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Papers by Yuewei Wu-Fienberg

Research paper thumbnail of Characterization and Cost of Intravenous Drug Use–Associated Upper Extremity Infections Requiring Operative Treatment

Plastic & Reconstructive Surgery, 2021

Research paper thumbnail of Targeted Muscle Reinnervation for Partial Hand Amputation

Plastic and Reconstructive Surgery - Global Open, 2020

Research paper thumbnail of Abstract: Implications of Upper Eyelid Ptosis and Blepharodermatochalasis on Fixed Horizontal Forehead Lines

Plastic and Reconstructive Surgery Global Open, 2018

GOALS/PURPOSE: In this age of globalization, patients may seek to have their aesthetic surgeries ... more GOALS/PURPOSE: In this age of globalization, patients may seek to have their aesthetic surgeries performed in foreign countries anticipating significant cost-savings compared to perceived prohibitive out-of-pocket costs in the US. Given the transient nature of their stay in the country of surgery, post-operative follow-up is often sub-optimal. These patients frequently seek care in the US for complications, the burden falling on the US healthcare system. Non-standardization of aseptic surgical facilities and technique leads to an increased risk of infectious complications. Management of these complications represents a resource burden to the healthcare system and poses a clinical danger to the general population. Travel and surgery in uncontrolled settings, with their disparate macro-biomes, facilitates translocation of potentially dangerous infectious microbiota. This study investigated the microbiological data from a series of patients who sustained infectious complications from t...

Research paper thumbnail of An Alternative Dermal Template for Reconstruction of Complex Upper Extremity Wounds

Plastic and Reconstructive Surgery - Global Open, 2021

Research paper thumbnail of Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression

Biotechnology and Bioengineering, 2014

Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhan... more Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to reduced motor axons available to reinnervate muscle. To address this issue, we designed a cell-based GDNF expression system that can temporally regulate protein expression using an inducible gene excision mechanism to prevent entrapment at the site of expression. To design this system for regulation of GDNF expression, we transduced two lentiviral vectors, one containing a constitutively active GDNF transgene flanked by two loxP sites, and the other containing a tetracycline-inducible cre transgene along with its constitutively active transactivator, into Schwann cells (SCs). These SCs over-express GDNF, but expression can be suppressed through the administration of tetracycline family antibiotics, such as doxycycline. The engineered SCs produced significantly more GDNF as compared to untransduced controls, as measured by enzyme-linked immunosorbent assay (ELISA). Following doxycycline treatment, these SCs produced significantly lower levels of GDNF and induced less neurite extension as compared to untreated SCs. Engineered SCs treated with doxycycline showed a marked increase in Cre recombinase expression, as visualized by immunohistochemistry (IHC), providing evidence of a mechanism for the observed changes in GDNF expression levels and biological activity. This cell-based GDNF expression system could have potential for future in vivo studies to provide a temporally controlled GDNF source to promote axon growth.

Research paper thumbnail of Psychiatric Disease after Isolated Traumatic Upper Extremity Amputation

Journal of Hand and Microsurgery

Introduction Psychiatric disease after traumatic limb loss impacts rehabilitation, prosthesis use... more Introduction Psychiatric disease after traumatic limb loss impacts rehabilitation, prosthesis use, and quality of life. The purpose of this study was to evaluate the prevalence of psychiatric disease in civilians after isolated, traumatic upper extremity amputation and determine if any risk factors are associated with developing psychiatric disease. Materials and Methods Demographics, time since injury, mechanism of injury, amputation level, hand affected (dominant vs. nondominant), Bureau of Workers’ Compensation (BWC) status, and prosthesis use were retrospectively reviewed for all patients treated from 2012 to 2017. For patients with an International Statistical Classification of Diseases and Related Health Problems, 10th revision (ICD-10) diagnosed psychiatric disease, the diagnosis and length of treatment were recorded. Patients were grouped by presence or absence of psychiatric diagnosis and data analysis was performed using descriptive statistics, Fisher’s exact test, and rel...

