Yassin NA - Academia.edu (original) (raw)

Papers by Yassin NA

Research paper thumbnail of Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic

Pharmacia

Plasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs... more Plasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs). This study aims to detect five PMQR genes among FQs-resistant Klebsiella pneumoniae isolated from various clinical specimens. Out of 120 K. pneumoniae isolates, 68 FQs-resistance K. pneumoniae were included in a molecular study. Standard microbiological tests were used for identification and antimicrobial susceptibility. For the detection of PMQR genes, conventional polymerase chain reaction was used. A molecular study revealed that (73.5%) of samples harbored PMQR genes, and among them, 58% were co-carriages of PMQR gene variants. Aac (6’)-Ib-cr gene was predominant (47.1%) among samples, and qepA had the lowest percentage (11.8%), qnr genes were (32.4%) (29.4%) (20.6%) qnrS, qnrB, and qnrA respectively. Overall, high percentages of PMQR genes were detected, and almost all of samples were phenotypically resistant to ciprofloxacin. As well, there was a significant statistical relation...

Research paper thumbnail of Bacteriological and Molecular Characterization of Salmonella Species Isolated from Humans and Chickens in Sharkia Governorate, Egypt

Zagazig Veterinary Journal, 2017

This study was carried out to investigate the occurrence of Salmonella species in 690 different s... more This study was carried out to investigate the occurrence of Salmonella species in 690 different samples collected from Sharkia Governorate, Egypt. The samples comprised of chicken meat, organs, eggs, cloacal swabs and wooden surface swabs from pluck shop outlets and hand swabs from workers. In addition, stool swabs from patients with gastroenteritis attending the Outpatient Clinics at Zagazig and Abo-Hammad cities. Salmonella spp. were detected in 5.9%, 2.6%, 4.2%, 10.4%, 10% and 10% of chicken meat, organs, egg shell, cloacal swabs, surface swabs and hand swabs, respectively. The isolation rate from stool swabs was 0.8%. Biochemical Identification revealed that Salmonella spp. were identified in 129 out of 690 examined samples (18.7%). Meanwhile, molecular identification using invA gene revealed that only 29 Salmonella isolates were detected (4.2%). S. Typhimurium and S. Enteritidis were identified from different samples with the percentages of 1.2 and 1.01, respectively. The other identified Salmonella serovars were S. Newport (0.9%), S. Kentucky (0.7%), and S. Infantis (0.4%). Virulence associated genes including avrA, hilA and pef were identified in 100, 91.3 and 10.3% of the examined isolates. In conclusion, a proportion of chicken carcasses and giblets sold in Sharkia Governorate, Egypt was contaminated with Salmonella spp. including potentially virulent S. Typhimurium and S. Enteritidis, posing risk for human consumers.

Research paper thumbnail of Prevalence of metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from diabetic foot ulcer patients

Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 2011

Metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa strains have been reported to be an... more Metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa strains have been reported to be an important cause of nosocomial infections. There is not enough information from India regarding their prevalence in diabetic foot ulcer (DFU) patients. The present study was undertaken over a period of two year from December 2008 to March 2011 to study the incidence of MBL producing P. aeruginosa isolated from 162 DFU patients with various grades of ulcer (Texas classification). Forty isolates of P. aeruginosa were obtained from patients. These isolates were subjected to susceptibility testing to anti-pseudomonal drugs as per Clinical Laboratory and Standards Institute (CLSI) guidelines, and were further screened for the production of MBL by disc potentiation testing using ethylenediaminetetraacetic acid (EDTA)-impregnated imipenem and meropenem discs. Of the 40 isolates of P. aeruginosa, 22 (55%) isolates were found resistant to carbapenems (imipenem) and 18 (81.1%) were found to be MBL producers using imipenem+(EDTA) and 15(68.1%) by meropenem+EDTA. This rapid dissemination of MBL producers is worrisome and necessitates the implementation of not just surveillance studies but also proper and judicious selection of antibiotics, especially carbapenems.

