Yuko Kamisaka - Academia.edu (original) (raw)

Papers by Yuko Kamisaka

Comparative Biochemistry and Physiology B, Jun 1, 2008

The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transport... more The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transporter, PepT1. This study evaluates the expression of PepT1 in larval Atlantic cod (Gadus morhua) during the three weeks following the onset of exogenous feeding. Larval Atlantic cod were fed either wild captured zooplankton or enriched rotifers. cDNA was prepared from whole cod larvae preceding first feeding and at 1000 each Tuesday and Thursday for the following three weeks. Spatial and temporal expression patterns of PepT1 mRNA were compared between fish consuming the two prey types using in situ hybridization and quantitative real-time PCR. Results indicated that PepT1 mRNA was expressed prior to the onset of exogenous feeding. In addition, PepT1 was expressed throughout the digestive system except the esophagus and sphincter regions. Expression slightly increased following first-feeding and continued to increase throughout the study for larvae feeding on both prey types. When comparing PepT1 expression in larvae larger than 0.15-mg dry mass with expression levels in larvae prior to feeding, no differences were detected for larvae fed rotifers, but the larvae fed zooplankton had significantly greater PepT1 expression at the larger size. In addition, PepT1 expression in the zooplankton fed larvae larger than 0.15-mg dry mass had significantly greater expression than rotifer fed larvae of a similar weight. Switching prey types did not affect PepT1 expression. These results indicate that Atlantic cod PepT1 expression was slightly different relative to dietary treatment during the three weeks following first-feeding. In addition, PepT1 may play an important role in the larval nutrition since it is widely expressed in the digestive tract.

Journal of Fish Biology, Sep 1, 2009

One of the main gastrointestinal hormones, cholecystokinin (CCK), was studied in order to advance... more One of the main gastrointestinal hormones, cholecystokinin (CCK), was studied in order to advance understanding of the control of the digestive process in Atlantic cod Gadus morhua larvae after onset of first feeding. Larvae were fed either natural zooplankton or enriched rotifers in similar rearing systems and sampled from hatching to 22 days post-hatch (dph). CCK was visualized by immunohistochemistry and the first CCK-immunoreactive (IR) cells were detected at 8 dph corresponding to 6 days after first feeding. The CCK-IR cells were mostly found in the anterior midgut, and the number of CCK-IR cells was lower in the posterior midgut. They were also present in the hindgut of some of the larvae, but not in the foregut. No clear differences were found in the ontogenetic appearance and the distribution pattern of CCK-IR cells between the two dietary treatments. This indicates that the onset of CCK production in the gut as well as the spatial distribution of the CCK-IR cells is not differentially affected by these diets. To what extent the hormone production itself is influenced by dietary factors needs to be studied by more sensitive methods.

Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, Jul 1, 2000

Post-larval halibut, Hippoglossus hippoglossus (56 days after first feeding; 23 mg dw) were tube ... more Post-larval halibut, Hippoglossus hippoglossus (56 days after first feeding; 23 mg dw) were tube fed diets containing free amino acids (FAA-diet), peptides (Pept-diet) and protein (Prot-diet) in order to compare kinetic of transfer from gut into the liver and the body carcass. The test diets were prepared with approximately equal concentrations of amino acids. The Protdiet diet consisted of BSA (added IaC-BSA); The Pept-diet was the protein BSA digested with trypsin; and the FAA-diet comprised an amino acid mixture based on BSA composition added 14C-lysine. The larvae had empty guts (unfed for 24 hrs at 12 °C, 32 g/L salinity, constant light) and were anaesthetised in 0.1 g/L MS 222 for 2 minutes previous to tube feeding. Approximate 300 nL of the diet were delivered inside the gut lumen of individual larvae with a micro-injector; this volume equals about 2,2 jag amino acids. Groups of five to ten larvae were injected for each dietary treatment and sampled after a set time (0, 0.25, 0.5, 1, 2, 4, 8 and 24 h). The larvae were killed by spinal cord lesion and thereafter the digestive tract and the liver were dissected out. Solubles and precipitates (6% cold trichloroacetic acid) were separated for each compartment. All samples were analysed by liquid scintillation counting.

