Yudi Purnomo - Academia.edu (original) (raw)

Papers by Yudi Purnomo

Research journal of pharmacy and technology, Nov 30, 2023

Jurnal kesehatan Islam, Sep 24, 2020

Jurnal Wiyata: Penelitian Sains dan Kesehatan, Aug 26, 2022

Journal of Dedicators Community

Pandemi Coronavirus disease atau yang biasa disebut COVID-19 menimbulkan dampak di berbagai sekto... more Pandemi Coronavirus disease atau yang biasa disebut COVID-19 menimbulkan dampak di berbagai sektor kehidupan masyarakat. Perlu upaya serius untuk meningkatkan ketahanan tubuh dengan memanfaatkan ramuan herbal yang banyak kita jumpai di kebun tanaman obat keluarga. Program edukasi kader penggerak PKK tentang ramuan herbal pencegahan COVID-19 ini dilaksanakan di Kota Batu propinsi Jawa Timur. Penyampaian program melalui ceramah, pelatihan dan FGD UMKM Griya Jamu Siti Ara Kota Batu pada Desember 2021. Edukasi interaktif dengan memberikan materi berupa ceramah tentang khasiat jamu/herbal dan pelatihan keterampilan meracik jamu/herbal. Analisis uji korelasi Kendall’s tau-b menunjukkan adanya hubungan karakteristik sosioekonomi dan pendidikan dengan persepsi COVID-19. Uji korelasi Pearson menunjukkan tidak terdapat hubungan paparan COVID-19 dengan persepsi peserta engan COVID-19. Danlagi, uji Chi-Square menunjukkan tidak terdapat hubungan karakteristik sosioekonomi dan pendidikan peserta...

Kuwait Journal of Science

INDONESIAN JOURNAL OF PHARMACY, Sep 30, 2022

Pulutan (Urena lobata L.) is a medicinal plant that has been traditionally used to treat fever, i... more Pulutan (Urena lobata L.) is a medicinal plant that has been traditionally used to treat fever, infection, and inflammation. However, preclinical studies on its activity as an analgesic and anti-inflammatory agent are still limited. The present study was aimed at investigating the analgesic and anti-inflammatory activities of U. lobata leaf extracts, as well as predicting the biological properties of the active components. Eight (two control and six test) groups of male Wistar rats were used in this study. Water and ethanol were used to extract the leaves of U. lobata, and each prepared in doses of 125, 250, and 500 mg/kg body weight (BW) for the treatments. The antiinflammatory and analgesic activities of the extracts were evaluated. Following active compound identification using UHPLC-MS/MS, several compounds were analyzed for drug-likeness and ADME-T properties. The results revealed that both aqueous and ethanolic extracts of U. lobata at 125 and 250 mg/kg BW significantly (p < 0.05) inhibited paw edema with an area under curve (AUC) volume of 10 and 5%, respectively, compared to the control group. For analgesic activity, the aqueous extract of U. lobata was able to significantly (p<0.05) increase the AUC for pain threshold by 30-100 % compared to the control group. However, the activity of the ethanolic extract was lower than the aqueous extract. The drug-likeness analysis indicated that all the phytoconstituents were within the range established by Lipinski's rule of five. Stigmasterol, ß-sitosterol, and mangiferin were predicted to have better ADME-T properties than that of the other compounds.

Annals of Neurology, 2001

Postnatal development of the mouse uterus involves differentiation and development of the endomet... more Postnatal development of the mouse uterus involves differentiation and development of the endometrial glands as well as the myometrium. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are involved in extracellular matrix breakdown and morphogenesis of many epitheliomesenchymal organs. As a first step to understanding their roles in postnatal mouse uterine development, MMPs and TIMPs found to be expressed in the neonatal mouse uterus by microarray analysis were localized by in situ hybridization. The MMP-2 mRNA was detected only in the uterine stroma, whereas the MMP-10 mRNA was present only in the uterine epithelium from Postnatal Day (PND) 3 to PND 9. All other MMPs (MMP-11, MMP-14, and MMP-23) as well as TIMP-1, TIMP-2, and TIMP-3 were detected in both epithelial and stromal cells of the endometrium, but not in the myometrium. Uterine extracts were then analyzed by gelatin and casein gel zymography to detect active gelatinases and stromelysins, respectively. Five major gelatinase bands of activity were detected and inhibited by the MMP inhibitors, EDTA or 1,10-phenanthroline, but not by PMSF, a serine protease inhibitor. Western blot analysis confirmed the presence of MMP-2 and MMP-9 proteins in the uterus. Immunoreactive MMP-9 protein was detected only in the endometrial stroma, whereas immunoreactive MMP-2 protein was detected in both the stroma and epithelium of the uterus. Casein zymography detected three major bands of activity (ϳ54, 63, and 80 kDa) that were inhibited by the serine protease inhibitor, PMSF, but not by the MMP inhibitors, EDTA or 1,10-phenanthroline, suggesting that they were serine proteases. These results support the hypothesis that MMPs and TIMPs regulate postnatal development of the mouse uterus.