Research paper thumbnail of Bacteriological and Molecular Characterization of Diarrheagenic Escherichia Coli Pathotypes From Children in Duhok City, Iraq

Science Journal of University of Zakho, 2020

Background: A variety of diarrheagenic E coli (DEC) are responsible for causing different types o... more Background: A variety of diarrheagenic E coli (DEC) are responsible for causing different types of diarrhea in children especially in developing courtiers. Objectives: This study was primarily aimed to isolate and bacteriological characterizing of E coli from diarrheic infant stool and to investigate their antibiotic resistance patterns and then using molecular identification of DEC pathotypes for better discrimination. Methods: Total of 400 fresh stools specimens were collected from children with diarrhea in Heevi Hospital in Duhok city, Iraq. The samples were cultured on selective media such as (MacConkey and MacConkey sorbitol agar). Colonies were identified through biochemical reaction and VITEK 2 system and then antibiotic susceptibility profiles were determined. Results: A total of 349(87.2%) samples were yielded positive for growth of E coli. Out of these, 50 phenotypically-identified E coli were then subjected to PCR assay targeting certain virulence factors (alt, eae, sxt1 ...

Research paper thumbnail of Les fortifications de Jérusalem à l'époque de la Royauté de David et de Salomon

Research paper thumbnail of Carrier state of methicillin-resistant/sensitive Staphylococcus aureus in nasal and skin among physical education students, Duhok University, Iraq

Diagnostic and Therapeutic Study, 2013

In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially thos... more In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially those involved in athletic activities. (CA-MRSA) infections have become an increasing problem in athletics as well as in the general population during recent years. This study purposed to research MRSA/MSSA Staphylococcus aureus carriage in both the nose and skin and evaluation of their antibiotic susceptibility tests among students in physical education, Duhok University, Iraq. For this purpose, a total of 266 swabs that distributed between 133 nasal swabs and 133 skin swabs were taken from students between Marches to May 2013. The swab samples were examined through microbiological methods. All the isolates were tested for antimicrobial susceptibility by the disk diffusion technique according to the Clinical and Laboratory Standards Institute guidelines on Muller Hinton Agar. The prevalence of Staphylococcus aureus was 52(19.5%) over total number (266|) of nasal and skin swabs examined, of them 36(69%) and 16(31%) were methicillin-resistant S aureus MRSA and methicillin-sensitive S aureus MSSA, respectively. where, high prevalence of MRSA were recorded among nasal samples. MRSA expressed high percentage of resistance collectively to all antibiotics used. Generally, methicillin-sensitive S aureus MSSA exhibited low percentage of resistance collectively to all antibiotics used. This study highlighted that students submitted of Physical Education College might have been contaminated with MSSA/MRSA in their nose and skin during their study phase. That MSSA/MRSA being an important pathogen is seen in students taking a physical education.

Research paper thumbnail of Incidence and Resistotyping Profiles of Bacillus subtilis Isolated from Azadi Teaching Hospital in Duhok City, Iraq

Materia Socio Medica, 2012

Bacillus subtilis are opportunistic, spore forming bacteria, common soil inhabitants, and may fre... more Bacillus subtilis are opportunistic, spore forming bacteria, common soil inhabitants, and may frequently contaminate foods and widely distributed in hospital environments. This paper purposes to find out the incidence of Bacillus subtilis from various sources and locations at Azadi Teaching Hospital in Duhok city, Iraq. The susceptibility test and resistotyping (antibiotypes) profile of isolates were also studied. Samples, using sterile cotton swabs, were collected from various sources and locations and plated on Blood agar, chocolate agar and MacConkey agar. The recovered isolates were identified by routine procedures. Antibiotics susceptibility and resistant profiles to eight selected antibiotics were performed by the disc diffusion method using Mueller-Hinton agar. Out of the 128 samples collected 84 samples were yielded bacterial growth, of them 31(24.2%) were Bacillus subtilis. Moreover; other bacterial groups were isolated and identified. The susceptibility test of Bacillus subtilis isolates; the organism exhibited high susceptibility to gentamicin (96.7%) and ciprofloxacin (93.5%), while cephalexin (25.8%), cefotaxime (19.3%) and ampicillin (16.2%) demonstrated the lowest susceptibility rate. Resistotyping (antibiotypes) profiles for the isolates of Bacillus subtilis were determined. Out of 31 isolates, 22 of them were multiple resistant and belonged to 3 resistotype patterns; resistotype 1 was predominant among isolates. This study shows that there is an increased rate of incidence of Bacillus subtilis in hospital environments and some of these isolates were multi-drug resistant and showed different resistotyping profiles.

