Yutaka Jitsuyama - Academia.edu (original) (raw)

Papers by Yutaka Jitsuyama

American Journal of Plant Sciences, 2017

Excess soil moisture induces hypoxic conditions and causes waterlogging injury in soybean [Glycin... more Excess soil moisture induces hypoxic conditions and causes waterlogging injury in soybean [Glycine max (L.) Merr.]. This study investigated the mechanism underlying the development of waterlogging injury. Nine Japanese soybean cultivars with varying degrees of waterlogging tolerance were grown in a hydroponic system for 14 days under hypoxic conditions. Shoot and root biomasses and root hydraulic conductivity were measured at an early vegetative stage for plants under control and hypoxic conditions. Root morphological traits and intramembrane aquaporin proteins were also analyzed. The tolerance of each cultivar to field waterlogging was based on biomass changes induced by the hypoxia treatment. Root hydraulic conductivity responses to hypoxia were associated with changes in total dry weight, leaf dry weight, and leaf area. The effects of hypoxic conditions on root hydraulic conductivity were also represented by the changes in root morphology, such as total root length, thick-root length, and number of root tips. Additionally, a 32.3 kDa aquaporin-like protein seemed to regulate root hydraulic conductivity. Our results from a hydroponic culture suggest that the soybean cultivar-specific responses to hypoxic conditions in the rhizosphere reflect fluctuations in hydraulic conductivity related to root morphological or qualitative changes.

American Journal of Plant Sciences, 2013

To investigate the soybean (Glycine max Merr.) wet endurance, and the affect of the maturity and ... more To investigate the soybean (Glycine max Merr.) wet endurance, and the affect of the maturity and the ambient temperature to the response, the plantlet in growth stage ranged from R1 to R2 of 8 Japanese soybean cultivars which characterized as various wet endurance in a past report were cultured under hypoxic-hydroponic condition for a month. Two experiments at different periods differed significantly in temperature of air and hydroponic solution, but the oxygenic condition were similar each experiments, as aimed for. And also, control and hypoxia treatments significantly differed in dissolved oxygenic concentration in each experiments. The hypoxic condition at higher temperature induced the reduction of survival of plantlet up to 70%. At higher temperature, the survival rate of late maturity types "Yuzuru" and "Shirotsurunoko" were decreased significantly in hypoxic condition than in control. Similarly, the shoot dry matters of them were also decreased significantly in hypoxia at higher temperature. The tendency of the decreasing in hypoxia was remarkable in the pod dry matter, and the symptoms were shown also at the lower temperature. From these results, soybean's hypoxic tolerant may be reflected with the wet endurance, the process of hypoxic damages can be divided to sudden death symptom and biomass decreasing, and the mechanisms of hypoxic tolerance might be affected strongly by ambient temperature, and absolutely controlled with the genetic background.

Canadian Journal of Plant Science, 2015

Jitsuyama, Y. 2015. Morphological root responses of soybean to rhizosphere hypoxia reflect waterl... more Jitsuyama, Y. 2015. Morphological root responses of soybean to rhizosphere hypoxia reflect waterlogging tolerance. Can. J. Plant Sci. 95: 999–1005. Excess soil moisture induces hypoxia, causing waterlogging injury in soybeans [Glycine max (L.) Merr.]. Twelve Japanese soybean cultivars with varying hypoxia tolerance were used. Of these, 11 (all but Hayahikari) were evaluated for waterlogging tolerance using a scaled index with data from previous studies. To investigate hypoxic responses, cultivars were grown under hydroponic conditions for 2 wk a year for 2 yr, with aerobic or hypoxic oxygen concentrations artificially maintained in the rhizosphere. Hypoxic responses (measured as plant dry weight and root morphology) were assessed at the early vegetative stage. The effects of hypoxic treatment on root dry weight were significant, and the effect of year on soybean dry weight was not significant. The change in root dry weight, and particularly, in coarse root length, was significantly ...