Nordisk Barnehageforskning, Apr 26, 2022

Nordisk barnehageforskning

Praksisperioden som inngår i barnehagelærerutdanningen er en viktig arena der studentene kan se s... more Praksisperioden som inngår i barnehagelærerutdanningen er en viktig arena der studentene kan se sammenhengen mellom teori og praksis og reflektere over egen praksis. Studenter i emnet natur, helse og rørsle i barnehagelærerutdanningen har gjennomført en praksisoppgave der de skulle observere alle måltider og håndvask før måltid i sin praksisbarnehage i en uke. Resultatene av praksisoppgaven ble samlet og brukt i undervisning etter at de var ferdig med praksisen, for å diskutere variasjon av mattilbud og håndvaskrutiner mellom barnehagene. Formålet med denne artikkelen er å undersøke hvilke læringsutbytter studentene selv mener at de har fått gjennom praksisoppgaven. Totalt 45 studenter svarte på en spørreundersøkelse, og to studenter var videre med på forskningsintervju. Resultatene viser at studentene har fått grunnleggende kompetanse om kosthold, hygiene og måltidets funksjon i barnehagen, de har blitt trygge på å referere til nødvendige rammeverk rundt måltid og blitt mer oppmerk...

Nordisk barnehageforskning

Formålet med denne studien var å få oppdatert innsikt i mattilbudet i barnehager og håndhygiene i... more Formålet med denne studien var å få oppdatert innsikt i mattilbudet i barnehager og håndhygiene i forbindelse med måltidene. Studien er basert på en praksisoppgave der barnehagelærerstudenter har observert måltidene i fem praksisperioder i 2016–2018, og vi har observasjonsdata fra totalt 120 studenter. Studentene observerte om praksisbarnehagen serverte mat eller om barna hadde medbrakt mat, samt hvilken mat som ble servert i løpet av en uke. Et annet fokus var håndhygiene, og det ble registrert om barna vasket hendene før måltidene eller ikke. Resultatene viser at de fleste barnehagene serverte brødmåltid tre ganger og varmmåltid to ganger til lunsj i løpet av uken. Som pålegg var hvitost og leverpostei mest vanlig. Egg og fiskepålegg var sjelden på bordet, med unntak av makrell i tomat og kaviar. Av varmmåltid var pølser, fiskekaker, havregrøt, ostesmørbrød og forskjellige typer suppe mest vanlig. Frukt og grønt ble ikke servert så ofte ved brødmåltid, men over 90 prosent av barne...

Journal of Experimental Biology, 2007

SUMMARY A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type o... more SUMMARY A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type oligopeptide transporter has been cloned. This cDNA(named codPepT1) was 2838 bp long, with an open reading frame of 2190 bp encoding a putative protein of 729 amino acids. Comparison of the predicted Atlantic cod PepT1 protein with zebrafish, bird and mammalian orthologs allowed detection of many structural features that are highly conserved among all the vertebrate proteins analysed, including (1) a larger than expected area of hydrophobic amino acids in close proximity to the N terminus; (2) a single highly conserved cAMP/cGMP-dependent protein kinase phosphorylation motif; (3) a large N-glycosylation-rich region within the large extracellular loop; and (4) a conserved and previously undescribed stretch of 8–12 amino acid residues within the large extracellular loop. Expression analysis at the mRNA level indicated that Atlantic cod PepT1 is mainly expressed at intestinal level, but that...

General and Comparative Endocrinology, 2001

The appearance and distribution of cholecystokinin (CCK)-producing cells were investigated in the... more The appearance and distribution of cholecystokinin (CCK)-producing cells were investigated in the digestive tract of developing larvae of cultured Atlantic halibut, Hippoglossus hippoglossus. The CCK-producing cells were detected immunohistochemically, by use of a primary antiserum against CCK cloned for the Japanese flounder, Paralichthys olivaceus. No CCK-immunoreactive (IR) cells were detected in first-feeding larvae (33 days after hatching, DAH). Forty-five DAH or 12 days after first feeding, there were a few scattered CCK-IR cells in the epithelium of the anterior midgut in about 30% of the examined larvae. All larvae older than 52 DAH had CCK-IR cells in the anterior midgut, particularly frequent in the most anterior region adjacent to the pyloric caeca. No CCK-IR cells were detected in the foregut, the hindgut, or the midgut posterior to the first curvature. The CCK-IR cells spanned the intestinal epithelium from the basal lamina to the lumen and were triangular in shape, with the nucleus in the basal part and a thin apex toward the lumen. The mechanisms controlling release of bile, pancreatic enzymes, and peristalsis during the 12 days between first feeding and the first detection of CCK-IR cells remain to be clarified.

