Yousef Nami - Academia.edu (original) (raw)

Papers by Yousef Nami

Frontiers in Microbiology, Dec 5, 2023

DOAJ (DOAJ: Directory of Open Access Journals), Nov 1, 2021

The growth of contaminating microorganisms in food products causes serious diseases in humans, an... more The growth of contaminating microorganisms in food products causes serious diseases in humans, and the use of lactic acid bacteria to control these contaminants is a promising and developing method. In this study, lactic acid bacteria were isolated from traditional yogurts and a total of 140 isolates were isolated. The probiotic potential of these lactic acid bacteria including resistance to acidic conditions, resistance to bile salts and sensitivity to ten important antibiotics were investigated and then bacteria with probiotic potential were used to inhibit food pathogens. The measured halo diameters of the bacterial medium were measured and recorded, and the antimicrobial properties of the strains were compared. ARDRA technique was also used to identify the strains. The results showed that all strains have excellent tolerance to pH 2.5 and bile salt of 0.3% and isolates Numbers 12, 100 and 102 are recognized as the most resistant strains against studied food pathogens. Findings from this study showed that yogurt samples are an important source for the production of bacteria with probiotic potential with antimicrobial activity and can be used against food pathogens.

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In order to develop molecular markers based on Expressed Sequence Tag (EST) linked to Fusarium He... more In order to develop molecular markers based on Expressed Sequence Tag (EST) linked to Fusarium Head Blight (FHB) resistance genes in wheat, the biological function of 8660 ESTs deposited in data bank were studied following which 260 ESTs with identified biological ...

Summary Among probiotics, acetic acid bacteria (AAB) can be used as preservative agents because o... more Summary Among probiotics, acetic acid bacteria (AAB) can be used as preservative agents because of their high fermentation and acidification activities. This study aimed to isolate, identify and biologically characterise Acetobacter strains from traditional Iranian dairy products. Acetobacter strains were identified by catalase assay, Gram staining, and combined repetitive sequence-based PCR [(GTG)5-PCR fingerprints] and 16S rDNA gene sequencing. We identified eight strains belonging to four species, including Acetobacter aceti, Acetobacter indonesiensis, Acetobacter cibinongensis and Acetobacter syzygii. The molecular techniques could be used as an effective and rapid alternative tool to identify and characterise dairy-associated AAB. Primary probiotic assessments, including low pH and high bile salt tolerance tests, antagonistic activity test against pathogens, and antibiotic susceptibility confirmed the probiotic properties of these AAB, particularly A. cibinongensis 34L strain, ...

The streptococcal disease has been associated with serious mortality and significant global econo... more The streptococcal disease has been associated with serious mortality and significant global economic loss in the tilapia farming industry. The overall goal of this work was to test herbal hydrogels based on encapsulated Enterococcus faecium ABRIINW.N7 for potential probiotic anti‐microbial activity against Streptococcus iniae in red hybrid tilapia.

Journal of Medical Microbiology

Genetic and environmental factors can affect intestinal microbiome and microbial metabolome. Amon... more Genetic and environmental factors can affect intestinal microbiome and microbial metabolome. Among these environmental factors, the consumption of antibiotics can significantly change the intestinal microbiome of individuals and consequently affect the corresponding metagenome. The term "probiotics" is related to preventive medicine rather than therapeutic procedures and is thus considered the opposite of antibiotics. This review discusses the challenges between these contradictory terms in terms of the following points: I) antibiotic resistance, relationship between antibiotic consumption and microbiome diversity reduction, antibiotic effect on metagenome lesion, and disease associated with antibiotics; and II) probiotics as living drugs, probiotic effect on epigenetically alterations, and gut microbiome relevance to hygiene indulgence. The intestinal microbiome is more specific for individuals and may be altered by environmental alterations and the occurrence of diseases...

Journal of applied microbiology, 2015

Investigation on the use of herbal-based biopolymers for probiotic-Lactobacillus plantarum 15HN-e... more Investigation on the use of herbal-based biopolymers for probiotic-Lactobacillus plantarum 15HN-encapsulation is presented. The objectives are to enhance its oral delivery, colonic release and survival rate of these probiotic cultures in gastrointestinal environment. Nine types of herbal-based polymers blend with different concentration of alginate alone or mixed with psyllium and fenugreek was used as candidate for encapsulation matrix by applying a simple extrusion method. All the blend formulations recorded high encapsulation efficiency at value >98%. The survival rate of viable probiotic cells under both low pH and high bile salt conditions was also high with value above 80% in 2% (w/v) alginate, alginate+psyllium (1·5 + 0·5%) blend and alginate+fenugreek (1·5 + 0·5%) blend as compared to other polymer formulations and nonencapsulated cells. Their release occurred after 2 h in colonic condition and sustained until the 12th hour incubation period. A value added prebiotic effec...

Journal of medical microbiology, 2014

Forty-five lactic acid bacteria (LAB) were isolated from the vaginal specimens of healthy fertile... more Forty-five lactic acid bacteria (LAB) were isolated from the vaginal specimens of healthy fertile women, and the identities of the bacteria were confirmed by sequencing of their 16S rDNA genes. Among these bacteria, only four isolates were able to resist and survive in low pH, bile salts and simulated in vitro digestion conditions. Lactococcus lactis 2HL, Enterococcus durans 6HL, Lactobacillus acidophilus 36YL and Lactobacillus plantarum 5BL showed the best resistance to these conditions. These strains were evaluated further to assess their ability to adhere to human intestinal Caco-2 cells. Lactococcus lactis 2HL and E. durans 6HL were the most adherent strains. In vitro tests under neutralized pH proved the antimicrobial activity of both strains. Results revealed that the growth of Escherichia coli O26, Staphylococcus aureus and Shigella flexneri was suppressed by both LAB strains. The antibiotic susceptibility tests showed that these strains were sensitive to all nine antibiotics...

Journal of Applied Microbiology, 2014

Journal of Medical Microbiology, 2014