Research paper thumbnail of The Anterolateral Thigh-Medial Femoral Condyle Chimeric Flow-through Flap for Posttraumatic Wrist Arthrodesis

Techniques in Hand & Upper Extremity Surgery

Research paper thumbnail of Impact of interdisciplinary rounds in the spinal cord injury unit on relational coordination for patients with pressure injury: A pilot study

The Journal of Spinal Cord Medicine

Research paper thumbnail of The importance of surgical maneuvers during treatment of frontal migraines (site I): A prospective, randomized cohort study evaluating foraminotomy/fasciotomy, myectomy, and arterectomy

Journal of Plastic, Reconstructive & Aesthetic Surgery

Research paper thumbnail of Horizontal Forehead Lines: A Reflection of Eyelid Ptosis or Blepharodermachalasia

Aesthetic Plastic Surgery

Research paper thumbnail of Anatomical Look Into OnabotulinumtoxinA Injection for Chronic Migraine Headache

Regional Anesthesia and Pain Medicine

Research paper thumbnail of Repair of a Large, Full-Thickness Defect of the Lower Lip Under Local Anesthesia

Research paper thumbnail of Considering the needle length and Body Mass Index as factors that might increase the response rate to onabotulinumtoxinA in Chronic Migraine

Research paper thumbnail of Abstract 91

Plastic and Reconstructive Surgery, 2014

Research paper thumbnail of Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression

Biotechnology and Bioengineering, 2014

Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhan... more Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to reduced motor axons available to reinnervate muscle. To address this issue, we designed a cell-based GDNF expression system that can temporally regulate protein expression using an inducible gene excision mechanism to prevent entrapment at the site of expression. To design this system for regulation of GDNF expression, we transduced two lentiviral vectors, one containing a constitutively active GDNF transgene flanked by two loxP sites, and the other containing a tetracycline-inducible cre transgene along with its constitutively active transactivator, into Schwann cells (SCs). These SCs over-express GDNF, but expression can be suppressed through the administration of tetracycline family antibiotics, such as doxycycline. The engineered SCs produced significantly more GDNF as compared to untransduced controls, as measured by enzyme-linked immunosorbent assay (ELISA). Following doxycycline treatment, these SCs produced significantly lower levels of GDNF and induced less neurite extension as compared to untreated SCs. Engineered SCs treated with doxycycline showed a marked increase in Cre recombinase expression, as visualized by immunohistochemistry (IHC), providing evidence of a mechanism for the observed changes in GDNF expression levels and biological activity. This cell-based GDNF expression system could have potential for future in vivo studies to provide a temporally controlled GDNF source to promote axon growth.

Research paper thumbnail of Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression

Biotechnology and Bioengineering, 2014

Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhan... more Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to reduced motor axons available to reinnervate muscle. To address this issue, we designed a cell-based GDNF expression system that can temporally regulate protein expression using an inducible gene excision mechanism to prevent entrapment at the site of expression. To design this system for regulation of GDNF expression, we transduced two lentiviral vectors, one containing a constitutively active GDNF transgene flanked by two loxP sites, and the other containing a tetracycline-inducible cre transgene along with its constitutively active transactivator, into Schwann cells (SCs). These SCs over-express GDNF, but expression can be suppressed through the administration of tetracycline family antibiotics, such as doxycycline. The engineered SCs produced significantly more GDNF as compared to untransduced controls, as measured by enzyme-linked immunosorbent assay (ELISA). Following doxycycline treatment, these SCs produced significantly lower levels of GDNF and induced less neurite extension as compared to untreated SCs. Engineered SCs treated with doxycycline showed a marked increase in Cre recombinase expression, as visualized by immunohistochemistry (IHC), providing evidence of a mechanism for the observed changes in GDNF expression levels and biological activity. This cell-based GDNF expression system could have potential for future in vivo studies to provide a temporally controlled GDNF source to promote axon growth.

