 10% to 30%; howe...
Frontiers in Genetics, 2021
BackgroundBladder cancer is a common malignant tumor characterized by high mortality and high man... more BackgroundBladder cancer is a common malignant tumor characterized by high mortality and high management costs; however, it lacks useful molecular prognostic markers. Tribbles pseudokinase 3 (TRIB3) is a pseudokinase that participates in cell tumor progression and metabolism and whose function in bladder cancer is not precisely known.Main MethodsWe downloaded transcriptome data and clinical data of bladder cancer from associated databases and extracted the expression matrix of TRIB3 for multiple bioinformatics analysis. RT-PCR detected the expression of TRIB3 in bladder cancer cells. After knockdown of TRIB3 with siRNA, we investigated TRIB3 function using CCK8, Cell Cycle and Transwell assays.Key FindingsKaplan–Meier analysis of TRIB3 in the four cohorts showed that high expression of TRIB3 correlated with poor outcome. Expression of TRIB3 positively correlated with stage and grade and down-regulation of TRIB3 expression significantly inhibited proliferation, migration and cell cyc...
SSRN Electronic Journal, 2021
We examine the transition to and persistence of working from home (WFH) by firms after the Covid-... more We examine the transition to and persistence of working from home (WFH) by firms after the Covid-19 shock. Using job posting data from a leading online job portal in China and exploiting the Covid-19 pandemic as a quasi-experiment inducing the short-run WFH take-up of firms, we find a substantial and persistent increase in the share of WFH jobs post Covid-19. The WFH share increase in job posting is larger in firms with lower pre-Covid WFH adoption, consistent with the learning effect from temporary shutdown policies. Firms with greater potential for remote work, measured by the teleworkability index à la Dingel & Neiman (2020), also experience larger increase in WFH job postings. Given that WFH jobs provide higher salaries and have higher educational requirements, our findings suggest that WFH is here to stay and thus have longterm implications on firm productivity and labor market inequality.
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](International journal of molecular sciences, 2018
The development of the female gametophyte (FG) is one of the key processes of life cycle alterati... more The development of the female gametophyte (FG) is one of the key processes of life cycle alteration between the haploid gametophyte and the diploid sporophytes in plants and it is required for successful seed development after fertilization. It is well demonstrated that free nuclear mitosis (FNM) of FG is crucial for the development of the ovule. However, studies of the molecular mechanism of ovule and FG development focused mainly on angiosperms, such as and further investigation of gymnosperms remains to be completed. Here, Illumina sequencing of six transcriptomic libraries obtained from developing and abortive ovules at different stages during free nuclear mitosis of magagametophyte (FNMM) was used to acquire transcriptome data and gene expression profiles of . Six cDNA libraries generated a total of 71.0 million high-quality clean reads that aligned with 63,449 unigenes and the comparison between developing and abortive ovules identified 7174 differentially expressed genes (DEG...
European radiology, Jan 14, 2016
To retrospectively assess the safety and efficacy of percutaneous vertebroplasty (PVP) for painfu... more To retrospectively assess the safety and efficacy of percutaneous vertebroplasty (PVP) for painful osteolytic spinal metastases when treating more than three vertebrae per session. A total of 153 patients with painful osteolytic spinal metastases underwent PVP. Group A patients (n = 93) underwent PVP at up to three vertebral levels per session. Group B patients (n = 60) underwent PVP at more than three levels in one session. Pain, quality of life (QoL), and mobility were assessed before and after PVP. Minor and major complications were systematically assessed. Both groups experienced significant pain relief and QoL improvement after the intervention (p < 0.001). Mobility improvement was observed in both groups, despite worse mobility status before PVP in group B compared with group A. There was no significant difference between the two groups throughout the follow-up period in overall pain relief and improvement in QoL and mobility. There was also no significant difference betwee...
Experimental and Therapeutic Medicine, 2016
The present study aimed to evaluate the efficacy and safety of acetyl-L-carnitine (ALC) for the t... more The present study aimed to evaluate the efficacy and safety of acetyl-L-carnitine (ALC) for the treatment of chemotherapy-induced peripheral neuropathy (CIPN). The study was carried out as a prospective, randomized, double-blind, placebo-controlled and paralleled clinical study. A total of 239 patients with CIPN were selected as the study subjects. Of the 239 subjects, 118 subjects received 3 g/day ALC orally for 8 weeks and 121 received a placebo. The primary endpoint was improvement of peripheral neuropathy by at least one grade. Patient status was assessed at week 4, 8 and 12 after enrollment into the study. In both the full analysis set (FAS) and the per-protocol set (PPS), peripheral sensory neuropathy was significantly ameliorated in the ALC group with 50.5 and 51.6% patients meeting the primary endpoint at week 8, compared with 24.1 and 23.1% of patients in the placebo group (P<0.001 in both sets). Secondary endpoints, such as the nerve electrophysiological examination and the Karnofsky physical score were also significantly improved in patients receiving ALC treatment, as compared with the placebo group (FAS, P= 0.0463 and P= 0.022; PPS, P= 0.0076 and P= 0.0064, respectively). Cancer-associated fatigue was significantly alleviated following ALC treatment in the PPS (P= 0.0135). In the safety analysis set, the difference in adverse events incidence between the two groups was not statistically significant (P= 0.3903). There were only two severe adverse events in the ALC group, which were not associated with the effect of ALC. In conclusion, the results of the present study demonstrated that in Chinese patients with cancer, oral administration of ALC is effective at ameliorating peripheral sensory neuropathy induced by chemotherapy, as well as reducing of cancer-associated fatigue and improving physical conditions.