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2008

The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transport... more The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transporter, PepT1. This study evaluates the expression of PepT1 in larval Atlantic cod (Gadus morhua) during the three weeks following the onset of exogenous feeding. Larval Atlantic cod were fed either wild captured zooplankton or enriched rotifers. cDNA was prepared from whole cod larvae preceding first feeding and at 1000 each Tuesday and Thursday for the following three weeks. Spatial and temporal expression patterns of PepT1 mRNA were compared between fish consuming the two prey types using in situ hybridization and quantitative real-time PCR. Results indicated that PepT1 mRNA was expressed prior to the onset of exogenous feeding. In addition, PepT1 was expressed throughout the digestive system except the esophagus and sphincter regions. Expression slightly increased following first-feeding and continued to increase throughout the study for larvae feeding on both prey types. When comparing PepT1 expression in larvae larger than 0.15-mg dry mass with expression levels in larvae prior to feeding, no differences were detected for larvae fed rotifers, but the larvae fed zooplankton had significantly greater PepT1 expression at the larger size. In addition, PepT1 expression in the zooplankton fed larvae larger than 0.15-mg dry mass had significantly greater expression than rotifer fed larvae of a similar weight. Switching prey types did not affect PepT1 expression. These results indicate that Atlantic cod PepT1 expression was slightly different relative to dietary treatment during the three weeks following first-feeding. In addition, PepT1 may play an important role in the larval nutrition since it is widely expressed in the digestive tract.

doi:10.1242/jeb.007898 Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning,... more doi:10.1242/jeb.007898 Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects

ontogeny were reconstructed from histological sections of Atlantic cod larvae. The 3D models clea... more ontogeny were reconstructed from histological sections of Atlantic cod larvae. The 3D models clearly visualize the following features: folding of the gut rotation; subdivision of digestive tract into foregut, midgut, and hindgut by sphincters; development of stomach and pyloric caeca from 39 dph; location of entrances of bile and pancreatic ducts in the medial plane of the anterior midgut; ontogeny of pancreas from a compact organ to an elongated and bran-ched (but not diffuse) organ along the posterior midgut; one dominant islet of Langerhans until 39 dph and several smaller satellite islets also visible from 53 dph; the rela-tively large volume of the anterior midgut that probably increases residence time of ingested food mixing with secretions from pancreas and bile. Calculated volumes of each digestive organ demonstrate allometric changes dur-ing ontogeny. Interactive 3D models are available as QuickTime format downloadable files.

... what are the resulting constraints on amino acid absorption and the implications for first ..... more ... what are the resulting constraints on amino acid absorption and the implications for first ... recently been reported using formulated diets for first feeding seabass Dicentrarchus labrax [Cahu and ... halibut, we also demonstrated lower absorption rates from the digestive tract of protein ...

Functional modifications associated with nogenesis during Conclusions: The functional specializat... more Functional modifications associated with nogenesis during Conclusions: The functional specialization of the GI-tract was not exclusive to metamorphosis, and its osmoregulatory Gomes et al. BMC Developmental Biology 2014, 14:11

Peptides, 2005

The mRNA of the peptide hormone cholecystokinin (CCK) was isolated from juvenile Atlantic herring... more The mRNA of the peptide hormone cholecystokinin (CCK) was isolated from juvenile Atlantic herring, Clupea harengus, by RT-PCR. The open reading frame encodes a 137 amino acid-long precursor protein. The peptide sequence of herring CCK-8, DYMGWMDF, is identical to that of higher vertebrates and elasmobranchs, and contains methionine in the sixth position from the C-terminus, which has not been reported previously in teleosts. Expression analysis by in situ hybridization shows that positive endocrine-like cells were mainly located in the pyloric caeca and to a less extent in the rectum of the juvenile. A few positive cells were also found in the pyloric portion of the stomach and the intestine. CCK cells were present in all the larvae examined from the day of hatching onwards. Although the CCK cells were scattered throughout the whole midgut, no signals were detected in either the foregut or the hindgut. Since herring larvae have a straight gut, the distribution pattern of CCK cells s...