Journal of Traditional and Complementary Medicine, Jul 1, 2017

This study aims to determine the incretin effects of Urena lobata leaves extract on the structure... more This study aims to determine the incretin effects of Urena lobata leaves extract on the structure and function of rats islet b-cells. This study utilizes male Sprague-Dawley rats divided into 2 control group and 3 test group (n ¼ 5). Diabetic rats were induced with High Fructose Diet (HFD) and single dose intraperitoneal streptozotocin 25 mg/kg bw. Aqueous leaves extract of U. lobata was prepared by decoction methods and administrated orally with doses of 250, 500, and 1000 mg/kg bw for 4 weeks then incretin effect was evaluated by measuring serum GLP-1, insulin, and blood glucose levels. Histology of islet b-cells was evaluated using photomicroscopy by analyzing size, shape, and number. Data were analyzed using ANOVA test followed by LSD test and p 0.05 is considered significant. Oral administration of aqueous extract U. lobata leaves at doses of 250, 500, and 1000 mg/kg body weight were able to prolong GLP-1 bioavailability by 3-fold, 5-fold, and 7-fold respectively when compared to the diabetic group whereas blood glucose level were decreased about 30%, 35%, and 40% respectively (p < 0.05). Extract at doses of 500 and 1000 mg/kg bw also increased insulin level by 4-fold and 8-fold respectively compared to the diabetic group and the islet b-cells were repaired. The active compound in U. lobata leaves extract are suggested to prevent degradation of GLP-1 by inhibition of DPP-4 activity. Aqueous extract of U. lobata also improved the structure and function of islet b-cells by increasing of GLP-1 bioavailability.

Diabetes Research and Clinical Practice, Nov 1, 2014

Background: Nowdays, incretin hormone like Glucagon Like Peptide-1 (GLP-1) become target of a new... more Background: Nowdays, incretin hormone like Glucagon Like Peptide-1 (GLP-1) become target of a new treatment for type 2 diabetes, however this hormone is metabolized by Dipeptidyl Peptidase IV (DPP-IV) quickly into inactive form. The inhibition of DPP-IV can increase GLP-1 bioavaibility and regulate blood glucose level therefore it would be beneficial in the treatment of type 2 diabetes. In the other hand, the long term use of DPP-IV inhibitor has not been obtained the complete data especially about the safety. It induce search of natural compounds that can inhibit DPP-IV with less side effect. Urena lobata and Tithonia diversifolia are plant growing in Indonesia and has been used to cure many diseases. The aim of the study was to evaluate anti diabetic potency of U. lobata and T. diversifolia leaf extract on DPP-IV inhibitory activity. Method: U. lobata and T. diversifolia leaf were extracted in the ethanol solvent then DPP-IV inhibitory activity tested in-vitro. Gly-prop -nitroanilide (GPPN) was used as substrate of DPP-IV and Vildagliptin as a reference standard. p-nitroanilide as product of reaction between GPPN and DPP-IV was observed with microplate reader with wavelength 405 nm. All data are expressed as the mean ± SD and the IC-50 value was determined by linear regression curve. Result: The ethanolic extract of U. lobata showed DPP-IV inhibitory activity stronger than T. diversifolia with an Inhibitory Concentration-50 (IC-50) value of 2,355.43 and 15,385.27 mg/ml respectively. The results confirm that U. lobata leaf extract has more inhibitory effect on DPP-IV related to active compound in this extract such as fitosterol and flavonoid groups. The inhibition of DPP-IV will increase bioavailability of incretin hormone that contribute to regulate blood glucose level through stimulation of insulin secretion, b cell proliferation and inhibition of glucagon. It might be potential to be a candidate of fitotherapy for the diabetes mellitus type 2. Conclusion: U. lobata leaf extract showed DPP-IV inhibitory activity stronger than T. diversifolia

Medicinal Plants - International Journal of Phytomedicines and Related Industries, 2018

This study was aimed to compare the anti diabetic effect of Urena lobata leaf extract through DPP... more This study was aimed to compare the anti diabetic effect of Urena lobata leaf extract through DPP-4 inhibitory activity by in vitro and in vivo. Urena lobata leaf was extracted in ethanol and hot water to evaluate its activity on DPP-4 both of in vitro and in vivo. In vitro test using Gly-pro-p-nitroanilide (GPPN) as substrate of DPP-4 and the reactions product of them was observed by microplate reader at λ=405 nm furthermore the IC50 value was determined. In vivo study utilize an animal model of diabetes with 2 control groups and 6 test groups (n=4), in which DPP-4 level, GLP-1 level and AUC of blood glucose were examined after extract administration. The in vitro DPP-4 inhibitory activity of ethanolic extract of U. lobata is higher than water extract with the IC50 value of 1654, 64 and 6489, 88 μg/ml respectively. However, the water extract of U. lobata exhibits stronger decrease DPP-4 level (60–70%) compared to ethanolic extract (40–60%) in vivo study as well as the AUC of blood glucose were reduced by 50–60% and 20–50%, respectively. Meanwhile, GLP-1 level could be retained more by the water extract of U. lobata administration (3–7 fold) compared to ethanolic extract (25 fold) due to the reducing of DPP-4 activity.