Molecular medicine reports, 2014
Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the... more Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT). EMT is a key process in tumor metastasis. Although overexpression of KLF8 has been observed in a variety of human tumor types, the role of KLF8 in human osteosarcoma is yet to be elucidated. The present study aimed to investigate the biological impact of KLF8 on Saos-2 osteosarcoma cells. KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method. Cell proliferation and cell cycle distribution were evaluated using 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and colony formation assays, and flow cytometry, respectively. Cell invasion was analyzed using a Transwell® invasion assay. Knockdown of KLF8 was found to significantly inhibit proliferation and invasion in osteosarcoma cells. These data suggest that KLF8 m...
Frontiers in Bioengineering and Biotechnology
The extracellular matrix interacts with cancer cells and is a key factor in the development of ca... more The extracellular matrix interacts with cancer cells and is a key factor in the development of cancer. Traditional two-dimensional models cannot mimic the natural in situ environment of cancer tissues, whereas three-dimensional (3D) models such as spherical culture, bioprinting, and microfluidic approaches can achieve in vitro reproduction of certain structures and components of the tumor microenvironment, including simulation of the hypoxic environment of tumor tissue. However, the lack of a perfusable vascular network is a limitation of most 3D models. Solid tumor growth and metastasis require angiogenesis, and tumor models with microvascular networks have been developed to better understand underlying mechanisms. Tumor-on-a-chip technology combines the advantages of microfluidics and 3D cell culture technology for the simulation of tumor tissue complexity and characteristics. In this review, we summarize progress in constructing tumor-on-a-chip models with efficiently perfused va...
Aging and disease
Ischemic postconditioning (IPostC) is a concept of ischemic stroke treatment, in which several cy... more Ischemic postconditioning (IPostC) is a concept of ischemic stroke treatment, in which several cycles of brief reocclusion after reperfusion are repeated. It is essential to have an accurate understanding of the immune response in IPostC. By using high parametric single-cell mass cytometry, immune cell subsets and characterize their unique functions from ischemic brain and peripheral blood were identified after IPostC. This study enabled us to better understand the immune cell phenotypical and functional characteristics in ischemic brain and peripheral blood at the single-cell and protein levels. Since some cell surface markers can serve as functional markers, reflecting the degree of inflammation, the cell surface marker intensity among different groups was analyzed. The results showed that downregulation of 4E-BP1 and p38 of Microglia and MoDM in the ischemic brain was involved in IPostC-induced protection. In the peripheral blood, downregulation of P38 of CD4 T cell and Treg has also participated in IPostC-induced protection.
Frontiers in Bioengineering and Biotechnology
Mature vasculature is important for the survival of bioengineered tissue constructs, both in vivo... more Mature vasculature is important for the survival of bioengineered tissue constructs, both in vivo and in vitro; however, the fabrication of fully vascularized tissue constructs remains a great challenge in tissue engineering. Indirect three-dimensional (3D) bioprinting refers to a 3D printing technique that can rapidly fabricate scaffolds with controllable internal pores, cavities, and channels through the use of sacrificial molds. It has attracted much attention in recent years owing to its ability to create complex vascular network-like channels through thick tissue constructs while maintaining endothelial cell activity. Biodegradable materials play a crucial role in tissue engineering. Scaffolds made of biodegradable materials act as temporary templates, interact with cells, integrate with native tissues, and affect the results of tissue remodeling. Biodegradable ink selection, especially the choice of scaffold and sacrificial materials in indirect 3D bioprinting, has been the fo...
Frontiers in Bioscience-Landmark
Liquid biopsy has advantages over traditional biopsy, which cannot determine tumor dynamics. As a... more Liquid biopsy has advantages over traditional biopsy, which cannot determine tumor dynamics. As a noninvasive and precise test, liquid biopsy detects biomarkers that carry information on tumor progression and has undergone tremendous development in recent years. Exosome detection is one of the methods of liquid biopsy. Radiotherapy affects the release of exosomes and intercellular communication. Based on the properties, extractability, and detectability of exosomes, key exosomal cargoes after tumor radiotherapy can be used as biomarkers for tumor prognosis. Exosomes after tumor radiotherapy can be used for liquid biopsy. The main applications include (1) predicting radiotherapy efficacy, (2) predicting tumor prognosis, and (3) optimizing the regimen of tumor treatment. This review provides further research directions for liquid biopsy after tumor radiotherapy.