Comparative Biochemistry and Physiology B, Jun 1, 2008

The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transport... more The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transporter, PepT1. This study evaluates the expression of PepT1 in larval Atlantic cod (Gadus morhua) during the three weeks following the onset of exogenous feeding. Larval Atlantic cod were fed either wild captured zooplankton or enriched rotifers. cDNA was prepared from whole cod larvae preceding first feeding and at 1000 each Tuesday and Thursday for the following three weeks. Spatial and temporal expression patterns of PepT1 mRNA were compared between fish consuming the two prey types using in situ hybridization and quantitative real-time PCR. Results indicated that PepT1 mRNA was expressed prior to the onset of exogenous feeding. In addition, PepT1 was expressed throughout the digestive system except the esophagus and sphincter regions. Expression slightly increased following first-feeding and continued to increase throughout the study for larvae feeding on both prey types. When comparing PepT1 expression in larvae larger than 0.15-mg dry mass with expression levels in larvae prior to feeding, no differences were detected for larvae fed rotifers, but the larvae fed zooplankton had significantly greater PepT1 expression at the larger size. In addition, PepT1 expression in the zooplankton fed larvae larger than 0.15-mg dry mass had significantly greater expression than rotifer fed larvae of a similar weight. Switching prey types did not affect PepT1 expression. These results indicate that Atlantic cod PepT1 expression was slightly different relative to dietary treatment during the three weeks following first-feeding. In addition, PepT1 may play an important role in the larval nutrition since it is widely expressed in the digestive tract.

Journal of Fish Biology, Sep 1, 2009

One of the main gastrointestinal hormones, cholecystokinin (CCK), was studied in order to advance... more One of the main gastrointestinal hormones, cholecystokinin (CCK), was studied in order to advance understanding of the control of the digestive process in Atlantic cod Gadus morhua larvae after onset of first feeding. Larvae were fed either natural zooplankton or enriched rotifers in similar rearing systems and sampled from hatching to 22 days post-hatch (dph). CCK was visualized by immunohistochemistry and the first CCK-immunoreactive (IR) cells were detected at 8 dph corresponding to 6 days after first feeding. The CCK-IR cells were mostly found in the anterior midgut, and the number of CCK-IR cells was lower in the posterior midgut. They were also present in the hindgut of some of the larvae, but not in the foregut. No clear differences were found in the ontogenetic appearance and the distribution pattern of CCK-IR cells between the two dietary treatments. This indicates that the onset of CCK production in the gut as well as the spatial distribution of the CCK-IR cells is not differentially affected by these diets. To what extent the hormone production itself is influenced by dietary factors needs to be studied by more sensitive methods.

Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, Jul 1, 2000

Post-larval halibut, Hippoglossus hippoglossus (56 days after first feeding; 23 mg dw) were tube ... more Post-larval halibut, Hippoglossus hippoglossus (56 days after first feeding; 23 mg dw) were tube fed diets containing free amino acids (FAA-diet), peptides (Pept-diet) and protein (Prot-diet) in order to compare kinetic of transfer from gut into the liver and the body carcass. The test diets were prepared with approximately equal concentrations of amino acids. The Protdiet diet consisted of BSA (added IaC-BSA); The Pept-diet was the protein BSA digested with trypsin; and the FAA-diet comprised an amino acid mixture based on BSA composition added 14C-lysine. The larvae had empty guts (unfed for 24 hrs at 12 °C, 32 g/L salinity, constant light) and were anaesthetised in 0.1 g/L MS 222 for 2 minutes previous to tube feeding. Approximate 300 nL of the diet were delivered inside the gut lumen of individual larvae with a micro-injector; this volume equals about 2,2 jag amino acids. Groups of five to ten larvae were injected for each dietary treatment and sampled after a set time (0, 0.25, 0.5, 1, 2, 4, 8 and 24 h). The larvae were killed by spinal cord lesion and thereafter the digestive tract and the liver were dissected out. Solubles and precipitates (6% cold trichloroacetic acid) were separated for each compartment. All samples were analysed by liquid scintillation counting.

