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Papers by Yesenia Guadalupe Contreras Magallanes

Research paper thumbnail of Vaccination Strategies in a Potential Use of the Vaccine against Bovine Tuberculosis in Infected Herds

Animals, Dec 1, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Descriptive and Time-Series Analysis of Rabies in Different Animal Species in Mexico

Frontiers in Veterinary Science

The spatio-temporal epidemiology of rabies has related the influence of environmental factors and... more The spatio-temporal epidemiology of rabies has related the influence of environmental factors and anthropogenic changes on the movements of the hematophagous bat Desmodus rotundus. In Mexico, D. rotundus is the main transmitter of the rabies virus for different livestock species, modifying annually the fluctuation of the number of cases of rabies and its dissemination in subtropical areas and regions considered free of the disease. The purpose of this study was to perform a descriptive analysis of the distribution of cases of rabies in Mexico, and to perform a time-series analysis to evaluate stationarity and to predict the number of cases for the following year. A total of 3,469 cases were reported in the period of interest, of which the 89.1% occurred in cattle, 4.3% in horses, 1.5% in sheep, 0.6% in goats, 0.01% in pig, 3.1% in vampire bats, 0.3% in cervids, 0.2% in skunks, 0.1% in insectivorous bats, 0.1% in foxes, 0.1% in buffaloes, and 0.02% in coatis; 0.5% were not identified...

Research paper thumbnail of Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model

Animals : an Open Access Journal from MDPI, 2021

Simple Summary Bovine tuberculosis is a disease that affects cattle and other animal species worl... more Simple Summary Bovine tuberculosis is a disease that affects cattle and other animal species worldwide and represents a risk to public health. Even though there is a vaccine that has been used to control tuberculosis in humans for almost 100 years, up to now, it has not been used in animals. The reason is that vaccination interferes with the tuberculin test, the current test to diagnose tuberculosis in the field, and shows an inconsistent efficacy in animals. Recent studies report that prime vaccinating with BCG and boosting with proteins vaccinations perform better. In addition, there are reports that some polymers increase the immune response against various infectious diseases; therefore, testing a vaccine formula with polymers sounds like a wise thing to do. In this study, we showed that priming with BCG and boosting with a culture filtrate protein, alone or in combination with a polymer, the number of animals with lesions, the number of lesions per animal, and the size of the l...

Research paper thumbnail of Evaluación de los factores promotores de resucitación como impulsores del crecimiento de Mycobacterium bovis en cultivos

En este trabajo se evaluo el efecto de las proteinas recombinantes RipA y RpfB sobre el crecimien... more En este trabajo se evaluo el efecto de las proteinas recombinantes RipA y RpfB sobre el crecimiento de bacterias dormantes y activas de Mycobacterium bovis AN5. Se emplearon cuatro concentraciones de proteina total: 15, 30, 60 y 120 µg, para RipA y RpfB, y una combinacion RipA + RpfB; en medios Middlebrook 7H9 y7H10. Como control positivo se utilizo filtrado de cultivo de Mycobacterium bovis (F.C. 120 µg) y un control negativo (sin proteina). Para hacer la reactivacion, las bacterias fueron previamente inducidas a dormancia poniendolas cuatro meses en medio minimo Sauton, donde se les privo de nutrientes y oxigeno. Para confirmar el estado en que se encontraban las bacterias, el cultivo se evaluo cada 15 dias para medir la densidad optica (D.O. 600nm), tambien por conteo de bacterias totales en camara de Neubauer y conteo de UFC en medio solido. Posteriormente se adicionaron las proteinas en los medios solidos y liquidos con bacterias dormantes o activas. En los medios solidos se ev...

Research paper thumbnail of Vaccination Strategies in a Potential Use of the Vaccine against Bovine Tuberculosis in Infected Herds

Animals, Dec 1, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Descriptive and Time-Series Analysis of Rabies in Different Animal Species in Mexico

Frontiers in Veterinary Science

The spatio-temporal epidemiology of rabies has related the influence of environmental factors and... more The spatio-temporal epidemiology of rabies has related the influence of environmental factors and anthropogenic changes on the movements of the hematophagous bat Desmodus rotundus. In Mexico, D. rotundus is the main transmitter of the rabies virus for different livestock species, modifying annually the fluctuation of the number of cases of rabies and its dissemination in subtropical areas and regions considered free of the disease. The purpose of this study was to perform a descriptive analysis of the distribution of cases of rabies in Mexico, and to perform a time-series analysis to evaluate stationarity and to predict the number of cases for the following year. A total of 3,469 cases were reported in the period of interest, of which the 89.1% occurred in cattle, 4.3% in horses, 1.5% in sheep, 0.6% in goats, 0.01% in pig, 3.1% in vampire bats, 0.3% in cervids, 0.2% in skunks, 0.1% in insectivorous bats, 0.1% in foxes, 0.1% in buffaloes, and 0.02% in coatis; 0.5% were not identified...

Research paper thumbnail of Prime Vaccination with Chitosan-Coated Phipps BCG and Boosting with CFP-PLGA against Tuberculosis in a Goat Model

Animals : an Open Access Journal from MDPI, 2021

Simple Summary Bovine tuberculosis is a disease that affects cattle and other animal species worl... more Simple Summary Bovine tuberculosis is a disease that affects cattle and other animal species worldwide and represents a risk to public health. Even though there is a vaccine that has been used to control tuberculosis in humans for almost 100 years, up to now, it has not been used in animals. The reason is that vaccination interferes with the tuberculin test, the current test to diagnose tuberculosis in the field, and shows an inconsistent efficacy in animals. Recent studies report that prime vaccinating with BCG and boosting with proteins vaccinations perform better. In addition, there are reports that some polymers increase the immune response against various infectious diseases; therefore, testing a vaccine formula with polymers sounds like a wise thing to do. In this study, we showed that priming with BCG and boosting with a culture filtrate protein, alone or in combination with a polymer, the number of animals with lesions, the number of lesions per animal, and the size of the l...

Research paper thumbnail of Evaluación de los factores promotores de resucitación como impulsores del crecimiento de Mycobacterium bovis en cultivos

En este trabajo se evaluo el efecto de las proteinas recombinantes RipA y RpfB sobre el crecimien... more En este trabajo se evaluo el efecto de las proteinas recombinantes RipA y RpfB sobre el crecimiento de bacterias dormantes y activas de Mycobacterium bovis AN5. Se emplearon cuatro concentraciones de proteina total: 15, 30, 60 y 120 µg, para RipA y RpfB, y una combinacion RipA + RpfB; en medios Middlebrook 7H9 y7H10. Como control positivo se utilizo filtrado de cultivo de Mycobacterium bovis (F.C. 120 µg) y un control negativo (sin proteina). Para hacer la reactivacion, las bacterias fueron previamente inducidas a dormancia poniendolas cuatro meses en medio minimo Sauton, donde se les privo de nutrientes y oxigeno. Para confirmar el estado en que se encontraban las bacterias, el cultivo se evaluo cada 15 dias para medir la densidad optica (D.O. 600nm), tambien por conteo de bacterias totales en camara de Neubauer y conteo de UFC en medio solido. Posteriormente se adicionaron las proteinas en los medios solidos y liquidos con bacterias dormantes o activas. En los medios solidos se ev...