Research paper thumbnail of Laboratory evaluation of urine culture and drug resistance in outpatients clinically suspected of urinary tract infections Dr. Najim A. Yassin, Ph.D *

Rawal Medical Journal, 2012

This paper purpose to identify common bacterial etiologic agents causing urinary tract infection ... more This paper purpose to identify common bacterial etiologic agents causing urinary tract infection among outpatients with gender and age distribution visiting Azadi teaching hospital in Duhok city, Iraq. Of the 223 urine specimens cultured, 126 (56.5%) had a positive urine culture, of them 111(88%) female and 15(12%) male. Infections rate were higher among those aged 20-30 years in both gender. Escherichia coli was found to be the most frequent causative agent of UTIs (51.6%) followed by Enterococcus faecalis (27.8.3%), Klebsiella pneumonia (9.5%), Enterobacter aeruginosa (4.8%), Staphylococcus aureus (3.9%) and Pseudomonas aeruginosa (2.4%) of the cases. Escherichia coli showed variable susceptibility rate to different antibiotics as 90.5%, 84.7%, 71%, 46.2%, 43.1%, 43.1%, 41.6%, 35.4%, 33.9% and 21.6% of the isolates were found to be susceptibility to amikacin, nitrofuratoin, rifampicin, nalidixic acid, trimethoprim, gentamicin, ciprofloxacin, cephalothin, cefotaxime and amoxicillin, respectively. A total of 5 different resistotype patterns were obtained with Escherichia coli isolates; common resistotype was 1. This study shows increased rate of urinary tract infections in female than male population in age groups of 20+ and Escherichia coli were the most frequent causative agent and showed multi-drug resistance with different resistotyping patterns.

Research paper thumbnail of Study of Predictive Value of Pediatric Risk of Mortality (PRISM) Score in Children with End Stage Liver Disease and Fulminant Hepatic Failure

Indian Journal of Pediatrics - INDIAN J PEDIATR, 2011

Objective To evaluate the use of the PRISM score as a predictor of outcome in patients with end ... more Objective To evaluate the use of the PRISM score as a predictor of outcome in patients with end stage liver disease (ESLD) and fulminant hepatic failure (FHF). Methods The present study included 30 patients with ESLD and FHF, with ages ranging from 2 to 108 months, who were admitted to the Emergency room (ER) and the Pediatric Hepatology Unit at Cairo University Children’s Hospital (tertiary referral hospital) over a six-month-period from May through October 2008. Survivors were followed up for 6 months. Two other scores were also calculated, the PELD score and the Child-Pugh score. The outcome was defined as survivors and deceased. Results Deceased patients as compared with survivors were significantly younger (median age 7 vs. 24 months, p = 0.003). A ROC curve was constructed for the PRISM score, the predicted death rate (PDR) and the PELD score in the 30 patients. PRISM score was significantly associated with mortality (p = 0.04). The best cut off value was 9.5 (70.6% sensiti...

Research paper thumbnail of Honey as therapeutic agent for infectious diseases

Diagnostic and Therapeutic Study, 2013

Research paper thumbnail of CARRIER STATE OF METHICILLIN – RESISTANT/SENSITIVE STAPHYLOCOCCUS AUREUS IN NSAL AND SKIN AMONG PHYSICAL EDUCATION STUDENTS, DUHOK UNIVERSITY, IRAQ

In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially thos... more In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially those involved in athletic activities. (CA-MRSA) infections have become an increasing problem in athletics as well as in the general population during recent years. This study purposed to research MRSA/MSSA Staphylococcus aureus carriage in both the nose and skin and evaluation of their antibiotic susceptibility tests among students in physical education, Duhok University, Iraq. For this purpose, a total of 266 swabs that distributed between 133 nasal swabs and 133 skin swabs were taken from students between Marches to May 2013. The swab samples were examined through microbiological methods. All the isolates were tested for antimicrobial susceptibility by the disk diffusion technique according to the Clinical and Laboratory Standards Institute guidelines on Muller Hinton Agar. The prevalence of Staphylococcus aureus was 52(19.5%) over total number (266|) of nasal and skin swabs examined, of them 36(69%) and 16(31%) were methicillin-resistant S aureus MRSA and methicillin-sensitive S aureus MSSA, respectively. where, high prevalence of MRSA were recorded among nasal samples. MRSA expressed high percentage of resistance collectively to all antibiotics used. Generally, methicillin-sensitive S aureus MSSA exhibited low percentage of resistance collectively to all antibiotics used. This study highlighted that students submitted of Physical Education College might have been contaminated with MSSA/MRSA in their nose and skin during their study phase. That MSSA/MRSA being an important pathogen is seen in students taking a physical education.