Nordisk Barnehageforskning, Apr 26, 2022

Nordisk barnehageforskning

Praksisperioden som inngår i barnehagelærerutdanningen er en viktig arena der studentene kan se s... more Praksisperioden som inngår i barnehagelærerutdanningen er en viktig arena der studentene kan se sammenhengen mellom teori og praksis og reflektere over egen praksis. Studenter i emnet natur, helse og rørsle i barnehagelærerutdanningen har gjennomført en praksisoppgave der de skulle observere alle måltider og håndvask før måltid i sin praksisbarnehage i en uke. Resultatene av praksisoppgaven ble samlet og brukt i undervisning etter at de var ferdig med praksisen, for å diskutere variasjon av mattilbud og håndvaskrutiner mellom barnehagene. Formålet med denne artikkelen er å undersøke hvilke læringsutbytter studentene selv mener at de har fått gjennom praksisoppgaven. Totalt 45 studenter svarte på en spørreundersøkelse, og to studenter var videre med på forskningsintervju. Resultatene viser at studentene har fått grunnleggende kompetanse om kosthold, hygiene og måltidets funksjon i barnehagen, de har blitt trygge på å referere til nødvendige rammeverk rundt måltid og blitt mer oppmerk...

Nordisk barnehageforskning

Formålet med denne studien var å få oppdatert innsikt i mattilbudet i barnehager og håndhygiene i... more Formålet med denne studien var å få oppdatert innsikt i mattilbudet i barnehager og håndhygiene i forbindelse med måltidene. Studien er basert på en praksisoppgave der barnehagelærerstudenter har observert måltidene i fem praksisperioder i 2016–2018, og vi har observasjonsdata fra totalt 120 studenter. Studentene observerte om praksisbarnehagen serverte mat eller om barna hadde medbrakt mat, samt hvilken mat som ble servert i løpet av en uke. Et annet fokus var håndhygiene, og det ble registrert om barna vasket hendene før måltidene eller ikke. Resultatene viser at de fleste barnehagene serverte brødmåltid tre ganger og varmmåltid to ganger til lunsj i løpet av uken. Som pålegg var hvitost og leverpostei mest vanlig. Egg og fiskepålegg var sjelden på bordet, med unntak av makrell i tomat og kaviar. Av varmmåltid var pølser, fiskekaker, havregrøt, ostesmørbrød og forskjellige typer suppe mest vanlig. Frukt og grønt ble ikke servert så ofte ved brødmåltid, men over 90 prosent av barne...

Journal of Experimental Biology, 2007

SUMMARY A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type o... more SUMMARY A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type oligopeptide transporter has been cloned. This cDNA(named codPepT1) was 2838 bp long, with an open reading frame of 2190 bp encoding a putative protein of 729 amino acids. Comparison of the predicted Atlantic cod PepT1 protein with zebrafish, bird and mammalian orthologs allowed detection of many structural features that are highly conserved among all the vertebrate proteins analysed, including (1) a larger than expected area of hydrophobic amino acids in close proximity to the N terminus; (2) a single highly conserved cAMP/cGMP-dependent protein kinase phosphorylation motif; (3) a large N-glycosylation-rich region within the large extracellular loop; and (4) a conserved and previously undescribed stretch of 8–12 amino acid residues within the large extracellular loop. Expression analysis at the mRNA level indicated that Atlantic cod PepT1 is mainly expressed at intestinal level, but that...

General and Comparative Endocrinology, 2001

The appearance and distribution of cholecystokinin (CCK)-producing cells were investigated in the... more The appearance and distribution of cholecystokinin (CCK)-producing cells were investigated in the digestive tract of developing larvae of cultured Atlantic halibut, Hippoglossus hippoglossus. The CCK-producing cells were detected immunohistochemically, by use of a primary antiserum against CCK cloned for the Japanese flounder, Paralichthys olivaceus. No CCK-immunoreactive (IR) cells were detected in first-feeding larvae (33 days after hatching, DAH). Forty-five DAH or 12 days after first feeding, there were a few scattered CCK-IR cells in the epithelium of the anterior midgut in about 30% of the examined larvae. All larvae older than 52 DAH had CCK-IR cells in the anterior midgut, particularly frequent in the most anterior region adjacent to the pyloric caeca. No CCK-IR cells were detected in the foregut, the hindgut, or the midgut posterior to the first curvature. The CCK-IR cells spanned the intestinal epithelium from the basal lamina to the lumen and were triangular in shape, with the nucleus in the basal part and a thin apex toward the lumen. The mechanisms controlling release of bile, pancreatic enzymes, and peristalsis during the 12 days between first feeding and the first detection of CCK-IR cells remain to be clarified.