Research paper thumbnail of Characterization and Cost of Intravenous Drug Use–Associated Upper Extremity Infections Requiring Operative Treatment

Plastic & Reconstructive Surgery, 2021

Research paper thumbnail of Targeted Muscle Reinnervation for Partial Hand Amputation

Plastic and Reconstructive Surgery - Global Open, 2020

Research paper thumbnail of Abstract: Implications of Upper Eyelid Ptosis and Blepharodermatochalasis on Fixed Horizontal Forehead Lines

Plastic and Reconstructive Surgery Global Open, 2018

GOALS/PURPOSE: In this age of globalization, patients may seek to have their aesthetic surgeries ... more GOALS/PURPOSE: In this age of globalization, patients may seek to have their aesthetic surgeries performed in foreign countries anticipating significant cost-savings compared to perceived prohibitive out-of-pocket costs in the US. Given the transient nature of their stay in the country of surgery, post-operative follow-up is often sub-optimal. These patients frequently seek care in the US for complications, the burden falling on the US healthcare system. Non-standardization of aseptic surgical facilities and technique leads to an increased risk of infectious complications. Management of these complications represents a resource burden to the healthcare system and poses a clinical danger to the general population. Travel and surgery in uncontrolled settings, with their disparate macro-biomes, facilitates translocation of potentially dangerous infectious microbiota. This study investigated the microbiological data from a series of patients who sustained infectious complications from t...

Research paper thumbnail of An Alternative Dermal Template for Reconstruction of Complex Upper Extremity Wounds

Plastic and Reconstructive Surgery - Global Open, 2021

Research paper thumbnail of Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression

Biotechnology and Bioengineering, 2014

Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhan... more Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to reduced motor axons available to reinnervate muscle. To address this issue, we designed a cell-based GDNF expression system that can temporally regulate protein expression using an inducible gene excision mechanism to prevent entrapment at the site of expression. To design this system for regulation of GDNF expression, we transduced two lentiviral vectors, one containing a constitutively active GDNF transgene flanked by two loxP sites, and the other containing a tetracycline-inducible cre transgene along with its constitutively active transactivator, into Schwann cells (SCs). These SCs over-express GDNF, but expression can be suppressed through the administration of tetracycline family antibiotics, such as doxycycline. The engineered SCs produced significantly more GDNF as compared to untransduced controls, as measured by enzyme-linked immunosorbent assay (ELISA). Following doxycycline treatment, these SCs produced significantly lower levels of GDNF and induced less neurite extension as compared to untreated SCs. Engineered SCs treated with doxycycline showed a marked increase in Cre recombinase expression, as visualized by immunohistochemistry (IHC), providing evidence of a mechanism for the observed changes in GDNF expression levels and biological activity. This cell-based GDNF expression system could have potential for future in vivo studies to provide a temporally controlled GDNF source to promote axon growth.

Research paper thumbnail of Psychiatric Disease after Isolated Traumatic Upper Extremity Amputation

Journal of Hand and Microsurgery

Introduction Psychiatric disease after traumatic limb loss impacts rehabilitation, prosthesis use... more Introduction Psychiatric disease after traumatic limb loss impacts rehabilitation, prosthesis use, and quality of life. The purpose of this study was to evaluate the prevalence of psychiatric disease in civilians after isolated, traumatic upper extremity amputation and determine if any risk factors are associated with developing psychiatric disease. Materials and Methods Demographics, time since injury, mechanism of injury, amputation level, hand affected (dominant vs. nondominant), Bureau of Workers’ Compensation (BWC) status, and prosthesis use were retrospectively reviewed for all patients treated from 2012 to 2017. For patients with an International Statistical Classification of Diseases and Related Health Problems, 10th revision (ICD-10) diagnosed psychiatric disease, the diagnosis and length of treatment were recorded. Patients were grouped by presence or absence of psychiatric diagnosis and data analysis was performed using descriptive statistics, Fisher’s exact test, and rel...