PLOS ONE, 2015

Physical dormancy, a structural feature of the seed coat known as hard seededness, is an importan... more Physical dormancy, a structural feature of the seed coat known as hard seededness, is an important characteristic for adaptation of plants against unstable and unpredictable environments. To dissect the molecular basis of qHS1, a quantitative trait locus for hard seededness in soybean (Glycine max (L) Merr.), we developed a near-isogenic line (NIL) of a permeable (soft-seeded) cultivar, Tachinagaha, containing a hard-seed allele from wild soybean (G. soja) introduced by successive backcrossings. The hard-seed allele made the seed coat of Tachinagaha more rigid by increasing the amount of β-1,4-glucans in the outer layer of palisade cells of the seed coat on the dorsal side of seeds, known to be a point of entrance of water. Fine-mapping and subsequent expression and sequencing analyses revealed that qHS1 encodes an endo-1,4-β-glucanase. A single-nucleotide polymorphism (SNP) introduced an amino acid substitution in a substrate-binding cleft of the enzyme, possibly reducing or eliminating its affinity for substrates in permeable cultivars. Introduction of the genomic region of qHS1 from the impermeable (hard-seeded) NIL into the permeable cultivar Kariyutaka resulted in accumulation of β-1,4-glucan in the outer layer of palisade cells and production of hard seeds. The SNP allele found in the NIL was further associated with the occurrence of hard seeds in soybean cultivars of various origins. The findings of this and previous studies may indicate that qHS1 is involved in the accumulation of β-1,4glucan derivatives such as xyloglucan and/or β-(1,3)(1,4)-glucan that reinforce the impermeability of seed coats in soybean.

Seed Science Research, 2014

Soybean seeds are prone to flooding injury just after planting if they are exposed to excess mois... more Soybean seeds are prone to flooding injury just after planting if they are exposed to excess moisture. We investigated the flooding tolerance of soybean seeds to clarify the relationship between flooding injury and imbibition, to identify factors that could provide fundamental information for the breeding of flooding-tolerant cultivars. Seven cultivars were used for this study. The seeds were geminated in a paper towel after a flooding treatment and then evaluated for survival. The imbibition of submerged seeds (passive imbibition) was evaluated as the seed fresh weight change during the flooding process. The imbibition of seeds with capillary effect (capillary imbibition) was measured by the bottom water supply method. Furthermore, the conductivity of the seed coat and seed osmolarity were measured. After 3 h of flooding, significant differences in survival were detected among cultivars. In addition, the passive imbibition during 10–30 min of flooding, and the capillary imbibition ...

Scientia Horticulturae, 2022

Abstract Fruits of the genus Rosa plants are called rose hips. The common hips of R. canina are w... more Abstract Fruits of the genus Rosa plants are called rose hips. The common hips of R. canina are well known as a rich source of antioxidants like ascorbic acid and polyphenols. To investigate availability in the hips in Rosa spp., wild Rosa hips originating from East Asia, i.e. R. acicularis, R. davurica, R. multiflora and R. rugosa were evaluated in terms of the content of antioxidants and antioxidant ability in the hydrophilic extracts. The hips from R. glauca originating from south Europe and its interspecific hybrids (‘Kitaayaka’ and ‘Consared’), and purchased R. canina hips were also examined. In addition to the colorimetric detections of DPPH and ORAC, ESR–ST methods were employed for evaluating antioxidant ability, which can determine scavenging activities against naturally–occurring ROS i.e. superoxide anion radical (•O2–), hydroxyl radical (HO•), alkoxyl radical (RO•) and singlet oxygen (1O2), individually. The hips of R. davurica and ‘Consared’ showed quite high values in both the total content of ASA plus DHA (40.8–103.1 g/kg DW) and total polyphenols (119.2–161.5 g quercetin eq./kg DW) regardless of the years collected. They also had high antioxidant activities against each radical compared to other rose hips, and thus their antioxidant ability seems multiple. Both ASA and polyphenols could scavenge radicals of ROO• and 1O2, since significant correlations (P