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2008

The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transport... more The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transporter, PepT1. This study evaluates the expression of PepT1 in larval Atlantic cod (Gadus morhua) during the three weeks following the onset of exogenous feeding. Larval Atlantic cod were fed either wild captured zooplankton or enriched rotifers. cDNA was prepared from whole cod larvae preceding first feeding and at 1000 each Tuesday and Thursday for the following three weeks. Spatial and temporal expression patterns of PepT1 mRNA were compared between fish consuming the two prey types using in situ hybridization and quantitative real-time PCR. Results indicated that PepT1 mRNA was expressed prior to the onset of exogenous feeding. In addition, PepT1 was expressed throughout the digestive system except the esophagus and sphincter regions. Expression slightly increased following first-feeding and continued to increase throughout the study for larvae feeding on both prey types. When comparing PepT1 expression in larvae larger than 0.15-mg dry mass with expression levels in larvae prior to feeding, no differences were detected for larvae fed rotifers, but the larvae fed zooplankton had significantly greater PepT1 expression at the larger size. In addition, PepT1 expression in the zooplankton fed larvae larger than 0.15-mg dry mass had significantly greater expression than rotifer fed larvae of a similar weight. Switching prey types did not affect PepT1 expression. These results indicate that Atlantic cod PepT1 expression was slightly different relative to dietary treatment during the three weeks following first-feeding. In addition, PepT1 may play an important role in the larval nutrition since it is widely expressed in the digestive tract.

doi:10.1242/jeb.007898 Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning,... more doi:10.1242/jeb.007898 Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects

ontogeny were reconstructed from histological sections of Atlantic cod larvae. The 3D models clea... more ontogeny were reconstructed from histological sections of Atlantic cod larvae. The 3D models clearly visualize the following features: folding of the gut rotation; subdivision of digestive tract into foregut, midgut, and hindgut by sphincters; development of stomach and pyloric caeca from 39 dph; location of entrances of bile and pancreatic ducts in the medial plane of the anterior midgut; ontogeny of pancreas from a compact organ to an elongated and bran-ched (but not diffuse) organ along the posterior midgut; one dominant islet of Langerhans until 39 dph and several smaller satellite islets also visible from 53 dph; the rela-tively large volume of the anterior midgut that probably increases residence time of ingested food mixing with secretions from pancreas and bile. Calculated volumes of each digestive organ demonstrate allometric changes dur-ing ontogeny. Interactive 3D models are available as QuickTime format downloadable files.

... what are the resulting constraints on amino acid absorption and the implications for first ..... more ... what are the resulting constraints on amino acid absorption and the implications for first ... recently been reported using formulated diets for first feeding seabass Dicentrarchus labrax [Cahu and ... halibut, we also demonstrated lower absorption rates from the digestive tract of protein ...

Functional modifications associated with nogenesis during Conclusions: The functional specializat... more Functional modifications associated with nogenesis during Conclusions: The functional specialization of the GI-tract was not exclusive to metamorphosis, and its osmoregulatory Gomes et al. BMC Developmental Biology 2014, 14:11

Peptides, 2005

The mRNA of the peptide hormone cholecystokinin (CCK) was isolated from juvenile Atlantic herring... more The mRNA of the peptide hormone cholecystokinin (CCK) was isolated from juvenile Atlantic herring, Clupea harengus, by RT-PCR. The open reading frame encodes a 137 amino acid-long precursor protein. The peptide sequence of herring CCK-8, DYMGWMDF, is identical to that of higher vertebrates and elasmobranchs, and contains methionine in the sixth position from the C-terminus, which has not been reported previously in teleosts. Expression analysis by in situ hybridization shows that positive endocrine-like cells were mainly located in the pyloric caeca and to a less extent in the rectum of the juvenile. A few positive cells were also found in the pyloric portion of the stomach and the intestine. CCK cells were present in all the larvae examined from the day of hatching onwards. Although the CCK cells were scattered throughout the whole midgut, no signals were detected in either the foregut or the hindgut. Since herring larvae have a straight gut, the distribution pattern of CCK cells s...