Yen Ning Chai - Academia.edu (original) (raw)

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Papers by Yen Ning Chai

Research paper thumbnail of Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops

Frontiers in Microbiology

Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, ... more Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was determined by quantitative polymerase chain reaction (qPCR). Two seed coating methods were also assessed in the field to test the reproducibility of the greenhouse results under non-sterile conditions. In the greenhouse seed coating methods were more successful in delivering the Gram-positive inoculant (Terrabacter sp.) wh...

Research paper thumbnail of The effects of soil depth on the structure of microbial communities in agricultural soils in Iowa, USA

Research paper thumbnail of Assessment of Bacterial Inoculant Delivery Methods for Cereal Crops

Frontiers in Microbiology

Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, ... more Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was determined by quantitative polymerase chain reaction (qPCR). Two seed coating methods were also assessed in the field to test the reproducibility of the greenhouse results under non-sterile conditions. In the greenhouse seed coating methods were more successful in delivering the Gram-positive inoculant (Terrabacter sp.) wh...

Research paper thumbnail of The effects of soil depth on the structure of microbial communities in agricultural soils in Iowa, USA

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