Research paper thumbnail of Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic

Pharmacia

Plasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs... more Plasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs). This study aims to detect five PMQR genes among FQs-resistant Klebsiella pneumoniae isolated from various clinical specimens. Out of 120 K. pneumoniae isolates, 68 FQs-resistance K. pneumoniae were included in a molecular study. Standard microbiological tests were used for identification and antimicrobial susceptibility. For the detection of PMQR genes, conventional polymerase chain reaction was used. A molecular study revealed that (73.5%) of samples harbored PMQR genes, and among them, 58% were co-carriages of PMQR gene variants. Aac (6’)-Ib-cr gene was predominant (47.1%) among samples, and qepA had the lowest percentage (11.8%), qnr genes were (32.4%) (29.4%) (20.6%) qnrS, qnrB, and qnrA respectively. Overall, high percentages of PMQR genes were detected, and almost all of samples were phenotypically resistant to ciprofloxacin. As well, there was a significant statistical relation...

Research paper thumbnail of Bacteriological and Molecular Characterization of Salmonella Species Isolated from Humans and Chickens in Sharkia Governorate, Egypt

Zagazig Veterinary Journal, 2017

This study was carried out to investigate the occurrence of Salmonella species in 690 different s... more This study was carried out to investigate the occurrence of Salmonella species in 690 different samples collected from Sharkia Governorate, Egypt. The samples comprised of chicken meat, organs, eggs, cloacal swabs and wooden surface swabs from pluck shop outlets and hand swabs from workers. In addition, stool swabs from patients with gastroenteritis attending the Outpatient Clinics at Zagazig and Abo-Hammad cities. Salmonella spp. were detected in 5.9%, 2.6%, 4.2%, 10.4%, 10% and 10% of chicken meat, organs, egg shell, cloacal swabs, surface swabs and hand swabs, respectively. The isolation rate from stool swabs was 0.8%. Biochemical Identification revealed that Salmonella spp. were identified in 129 out of 690 examined samples (18.7%). Meanwhile, molecular identification using invA gene revealed that only 29 Salmonella isolates were detected (4.2%). S. Typhimurium and S. Enteritidis were identified from different samples with the percentages of 1.2 and 1.01, respectively. The other identified Salmonella serovars were S. Newport (0.9%), S. Kentucky (0.7%), and S. Infantis (0.4%). Virulence associated genes including avrA, hilA and pef were identified in 100, 91.3 and 10.3% of the examined isolates. In conclusion, a proportion of chicken carcasses and giblets sold in Sharkia Governorate, Egypt was contaminated with Salmonella spp. including potentially virulent S. Typhimurium and S. Enteritidis, posing risk for human consumers.

Research paper thumbnail of Prevalence of metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from diabetic foot ulcer patients

Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 2011

Metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa strains have been reported to be an... more Metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa strains have been reported to be an important cause of nosocomial infections. There is not enough information from India regarding their prevalence in diabetic foot ulcer (DFU) patients. The present study was undertaken over a period of two year from December 2008 to March 2011 to study the incidence of MBL producing P. aeruginosa isolated from 162 DFU patients with various grades of ulcer (Texas classification). Forty isolates of P. aeruginosa were obtained from patients. These isolates were subjected to susceptibility testing to anti-pseudomonal drugs as per Clinical Laboratory and Standards Institute (CLSI) guidelines, and were further screened for the production of MBL by disc potentiation testing using ethylenediaminetetraacetic acid (EDTA)-impregnated imipenem and meropenem discs. Of the 40 isolates of P. aeruginosa, 22 (55%) isolates were found resistant to carbapenems (imipenem) and 18 (81.1%) were found to be MBL producers using imipenem+(EDTA) and 15(68.1%) by meropenem+EDTA. This rapid dissemination of MBL producers is worrisome and necessitates the implementation of not just surveillance studies but also proper and judicious selection of antibiotics, especially carbapenems.