Scientia Horticulturae, 2021

Abstract In a study of parthenocarpy induction after intergeneric pollination between plants belo... more Abstract In a study of parthenocarpy induction after intergeneric pollination between plants belonging to nine cucurbit genera (Citrullus, Lagenaria, Benincasa, Cucumis, Diplocyclos, Coccinia, Luffa, Trichosanthes and Momordica), we found that parthenocarpy was induced after pollination with pollen from another genus. Parthenocarpy was induced in female flowers of watermelon only with pollen of bottle gourd; the two species are considered to be the most closely related among species in tribe Benincaseae, and bottle gourd pollens have specific factors that induce fruit set of watermelon. In other species, parthenocarpy was induced not only between closely related genera but also between distantly related genera. Parthenocarpy was not induced in female flowers of melon and bitter melon that received pollen from different species. From observations of pollen tube elongation in the pistil, we found that elongation was not necessarily related to parthenocarpy induction in some species. Most species produced fruit set only when pollinated, suggesting that pollen germination and pollen tube elongation generate a signal(s) to induce fruit enlargement. We consider that reception and transduction of the signal(s) depends on the origin of pistil and pollen and that the factors involved in the induction of parthenocarpy are present in both male and female organs. The phenomenon of parthenocarpy after pollination with pollen from a different genus seems to be conserved widely in Cucurbitaceae, but is not known in other plant families. The factors involved in this unique parthenocarpy in Cucurbitaceae are discussed.

The Horticulture Journal, 2019

An excess or lack of soil moisture are significant abiotic stresses that reduce the average yield... more An excess or lack of soil moisture are significant abiotic stresses that reduce the average yield for vegetable crops worldwide. The responses of 'Natsunoshun', a processing tomato cultivar, to water stress at three growth stages, first flower differentiation, first flowering, and fruit development, were investigated over a two-year period. The year effect on yield was not significant; however, the growth stage and the type of water stress at a particular growth stage affected yield significantly. Either an excess or lack of soil moisture after the first flowering stage were significant in reducing yield. The decrease was related to the average weight of the fruit rather than the number per plant. Under dry conditions, fruit number was the same as control plants, but there was a decrease in the reddish mature fruit ratio. On the other hand, under wet conditions plant biomass decreased, especially in the roots, even if there was a transition from wet to dry conditions. We conclude that excessive soil moisture during the first flowering stage produces slower CGR and decreased biomass in the roots, which we believe is the reason for the yield decline.

Scientia Horticulturae, 2019

Sugar accumulation and activities of enzymes involved in fructan dynamics from seedling to bulb f... more Sugar accumulation and activities of enzymes involved in fructan dynamics from seedling to bulb formation in onion (Allium cepa L.

Cryobiology and Cryotechnology, 1995

Japanese Journal of Crop Science, 2008

Plant and Soil, 2004

Improvement of root penetration (RP) ability in durum wheat (Triticum turgidum L. var. durum) is ... more Improvement of root penetration (RP) ability in durum wheat (Triticum turgidum L. var. durum) is an important breeding target to reduce yield losses due to soil compaction and drought. This study investigated the genotypic difference of RP ability in durum wheat of seven Ethiopian landraces (ET genotypes), which were presumed to adapt to soil compaction, and 17 genotypes bred in North America (NA genotypes). The pregerminated seed was planted in a pot with four paraffin-Vaseline (PV) discs of hardness 0.12, 0.24, 0.50 and 0.73 MPa. After eight weeks from planting, each plant was sampled and the number of roots penetrating through the PV discs was counted. No genotype penetrated through the PV disc of 0.73 MPa hardness. The number of roots penetrating through the PV disc of 0.50 MPa hardness showed a significant difference among the genotypes: highest in an ET genotype and lowest in a NA genotype. The mean value was also significantly higher in ET genotypes than in NA genotypes, and it significantly correlated with root weight density below the disc and plant height. The mean root diameter and root weight density above the disc showed no significant difference between NA and ET genotypes. Our results indicate that a large genotypic difference exists for RP ability in durum wheat, and that ET genotypes have a higher RP ability than NA genotypes. Abbreviations: RP-root penetration; ET genotypes-Ethiopian landraces; NA genotypes-North American varieties; PV-the mixture of paraffin and Vaseline; 20PV, 30PV, 40PV or 50PV-the mixture containing 200, 300, 400 or 500 g paraffin (800, 700, 600 or 500 g Vaseline) per kg mixture, respectively.