Ying Wang - Academia.edu (original) (raw)

Papers by Ying Wang

Science, 1999

After printing, free amino groups of the poly-L-lysine-coated sl~des were acetylated with 5.5 g o... more After printing, free amino groups of the poly-L-lysine-coated sl~des were acetylated with 5.5 g of succinic anhydride in 350 ml of borate-buffered I-methyl-2-pyrrolidinone (pH 8). 9. Bacteria listed above were grown in 7H9 broth with ADC enrichment, and DNA was extracted after 14 t o 21 days' growth as described [D, van Soolingen, P. W. Hermans, P. E, de Haas, D. R. Soll, J. D, van Embden, ] Clin. Microbial. 29, 2578Microbial. 29, (1991l. Whole genomic DNA from either H37Rv or BCC strain (2 lpg) was used as templates for direct incorporation of fluorescent nucleotide analogs (Cy3 and Cy5 dUTP, Amersham) by a randomly primed polymerization reaction, In brief, 50-1~1 labeling reactions contained 2 p g of template DNA, 5 1~1 of 10X buffer, 1.5 1~1 of fluorescent dUTP, 0.5 1~1 each of 5 m M dATP, dCTP, and dCTP, 1 pi of random hexamers and decamers, and 2 1~1 of Kleno~v (Escherichia coli DNA polymerase 3' t o 5'exo-, New England Biolabs) Labeled DNA8 from the strains being compared were mixed together to 14.75 1~1 total volume in a solution with final concentration of 4 X SSC buffer, tRNA (0.7 p g i l~l ) , and 0.3% SDS. The hybridization mixture was denatured at 98°C for 2 min, cooled t o 65"C, applied t o the microarray, and covered with a 22-mm2 cover slip. The slide was then placed in a waterproof hybridization chamber for hybridization in a 65°C water bath for 3 hours After hybridization, slides were washed 2 min in 1X SSC buffer with 0.06% SDS followed by 2 min in 0.06X SSC buffer. 10. S. Sreevatsan et a/.

Science, 1999

After printing, free amino groups of the poly-L-lysine-coated sl~des were acetylated with 5.5 g o... more After printing, free amino groups of the poly-L-lysine-coated sl~des were acetylated with 5.5 g of succinic anhydride in 350 ml of borate-buffered I-methyl-2-pyrrolidinone (pH 8). 9. Bacteria listed above were grown in 7H9 broth with ADC enrichment, and DNA was extracted after 14 t o 21 days' growth as described [D, van Soolingen, P. W. Hermans, P. E, de Haas, D. R. Soll, J. D, van Embden, ] Clin. Microbial. 29, 2578Microbial. 29, (1991l. Whole genomic DNA from either H37Rv or BCC strain (2 lpg) was used as templates for direct incorporation of fluorescent nucleotide analogs (Cy3 and Cy5 dUTP, Amersham) by a randomly primed polymerization reaction, In brief, 50-1~1 labeling reactions contained 2 p g of template DNA, 5 1~1 of 10X buffer, 1.5 1~1 of fluorescent dUTP, 0.5 1~1 each of 5 m M dATP, dCTP, and dCTP, 1 pi of random hexamers and decamers, and 2 1~1 of Kleno~v (Escherichia coli DNA polymerase 3' t o 5'exo-, New England Biolabs) Labeled DNA8 from the strains being compared were mixed together to 14.75 1~1 total volume in a solution with final concentration of 4 X SSC buffer, tRNA (0.7 p g i l~l ) , and 0.3% SDS. The hybridization mixture was denatured at 98°C for 2 min, cooled t o 65"C, applied t o the microarray, and covered with a 22-mm2 cover slip. The slide was then placed in a waterproof hybridization chamber for hybridization in a 65°C water bath for 3 hours After hybridization, slides were washed 2 min in 1X SSC buffer with 0.06% SDS followed by 2 min in 0.06X SSC buffer. 10. S. Sreevatsan et a/.