Water
Rapid urbanization influences the landscape pattern of impervious surfaces, and potentially affec... more Rapid urbanization influences the landscape pattern of impervious surfaces, and potentially affects surface water quality. Using ArcGIS and Fragstats, this study analyzed the temporal change of the landscape pattern of impervious surfaces in Shanghai over the past 45 years, and its driving forces and impact on water quality were also analyzed. The results show that both low and high impervious surfaces showed different degrees of expansion, and as a result, the pervious surfaces and water area reduced by 40.1% and 13.8%, respectively. It proves that the fragmentation and diversity of impervious surfaces in Shanghai notably increased in the past decades, and especially the low and high impervious surfaces show substantial changes. The primary driving forces of the landscape pattern change are population density, unit area Gross Domestic Product (GDP), and the percentage of primary industry. The result of Redundancy analysis (RDA) is that the explanatory ability of landscape pattern t...
Frontiers in Oncology, 2021
BackgroundMYCN is an oncogenic transcription factor of the MYC family and plays an important role... more BackgroundMYCN is an oncogenic transcription factor of the MYC family and plays an important role in the formation of tissues and organs during development before birth. Due to the difficulty in drugging MYCN directly, revealing the molecules in MYCN regulatory networks will help to identify effective therapeutic targets.MethodsWe utilized network controllability theory, a recent developed powerful tool, to identify the potential drug target around MYCN based on Protein-Protein interaction network of MYCN. First, we constructed a Protein-Protein interaction network of MYCN based on public databases. Second, network control analysis was applied on network to identify driver genes and indispensable genes of the MYCN regulatory network. Finally, we developed a novel integrated approach to identify potential drug targets for regulating the function of the MYCN regulatory network.ResultsWe constructed an MYCN regulatory network that has 79 genes and 129 interactions. Based on network con...
Water, 2022
Large quantity of rivers have disappeared during rapid urbanization in China’s Yangtze River Delt... more Large quantity of rivers have disappeared during rapid urbanization in China’s Yangtze River Delta, and it is difficult to define the changes in the river network owing to its high dense and complex reticular structure. Shanghai’s Pudong New Area (SPNA) is a typical area of rapid urbanization in the delta. A Comprehensive River-network Indicator System (CRIS), including quantity and area, geomorphologic structure, and landscape connectivity indicators, was established to characterize the changes in the dense reticular river network in SPNA from 1965 to 2010. The percentage of urban area rapidly increased from 22.52% to 59.49% in SPNA, whereas that of water surface (Wp) decreased from 10.57% to 7.23% during the same period. The changes in river network displayed a clear spatial gradient, and the closer the functional area is to the city center, the earlier and faster the changes in the rivers. CRIS obviously changed when the percentage of built-up area increased from 10% to 30%; howe...
Frontiers in Genetics, 2021
BackgroundBladder cancer is a common malignant tumor characterized by high mortality and high man... more BackgroundBladder cancer is a common malignant tumor characterized by high mortality and high management costs; however, it lacks useful molecular prognostic markers. Tribbles pseudokinase 3 (TRIB3) is a pseudokinase that participates in cell tumor progression and metabolism and whose function in bladder cancer is not precisely known.Main MethodsWe downloaded transcriptome data and clinical data of bladder cancer from associated databases and extracted the expression matrix of TRIB3 for multiple bioinformatics analysis. RT-PCR detected the expression of TRIB3 in bladder cancer cells. After knockdown of TRIB3 with siRNA, we investigated TRIB3 function using CCK8, Cell Cycle and Transwell assays.Key FindingsKaplan–Meier analysis of TRIB3 in the four cohorts showed that high expression of TRIB3 correlated with poor outcome. Expression of TRIB3 positively correlated with stage and grade and down-regulation of TRIB3 expression significantly inhibited proliferation, migration and cell cyc...
SSRN Electronic Journal, 2021
We examine the transition to and persistence of working from home (WFH) by firms after the Covid-... more We examine the transition to and persistence of working from home (WFH) by firms after the Covid-19 shock. Using job posting data from a leading online job portal in China and exploiting the Covid-19 pandemic as a quasi-experiment inducing the short-run WFH take-up of firms, we find a substantial and persistent increase in the share of WFH jobs post Covid-19. The WFH share increase in job posting is larger in firms with lower pre-Covid WFH adoption, consistent with the learning effect from temporary shutdown policies. Firms with greater potential for remote work, measured by the teleworkability index à la Dingel & Neiman (2020), also experience larger increase in WFH job postings. Given that WFH jobs provide higher salaries and have higher educational requirements, our findings suggest that WFH is here to stay and thus have longterm implications on firm productivity and labor market inequality.