Asian pacific Journal of Tropical Biomedicine, Aug 1, 2015

To evaluate the anti-diabetic potential of leaf extract from Urena lobata (U. lobata) through dip... more To evaluate the anti-diabetic potential of leaf extract from Urena lobata (U. lobata) through dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPP-IV inhibitor was tested by in vitro study using gly-prop -nitroanilide as substrat of DPP-IV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with l = 405 nm. All data were expressed as mean ± SD and the IC 50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC 50 values of 1 654.64 and 6 489.88 mg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC 50 value of 57.44 mg/mL. Based on in silico analysis, mangiferin, stigmasterol and b-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and b-sitosterol.

Research Journal of Pharmacy and Technology, Jan 27, 2023

Glucagon Like Peptide-1 (GLP-1) is one of incretin hormone which is proposed as a new therapy for... more Glucagon Like Peptide-1 (GLP-1) is one of incretin hormone which is proposed as a new therapy for type 2 diabetes (T2DM). However, this hormone is metabolized excessively by Dipeptidyl Peptidase IV (DPP-IV) into inactive form. The inhibition of DPP-IV can prolong GLP-1 bioavailability for regulating blood glucose level on T2DM. Urena lobata is a plant which has been used to cure T2DM empirically but the inhibitory activity on DPP-IV has not been tested. The aim of the study was to evaluate anti diabetic effect of U. lobata leaf extract through DPP-IV inhibition. Urena lobata leaf was extracted in ethanol solvent and hot water then evaporated till pasta form. The object study was used animal model of T2DM which divided into 2 control group and 6 test group (n=4) and then DPP-IV level, GLP-1, insulin and blood glucose AUC were examined after supplemented U. lobata leaf extract. All data are expressed as the mean ± SD and analyzed with one way anova and then continued with LSD and Dunn...

Journal of Traditional and Complementary Medicine, 2017

Research Journal of Pharmacy and Technology

Pulutan (Urena lobata) is one of medicinal plant used to treat some diseases traditionally and pr... more Pulutan (Urena lobata) is one of medicinal plant used to treat some diseases traditionally and pre-clinical studies have showed its efficacy. However, the study about its safety has not been evaluated completely. An acute toxicity test has to be performed in order to screen for its safety. The objective of study to determine the acute toxicity level of Urena lobata (U. lobata) leaf extract on embryo phase, juvenile and adult of zebrafish (Danio rerio) and the role of its active constituents through in silico methods. This was an experimental laboratory study using embryo, juvenile and adult of zebrafish (Danio rerio). The leaf of U. lobata was extracted by decoction methods and the extract was diluted from 12000 mg/L to 500 mg/L. The animals were exposed to the extracts for 96 hours. Toxicity level of herbs was defined using lethal concentration-50 (LC-50) obtained through linear regression. In silico study was performed using a web-based software application (iLAB ACD). The LC-50 v...

Backgound. iNOS enzyme was secreted by immunocompetent cells during pathogenesis of diseases invo... more Backgound. iNOS enzyme was secreted by immunocompetent cells during pathogenesis of diseases involving inflammation. The enzyme catalized a reaction resulting in nitric oxide radical (NO˙) in large amount. The radical reacts with superoxide (O2 ) to yield peroxynitrite ( ONOO ) which are highly reactive and toxic to the tissues. Kayumanis (C.burmanii) was considered rich with compounds overcoming iNOS secretion. This is the initial study of kayumanis potency in reducing iNOS expression. Diabetes is one of many diseases with high level of serum iNOS and was choosen for the study. Purpose. To study kayumanis potency in reducing serum iNOS of a diabetic conditions. Methode.5 groups of rats, each 5, four groups of which were intraperitoneally injected by aloxan at dose of 150 mg/Kg BW to induce hyperglycemia. The another group recieved no treatment. Three groups of diabetic rats were treated with C. Burmanii each day at dose of 0. 5 ml, 1 ml, and 2 ml respectively for 14 days. The remai...

IOP Conference Series: Materials Science and Engineering, 2020

This research is to evaluate whether Scurrula atropurpurea has a protective effect on the liver c... more This research is to evaluate whether Scurrula atropurpurea has a protective effect on the liver caused by its sub-chronic treatment. The rats consist of four groups: normotensive group and three groups administered methanolic extract of S. atropurpurea (MESA) at a dosage of 250; 500; and 1000 mg/kg BW. Serum liver and kidney function were assayed. Serum cholesterol was checked. Histopathology of the brain, liver, cardiac, lung, and kidney was identified using hematoxylin-eosin staining. The administration of MESA250, MESA500 and MESA1000 caused no differences among the groups compared with the control group. Structural evaluations on the brain and lungs showed that the MESA administration carried no changes. In liver and heart, MESA1000 led to repair tissue damage. But for the kidney, MESA250 was safe for the tissue. The results of this study revealed that the methanolic extract of S. atropurpurea at antihypertension dose is safe even when taken for a more extended period. At a high...

Diabetes Research and Clinical Practice, 2014