Research paper thumbnail of The Anterolateral Thigh-Medial Femoral Condyle Chimeric Flow-through Flap for Posttraumatic Wrist Arthrodesis

Techniques in Hand & Upper Extremity Surgery

Research paper thumbnail of Impact of interdisciplinary rounds in the spinal cord injury unit on relational coordination for patients with pressure injury: A pilot study

The Journal of Spinal Cord Medicine

Research paper thumbnail of The importance of surgical maneuvers during treatment of frontal migraines (site I): A prospective, randomized cohort study evaluating foraminotomy/fasciotomy, myectomy, and arterectomy

Journal of Plastic, Reconstructive & Aesthetic Surgery

Research paper thumbnail of Horizontal Forehead Lines: A Reflection of Eyelid Ptosis or Blepharodermachalasia

Aesthetic Plastic Surgery

Research paper thumbnail of Anatomical Look Into OnabotulinumtoxinA Injection for Chronic Migraine Headache

Regional Anesthesia and Pain Medicine

Research paper thumbnail of Repair of a Large, Full-Thickness Defect of the Lower Lip Under Local Anesthesia

Research paper thumbnail of Considering the needle length and Body Mass Index as factors that might increase the response rate to onabotulinumtoxinA in Chronic Migraine

Research paper thumbnail of Abstract 91

Plastic and Reconstructive Surgery, 2014

Research paper thumbnail of Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression

Biotechnology and Bioengineering, 2014

Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhan... more Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to reduced motor axons available to reinnervate muscle. To address this issue, we designed a cell-based GDNF expression system that can temporally regulate protein expression using an inducible gene excision mechanism to prevent entrapment at the site of expression. To design this system for regulation of GDNF expression, we transduced two lentiviral vectors, one containing a constitutively active GDNF transgene flanked by two loxP sites, and the other containing a tetracycline-inducible cre transgene along with its constitutively active transactivator, into Schwann cells (SCs). These SCs over-express GDNF, but expression can be suppressed through the administration of tetracycline family antibiotics, such as doxycycline. The engineered SCs produced significantly more GDNF as compared to untransduced controls, as measured by enzyme-linked immunosorbent assay (ELISA). Following doxycycline treatment, these SCs produced significantly lower levels of GDNF and induced less neurite extension as compared to untreated SCs. Engineered SCs treated with doxycycline showed a marked increase in Cre recombinase expression, as visualized by immunohistochemistry (IHC), providing evidence of a mechanism for the observed changes in GDNF expression levels and biological activity. This cell-based GDNF expression system could have potential for future in vivo studies to provide a temporally controlled GDNF source to promote axon growth.

Research paper thumbnail of Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression

Biotechnology and Bioengineering, 2014

Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhan... more Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to reduced motor axons available to reinnervate muscle. To address this issue, we designed a cell-based GDNF expression system that can temporally regulate protein expression using an inducible gene excision mechanism to prevent entrapment at the site of expression. To design this system for regulation of GDNF expression, we transduced two lentiviral vectors, one containing a constitutively active GDNF transgene flanked by two loxP sites, and the other containing a tetracycline-inducible cre transgene along with its constitutively active transactivator, into Schwann cells (SCs). These SCs over-express GDNF, but expression can be suppressed through the administration of tetracycline family antibiotics, such as doxycycline. The engineered SCs produced significantly more GDNF as compared to untransduced controls, as measured by enzyme-linked immunosorbent assay (ELISA). Following doxycycline treatment, these SCs produced significantly lower levels of GDNF and induced less neurite extension as compared to untreated SCs. Engineered SCs treated with doxycycline showed a marked increase in Cre recombinase expression, as visualized by immunohistochemistry (IHC), providing evidence of a mechanism for the observed changes in GDNF expression levels and biological activity. This cell-based GDNF expression system could have potential for future in vivo studies to provide a temporally controlled GDNF source to promote axon growth.