Research paper thumbnail of Bacteriological and Molecular Characterization of Diarrheagenic Escherichia Coli Pathotypes From Children in Duhok City, Iraq

Science Journal of University of Zakho, 2020

Background: A variety of diarrheagenic E coli (DEC) are responsible for causing different types o... more Background: A variety of diarrheagenic E coli (DEC) are responsible for causing different types of diarrhea in children especially in developing courtiers. Objectives: This study was primarily aimed to isolate and bacteriological characterizing of E coli from diarrheic infant stool and to investigate their antibiotic resistance patterns and then using molecular identification of DEC pathotypes for better discrimination. Methods: Total of 400 fresh stools specimens were collected from children with diarrhea in Heevi Hospital in Duhok city, Iraq. The samples were cultured on selective media such as (MacConkey and MacConkey sorbitol agar). Colonies were identified through biochemical reaction and VITEK 2 system and then antibiotic susceptibility profiles were determined. Results: A total of 349(87.2%) samples were yielded positive for growth of E coli. Out of these, 50 phenotypically-identified E coli were then subjected to PCR assay targeting certain virulence factors (alt, eae, sxt1 ...

Research paper thumbnail of Les fortifications de Jérusalem à l'époque de la Royauté de David et de Salomon

Research paper thumbnail of Carrier state of methicillin-resistant/sensitive Staphylococcus aureus in nasal and skin among physical education students, Duhok University, Iraq

Diagnostic and Therapeutic Study, 2013

In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially thos... more In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially those involved in athletic activities. (CA-MRSA) infections have become an increasing problem in athletics as well as in the general population during recent years. This study purposed to research MRSA/MSSA Staphylococcus aureus carriage in both the nose and skin and evaluation of their antibiotic susceptibility tests among students in physical education, Duhok University, Iraq. For this purpose, a total of 266 swabs that distributed between 133 nasal swabs and 133 skin swabs were taken from students between Marches to May 2013. The swab samples were examined through microbiological methods. All the isolates were tested for antimicrobial susceptibility by the disk diffusion technique according to the Clinical and Laboratory Standards Institute guidelines on Muller Hinton Agar. The prevalence of Staphylococcus aureus was 52(19.5%) over total number (266|) of nasal and skin swabs examined, of them 36(69%) and 16(31%) were methicillin-resistant S aureus MRSA and methicillin-sensitive S aureus MSSA, respectively. where, high prevalence of MRSA were recorded among nasal samples. MRSA expressed high percentage of resistance collectively to all antibiotics used. Generally, methicillin-sensitive S aureus MSSA exhibited low percentage of resistance collectively to all antibiotics used. This study highlighted that students submitted of Physical Education College might have been contaminated with MSSA/MRSA in their nose and skin during their study phase. That MSSA/MRSA being an important pathogen is seen in students taking a physical education.

Research paper thumbnail of Incidence and Resistotyping Profiles of Bacillus subtilis Isolated from Azadi Teaching Hospital in Duhok City, Iraq

Materia Socio Medica, 2012

Bacillus subtilis are opportunistic, spore forming bacteria, common soil inhabitants, and may fre... more Bacillus subtilis are opportunistic, spore forming bacteria, common soil inhabitants, and may frequently contaminate foods and widely distributed in hospital environments. This paper purposes to find out the incidence of Bacillus subtilis from various sources and locations at Azadi Teaching Hospital in Duhok city, Iraq. The susceptibility test and resistotyping (antibiotypes) profile of isolates were also studied. Samples, using sterile cotton swabs, were collected from various sources and locations and plated on Blood agar, chocolate agar and MacConkey agar. The recovered isolates were identified by routine procedures. Antibiotics susceptibility and resistant profiles to eight selected antibiotics were performed by the disc diffusion method using Mueller-Hinton agar. Out of the 128 samples collected 84 samples were yielded bacterial growth, of them 31(24.2%) were Bacillus subtilis. Moreover; other bacterial groups were isolated and identified. The susceptibility test of Bacillus subtilis isolates; the organism exhibited high susceptibility to gentamicin (96.7%) and ciprofloxacin (93.5%), while cephalexin (25.8%), cefotaxime (19.3%) and ampicillin (16.2%) demonstrated the lowest susceptibility rate. Resistotyping (antibiotypes) profiles for the isolates of Bacillus subtilis were determined. Out of 31 isolates, 22 of them were multiple resistant and belonged to 3 resistotype patterns; resistotype 1 was predominant among isolates. This study shows that there is an increased rate of incidence of Bacillus subtilis in hospital environments and some of these isolates were multi-drug resistant and showed different resistotyping profiles.