International journal of molecular sciences, 2018
The development of the female gametophyte (FG) is one of the key processes of life cycle alterati... more The development of the female gametophyte (FG) is one of the key processes of life cycle alteration between the haploid gametophyte and the diploid sporophytes in plants and it is required for successful seed development after fertilization. It is well demonstrated that free nuclear mitosis (FNM) of FG is crucial for the development of the ovule. However, studies of the molecular mechanism of ovule and FG development focused mainly on angiosperms, such as and further investigation of gymnosperms remains to be completed. Here, Illumina sequencing of six transcriptomic libraries obtained from developing and abortive ovules at different stages during free nuclear mitosis of magagametophyte (FNMM) was used to acquire transcriptome data and gene expression profiles of . Six cDNA libraries generated a total of 71.0 million high-quality clean reads that aligned with 63,449 unigenes and the comparison between developing and abortive ovules identified 7174 differentially expressed genes (DEG...
European radiology, Jan 14, 2016
To retrospectively assess the safety and efficacy of percutaneous vertebroplasty (PVP) for painfu... more To retrospectively assess the safety and efficacy of percutaneous vertebroplasty (PVP) for painful osteolytic spinal metastases when treating more than three vertebrae per session. A total of 153 patients with painful osteolytic spinal metastases underwent PVP. Group A patients (n = 93) underwent PVP at up to three vertebral levels per session. Group B patients (n = 60) underwent PVP at more than three levels in one session. Pain, quality of life (QoL), and mobility were assessed before and after PVP. Minor and major complications were systematically assessed. Both groups experienced significant pain relief and QoL improvement after the intervention (p < 0.001). Mobility improvement was observed in both groups, despite worse mobility status before PVP in group B compared with group A. There was no significant difference between the two groups throughout the follow-up period in overall pain relief and improvement in QoL and mobility. There was also no significant difference betwee...
Experimental and Therapeutic Medicine, 2016
The present study aimed to evaluate the efficacy and safety of acetyl-L-carnitine (ALC) for the t... more The present study aimed to evaluate the efficacy and safety of acetyl-L-carnitine (ALC) for the treatment of chemotherapy-induced peripheral neuropathy (CIPN). The study was carried out as a prospective, randomized, double-blind, placebo-controlled and paralleled clinical study. A total of 239 patients with CIPN were selected as the study subjects. Of the 239 subjects, 118 subjects received 3 g/day ALC orally for 8 weeks and 121 received a placebo. The primary endpoint was improvement of peripheral neuropathy by at least one grade. Patient status was assessed at week 4, 8 and 12 after enrollment into the study. In both the full analysis set (FAS) and the per-protocol set (PPS), peripheral sensory neuropathy was significantly ameliorated in the ALC group with 50.5 and 51.6% patients meeting the primary endpoint at week 8, compared with 24.1 and 23.1% of patients in the placebo group (P<0.001 in both sets). Secondary endpoints, such as the nerve electrophysiological examination and the Karnofsky physical score were also significantly improved in patients receiving ALC treatment, as compared with the placebo group (FAS, P= 0.0463 and P= 0.022; PPS, P= 0.0076 and P= 0.0064, respectively). Cancer-associated fatigue was significantly alleviated following ALC treatment in the PPS (P= 0.0135). In the safety analysis set, the difference in adverse events incidence between the two groups was not statistically significant (P= 0.3903). There were only two severe adverse events in the ALC group, which were not associated with the effect of ALC. In conclusion, the results of the present study demonstrated that in Chinese patients with cancer, oral administration of ALC is effective at ameliorating peripheral sensory neuropathy induced by chemotherapy, as well as reducing of cancer-associated fatigue and improving physical conditions.
Molecular medicine reports, 2014
Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the... more Krüppel-like factor 8 (KLF8) is a transcription factor that is important in the regulation of the cell cycle and has a critical role in oncogenic transformation and epithelial to mesenchymal transition (EMT). EMT is a key process in tumor metastasis. Although overexpression of KLF8 has been observed in a variety of human tumor types, the role of KLF8 in human osteosarcoma is yet to be elucidated. The present study aimed to investigate the biological impact of KLF8 on Saos-2 osteosarcoma cells. KLF8 gene expression was knocked down in vitro using a lentivirus-mediated small interfering (si)RNA method. Cell proliferation and cell cycle distribution were evaluated using 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide and colony formation assays, and flow cytometry, respectively. Cell invasion was analyzed using a Transwell® invasion assay. Knockdown of KLF8 was found to significantly inhibit proliferation and invasion in osteosarcoma cells. These data suggest that KLF8 m...