Research paper thumbnail of Laboratory evaluation of urine culture and drug resistance in outpatients clinically suspected of urinary tract infections Dr. Najim A. Yassin, Ph.D *

Rawal Medical Journal, 2012

This paper purpose to identify common bacterial etiologic agents causing urinary tract infection ... more This paper purpose to identify common bacterial etiologic agents causing urinary tract infection among outpatients with gender and age distribution visiting Azadi teaching hospital in Duhok city, Iraq. Of the 223 urine specimens cultured, 126 (56.5%) had a positive urine culture, of them 111(88%) female and 15(12%) male. Infections rate were higher among those aged 20-30 years in both gender. Escherichia coli was found to be the most frequent causative agent of UTIs (51.6%) followed by Enterococcus faecalis (27.8.3%), Klebsiella pneumonia (9.5%), Enterobacter aeruginosa (4.8%), Staphylococcus aureus (3.9%) and Pseudomonas aeruginosa (2.4%) of the cases. Escherichia coli showed variable susceptibility rate to different antibiotics as 90.5%, 84.7%, 71%, 46.2%, 43.1%, 43.1%, 41.6%, 35.4%, 33.9% and 21.6% of the isolates were found to be susceptibility to amikacin, nitrofuratoin, rifampicin, nalidixic acid, trimethoprim, gentamicin, ciprofloxacin, cephalothin, cefotaxime and amoxicillin, respectively. A total of 5 different resistotype patterns were obtained with Escherichia coli isolates; common resistotype was 1. This study shows increased rate of urinary tract infections in female than male population in age groups of 20+ and Escherichia coli were the most frequent causative agent and showed multi-drug resistance with different resistotyping patterns.

Research paper thumbnail of Study of Predictive Value of Pediatric Risk of Mortality (PRISM) Score in Children with End Stage Liver Disease and Fulminant Hepatic Failure

Indian Journal of Pediatrics - INDIAN J PEDIATR, 2011

Objective To evaluate the use of the PRISM score as a predictor of outcome in patients with end ... more Objective To evaluate the use of the PRISM score as a predictor of outcome in patients with end stage liver disease (ESLD) and fulminant hepatic failure (FHF). Methods The present study included 30 patients with ESLD and FHF, with ages ranging from 2 to 108 months, who were admitted to the Emergency room (ER) and the Pediatric Hepatology Unit at Cairo University Children’s Hospital (tertiary referral hospital) over a six-month-period from May through October 2008. Survivors were followed up for 6 months. Two other scores were also calculated, the PELD score and the Child-Pugh score. The outcome was defined as survivors and deceased. Results Deceased patients as compared with survivors were significantly younger (median age 7 vs. 24 months, p = 0.003). A ROC curve was constructed for the PRISM score, the predicted death rate (PDR) and the PELD score in the 30 patients. PRISM score was significantly associated with mortality (p = 0.04). The best cut off value was 9.5 (70.6% sensiti...

Research paper thumbnail of Honey as therapeutic agent for infectious diseases

Diagnostic and Therapeutic Study, 2013

Research paper thumbnail of CARRIER STATE OF METHICILLIN – RESISTANT/SENSITIVE STAPHYLOCOCCUS AUREUS IN NSAL AND SKIN AMONG PHYSICAL EDUCATION STUDENTS, DUHOK UNIVERSITY, IRAQ

In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially thos... more In fact, community-associated MRSA (CA-MRSA) now threatens the young and healthy; especially those involved in athletic activities. (CA-MRSA) infections have become an increasing problem in athletics as well as in the general population during recent years. This study purposed to research MRSA/MSSA Staphylococcus aureus carriage in both the nose and skin and evaluation of their antibiotic susceptibility tests among students in physical education, Duhok University, Iraq. For this purpose, a total of 266 swabs that distributed between 133 nasal swabs and 133 skin swabs were taken from students between Marches to May 2013. The swab samples were examined through microbiological methods. All the isolates were tested for antimicrobial susceptibility by the disk diffusion technique according to the Clinical and Laboratory Standards Institute guidelines on Muller Hinton Agar. The prevalence of Staphylococcus aureus was 52(19.5%) over total number (266|) of nasal and skin swabs examined, of them 36(69%) and 16(31%) were methicillin-resistant S aureus MRSA and methicillin-sensitive S aureus MSSA, respectively. where, high prevalence of MRSA were recorded among nasal samples. MRSA expressed high percentage of resistance collectively to all antibiotics used. Generally, methicillin-sensitive S aureus MSSA exhibited low percentage of resistance collectively to all antibiotics used. This study highlighted that students submitted of Physical Education College might have been contaminated with MSSA/MRSA in their nose and skin during their study phase. That MSSA/MRSA being an important pathogen is seen in students taking a physical education.